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1.
Background and AimsNitrogen-use efficiency (NUE) of cereals needs to be improved by nitrogen (N) management, traditional plant breeding methods and/or biotechnology, while maintaining or, optimally, increasing crop yields. The aims of this study were to compare spring-barley genotypes grown on different nitrogen levels in field and growth-chamber conditions to determine the effects on N uptake (NUpE) and N utilization efficiency (NUtE) and ultimately, NUE. MethodsMorphological characteristics, seed yield and metabolite levels of 12 spring barley ( Hordeum vulgare) genotypes were compared when grown at high and low nitrogen levels in field conditions during the 2007 and 2008 Canadian growing seasons, and in potted and hydroponic growth-chamber conditions. Genotypic NUpE, NUtE and NUE were calculated and compared between field and growth-chamber environments. Key ResultsGrowth chamber and field tests generally showed consistent NUE characteristics. In the field, Vivar, Excel and Ponoka, showed high NUE phenotypes across years and N levels. Vivar also had high NUE in growth-chamber trials, showing NUE across complex to simplistic growth environments. With the high NUE genotypes grown at low N in the field, NUtE predominates over NUpE. N metabolism-associated amino acid levels were different between roots (elevated glutamine) and shoots (elevated glutamate and alanine) of hydroponically grown genotypes. In field trials, metabolite levels were different between Kasota grown at high N (elevated glutamine) and Kasota at low N plus Vivar at either N condition. ConclusionsDetermining which trait(s) or gene(s) to target to improve barley NUE is important and can be facilitated using simplified growth approaches to help determine the NUE phenotype of various genotypes. The genotypes studied showed similar growth and NUE characteristics across field and growth-chamber tests demonstrating that simplified, low-variable growth environments can help pinpoint genetic targets for improving spring barley NUE. 相似文献
2.
Breeding high-yielding and nutrient-efficient cultivars is one strategy to simultaneously resolve the problems of food security,resource shortage,and environmental pollution.However,the potential increased yield and reduction in fertilizer input achievable by using high-yielding and nutrient-efficient cultivars is unclear.In the present study,we evaluated the yield and nitrogen use efficiency(NUE) of 40 commercial maize hybrids at five locations in North and Northeast China in 2008 and 2009.The effect of interaction between genotype and nitrogen(N) input on maize yield was significant when the yield reduction under low-N treatment was 25%-60%.Based on the average yields achieved with high or low N application,the tested cultivars were classified into four types based on their NUE:efficient-efficient(EE) were efficient under both low and high N inputs,high-N efficient(HNE) under only high N input,low-N efficient(LNE) under only low N input,and nonefficient-nonefficient under neither low nor high N inputs.Under high N application,EE and HNE cultivars could potentially increase maize yield by 8%-10% and reduce N input by 16%-21%.Under low N application,LNE cultivars could potentially increase maize yield by 12%.We concluded that breeding for N-efficient cultivars is a feasible strategy to increase maize yield and/or reduce N input. 相似文献
4.
In the last 40 years the amount of synthetic nitrogen (N) applied to crops has risen drastically, resulting in significant increases in yield but with considerable impacts on the environment. A requirement for crops that require decreased N fertilizer levels has been recognized in the call for a ‘Second Green Revolution’ and research in the field of nitrogen use efficiency (NUE) has continued to grow. This has prompted a search to identify genes that improve the NUE of crop plants, with candidate NUE genes existing in pathways relating to N uptake, assimilation, amino acid biosynthesis, C/N storage and metabolism, signalling and regulation of N metabolism and translocation, remobilization and senescence. Herein is a review of the approaches taken to determine possible NUE candidate genes, an overview of experimental study of these genes as effectors of NUE in both cereal and non‐cereal plants and the processes of commercialization of enhanced NUE crop plants. Patents issued regarding increased NUE in plants as well as gene pyramiding studies are also discussed as well as future directions of NUE research. 相似文献
5.
