共查询到20条相似文献,搜索用时 46 毫秒
1.
Saika H Okamoto M Miyoshi K Kushiro T Shinoda S Jikumaru Y Fujimoto M Arikawa T Takahashi H Ando M Arimura S Miyao A Hirochika H Kamiya Y Tsutsumi N Nambara E Nakazono M 《Plant & cell physiology》2007,48(2):287-298
A rapid decrease of the plant hormone ABA under submergence is thought to be a prerequisite for the enhanced elongation of submerged shoots of rice (Oryza sativa L.). Here, we report that the level of phaseic acid (PA), an oxidized form of ABA, increased with decreasing ABA level during submergence. The oxidation of ABA to PA is catalyzed by ABA 8'-hydroxylase, which is possibly encoded by three genes (OsABA8ox1, -2 and -3) in rice. The ABA 8'-hydroxylase activity was confirmed in microsomes from yeast expressing OsABA8ox1. OsABA8ox1-green fluorescent protein (GFP) fusion protein in onion cells was localized to the endoplasmic reticulum. The mRNA level of OsABA8ox1, but not the mRNA levels of other OsABA8ox genes, increased dramatically within 1 h after submergence. On the other hand, the mRNA levels of genes involved in ABA biosynthesis (OsZEP and OsNCEDs) decreased after 1-2 h of submergence. Treatment of aerobic seedlings with ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC), rapidly induced the expression of OsABA8ox1, but the ethylene treatment did not strongly affect the expression of ABA biosynthetic genes. Moreover, pre-treatment with 1-methylcyclopropene (1-MCP), a potent inhibitor of ethylene action, partially suppressed induction of OsABA8ox1 expression under submergence. The ABA level was found to be negatively correlated with OsABA8ox1 expression under ACC or 1-MCP treatment. Together, these results indicate that the rapid decrease in ABA levels in submerged rice shoots is controlled partly by ethylene-induced expression of OsABA8ox1 and partly by ethylene-independent suppression of genes involved in the biosynthesis of ABA. 相似文献
2.
《Journal of Asia》2023,26(2):102014
The incidence of drought stress in plants has been increasing due to global warming, and the phytohormone abscisic acid (ABA) induces the formation of physical barriers in plants, such as callose accumulation. The brown planthopper (BPH; Nilaparvata lugens Stål) occurs throughout Asia and feeds on rice, but the effects of drought stress on BPH feeding remain unclear. In this study, we observed changes in callose formation and ABA content in rice during drought stress. ABA content and the relative expression of ABA synthesis genes OsNCED3 and OsNCED5 were higher in drought-stressed rice than the non-stressed control. Similarly, the expression levels of callose synthesis genes and callose deposition were significantly higher in drought-stressed rice as compared to non-stressed plants, and this impacted BPH feeding. Our results indicated that rice resistance to BPH increased during drought stress due to the accumulation of callose and increasing ABA levels. Our findings provide a basis for understanding BPH feeding performance on rice during drought stress and offer novel insights relative to control during periods of water shortage. 相似文献
3.
4.
Arabidopsis F-box gene FOA1 involved in ABA signaling 总被引:1,自引:0,他引:1
Juan Peng DaShi Yu LiQun Wang MinMin Xie CongYing Yuan Yu Wang DongYing Tang XiaoYing Zhao XuanMing Liu 《中国科学:生命科学英文版》2012,55(6):497-506
5.
Wu Yang Tian Fan Xiaoying Hu Taihui Cheng Mingyong Zhang 《Journal of Plant Biology》2017,60(5):485-492
The miRNA171 family is one of the well-conserved miRNA families, and its role under stresses is not known except its expression on genome-wide expression analyses. osa-miR171c was induced by high concentration of salt (150 mM NaCl). A rice dh mutant with osa-miR171c overexpression triggered by a T-DNA insertion, significantly decreased salt tolerance at the stages of germination and seedling. This phenotype was confirmed by osa-miR171c overexpression transgenic rice. Compared with wild-type (WT), dh mutant reduced amounts of free proline and increased the water loss rate after salt treatment. Stomatal density in the leaf epidermis of dh mutant also increased. Moreover, dh mutant increased sensitivity to ABA treatment. Several stressresponsive genes were down-regulated in dh mutant than in WT under salty stress. These results indicate that osamiR171c is involved in modulating physiological changes, stomatal development, ABA-dependent pathways and expression of stress-related genes; thereby, it possibly contributes to salty tolerance. 相似文献
6.
