Influence of Bacillus cereus on microbiocenosis of gastrointestinal tract in rats

The modifying effect of Bacillus cereus on intestinal microbiocenosis was investigated in eubiotic and disbiotic female rats. Qualitative and quantitative characteristics of gut and mucosal microflora from different parts of rats' intestine were studied before and after intragastral application of B. cereus suspension. The single application of B. cereus suspension resulted in appearance of this bacterium in feces and in parietal mucin from all parts of the intestine. In eubiotic rats compared with disbiotic, B. cereus adhere to parietal mucin much more efficiently and supplanted indigenous microflora. During disbiosis B. cereus sometimes had stimulating effect on the intestinal microbiocenosis. Gut microbiocenosis appeared to be more resistant to B. cereus invasion than mucosal. This fact was considered to be the evidence of higher sensitivity of mucosal microbiocenosis to short-term influence of exogenous microbial factor.     

Microbiocenosis of parietal mucin in the gastrointestinal tract of rats

The qualitative and quantitative composition of the microbial community in parietal mucin at different areas of the gastrointestinal tract (GIT) of rats was revealed. The pronounced variability in the quantitative and qualitative characteristics of microbiocenosis in parietal mucin of rats at different sections was revealed. The differences were most pronounced in the passage from upper to lower GIT sections, the large intestine found to be the richest biocenosis. The microbial composition of rat feces was faintly associated with the GIT parietal microbiocenosis. The individual areas of GIT mucosa were unique of their microbial characteristics and organization. This makes it possible to regard them as relatively independent biotopes and indicates that it is impossible to evaluate the microbial community by one of the colonic mucosal sifes.     

Bacterial vaginosis: etiopathogenetic aspects

Current microbiological data on the vaginal microecology in healthy women and in patients with bacterial vaginosis are presented in the article. Problems of formation of colonization resistance of vaginal microbiocenosis were discussed. Etiologic role of certain microorganisms and their associations in the pathogenesis of bacterial vaginosis was considered. Pathophysiological processes leading to development of vaginal disbiosis, features of local immune status and molecular specificity of intercellular interactions in the development of adaptive immune response were characterized.     

Microecology and humoral immunity characteristics of the vagina in women with nonspecific inflammatory diseases of the genitals

The complex cytological, microbiological and immunological examination of 90 female patients with nonspecific inflammatory diseases of the genital tracts and 30 clinically healthy women (the control group) was carried out. The examination revealed significant microbiocenosis changes in the lumen and the parietal area of the vagina, depending on the severity of the infectious process: a decrease in the level of lactobacilli, an increase in the content of opportunistic facultative anaerobic microorganism in the lumen and obligate anaerobic bacteria in the parietal area. Pronounced correlation between the content of opportunistic microorganisms and the levels of IgM and IgA, as well as secretory IgA and free secretory component in vaginal secretions in patients in patients with non-specific inflammatory diseases of the genital tracts has been revealed.     

The autologous intestinal microflora in the postresuscitation period after acute blood loss in rats

In this work the data obtained in the study of intestinal microbiocenosis in rats on the third day of the postresuscitation period after acute blood loss are presented. The quantitative and qualitative shifts of microflora at different biotopes, such as the wall of the small intestine, the parietal microflora of Peyer's patches, the contents and wall of the large intestine, have been characterized. Some specific features of dysbiotic changes have been revealed in comparison with the shifts of intestinal microflora in cases of dysbacteriosis caused by other reasons. The translocation of different microorganisms, including Bacterium bifidum and lactobacilli, into mesenteric lymph nodes, the liver, the spleen and the blood has been observed.     

Formation of intestinal microflora in children during the first year of their life

In 525 young children the state of intestinal microbiocenosis was studied every month of the first year their life. The study revealed that the process of the microflora formation lasted throughout the first year of their life and was characterized by dysbiotic disturbances. During this period the aggravation of dysbiotic changes in the intestine of these children on months 3, 6-7 and 11-12 was of particular importance. The formation of stable dysbacteriosis led to a decrease in the immunological status of the child, which was manifested by the increased content of such microorganisms as hemolytic cocci, Proteus and a decrease in the quantitative level of bifidobacteria in the total intestinal microbiocenosis by the end of the first year of child's life.     

