Surgical Decompression in Dogs with Thoracolumbar Intervertebral Disc Disease and Loss of Deep Pain Perception: A Retrospective Study of 46 Cases

The case details and outcome after surgical decompression of 46 dogs with thoracolumbar intervertebral disc disease with loss of deep pain perception prior to surgery were reviewed. Nineteen dogs (41.3%) recovered with a median follow-up period of 12.5 months. Recovery was defined as an ambulatory paraparesis, or better, with urinary and fecal continence. There was a better outcome in dogs with loss of deep pain for less than 24 hours prior to surgery (19/41; 46.3% recovered) than in dogs without deep pain perception for more than 24 hours (0/5; 0% recovered). Dogs with deep pain perception present at two weeks postoperatively had significantly higher success rate (8/12; 66.7% recovered) than dogs without deep pain perception at this time period (1/10; 10.0% recovered). The return of deep pain perception by two weeks postoperatively can be a useful positive prognostic indicator.     

Calcification of Intervertebral Discs in the Dachshund: A Radiographic Study of 115 Dogs at 1 and 5 Years of Age

The vertebral columns of 115 dachshunds were x-rayed at 1 and 5 years of age. This sample represented 5.7% of all dachshunds registered with the Norwegian Kennel Club in the period 1986-1988. All dogs were clinically normal at the commencement of the study. At 1 year of age calcified intervertebral discs were identified in 34 (29.6%) of the dogs and the number of calcified discs in each individual varied from 1 to 7 with a mean of 2.7. At 5 years of age calcified discs were identified in 66 (57.4%) of the dogs and the number of calcified discs in each individual varied from 1 to 11 with a mean of 3.2. Of all dogs in which calcified discs were identified at 1 year of age, 33 (97.1%) were found to have calcified discs also at 5 years of age. Of 92 calcified discs identified in the dogs at 1 year of age, 29 (31.5%) were not calcified 4 years later. Of 211 calcified discs identified in the dogs at 5 years of age, 148 (70.1%) were not calcified 4 years before. From 1 to 5 years of age, signs of spinal disease were registered in 12 (35.3%) of the dogs in which calcified discs were identified at 1 year of age, and in 7 (8.6%) of the dogs in which calcified discs were not identified at 1 year of age. Of all dogs in which one or more calcified discs had disappeared during the study-period, signs of spinal disease were registered in 9 (75.0%).     

Molecular Investigations of Rickettsia helvetica Infection in Dogs,Foxes, Humans,and Ixodes Ticks