Phomopsis liquidambari can establish a mutualistic symbiotic relationship with rice. It promotes the growth and yield of the host plant and reduces the amount of nitrogen (N) fertilizer required for plant growth. However, the mechanisms responsible for the effects of the fungal endophyte on N use in rice are largely unknown. We conducted a hydroponic experiment to investigate the effects of P. liquidambari on N uptake and N metabolism in rice plants. Rice plants were cultivated in the presence or absence of P. liquidambari under three N levels. Under the low-N treatment, fungal infection significantly increased the biomass, and the total N, soluble protein, free amino acid, free NH 4 +, and chlorophyll contents of rice roots and shoots. The activities of nitrate reductase and glutamine synthetase were increased in infected rice plants. Some genes related to N uptake ( OsAMT1;1, OsAMT1;3, OsAMT2;2, OsAMT3;2, OsAMT3;3, OsNRT2;1) and N metabolism ( OsNR1, OsGS1, OsGS2, OsNADH- GOGAT) were also up-regulated in infected plants under the low-N treatment. However, these effects gradually weakened as the N level increased. The colonization rate of the endophyte substantially decreased with increasing N levels. Taken together, these results suggest that low-N fertilization induces a physiological state in rice that is favorable for the P. liquidambari symbiosis. The greater extent of P. liquidambari colonization under low-N conditions stimulated the expression of several genes involved in N uptake and N metabolism in rice, thereby enhancing N utilization. These results have implications for enhancing plant growth in low-input systems at nutrient-poor sites. 相似文献
6.
Breeding maize varieties for high nitrogen (N) use efficiency (NUE) by marker-assisted selection using NUE quantitative trait
locus (QTL) or by genetic transfer of NUE-associated genes is a viable approach for increasing grain yield in N-limited production
areas. In this investigation, we evaluated a set of introgression line populations under N supply and N deficiency conditions.
From 42 QTLs for grain yield and yield components, 23 were identified under N supply conditions and 33 from N limited conditions.
Meta-analysis of published maize NUE QTLs revealed 37 “consensus” QTLs, of which, 18 was detected under low N conditions.
In addition, 258 unique ESTs associated with low N stress response, N uptake, transport, and assimilation were aligned on
the maize genome by in silico mapping. Integrating the EST map with the QTL map has resulted in the identification of candidate
NUE-associated genes of the following functional categories: N uptake, transport, and assimilation; carbon (C) metabolism
and assimilation; and cascades of stress response and signal transduction genes. Nine candidates that have been introgressed
into Ye478 significantly altered grain yield/yield components. It is suggested that the dynamics of interactions between C
and N metabolism are important for maize yield. A high NUE variety should have a highly efficient C assimilation per unit
N and actively express CO 2 assimilation-related genes under N-limited conditions. 相似文献
7.
Improving nitrogen use efficiency (NUE) under salt stress has become crucial for rice as it is increasingly facing two major environmental constraints: excessive nitrogen fertilization and soil salinization. However, the interaction between salinity and N levels is very complex and has not yet been considered from the perspective of reduced nitrogen input. We conducted a hydroponic experiment at the early tillering stage on the Yoshida solution to evaluate the impact of rising NaCl and decreasing N application on NUE of four rice cultivars cultivated under three NaCl (0, 56, and 113 mM) and four N (2.86, 1.43, 0.72, and 0.36 mM) concentrations. After 4 weeks, physiological NUE (pNUE), absorption NUE (aNUE), agronomical NUE (agNUE), N transport efficiency (NTE), and physiological traits were evaluated. Significant interactions between N and NaCl-applied concentrations were found in all measured parameters. In all cultivars, increasing the NaCl-applied concentration markedly decreased aNUE and agNUE. For each NaCl treatment, lowering the N applied sharply increased aNUE and agNUE, and this effect was stronger when the NaCl applied was higher. The effect of N lowering on pNUE depended on the NaCl treatment: it enhanced pNUE in the absence of NaCl but had no influence under the highest NaCl-applied concentration. Cultivars largely differed in response to NaCl. The aNUE—but not pNUE—differed between salt-tolerant and salt-sensitive cultivars: aNUE markedly decreased with NaCl concentration in the most salt-sensitive cultivar, whereas it was the highest at the intermediate NaCl concentration in the most salt-tolerant cultivar, especially under low N levels. This finding suggests that under salt conditions, the use of salt-tolerant rice genotypes combined with reducing N level application is necessary to improve NUE. The study of NUE in rice should be focused on the improvement of aNUE with a strong emphasis on the salt tolerance of cultivars. 相似文献
9.