Microflora of gastrointestinal tract in patients with chronic cholecystitis

AIM: Assessment of quantitative and qualitative characteristics of gastrointestinal microflora in patients with chronic cholecystitis. MATERIALS AND METHODS: Bacteriological analysis of biopsy samples of esophageal and gastric mucosae as well as stool samples was performed in 10 healthy volunteers and 28 patients with chronic cholecystitis. RESULTS: Opportunistic microorganisms are acquiring greater significance in formation of gastrointestinal microbiocenosis. Significant increase of both isolation rate and density of gastrointestinal tract colonization by staphylococci, enterobacteriae and anaerobic microorganisms was revealed. CONCLUSION: Obtained results showed abnormalities of microbiocenosis of esophageal and gastric mucosae and presence of intestinal disbacteriosis of different degrees of intensity.     

Characteristics of changes in microbiocenosis in patients with chronic nonspecific urethritis

The species composition and the complex of biological characteristics of microflora in the front section of urethra in healthy males and in patients with chronic nonspecific urethritis. The study revealed that in patients with chronic nonspecific nongonococcal urethritis changes in the microbiocenosis of the urethra were observed. These changes were manifested by a decrease in the number of species, the appearance of Grain-negative enterobacterial flora and an increase in the persistence potential of symbiotic bacteria. These disturbances are regarded as the manifestation of urogenital dysbiosis.     

Study of the parietal microflora in the rat intestine

The article deals with the adaptation of the existing method of the isolation of microorganisms from parietal mucin of the intestine of experimental animals (rats) with a view to ensure the methodological uniformity of investigations. The effectiveness of the method of sparing disintegration of parietal mucin taken from the mucous membrane of the gastrointestinal tract, specially developed for human biomaterial, and its adequacy for microbiological investigations of the digestive organs of rats have been confirmed. A certain similarity between the microbiocenosis of the parietal mucin in the intestine of humans and rats has been established.     

Microbiocenosis of nasal mucous membrane during allergic rhinosinusitis

Overall quantity of microorganisms and their relative prevalence on nasal mucous membrane in patients with allergic rhinosinusitis are determined. Microbial flora in studied group was characterized qualitatively and quantitatively. Obtained results showed that there were changes of microbiocenosis of nasal mucous membrane during allergic rhinosinusitis and in characteristics of species in Staphylococcus genus.     

Microbiologicaland immunological criteria for estimation of ureaplasmosis treatment efficacy in women

The role of surface and parietal vaginal microbiocenosis and immunoglobulins in development of infectious process, in prediction of resistance of causative microorganism to antibiotics and treatment efficacy was demonstrated on group of women with ureaplasmosis. Evaluation of anaerobic part of vaginal microbiota contemporary with its aerobic part reliably increased informative value of microbiological test in women with urogenital diseases. There are reasons to include interferon preparations into the complex therapy of ureaplasmosis.     

Population characteristics and biological properties of micrococci colonizing the skin of the breasts

As shown in this investigation, the population characteristics of micrococci inhabiting the skin of the mammary glands make it possible to regard these microorganisms as the permanent members of microbiocenosis. Their proportion among all isolated strains was, respectively, 27.2% and 27.7% for nulliparous and pregnant women and their occurrence, 96.7% and 92.1%. The largest population of micrococci was registered at the nipple area of both left (934-1,956 colony-forming units per sq. cm) and right (1,092-2,134 colony-forming units per sq. cm) breasts. A decrease in the number of micrococci was observed at the above-mentioned areas during pregnancy, which was accompanied by changes in the specific and antagonistic profiles of the association. This occurred supposedly under the necessity of preserving its dynamic stability.     

A method for assessing the microflora status of the large intestine using a computer

The qualitative and quantitative composition of the microflora of the large intestine has been studied in 31 healthy adults and in 137 patients with acute viral hepatitides A and B. A set of quantitative tests ensuring the complete characterization of the microbiocenosis under study has been proposed. The results obtained in this investigation have been processed by means of a computer with the use of the principles of numeric taxonomy, thus making it possible to obtain the objective criterion of the state of the microflora of the large intestine, expressed by the similarity index.     

Microbiota of the large bowel in patients with allergic diseases

Spectrum of microorganisms vegetated in the large bowel of patients with allergic diseases was investigated. The index of constancy C was used for characterization of the microbial population. 80 patients (31 adults and 49 children < 14 years) were examined. Most prominent changes in obligatory and facultative parts of microflora were revealed in children. Serological typing of 108 cultures of enterobacteria isolated in children under examination with disbiosis showed variety of serogroups that can testify to the presence of pathogenic variants.     