Rickettsia helvetica, a tick-borne member of the spotted-fever-group rickettsiae, is a suspected pathogen in humans; however, its role in animals is unknown. The aims of this study were to establish a R. helvetica-specific real-time TaqMan PCR assay and apply it to the analysis of tick vectors (to determine potential exposure risk) and blood samples from Canidae and humans (to determine prevalence of infection). The newly designed 23S rRNA gene assay for R. helvetica was more sensitive than a published citrate synthase gene (gltA) assay for several rickettsiae. Blood samples from 884 dogs, 58 foxes, and 214 human patients and 2,073 ticks (Ixodes spp.) collected from either vegetation or animals were analyzed. Although the maximal likelihood estimate of prevalence was 12% in unfed ticks and 36% in ticks collected from animals, none of the 1,156 blood samples tested PCR positive. Ticks from cats were more frequently PCR positive than ticks from dogs. Sequencing of the 23S rRNA and/or the gltA gene of 17 tick pools confirmed the presence of R. helvetica. Additionally, Rickettsia monacensis, which has not been previously found in Switzerland, was identified. In conclusion, R. helvetica was frequently detected in the tick population but not in blood samples. Nevertheless, due to the broad host range of Ixodes ticks and the high rate of infestation with this agent (i.e., R. helvetica was 13 times more frequent in unfed ticks than the tick-borne encephalitis virus), many mammals may be exposed to R. helvetica. The PCR assay described here represents an important tool for studying this topic.Tick-borne rickettsioses are caused by intracellular bacteria belonging to the spotted fever group (SFG) of the genus Rickettsia. The latter comprises more than 20 different species, of which an increasing number are known to be associated with human and animal diseases. The SFG rickettsiae are distributed worldwide, and their distribution depends upon the occurrence of tick species. The most common tick in Europe is Ixodes ricinus, which was found to harbor Rickettsia helvetica. R. helvetica is transmitted not only transstadially but also transovarially in I. ricinus. Therefore, this tick is both a vector and a reservoir for R. helvetica. Due to the broad host range of I. ricinus, many mammalian species, including humans, can serve as hosts. Therefore, these host species may potentially be exposed to R. helvetica. R. helvetica is a suspected pathogen in humans, and the symptoms described for infections in humans include fever, headache, arthralgia, and myalgia (1, 3, 7, 21, 34). The agent also has been implicated in two cases of fatal perimyocarditis (20, 22).Interestingly, despite the wide distribution of I. ricinus ticks and the high rate of infection of these ticks with R. helvetica that has been reported in several European countries (2, 9, 18, 19, 25, 29, 35, 42), larger studies discussing the prevalence of the infection in humans and animals are scarce. No studies evaluating the importance of R. helvetica in pets or farm animals are available as yet. It is unknown whether these animals can serve as a reservoir or develop clinical signs after infection.Rickettsial infections have been reported to represent the third most common vector-borne disease acquired during international travel and are therefore considered a common cause of fever of unknown origin in returned travelers (24). As the occurrence of tick-borne infectious diseases, and particularly rickettsial infections, is increasing in humans worldwide (26), it may be assumed that the same holds true for companion animals. In dogs, fever of unknown origin that is responsive to antibiotic treatment is frequently observed. In these cases, an infectious agent is suspected but rarely, if ever, confirmed. R. helvetica infections may be the underlying cause in some of these cases, even if the patient does not have a travel history, since exposure to R. helvetica-infected I. ricinus ticks may have occurred locally.To date, the diagnosis of rickettsial infection has most often been confirmed by serological testing. However, antibodies are not detectable prior to the second week of illness for any rickettsial disease studied thus far. Moreover, except for detection of seroconversion or a fourfold increase in titer, a positive serology result does not necessarily indicate an acute infection. A standardized sensitive and specific molecular method for the confirmation of R. helvetica infections would facilitate not only its diagnosis but also prevalence studies. This in turn could increase the awareness of physicians and veterinarians who are confronted with diseased individuals.Therefore, the aims of the present study were as follows: first, to establish a sensitive real-time PCR assay specific for R. helvetica; second, to study tick vectors for R. helvetica to assess the potential exposure risk for animals and humans; and third, to evaluate blood samples from Canidae and humans to assess the occurrence of R. helvetica infections.(These studies were conducted by A. Perreten as partial fulfillment of the requirements for a doctoral thesis at the Vetsuisse Faculty, University of Zurich.)     

Effects of Humic Acid on Protein Synthesis and Ion Uptake in Beet Discs

Humic acids stimulate the development of phosphate uptake-capacityin beet discs during ageing under aseptic conditions withoutaffecting phosphate uptake per se. Chloride uptake is inhibitedby humic acids as is the development of chloride uptake capacity.The uptake of proline and leuoine is not influenced by ageingin humic acid. While measurements of the incorporation of theseamino acids into the sub-cellular particles of discs indicatethat humic acid does not affect protein synthesis in general,the stimulation of invertase development during ageing doesshow a definite effect on some aspect of protein synthesis.     

Effects of pH and Ethephon on Betacyanin Leakage from Beet Root Discs

Betacyanin leakage from beet root discs was found to increase with decreasing pH of the incubation medium. Although 10 millimolar Ca²⁺ reduced pigment leakage at pH 3.5, it was ineffective at pH 2.3. Leakage was also stimulated by 100 micrograms per milliliter (2-chloroethyl)-phosphonic acid (pH 3.1), but when the solution was neutralized, this leakage was eliminated. Bubbling C₂H₄ through a neutral medium containing beet discs had no effect on pigment leakage; it appears that the effect of Ethephon solutions on this process is a function of their low pH.     

