A Photometric Method for Recording Hoof Diseases in Cattle,with Special Reference to Haemorrhages of the Sole

After cleaning and trimming, the sole surface and interdigital space of the hooves of cows are photographed in a standardized way. The colour slides are projected on to a screen and the haemorrhages of the sole (reflecting Pododermatitis aseptica diffusa) and the lesions of Dermatitis interdigitalis and Erosio ungulae are scored according to their severity and extent by 2 people who do not know the identity of the cows. In a repeatability test the correlation coefficients between 2 assessments of the haemorrhages in the sole ranged between 0.78 and 0.88 for assessments of the front hooves, the hind hooves and all the hooves together.     

A Method for Recording the Motor Activity of the Reticulum in Cattle

A method for recording the motility of the reticulum in normal cattle has been devised. The method is based on measurement of the pressure variations occurring in connection with the reticular contractions. The pressure is transferred through open, water-filled catheters via a pressure transducer to an electromanometer, from which it is recorded with the aid of a mingograf. Mean values for the interval, duration and amplitude of the reticular contractions in 10 normal cows are given. The method permits recording in intact animals without any preliminary measures, and can therefore be used in clinical cases.     

The Accuracy of Genomic Selection in Norwegian Red Cattle Assessed by Cross-Validation

Genomic Selection (GS) is a newly developed tool for the estimation of breeding values for quantitative traits through the use of dense markers covering the whole genome. For a successful application of GS, accuracy of the prediction of genomewide breeding value (GW-EBV) is a key issue to consider. Here we investigated the accuracy and possible bias of GW-EBV prediction, using real bovine SNP genotyping (18,991 SNPs) and phenotypic data of 500 Norwegian Red bulls. The study was performed on milk yield, fat yield, protein yield, first lactation mastitis traits, and calving ease. Three methods, best linear unbiased prediction (G-BLUP), Bayesian statistics (BayesB), and a mixture model approach (MIXTURE), were used to estimate marker effects, and their accuracy and bias were estimated by using cross-validation. The accuracies of the GW-EBV prediction were found to vary widely between 0.12 and 0.62. G-BLUP gave overall the highest accuracy. We observed a strong relationship between the accuracy of the prediction and the heritability of the trait. GW-EBV prediction for production traits with high heritability achieved higher accuracy and also lower bias than health traits with low heritability. To achieve a similar accuracy for the health traits probably more records will be needed.GENOMIC selection (GS) is a new technology that is expected to revolutionize animal breeding. It is distinct from traditional selection methods where phenotype and pedigree information is combined to predict breeding values and where at least one source is necessary for a prediction. Estimation of GS breeding value is based on the estimation of marker effects covering the whole genome and combines these estimates with the marker genotypes to obtain breeding value estimates. Given a sufficiently dense genomewide marker map, all the genetic variance is expected to be explained by the markers, and all quantitative trait loci (QTL) are in linkage disequilibrium (LD) with at least one marker (Calus et al. 2008). This allows GS to predict genomewide estimates of breeding values (GW-EBV) without the need of phenotyping the selection candidates. A potential cost reduction of up to 90% can be achieved for a breeding program by GS (Schaeffer 2006), because only a moderate number of individuals are required to have both known marker genotypes and phenotypes. These individuals form a reference data set for the estimation of GW-EBV. The knowledge obtained from the reference data set can be applied to the calculation of GW-EBV for the selection candidates on the basis of their marker genotypes, with an accuracy that is found in the validation of the prediction (Goddard and Hayes 2007).For a successful application of GS, based on a reference data set, to a usually much larger population of selection candidates without phenotypic records, accuracy of the prediction is a key issue to consider (Goddard and Hayes 2009). Since GS was first proposed by Meuwissen et al. (2001), many research works using simulated data have been performed on this issue (Calus and Veerkamp 2007; Habier et al. 2007; Kolbehdari et al. 2007; Calus et al. 2008; Solberg et al. 2008). The recent availability of genomewide dense SNP marker maps has made GS with real data feasible. Studies of the accuracy of genomic predictions have emerged in some animal species, including mice (Lee et al. 2008; Legarra et al. 2008), chickens (Gonzalez-Recio et al. 2009), and cattle (Hayes et al. 2009), and in plant species [for example, barley (Zhong et al. 2009)]. For GS applied to dairy cattle, accuracies for the GW-EBV have been reported in North American Holstein (VanRaden et al. 2009), Australian Holstein–Friesian (Hayes et al. 2009), and New Zealand Holstein–Friesian and Jersey dairy cattle (Harris et al. 2008).In the present work we applied GS to Norwegian Red dairy cattle to investigate the accuracy and possible bias of GW-EBV prediction for the phenotypes of milk production, clinical mastitis, and calving ease, by using real bovine genotyping data. Three methods, best linear unbiased prediction, Bayesian statistics, and a mixture model approach were used in the study, and their accuracies and biases of the GW-EBV were compared. To estimate the accuracy and bias of the GW-EBV the approach of cross-validation was employed, making use of estimates of breeding value from the Norwegian Red dairy cattle breeding scheme.     