Possible use of total blood analysis for diagnosis of intestinal dysbacteriosis accompanied by bacteremia

Possible application of total blood analysis in the diagnosis of intestinal dysbacteriosis of the 2nd--3d degree, accompanied by bacteriemia, is substantiated. The proposed method includes the calculation of tentative coefficients of peripheral blood characteristics. These coefficients reflect changes in the intestinal microbiocenosis and make it possible to make conclusions on the presence of bacteriemia. This method is recommended for use as the screening test in the laboratory diagnostics of bacteriemia.     

Dynamics of some characteristics of humoral immunity,vaginal interferon status in pregnant women with the relapse of chronic pyelonephritis

65 pregnant women with the exacerbation of chronic pyelonephritis in the III trimester of gestation and 34 healthy pregnant women were examined. The quantitative content of immunoglobulins, the activity of interferon in cervico = vaginal washings and the composition of the vaginal microflora were determined. All patients with the relapse of chronic pyelonephritis exhibited disturbances in the normal microbiocenosis of the genitals and the dysfunction of the local immunity of the genital system, accompanied with a decrease in serum and secretory IgA, an increase in the amount of IgG and IgM, increased interferon activity. Pregnant women with the relapse of chronic pyelonephritis received, in addition to traditional therapy, local treatment with Kipferon suppositories, an immunomodulating preparation. The study revealed that the use of this preparation normalized the characteristics of local immunity, the composition of the microflora' of the genitals and led to the disappearance of the clinical symptoms of the disease.     

Characteristics of associated microflora of ejaculate from males with gonococcal infections

Species composition and biological properties of aerobic and anaerobic microflora from an ejaculate from males with acute and chronic gonococcal infection and from healthy men were studied. Patients with gonococcal infection were shown to present quantitative and qualitative changes in microbiocenosis of the reproductive tract. Patients with acute gonorrhea displayed decreased species diversity in contrast to healthy persons, while different species of associated with gonococci microbes with high persistent potential, were cultured in patients suffering from a chronic form of infection. The role of revealed pathological biocenosis patterns in developing chronic infection as well as postgonorrheal gonococci-free urethritis is discussed.     

Cortical Reorganization after Hand Immobilization: The beta qEEG Spectral Coherence Evidences

There is increasing evidence that hand immobilization is associated with various changes in the brain. Indeed, beta band coherence is strongly related to motor act and sensitive stimuli. In this study we investigate the electrophysiological and cortical changes that occur when subjects are submitted to hand immobilization. We hypothesized that beta coherence oscillations act as a mechanism underlying inter- and intra-hemispheric changes. As a methodology for our study fifteen healthy individuals between the ages of 20 and 30 years were subjected to a right index finger task before and after hand immobilization while their brain activity pattern was recorded using quantitative electroencephalography. This analysis revealed that hand immobilization caused changes in frontal, central and parietal areas of the brain. The main findings showed a lower beta-2 band in frontal regions and greater cortical activity in central and parietal areas. In summary, the coherence increased in the frontal, central and parietal cortex, due to hand immobilization and it adjusted the brains functioning, which had been disrupted by the procedure. Moreover, the brain adaptation upon hand immobilization of the subjects involved inter- and intra-hemispheric changes.     

Age-dependent characteristics of intestinal microbiocenosis in Kemerovo residents

In 873 residents of Kemerovo aged 1-60 years and older the microflora of the large intestine was studied. The study revealed that in all examined subjects dysbiotic changes in the composition of normal microflora were observed. More profound disturbances of microbiocenosis were found in children aged 1-2 years, in adolescents and in adults over 60 years of age.     

The characteristics of the vaginal microflora in intrauterine contraception

The results of the study of vaginal microbiocenosis in women using intrauterine contraceptives are presented. Inflammatory complications in intrauterine contraception were shown to be linked with vaginal dysbiosis. Disturbances in vaginal microbiocenosis were characterized by the deficiency of lactoflora and the presence of enterobacteria, Staphylococcus aureus, Gardnerella of fungi of the genus Candida. The problem of the possibility of complications of microbial etiology in women using intrauterine contraceptives are discussed.