Procedure for the Transfer of Small Discs of Agar-Containing Media Under Sterile Conditions and Some Applications of This Technique (Agar Disc Auxanography)

Small agar discs are formed and transferred under sterile conditions. This technique permits the efficient and versatile use of the petri plate when many growth conditions and (or) cultures are being examined.     

Effects of Gastric Freezing on the Isolated Denervated Gastric Pouch in Dogs

The purpose of this study was to investigate the effects of gastric freezing on the chief and parietal cells of the stomach. To carry this out six Heidenhain pouch dogs were prepared. The secretions of these pouches were studied, both for HCl levels and pepsin activity, for 30 days before and 30 days after the freezing of the pouch. Freezing was carried out for one hour at -17 to -20° C. The results show that no essential depression of either the HCl levels or the pepsin activity of the juice was effected by freezing. Therefore, it is deduced that freezing does not affect the functional activity of either the chief or the parietal cells. Complications such as ulceration and fistula formation into adjacent organs occurred as a result of freezing in certain pouch dogs.     

Effects of Vasopressin on Blood-Brain Transfer of Methionine in Dogs

We used a simplified probe detection system for positron-emitting radionuclides in order to measure blood-brain barrier transport of amino acids in anesthetized dogs. Plasma and brain time-activity curves were recorded after intravenous bolus injection of L-[11C]methionine before and after administration of 1 microgram of vasopressin. Three-compartment models with three or four transfer coefficients were used to derive the kinetics of L-[11C]methionine uptake in brain. The blood-brain clearance of the tracer (K1) was 0.075 ml ml-1 min-1 before and 0.041 ml ml-1 min-1 after injection of vasopressin. The partition volume and the initial distribution (plasma) volume of methionine were unchanged and within the expected limits. The net accumulation rate of methionine (K), estimated by both the four-parameter (kinetic) and three-parameter (graphic) approaches, decreased after vasopressin injection in all six studies.     

Dogs in child psychotherapy: Effects on state of mind

Abstract

Using a pretest-posttest design, this study investigates possible influences of animal-assisted therapy (AAT), using a dog, on the state of mind of children and adolescents who have undergone inpatient psychiatric treatment. To measure this, the Basler Befindlichkeits-Skala (BBS) was used, which measures general “state of mind” and provides four sub-scale scores: vitality, intra-emotional balance, social extroversion, and alertness. For Group 1 patients (n = 61, with AAT), the results show highly significant increases in all dimensions of the BBS. These changes were not found in a second group (Group 2, n = 39), in which there was no AAT. There was a significant negative correlation between pretest BBS scores and the change in scores that occurred after therapy incorporating AAT. Among seven patients in Group 1, a deterioration in state of mind was recorded. Under our controlled clinical conditions, an effect size of 0.38 was calculated for the therapy using a dog. Incorporating a dog could catalyze psychotherapeutic work with children and adolescents.     

Effects of Group and Individual Housing on the Behavior of Kennelled Dogs in Animal Shelters

Abstract

Recent findings have led researchers to believe that our pets may do more for us than we think. A dog's presence may even make a person appear more likable. Two studies were conducted to test this hypothesis. In the first study, 34 subjects viewed photographs of people pictured alone, pictured with a dog, and pictured with flowers. The subjects were asked to rate the person in the photos with regard to four dimensions: approachability, happy looking, relaxed looking, and best photo. The central finding revealed that photos of individuals pictured with a dog were rated higher than photos of individuals pictured without a dog. In the second study 45 subjects were asked to view three types of slides. The three slides consisted of outdoor scenery, a person walking through the outdoor scene, and a person walking a dog through the outdoor scene. Subjects were asked to rate the slides against each other with regard to several aesthetic dimensions, which included easiest to gaze at and best photo. In addition, subjects were asked how they perceived the person photographed with respect to happiness, relaxed looking, and safety. Finally, subjects were asked to choose photos in which they would like to be included. The major results of Study II demonstrated that (1) photos rated highest for questions concerning subjects' feelings toward the slides (easy to gaze at, feeling relaxed, best photo) were the scenery slides shown alone. The second highest rating went to the slides of the person walking the dog. (2) The person shown in the scene was perceived as appearing happier and safer when with the dog. (3) Subjects preferred to be added to the scene shown alone, without the dog. And when given the choice to replace the person shown, subjects preferred to replace the person in the scene with the dog rather than the person shown alone. Overall, results of both Studies I and II indicate that people appear happier, safer, and make a better “picture” when seen with a dog. Also, the results show that people appear more relaxed when sitting or standing with a dog. Finally, when asked to place themselves in the scene, subjects would rather be in a scene with a dog than alone.     