Recording of the Reticular Motility in Cattle with Experimental and Spontaneous Traumatic Reticuloperitonitis

Using the method described in a previous publication for recording the reticulum action in normal cows the authors have investigated three cows with experimentally produced reticuloperitonitis as well as a group of cows with spontaneous peritonitis under treatment at the stationary clinic. The experimental animals were provided with permanent rumen fistulas. In the three cows with experimentally produced peritonitis the pointed object worked loose spontaneously during the first 24 hrs. The symptoms were mild after the first perforation and became aggravated after further perforations. An abnormally prolonged reticulum interval was recorded immediately after the perforation and continued to be clearly distinguishable for two to three days. In the cows with spontaneous peritonitis, this change was observed only in the acute stage. Prolongation of the second phase of the reticulum was the most pronounced finding from the recordings of the reticular movements, both in the cows with experimental peritonitis and in those in the spontaneous group. The change was at its height after two to seven days, and then subsided gradually in the experimental animals in which repeated recordings had been made. The pressure elevation in reticulum during the contractions was lower both in the animals with experimental peritonitis and in the spontaneous group than in the healthy animals. The cows with reticuloperitonitis also displayed impaired ability to pass the recording catheter from the atrium ruminis to the reticulum.     

Description of the Infection Status in a Norwegian Cattle Herd Naturally Infected by Mycobacterium avium subsp. paratuberculosis

The Norwegian surveillance and control programme for paratuberculosis revealed 8 seroreactors in a single dairy cattle herd that had no clinical signs of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) infection. Paratuberculosis had been a clinical problem in goats several years previously in this herd. All 45 cattle were culled and a thorough investigation of the infection status was conducted by the use of interferon-γ (IFN-γ) immunoassay, measurement of antibodies, and pathological and bacteriological examination.     

Methods of Recording Aphid Populations for Use in Research on Potato Virus Diseases

Methods of counting aphids infesting potato crops are reviewed and discussed. The results of an aphid survey in different parts of England during 1946 and of counts at Rothamsted during 1947 are given and used to illustrate methods of expressing the counts; the extent of virus spread in 1946 showed no close correlation with aphid numbers. It is concluded that an estimate of the number of aphids per plant is better for virus disease research than the number per 100 leaves. A method of estimating the number of aphids per plant with tolerable accuracy is described, but it is concluded that for most surveys a method which does not necessitate the counting of the aphids is adequate. Such a method, based on the percentage of leaves infested, is described.     

Plants Used for Reproductive Health by Nahua Women in Northern Veracruz,Mexico

Plants Used for Reproductive Health by Nahua Women in Northern Veracruz, Mexico. This paper reports the use of medicinal plants by Nahua women in the state of Veracruz, Mexico. It documents the women’s plant knowledge for reproductive purposes, which includes uses such as conception, pregnancy, birth, contraception, menstruation, post-partum, and general reproductive health. The concept of equilibrium is very important in regaining health among the Nahua; consequently, many of the medicinal plants have this as their primary purpose. The introduction of biomedical clinics and hospitals in the region has had a significant effect on the loss of knowledge about medicinal plants. Additionally, the midwives are not taking any new apprentices and laywomen are not passing on their knowledge to future generations. This generational gap contributes to the loss of knowledge about medicinal plants. This research contributes to the study of indigenous ethnobotany by (a) creating a record of the plant knowledge possessed by indigenous women, (b) giving voice to some of their health concerns, (c) indicating how the introduction of biomedicine has affected their plant use, and (d) providing a framework for understanding how marginal peoples around the world respond to the impact that globalization and change has on their health needs and local ethnobotanical knowledge.     