Dongsu Choi 《Journal of Plant Biology》2011,54(6):365-372
Recent studies revealed that some rice varieties adopt opposite strategies to overcome flooding stress. While certain varieties
hold metabolism and stay stunted until floodwater recedes, deepwater rice varieties undergo rapid stem elongation and do not
suffer drowning problems. Both varieties use the same signaling agents, the ethylene response factors, as key factors even
though they display opposite submergence responses. In deepwater rice, ethylene response factor genes SNORKEL1 and SNORKEL2 are believed to play a major role in submergence escape by mediating ethylene signaling, which leads to rapid stem elongation.
These genes connect hormone signaling cascades from ethylene to ABA and gibberellins (GAs). Submergence increases ethylene
levels in the internodal space, ethylene upregulates an ABA inactivating enzyme gene, OsCYP707A5 or OsABA8ox1, and some GA metabolism genes such as OsGA20ox genes and OsGA3ox genes. As a result of gene regulation by ethylene, internodal ABA levels decrease while GA levels increase, finally upregulating
growth-related genes like expansin genes (OsEXPs). Along with the ethylene signaling in submergence, it is necessary to consider an alternative signaling pathway induced
by hypoxia. Taken together, study on the submergence responses of rice plants will lead to improvement of crop production
and contribution to basic research on plant growth. 相似文献
7.
Silicon amendment to rice plants contributes to reduced feeding in a phloem‐sucking insect through modulation of callose deposition
下载免费PDF全文
![点击此处可从《Ecology and evolution》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Silicon (Si) uptake by Poaceae plants has beneficial effects on herbivore defense. Increased plant physical barrier and altered herbivorous feeding behaviors are documented to reduce herbivorous arthropod feeding and contribute to enhanced plant defense. Here, we show that Si amendment to rice (Oryza sativa) plants contributes to reduced feeding in a phloem feeder, the brown planthopper (Nilaparvata lugens, BPH), through modulation of callose deposition. We associated the temporal dynamics of BPH feeding with callose deposition on sieve plates and further with callose synthase and hydrolase gene expression in plants amended with Si. Biological assays revealed that BPH feeding was lower in Si‐amended than in nonamended plants in the early stages post‐BPH infestation. Histological observation showed that BPH infestation triggered fast and strong callose deposition in Si‐amended plants compared with nonamended plants. Analysis using qRT‐PCR revealed that expression of the callose synthase gene OsGSL1 was up‐regulated more and that the callose hydrolase (β‐1,3‐glucanase) gene Gns5 was up‐regulated less in Si‐amended than in nonamended plants during the initial stages of BPH infestation. These dynamic expression levels of OsGSL1 and Gns5 in response to BPH infestation correspond to callose deposition patterns in Si‐amended versus nonamended plants. It is demonstrated here that BPH infestation triggers differential gene expression associated with callose synthesis and hydrolysis in Si‐amended and nonamended rice plants, which allows callose to be deposited more on sieve tubes and sieve tube occlusions to be maintained more thus contributing to reduced BPH feeding on Si‐amended plants. 相似文献
8.
9.
García-Andrade J Ramírez V Flors V Vera P 《The Plant journal : for cell and molecular biology》2011,67(5):783-794
In the present study, we evaluated the role of the defense-related gene OCP3 in callose deposition as a response to two necrotrophic fungal pathogens, Botrytis cinerea and Plectosphaerella cucumerina. ocp3 plants exhibited accelerated and intensified callose deposition in response to fungal infection associated with enhanced disease resistance to the two pathogens. A series of double mutant analyses showed potentiation of callose deposition and the heightened disease resistance phenotype in ocp3 plants required the plant hormone abscisic acid (ABA) and the PMR4 gene encoding a callose synthase. This finding was congruent with an observation that ocp3 plants exhibited increased ABA accumulation, and ABA was rapidly synthesized following fungal infection in wild-type plants. Furthermore, we determined that potentiation of callose deposition in ocp3 plants, including enhanced disease resistance, also required jasmonic acid (JA) recognition though a COI1 receptor, however JA was not required for basal callose deposition following fungal infection. In addition, potentiation of callose deposition in ocp3 plants appeared to follow a different mechanism than that proposed for callose β-amino-butyric acid (BABA)-induced resistance and priming, because ocp3 plants responded to BABA-induced priming for callose deposition and induced resistance of a magnitude similar to that observed in wild-type plants. Our results point to a model in which OCP3 represents a specific control point for callose deposition regulated by JA yet ultimately requiring ABA. These results provide new insights into the mechanism of callose deposition regulation in response to pathogen attack; however the complexities of the processes remain poorly understood. 相似文献
10.