Streptomyces lividans is considered an interesting host for the secretory production of heterologous proteins. To obtain a good secretion yield of heterologous proteins, the availability of suitable nitrogen sources in the medium is required. Often, undefined mixtures of amino acids are used to improve protein yields. However, the understanding of amino acid utilization as well as their contribution to the heterologous protein synthesis is poor.In this paper, amino acid utilization by wild type and recombinant S. lividans TK24 growing on a minimal medium supplemented with casamino acids is profiled by intensive analysis of the exometabolome (metabolic footprint) as a function of time. Dynamics of biomass, substrates, by-products and heterologous protein are characterized, analyzed and compared. As an exemplary protein mouse Tumor Necrosis Factor Alpha (mTNF-α) is considered.Results unveil preferential glutamate and aspartate assimilation, together with glucose and ammonium, but the associated high biomass growth rate is unfavorable for protein production. Excretion of organic acids as well as alanine is observed. Pyruvate and alanine overflow point at an imbalance between carbon and nitrogen catabolism and biosynthetic fluxes. Lactate secretion is probably related to clump formation. Heterologous protein production induces a slowdown in growth, denser clump formation and a shift in metabolism, as reflected in the altered substrate requirements and overflow pattern. Besides glutamate and aspartate, most amino acids are catabolized, however, their exact contribution in heterologous protein production could not be seized from macroscopic quantities.The metabolic footprints presented in this paper provide a first insight into the impact and relevance of amino acids on biomass growth and protein production. Type and availability of substrates together with biomass growth rate and morphology affect the protein secretion efficiency and should be optimally controlled, e.g., by appropriate medium formulation and substrate dosing. Overflow metabolism as well as high biomass growth rates must be avoided because they reduce protein yields. Further investigation of the intracellular metabolic fluxes should be conducted to fully unravel and identify ways to relieve the metabolic burden of plasmid maintenance and heterologous protein production and to prevent overflow. 相似文献
10.
The objective of this study was to evaluate the effects of oak tannin extract ( OTE) added in forage before ensiling on dairy cows fed at 92% of their digestible protein requirements. Six multiparous lactating Holstein cows were used in a crossover design (two treatments × two periods). The control treatment ( CON) was based on a diet including 50% of grass silage, whereas the experimental treatment ( TAN) included grass silage sprayed with OTE (26 g/kg DM) just before baling. Milk yield (on average 24 kg fat protein corrected milk per day) was not affected, but both milk and rumen fatty acids profiles were impacted by OTE. Nitrogen intake (415 g N per cow per day) and nitrogen use efficiency ( NUE; 0.25 on average) were not affected, but a shift from urine (−8% of N intake relatively to control, P = 0.06) to faecal N (+5%; P = 0.004) was observed with the TAN diet ( P ≤ 0.05). Nitrogen apparent digestibility was thus reduced for TAN (−3%; P ≤ 0.05). The effect of OTE on ruminal and milk FA profiles suggests an impact on rumen microbiota. Nitrogen isotopic discrimination between animal proteins and diet ( Δ15N) was evaluated as a proxy for NUE. While no differences in NUE were observed across diets, a lower Δ 15N of plasma proteins was found when comparing TAN v. CON diets. This finding supports the concept that Δ 15N would mainly sign the N partitioning at the metabolic level rather than the overall NUE, with the latter also being impacted by digestive processes. Our results agree with a N shift from urine to faeces, and this strategy can thus be adopted to decrease the environmental impact of ruminant protein feeding. 相似文献
12.
Key message By comparing 195 varieties in eight trials, this study assesses nitrogen use efficiency improvement in high and low nitrogen conditions in European winter wheat over the last 25 years. Abstract In a context where European agriculture practices have to deal with environmental concerns and nitrogen (N) fertiliser cost, nitrogen use efficiency (NUE) has to be improved. This study assessed genetic progress in winter wheat ( Triticum aestivum L.) NUE. Two hundred and twenty-five European elite varieties were tested in four environments under two levels of N. Global genetic progress was assessed on additive genetic values and on genotype × N interaction, covering 25 years of European breeding. To avoid sampling bias, quality, precocity and plant height were added as covariates in the analyses when needed. Genotype × environment interactions were highly significant for all the traits studied to such an extent that no additive genetic effect was detected on N uptake. Genotype × N interactions were significant for yield, grain protein content (GPC), N concentration in straw, N utilisation, and NUE. Grain yield improvement (+0.45 % year ?1) was independent of the N treatment. GPC was stable, thus grain nitrogen yield was improved (+0.39 % year ?1). Genetic progress on N harvest index (+0.12 % year ?1) and on N concentration in straw (?0.52 % year ?1) possibly revealed improvement in N remobilisation. There has been an improvement of NUE additive genetic value (+0.33 % year ?1) linked to better N utilisation (+0.20 % year ?1). Improved yield stability was detected as a significant improvement of NUE in low compared to high N conditions. The application of these results to breeding programs is discussed. 相似文献
14.