Evaluating the Quality of Cardiopulmonary Resuscitation in the Emergency Department by Real-Time Video Recording System

Objectives

To compare cardiopulmonary resuscitation (CPR) quality between manual CPR and miniaturized chest compressor (MCC) CPR. To improve CPR quality through evaluating the quality of our clinical work of resuscitation by real-time video recording system.

Methods

The study was a retrospective observational study of adult patients who experienced CPR at the emergency department of Shanghai Tenth People’s Hospital from March 2013 to August 2014. All the performance of CPR were checked back by the record of “digital real-time video recording system”. Average chest compression rate, actual chest compression rate, the percentage of hands-off period, time lag from patient arrival to chest compression, time lag from patient arrival to manual ventilation, time lag from patient arrival to first IV establish were compared. Causes of chest compression hands-off time were also studied.

Results

112 cases of resuscitation attempts were obtained. Average chest compression rate was over 100 compression per minute (cpm) in the majority of cases. However, indicators such as percentage of hands-off periods, time lag from patient arrival to the first manual ventilation and time lag from patient arrival to the first IV establish seemed to be worse in the manual CPR group compared to MCC CPR group. The saving of operators change time seemed to counteract the time spent on MCC equipment. Indicators such as percentage of hands-off periods, time lag between patient arrival to the first chest compression, time lag between patient arrival to the first manual ventilation and time lag from patient arrival to the first IV establish may influence the survival.

Conclusion

Our CPR quality remained to be improved. MCC may have a potentially positive role in CPR.     

Lymphocyte Stimulation and Leucocyte Migration Inhibition as Diagnostic Tools for the Detection of Mycobacterium Avium Infection in Cattle

The tuberculin skin test is the conventional method of detecting infections with mycobacteria in animals. A positive reaction is considered to reflect cell-mediated immunity (CMI). CMI against mycobacteria can be studied by in vitro systems using suspensions of blood lymphocytes or leucocytes. The reactivity of these cells to different antigens can be measured in the lymphocyte stimulation (LS) (Muscoplat et al 1975, Bergman 1976, Johnson & Morein 1976), or leucocyte migration inhibition (LMI) (Aalund 1970, Clausen 1973) tests.     

Development of an Intelligent Mobile Health Monitoring System for the Health Surveillance System in Indonesia

Mobile phone applications have been widely used in various fields, including health care. Generally, this technology is used to overcome problems in health care by utilising mobile phone features for facilitating basic needs in health services. This study proposes an intelligent mobile health monitoring system that can be used in rural and remote areas where health services are still lacking. The system was made based on client/server architecture. Nine symptoms of typhoid, cough and diarrhoea from 30 patients were gathered from a hospital. Based on this data, a machine learning model using Support Vector Machine (SVM) was performed to distinguish these diseases. To find the best model parameters of the SVM, three different kernels (linear, polynomial, and Radial Basis Function (RBF)) were analysed. The result showed that RBF with degree 2 provided the best result in this particular application. The system was designed to receive input from patients about symptoms of the disease they have. The mobile phone application sends the data of the symptoms using Short Message Service (SMS) to the server. Furthermore, a machine algorithm module in the server identifies to which disease it belongs to based on the machine learning model created before. The prediction result is accessible to the doctor and the nearest Community Health Center (CHC). Based on the result, the doctor proposes a treatment plan for the patient to be recorded and sent to the patient by CHC. The proposed mobile health monitoring system has run properly and is ready to be evaluated in a real situation.     

A Computer-Controlled Video System for Real-Time Recording of Insect Flight in Three Dimensions

A computer-controlled video system for real-time recording of insect flight in three dimensions is described. The flight paths of moths were recorded in a flight tunnel using two CCD cameras placed adjacent to each other at angles of 45 and 135° to the flight tunnel axis and separated by a distance of 120 cm. They were connected to two 2⁸-level gray-scale frame grabbers via two external synchronizers. The two-dimensional coordinates of the flying insect were obtained from the two cameras at 40-ms intervals and transferred to host computer for processing and monitor for real-time display. Due to speed limitation in the image acquisition hardware, construction of the three-dimensional file was carried off-line. The flying insect was rendered as a dark spot in a bright background using a homogeneous light source. As the insect enters into the field of view of the two cameras, the light distribution changes, and the frame grabber detects only those variation in the light distribution which results from a flying insect. The target insect can be as small as 3 pixels and can be tracked in a stereoscopic field of view 60 cm long and 50 cm high. A method was developed that allowed for scalar scoring of various pheromone sources to assess their attractiveness using vector flight parameters. This method was applied successfully for optimization of pheromone blend of the grapevine moth, Lobesia botrana.     