A rice gene activation/knockout mutant resource for high throughput functional genomics 总被引:9,自引:6,他引:3
Hsing YI Chern CG Fan MJ Lu PC Chen KT Lo SF Sun PK Ho SL Lee KW Wang YC Huang WL Ko SS Chen S Chen JL Chung CI Lin YC Hour AL Wang YW Chang YC Tsai MW Lin YS Chen YC Yen HM Li CP Wey CK Tseng CS Lai MH Huang SC Chen LJ Yu SM 《Plant molecular biology》2007,63(3):351-364
Using transfer DNA (T-DNA) with functions of gene trap and gene knockout and activation tagging, a mutant population containing
55,000 lines was generated. Approximately 81% of this population carries 1–2 T-DNA copies per line, and the retrotransposon
Tos17 was mostly inactive in this population during tissue culture. A total of 11,992 flanking sequence tags (FSTs) have been obtained
and assigned to the rice genome. T-DNA was preferentially (∼80%) integrated into genic regions. A total of 19,000 FSTs pooled
from this and another T-DNA tagged population were analyzed and compared with 18,000 FSTs from a Tos17 tagged population. There was difference in preference for integrations into genic, coding, and flanking regions, as well
as repetitive sequences and centromeric regions, between T-DNA and Tos17; however, T-DNA integration was more evenly distributed in the rice genome than Tos17. Our T-DNA contains an enhancer octamer next to the left border, expression of genes within genetics distances of 12.5 kb
was enhanced. For example, the normal height of a severe dwarf mutant, with its gibberellin 2-oxidase (GA2ox) gene being activated
by T-DNA, was restored upon GA treatment, indicating GA2ox was one of the key enzymes regulating the endogenous level of GA.
Our T-DNA also contains a promoterless GUS gene next to the right border. GUS activity screening facilitated identification
of genes responsive to various stresses and those regulated temporally and spatially in large scale with high frequency. Our
mutant population offers a highly valuable resource for high throughput rice functional analyses using both forward and reverse
genetic approaches.
Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users.
Yue-Ie Hsing, Chyr-Guan Chern, and Ming-Jen Fan have contributed equally. 相似文献
11.
Su Jin Jang Soo Jin Wi Yoo Jin Choi Gynheung An Ky Young Park 《Molecules and cells》2012,34(3):251-262
A highly oxidative stress-tolerant japonica rice line was isolated by T-DNA insertion mutation followed by screening in the presence of 50 mM H2O2. The T-DNA insertion was mapped to locus Os09g0547500, the gene product of which was annotated as lysine decarboxylase-like protein (GenBank accession No. AK062595). We termed this gene OsLDC-like 1, for Oryza sativa lysine decarboxylase-like 1. The insertion site was in the second exon and resulted in a 27 amino acid N-terminal deletion. Despite this defect in OsLDC-like 1, the mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress. The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining. Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenouslyapplied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves. Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H2O2. Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype. These results suggest that increased cellular PA levels have a physiological role in preventing stress-induced ROS and ethylene accumulation and the resultant cell damage. 相似文献
12.
