共查询到20条相似文献,搜索用时 15 毫秒
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Soybean DREB1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression
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Satoshi Kidokoro Keitaro Watanabe Teppei Ohori Takashi Moriwaki Kyonoshin Maruyama Junya Mizoi Nang Myint Phyu Sin Htwe Yasunari Fujita Sachiko Sekita Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2015,81(3):505-518
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Molecular control mechanisms for abiotic stress tolerance are based on the activation and regulation of specific stress-related
genes. The phytohormone abscisic acid (ABA) is a key endogenous messenger in a plant’s response to such stresses. A novel
ABA binding mechanism which plays a key role in plant cell signaling cascades has recently been uncovered. In the absence
of ABA, a type 2C protein phosphatase (PP2C) interacts and inhibits the kinase SnRK2. Binding of ABA to the PYR/PYLs receptors
enables interaction between the ABA receptor and the PP2C protein, and abrogates the SnRK2 inactivation. The active SnRK2
is then free to activate the ABA-responsive element Binding Factors which target ABA-dependent gene expression. We used the
grape as a model to study the ABA perception mechanism in fruit trees. The grape ABA signaling cascade consists of at least
seven ABA receptors and six PP2Cs. We used a yeast two-hybrid system to examine physical interaction in vitro between the
grape ABA receptors and their interacting partners, and found that twenty-two receptor-PP2C interactions can occur. Moreover,
quantifying these affinities by the use of the LacZ reporter enables us to show that VvPP2C4 and VvPP2C9 are the major binding partners of the ABA receptor. We also tested in vivo the root and leaf gene expression of the various
ABA receptors and PP2Cs in the presence of exogenic ABA and under different abiotic stresses such as high salt concentration,
cold and drought, and found that many of these genes are regulated by such abiotic environmental factors. Our results indicate
organ specificity in the ABA receptor genes and stress specificity in the VvPP2Cs. We suggest that VvPP2C4 is the major PP2C involved in ABA perception in leaves and roots, and VvRCAR6 and VvRCAR5 respectively, are the major receptors involved in ABA perception in these organs. Identification, characterization and manipulation
of the central players in the ABA signaling cascades in fruit trees is likely to prove essential for improving their performance
in the future. 相似文献
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Expression and promoter activity of the desiccation-specific Solanum tuberosum gene, StDS2 总被引:1,自引:0,他引:1
Environmental stresses induce the expression of several plant genes via multiple and cross‐talking signalling pathways. Previously it was shown that ScDS2, a gene of the wild potato species, Solanum chacoense, is highly inducible by dehydration but not by abscisic acid (ABA), the mediator of many plant stress responses. Herein it is shown that ScDS2‐related genes are present in the cultivated potato, Solanum tuberosum (StDS2) and also in the non‐tuberizing Solanum species, Solanum brevidens (SbDS2). We show that expression of StDS2 is dehydration‐specific, is not inducible by cold, heat, salt, hypoxia or oxidative stresses, and is independent of ABA. Signalling of StDS2 induction, however, is dependent on the synthesis of novel proteins because cycloheximide can block StDS2 expression. To analyse the promoter region of StDS2 a genomic library of Solanum tuberosum was established and 1140 and 498 bp regions of the StDS2 promoter were isolated. The promoter fragments were fused to the β‐glucuronidase (GUS) reporter gene and tested in transgenic potato plants. Both promoter fragments were able to induce GUS activity in response to dehydration. This result suggests that drought‐specific cis‐elements are located within 498 bp upstream to the StDS2 coding sequence. 相似文献
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Tomohiro Kiyosue Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki 《Plant molecular biology》1994,25(5):791-798
InArabidopsis thaliana L., accumulation of abscisic acid (ABA) began to increase 2 h after plants had been subjected to dehydration stress and reached maximum levels after 10h. Differential hybridization was used to isolate 26Arabidopsis cDNAs with gene expression induced by a 1 h dehydration treatment. The cDNA clones were classified into 16 groups based on Southern blot hybridization, and named ERD (early-responsive todehydration) clones. Partial sequencing of the cDNA clones revealed that three ERDs were identical to those of HSP cognates (Athsp70-1, Athsp81-2, and ubiquitin extension protein). Dehydration stress strongly induced the expression of genes for the three ERDs, while application of ABA, which is known to act as a signal transmitter in dehydration-stressed plants, did not significantly affect the ERD gene expression. This result suggests that these HSP cognates are preferentially responsive to dehydration stress inA. thaliana, and that signaling pathways for the expression of these genes under conditions of dehydration stress are not mainly mediated by ABA. We also discuss the possible functions of these three ERD gene products against dehydration stress. 相似文献
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Thompson AJ Jackson AC Parker RA Morpeth DR Burbidge A Taylor IB 《Plant molecular biology》2000,42(6):833-845
Two genes encoding enzymes in the abscisic acid (ABA) biosynthesis pathway, zeaxanthin epoxidase (ZEP) and 9-cis-epoxycarotenoid dioxygenase (NCED), have previously been cloned by transposon tagging in Nicotiana plumbaginifolia and maize respectively. We demonstrate that antisense down-regulation of the tomato gene LeZEP1 causes accumulation of zeaxanthin in leaves, suggesting that this gene also encodes ZEP. LeNCED1 is known to encode NCED from characterization of a null mutation (notabilis) in tomato. We have used LeZEP1 and LeNCED1 as probes to study gene expression in leaves and roots of whole plants given drought treatments, during light/dark cycles, and during dehydration of detached leaves. During drought stress, NCED mRNA increased in both leaves and roots, whereas ZEP mRNA increased in roots but not leaves. When detached leaves were dehydrated, NCED mRNA responded rapidly to small reductions in water content. Using a detached leaf system with ABA-deficient mutants and ABA feeding, we investigated the possibility that NCED mRNA is regulated by the end product of the pathway, ABA, but found no evidence that this is the case. We also describe strong diurnal expression patterns for both ZEP and NCED, with the two genes displaying distinctly different patterns. ZEP mRNA oscillated with a phase very similar to light-harvesting complex II (LHCII) mRNA, and oscillations continued in a 48 h dark period. NCED mRNA oscillated with a different phase and remained low during a 48 h dark period. Implications for regulation of water stress-induced ABA biosynthesis are discussed. 相似文献
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A RhABF2/Ferritin module affects rose (Rosa hybrida) petal dehydration tolerance and senescence by modulating iron levels
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Jitao Liu Youwei Fan Jing Zou Yiqun Fang Linghao Wang Meng Wang Xinqiang Jiang Yiqing Liu Junping Gao Changqing Zhang 《The Plant journal : for cell and molecular biology》2017,92(6):1157-1169
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Heat shock factor C2a serves as a proactive mechanism for heat protection in developing grains in wheat via an ABA‐mediated regulatory pathway
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Xiao‐Jun Hu Dandan Chen C. Lynne Mclntyre M. Fernanda Dreccer Zheng‐Bin Zhang Janneke Drenth Sundaravelpandian Kalaipandian Hongping Chang Gang‐Ping Xue 《Plant, cell & environment》2018,41(1):79-98
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