A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches

Painful degenerative disc diseases have been targeted by different biological treatment approaches. Nucleus pulposus (NP) cells play a central role in intervertebral disc (IVD) maintenance by orchestrating catabolic, anabolic and inflammatory factors that affect the extracellular matrix. IVD degeneration is associated with imbalances of these factors, resulting in a catabolic inflammatory metabolism. Therefore, accurate knowledge about their quantity and quality with regard to matrix synthesis is vital for a rational gene therapeutic approach. NP cells were isolated from 63 patients operated due to lumbar disc herniation (mean age 56 / range 29 - 84 years). Then, three-dimensional culture with low-glucose was completed in a collagen type I scaffold for four weeks. Subsequently cell proliferation evaluation was performed using 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide and intracellular concentration of 28 endogenously expressed anabolic, catabolic, inflammatory factors and relevant matrix proteins was determined by enzyme-linked immunosorbent assay. Specimen-related grades of degeneration were confirmed by preoperative magnetic resonance imaging. Independent from gender, age and grade of degeneration proliferation rates remained similar in all groups of NP cells. Progressive grades of degeneration, however, showed a significant influence on accumulation of selective groups of factors such as disintegrin and metalloproteinase with thrombospondin motifs 4 and 5, matrix metalloproteinase 3, metalloproteinase inhibitor 1 and 2, interleukin-1β and interleukin-1 receptor. Along with these changes, the key NP matrix proteins aggrecan and collagen II decreased significantly. The concentration of anabolic factors bone morphogenetic proteins 2, 4, 6 and 7, insulin-like growth factor 1, transforming growth factor beta 1 and 3, however, remained below the minimal detectable quantities. These findings indicate that progressive degenerative changes in NP may be problematic with regard to biologic treatment strategies. Hence, gene therapeutic interventions regulating relevant bioactive factors identified in this work might contribute to the development of regenerative treatment approaches for degenerative disc diseases.     

Conservative and Surgical Aspects of Disc Lesion Management: Follow-Up Review of 244 Cases

A follow-up study of 244 patients who were admitted to hospital with the diagnosis of discogenic disease between 1963 and 1970 was conducted to determine more precisely when conservative care should stop and a myelogram study followed by surgical decompression should be considered.The number of patients who had electromyography was separately tabulated and totaled (154). Of these, 87 (56.5 percent) had normal and 67 (43.5 percent) abnormal findings. It is significant that 29 of the total had positive myelograms and operation, yet 16 (59 percent) of the 29 had normal electromyograms. This raises a question about how much confidence to place in negative electromyographic findings when deciding for or against surgical operation. However, negative electromyograms are very helpful in reassuring patients who are emotional and resistant to resuming useful activities.     

Effects of Phenylbutazone and Indomethacin on the Post-operative Course following Experimental Orthopaedic Surgery in Dogs

Randomized placebo-controlled crossover studies were carried out in dogs to evaluate how two non-steroidal antiinflammatory drugs (NSAID) might modulate an acute post-traumatic inflammatory reaction. Two “identical” surgical interventions were performed on the forelimbs of each animal with an interval of 28 days, to enable a paired comparison of the inflammatory signs and the wound/bone healing processes. At one operation 8 dogs received 300 mg phenylbutazone twice daily for 8 days starting on the day before surgery, and at the other operation matching placebo tablets were given. In a similar placebo-controlled trial another group of 8 dogs received 5 mg indomethacin twice daily. With phenylbutazone the post-operative swelling was not significantly reduced compared to placebo, but there was less pain and limping. With indomethacin the swelling was somewhat reduced, but there was no consistent difference to placebo in the pain and limping assessments. None of the drugs appeared to distinctly effect the wound or fracture healing, as evaluated by clinical inspection, comparison of radiographs and comparison of bone sections from the sites of surgery. It proved difficult to select an appropriate dosage of indomethacin due to its high potential to induce GI ulceration and bleeding in dogs. In this experimental surgical model with an acute inflammation, neither phenylbutazone nor indomethacin showed impressive anti-inflammatory or analgesic properties. In the same model paracetamol has proved to significantly and more efficiently, reduce both swelling and pain without any noticeable adverse effects, and appears to be a better alternative than the two presently tested NSAID.     