Changhyun Choi Young Ho Park Soon Il Kwon Chunghyo Yun Ilpyung Ahn Sang Ryeol Park Duk-Ju Hwang 《Plant biotechnology reports》2014,8(2):183-192
The necrotrophic pathogen Pectobacterium carotovorum ssp. carotovorum (Pcc) causes soft rot in a broad range of plant hosts. Approximately 60,000 independent seeds from Arabidopsis activation tagging lines were inoculated with Pcc and screened for resistant mutants. An Rpe1 (resistance protein to Pectobacterium 1) mutant, which had more resistance to Pcc than wild-type (WT) plants, was selected for further study. The T-DNA inserting locus in Rpe1 was located on the middle of chromosome V by flanking sequence analysis. Through expression analysis with several genes adjacent to the T-DNA tagging region, AtWRKY75 gene was highly up-regulated in the Rpe1 mutant compared to the WT plant. The up-regulation of AtWRKY75 gene was shown to be correlated on the induction of the PDF1.2, VSP1 and PR1 genes compared to the WT plant. AtWRKY75 over-expression lines exhibited reduced Pcc bacterial growth compared to WT. Taken together, our data suggest that AtWRKY75 should be a positive regulator in the JA- or SA-mediated defense signaling responses to Pcc. 相似文献
13.
14.
Quettier AL Bertrand C Habricot Y Miginiac E Agnes C Jeannette E Maldiney R 《The Plant journal : for cell and molecular biology》2006,47(5):711-719
The plant hormone abscisic acid (ABA) controls numerous physiological traits: dormancy and germination of seeds, senescence and resistance to abiotic stresses. In order to get more insight into the role of protein tyrosine phosphatase (PTP) in ABA signalling, we obtained eight homozygous T-DNA insertion lines in Arabidopsis thaliana PTP genes. One mutant, named phs1-3, exhibited a strong ABA-induced inhibition of germination as only 26% of its seeds germinated after 3 days instead of 92% for the Columbia (Col-0) line. Genetic and molecular analyses of phs1-3 showed that it bears a unique T-DNA insertion in the promoter of the gene and that the mutation is recessive. PHS1 expression in the mutant is about half that of the Col-0 line. The upregulation of two ABA-induced genes (At5g06760, RAB18) and the downregulation of two ABA-repressed genes (AtCLC-A, ACL) are enhanced in the phs1-3 mutant compared with the wild-type. The 'in planta' aperture of phs1-3 stomata is reduced and the inhibition of the light-induced opening of stomata by ABA is stronger in phs1-3 leaves than in Col-0 leaves. Finally, PHS1 expression is upregulated in the presence of ABA in both phs1-3 and Col-0 but more intensively in the mutant. Thus, phs1-3 is hypersensitive to ABA. Taken together, these results show that PHS1, which encodes a dual-specificity PTP, is a negative regulator of ABA signalling. 相似文献
15.
16.
Guihua Lu Xiping Wang Junhua Liu Kun Yu Yang Gao Haiyan Liu Changgui Wang Wei Wang Guokui Wang Min Liu Guanfan Mao Binfeng Li Jianying Qin Mian Xia Junli Zhou Jingmei Liu Shuqin Jiang Hua Mo Jinteng Cui Nobuhiro Nagasawa Shoba Sivasankar Marc C. Albertsen Hajime Sakai Barbara J. Mazur Michael W. Lassner Richard M. Broglie 《Plant cell reports》2014,33(4):617-631
Key message
A high-quality rice activation tagging population has been developed and screened for drought-tolerant lines using various water stress assays. One drought-tolerant line activated two rice glutamate receptor-like genes. Transgenic overexpression of the rice glutamate receptor-like genes conferred drought tolerance to rice and Arabidopsis.Abstract
Rice (Oryza sativa) is a multi-billion dollar crop grown in more than one hundred countries, as well as a useful functional genetic tool for trait discovery. We have developed a population of more than 200,000 activation-tagged rice lines for use in forward genetic screens to identify genes that improve drought tolerance and other traits that improve yield and agronomic productivity. The population has an expected coverage of more than 90 % of rice genes. About 80 % of the lines have a single T-DNA insertion locus and this molecular feature simplifies gene identification. One of the lines identified in our screens, AH01486, exhibits improved drought tolerance. The AH01486 T-DNA locus is located in a region with two glutamate receptor-like genes. Constitutive overexpression of either glutamate receptor-like gene significantly enhances the drought tolerance of rice and Arabidopsis, thus revealing a novel function of this important gene family in plant biology. 相似文献17.