Genetic Variants in Diseases of the Extrapyramidal System

Knowledge on the genetics of movement disorders has advanced significantly in recent years. It is now recognized that disorders of the basal ganglia have genetic basis and it is suggested that molecular genetic data will provide clues to the pathophysiology of normal and abnormal motor control. Progress in molecular genetic studies, leading to the detection of genetic mutations and loci, has contributed to the understanding of mechanisms of neurodegeneration and has helped clarify the pathogenesis of some neurodegenerative diseases. Molecular studies have also found application in the diagnosis of neurodegenerative diseases, increasing the range of genetic counseling and enabling a more accurate diagno-sis. It seems that understanding pathogenic processes and the significant role of genetics has led to many experiments that may in the future will result in more effective treatment of such diseases as Parkinson’s or Huntington’s. Currently used molecular diagnostics based on DNA analysis can identify 9 neurodegenerative diseases, including spinal cerebellar ataxia inherited in an autosomal dominant manner, dentate-rubro-pallido-luysian atrophy, Friedreich’s disease, ataxia with ocu-lomotorapraxia, Huntington''s disease, dystonia type 1, Wilson’s disease, and some cases of Parkinson''s disease.     

New Diagnostic System for the Identification of Lactose-fermenting Gram-negative Rods

The identification of prompt lactose-fermenting gram-negative rods has generally relied heavily upon colonial morphology coupled with one or more indole, methyl red, Voges-Proskauer, citrate (IMViC) parameters, hydrogen sulfide, and motility. Studies were undertaken to compare diagnoses dependent solely upon the more orthodox criteria to a system for identification based upon hydrogen sulfide, ornithine decarboxylase, and citrate utilization (HOC). The results suggest that the IMViC scheme of identification is neither consistent nor applicable when applied to the current nomenclature of the above group of organisms and should be discarded, whereas the HOC system may prove to be of significant value to clinical microbiologists.     

Association of Veterinary Third-Generation Cephalosporin Use with the Risk of Emergence of Extended-Spectrum-Cephalosporin Resistance in Escherichia coli from Dairy Cattle in Japan

The use of extended-spectrum cephalosporins in food animals has been suggested to increase the risk of spread of Enterobacteriaceae carrying extended-spectrum β-lactamases to humans. However, evidence that selection of extended-spectrum cephalosporin–resistant bacteria owing to the actual veterinary use of these drugs according to criteria established in cattle has not been demonstrated. In this study, we investigated the natural occurrence of cephalosporin-resistant Escherichia coli in dairy cattle following clinical application of ceftiofur. E. coli isolates were obtained from rectal samples of treated and untreated cattle (n = 20/group) cultured on deoxycholate-hydrogen sulfide-lactose agar in the presence or absence of ceftiofur. Eleven cefazoline-resistant isolates were obtained from two of the ceftiofur-treated cattle; no cefazoline-resistant isolates were found in untreated cattle. The cefazoline-resistant isolates had mutations in the chromosomal ampC promoter region and remained susceptible to ceftiofur. Eighteen extended-spectrum cephalosporin–resistant isolates from two ceftiofur-treated cows were obtained on ceftiofur-supplemented agar; no extended-spectrum cephalosporin–resistant isolates were obtained from untreated cattle. These extended-spectrum cephalosporin–resistant isolates possessed plasmid-mediated β-lactamase genes, including bla _CTX-M-2 (9 isolates), bla _CTX-M-14 (8 isolates), or bla _CMY-2 (1 isolate); isolates possessing bla _CTX-M-2 and bla _CTX-M-14 were clonally related. These genes were located on self-transmissible plasmids. Our results suggest that appropriate veterinary use of ceftiofur did not trigger growth extended-spectrum cephalosporin–resistant E. coli in the bovine rectal flora; however, ceftiofur selection in vitro suggested that additional ceftiofur exposure enhanced selection for specific extended-spectrum cephalosporin–resistant β-lactamase-expressing E. coli clones