980 nm半导体激光照射对种植体表面结构和温度影响的实验研究

目的:980 nm半导体激光可用于种植体周围软组织处理和种植体周围炎治疗,但其对种植体的作用尚未完全被了解。本文研究980 nm半导体激光照射对种植体表面结构和温度变化的影响,以期为临床使用参数设置和操作方法提供依据。方法:980 nm半导体激光照射纯钛圆盘试件,扫描电镜观察钛盘表面结构变化,热电偶检测钛盘温度变化范围和到达骨组织损伤温度升高阈值(10℃)的照射时长。结果:实验全部参数设置下980 nm半导体激光照射,钛盘表面结构均无明显改变。脉冲模式输出功率1 W,移动照射20 s钛盘温度上升19.8℃;经过10.2 s钛盘温度升高可达10℃。连续输出模式或增加输出功率,钛盘温度显著上升,并可在数秒内超过10℃。结论:980 nm半导体激光照射对种植体表面结构无损伤,但存在种植体温度升高造成周围组织热损伤的风险。     

丙泊酚复合麻醉在实验犬外科手术中的效果观察

目的丙泊酚复合麻醉应用于实验犬外科手术,进行效果评价。方法成年健康杂种犬13只,雌雄不限。术前30 min肌内注射阿托品0.5 mg,吗啡10 mg,进行气管插管,静脉注射氯胺酮50 mg,静脉注射丙泊酚首次剂量5 mg/kg体重,维持剂量1 mg/kg。结果丙泊酚复合麻醉,平均麻醉起效时间40 s,首次剂量平均维持17.3min,重复给药平均维持13.6 min,无死亡。丙泊酚有较强的麻醉效果,诱导时间短,起效快,恢复快速平稳,而且无副作用。结论丙泊酚复合麻醉适合于犬的外科手术实验,是一种较为理想的麻醉方法。     

Neutering Dogs: Effects on Joint Disorders and Cancers in Golden Retrievers

In contrast to European countries, the overwhelming majority of dogs in the U.S. are neutered (including spaying), usually done before one year of age. Given the importance of gonadal hormones in growth and development, this cultural contrast invites an analysis of the multiple organ systems that may be adversely affected by neutering. Using a single breed-specific dataset, the objective was to examine the variables of gender and age at the time of neutering versus leaving dogs gonadally intact, on all diseases occurring with sufficient frequency for statistical analyses. Given its popularity and vulnerability to various cancers and joint disorders, the Golden Retriever was chosen for this study. Veterinary hospital records of 759 client-owned, intact and neutered female and male dogs, 1–8 years old, were examined for diagnoses of hip dysplasia (HD), cranial cruciate ligament tear (CCL), lymphosarcoma (LSA), hemangiosarcoma (HSA), and mast cell tumor (MCT). Patients were classified as intact, or neutered early (<12 mo) or late (≥12 mo). Statistical analyses involved survival analyses and incidence rate comparisons. Outcomes at the 5 percent level of significance are reported. Of early-neutered males, 10 percent were diagnosed with HD, double the occurrence in intact males. There were no cases of CCL diagnosed in intact males or females, but in early-neutered males and females the occurrences were 5 percent and 8 percent, respectively. Almost 10 percent of early-neutered males were diagnosed with LSA, 3 times more than intact males. The percentage of HSA cases in late-neutered females (about 8 percent) was 4 times more than intact and early-neutered females. There were no cases of MCT in intact females, but the occurrence was nearly 6 percent in late-neutered females. The results have health implications for Golden Retriever companion and service dogs, and for oncologists using dogs as models of cancers that occur in humans.