Tetsuya Kobayashi Kimiko Yamamoto Yoshitaka Suetsugu Seigo Kuwazaki Makoto Hattori Jirapong Jairin Sachiyo Sanada-Morimura Masaya Matsumura 《Proceedings. Biological sciences / The Royal Society》2014,281(1787)
Host plant resistance has been widely used for controlling the major rice pest brown planthopper (BPH, Nilaparvata lugens). However, adaptation of the wild BPH population to resistance limits the effective use of resistant rice varieties. Quantitative trait locus (QTL) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene Bph1. QTL analysis in iso-female BPH lines with single-nucleotide polymorphism (SNP) markers detected a single region on the 10th linkage group responsible for the virulence. The QTL explained from 57 to 84% of the total phenotypic variation. Bulked segregant analysis with next-generation sequencing in F2 progenies identified five SNPs genetically linked to the virulence. These analyses showed that virulence to Bph1 was controlled by a single recessive gene. In contrast to previous studies, the gene-for-gene relationship between the major resistance gene Bph1 and virulence gene of BPH was confirmed. Identified markers are available for map-based cloning of the major gene controlling BPH virulence to rice resistance. 相似文献
18.
Xiang-Qian Zhang Pei Hou Hai-Tao Zhu Guo-Dong Li Xin-Guo Liu Xin-Ming Xie 《Molecular biology reports》2013,40(5):3475-3481
In both yeast and mammals, the major constituent of the endosomal sorting complex required for transport-II (ESCRT-II) is the VPS22/EAP30 protein, which plays an important role in ubiquitin-mediated degradation of membrane proteins through the multivesicular body pathway. However, the functions of ESCRT-II subunits in plants are largely unknown. In this work, we report the genetic analysis and phenotypic characterization of mutants in OsVPS22 gene, which encodes a functional VPS22 homolog in rice. On the basis of a collection of T-DNA lines, we identified a T-DNA insertion mutant, which showed abnormal segregation ratios; we then found that the T-DNA insertion is located within the sixth intron of the OsVPS22 gene. Compared with the wild type, this vps22 mutant exhibited seedling lethality and severe reduction in shoot and root growth. In addition, the vps22 mutant had a chalky endosperm in the grain. In summary, our data suggest that OsVPS22 may be required for seedling viability and grain filling in rice, thus providing a valuable resource for further exploration of the functions of the ESCRTing machinery in plants. 相似文献
19.
Promoters play key roles in conferring temporal, spatial, chemical, developmental, or environmental regulation of gene expression.
Promoters that are subject to specific regulations are useful for manipulating foreign gene expression in plant cells, tissues,
or organs with desirable patterns and under controlled conditions, and have been important for both basic research and applications
in agriculture biotechnology. Recent advances in genomics technologies have greatly facilitated identification and study of
promoters in a genome scale with high efficiency. Previously we have generated a large T-DNA tagged rice mutant library (TRIM),
in which the T-DNA was designed with a gene/promoter trap system, by placing a promoter-less GUS gene next to the right border of T-DNA. GUS activity screens of this library offer in situ and in planta identifications
and analyses of promoter activities in their native configurations in the rice genome. In the present study, we systematically
performed GUS activity screens of the rice mutant library for genes/promoters constitutively, differentially, or specifically
active in vegetative and reproductive tissues. More than 8,200 lines have been screened, and 11% and 22% of them displayed
GUS staining in vegetative tissues and in flowers, respectively. Among the vegetative tissue active promoters, the ratio of
leaf active versus root active is about 1.6. Interestingly, all the flower active promoters are anther active, but with varied
activities in different flower tissues. To identify tissue specific ABA/stress up-regulated promoters, we compared microarray
data of ABA/stress induced genes with those of tissue-specific expression determined by promoter trap GUS staining. Following
this approach, we showed that the peroxidase 1 gene promoter was ABA up-regulated by 4 fold within 1 day of exposure to ABA and its expression is lateral root specific.
We suggest that this be an easy bioinformatics approach in identifying tissue/cell type specific promoters that are up-regulated
by hormones or other factors.
Su-May Yu and Swee-Suak Ko equally contributed to this work. 相似文献