O-linked N-acetyl-β- d-glucosamine ( O-GlcNAc) is a ubiquitous and dynamic post-translational modification known to modify over 3,000 nuclear, cytoplasmic, and mitochondrial eukaryotic proteins. Addition of O-GlcNAc to proteins is catalyzed by the O-GlcNAc transferase and is removed by a neutral- N-acetyl-β-glucosaminidase ( O-GlcNAcase). O-GlcNAc is thought to regulate proteins in a manner analogous to protein phosphorylation, and the cycling of this carbohydrate modification regulates many cellular functions such as the cellular stress response. Diverse forms of cellular stress and tissue injury result in enhanced O-GlcNAc modification, or O-GlcNAcylation, of numerous intracellular proteins. Stress-induced O-GlcNAcylation appears to promote cell/tissue survival by regulating a multitude of biological processes including: the phosphoinositide 3-kinase/Akt pathway, heat shock protein expression, calcium homeostasis, levels of reactive oxygen species, ER stress, protein stability, mitochondrial dynamics, and inflammation. Here, we will discuss the regulation of these processes by O-GlcNAc and the impact of such regulation on survival in models of ischemia reperfusion injury and trauma hemorrhage. We will also discuss the misregulation of O-GlcNAc in diseases commonly associated with the stress response, namely Alzheimer’s and Parkinson’s diseases. Finally, we will highlight recent advancements in the tools and technologies used to study the O-GlcNAc modification. 相似文献
15.
The use of nitrogen (N) fertilizers in agriculture has a great ability to increase crop productivity. However, their excessive use has detrimental effects on the environment. Therefore, it is necessary to develop crop varieties with improved nitrogen use efficiency (NUE) that require less N but have substantial yields. Orphan crops such as millets are cultivated in limited regions and are well adapted to lower input conditions. Therefore, they serve as a rich source of beneficial traits that can be transferred into major crops to improve their NUE. This review highlights the tremendous potential of systems biology to unravel the enzymes and pathways involved in the N metabolism of millets, which can open new possibilities to generate transgenic crops with improved NUE. 相似文献
18.
Background and aims Nickel (Ni) has a critical role in the urea metabolism of plants. This study investigated the impact of seed Ni content along with external Ni supply on the growth, various nitrogen (N) metabolites and N use efficiency (NUE) of soybean plants. Methods Soybean plants raised from Ni-poor or Ni-rich seeds were grown in nutrient solution with or without external Ni supply and fed with either urea or nitrate as the sole N source. The changes in growth, leaf chlorophyll levels, Ni and N concentrations of different plant parts, tissue accumulation of various N metabolites and N uptake of soybean as well as NUE and its components were examined. Results Nickel starvation reduced the shoot biomass of urea-fed plants by 25 % and the leaf chlorophyll levels by up to 35 %, but nitrate-fed plants were unaffected. Visual toxicity symptoms were not observed in urea-fed plants. Under urea supply, Ni-deficient plants had lower levels of total N, protein and free amino acids in various organs. Root uptake of urea was severely depressed in Ni-deprived plants. Availability of Ni did not have any effect on the NUE of nitrate-fed plants, whereas its deficiency reduced the NUE of urea-fed plants by 30 %. The growth and N nutritional status of urea-fed soybean were significantly improved by high seed Ni reserves as well as external Ni supply. Conclusion Adequate Ni supply is required for maximizing the growth, root uptake of urea and NUE of urea-fed plants. Seed Ni reserves contribute significantly to the Ni and thus N nutritional status of soybean. 相似文献
20.
Nitrogen (N) uptake and nitrogen use efficiency (NUE) are closely related through feedback mechanisms to soil N availability and N cycling in forested ecosystems. We investigated N uptake and NUE not only at the leaf, litterfall, and aboveground levels but also belowground and whole stand levels along a topographic gradient of soil N availability in a cool temperate deciduous forest in Japan. In this study, we addressed how whole stand level N uptake and NUE affect C and N cycling in forested ecosystems. At the leaf, litterfall, and aboveground levels, N uptake decreased and NUE increased with decreasing soil N availability. This pattern resulted from decreasing leaf N concentrations and increasing N resorption efficiencies as soil N availability declined. Low N concentrations in litterfall may have resulted in little soil N being available to plants, due to microbial immobilization. In contrast, when belowground components were included, N uptake and NUE were not correlated with soil N availability. This was mainly due to higher levels of fine root production when soil N availability was low. Higher fine root allocation can result in a high input of detritus to decomposer systems and, thus, contribute to accumulation of soil organic matter and immobilization by microbes, which may result in further soil N availability decline. Our results suggest that allocation to the fine root rather than whole stand level NUE is important for C and N cycling in forested ecosystems, as is the feedback mechanism in which litterfall level NUE shifts with changes in the N concentration of litterfall. 相似文献
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