Seed survival and periodicity of seedling emergence in ten species of annual weeds

Seeds or fruits of 10 species of annual weeds were collected in each of 3 years and mixed with the top 7 · 5 cm of sterilised soil (confined in cylinders sunk in the ground outdoors and cultivated three times yearly. The numbers of seedlings emerging were recorded for 5 yr and the numbers of viable seeds remaining then determined. Emergence of Anagallis arvensis, Anchusa arvensis, Chaenorrhinum minus and Euphorbia peplus was mainly in spring; Lamium amplexicaule and Myosotis arvensis also had a spring peak but emergence continued in summer and autumn. Seedlings of Aphanes arvensis appeared almost entirely in autumn and those of Lamium purpureum between May and October. Veronica arvensis had spring and autumn peaks, while Fumaria densiflora showed no definite pattern. Except for Anagallis arvensis and F. densiflora, in which there was apparent innate dormancy due to the seed coat, most seedlings appeared in the first year with a decrease, usually exponential, from year to year. This was most rapid in C. minus and E. peplus, of which few viable seeds remained after 5 yr. Seed survival for the other species ranged from 2·6% of those sown for Anchusa arvensis to 14·8% for F. densiflora.     

Different substitution tendencies of leaf flavones in theVeronica hederifolia group (Scrophulariaceae)

Flavonoid patterns of several provenances ofVeronica triloba, V. sublobata, andV. hederifolia s. str. have been investigated. 7-0-glucuronides of apigenin and luteolin as well as of 6-substituted (6-hydroxyluteolin and hispidulin) and 3′-methylated (chrysoeriol) derivatives represent characteristic accumulation tendencies in theV. hederifolia group. The three species show slightly different flavonoid profiles.V. hederifolia exhibits a cumulative pattern of all compounds occurring either inV. triloba orV. sublobata, thus supporting the hypothesis of an allopolyploid origin ofV. hederifolia.     

Interspecific variation in persistence of buried weed seeds follows trade‐offs among physiological,chemical, and physical seed defenses

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Adventitious Root Formation in Veronica Spp.

Species of the genus Veronica differ in habitat preferences,growth form and in adventitious root production. The annualspecies rarely or never produce adventitious roots in intactplants in the field but some, for example V. persica and V.arvensis will root vigorously from single node stem segmentsin culture. Others, such as V. agrestis require the presenceof IAA for substantial levels of root formation to occur incultured stem segments. Veronica hederifolia cuttings rarelyproduce roots. Stem cuttings of the perennial species, in general,rooted more vigorously than those of annual plants. Both V.fihiformis and V. serpyllifolia root very strongly. The position of root production from the stem cuttings differedfrom species to species. Roots arose either from the node, theregion of the base or at some intermediate point. Veronica arvensis,V. chamaedrys and V. persica rooted mainly from the basal regionwhereas V. filiformis rooted mainly from the node. Veronicaserpyllifolia cuttings rooted at both of these locations. Veronica filiformis, a perennial species that is infertile inBritain, produces root primordia in intact plants at nodes whichare close to the shoot apex. Thus, even very young stem segmentshave ‘preformed’ root primordia. For this reason,detached stem segments of V. filiformis root very rapidly andthis probably has been of great significance in its successfulinvasion and spread in lawns and short turf areas. Veronica spp., adventitious roots, indol-3-ylacetic acid, root primordia, vegetative reproduction     

Seed survival and periodicity of seedling emergence in eight species of Cruciferae

Seeds of eight species of Cruciferae were collected, usually in each of 3 years, and mixed with the top 7-5 cm of sterilised soil confined in cylinders sunk in the ground outdoors and cultivated three times yearly. The seedlings emerging were recorded for 5 yr and the numbers of viable seeds remaining then determined. Emergence of Alliaria petiolata was almost entirely restricted to February and March. That of Erysimum cheiranthoides, Lepidium campestre, Sinapis arvensis and Raphanus raphanistrum took place mainly in March and April, but seedlings continued to appear until late autumn. Some seedlings of Arabidopsis thaliana, Cardamine hirsuta and Sisymbrium officinale appeared in spring, but most did so in summer (C. hirsuta and S. officinale) or early autumn (A. thaliana). Seed survival in A. petiolata was of short duration and there were few seedlings after the second year, whereas 18% of the seeds of R. raphanistrum were still present and viable after 5 yr. Seed survival at this time for the other six species ranged from 1 -6% (C. hirsuta) to 6-1% (S. arvensis).     

Seed survival and periodicity of seedling emergence in some species of Atriplex, Chenopodium, Polygonum and Rumex

Seeds or fruits of 10 weed species were collected in each of 3 years and mixed with the top 7·5 cm of sterilised soil which was confined in cylinders sunk in the ground outdoors and cultivated three times yearly. The numbers of seedlings emerging were recorded for 5 yr and the numbers of viable seeds remaining then determined. Few, if any, seedlings of Atriplex, Chenopodium or Polygonum spp. emerged in the autumn of sowing; the main emergence was consistently in March–May (A. hastata, A. patula), April–May (P. lapathifolium, P. persicaria), April–June (C. polyspermum), May–June (C. rubrum) or June–August (C. hybridum). When sown before November, some seedlings of Rumex crispus, R. obtusifolius and R. sanguineus appeared immediately. Most emerged in April in subsequent years. Seedling emergence was usually greatest in the first year; thereafter, with all species, seedling numbers decreased approximately exponentially from year to year. The mean numbers of seeds found to be viable after 5 yr ranged from 2·5% (R. sanguineus) to 16·6% (C. rubrum) of those sown.     

Topsoil Handling and Storage Effects on Woodland Restoration in Western Australia

An analysis of the effects of topsoil handling and storage methods was undertaken to optimize the potential rehabilitation of southwest Western Australian Banksia woodland species present before site disturbance. An increase in the depth of topsoil stripped from the Banksia woodland, from 10 to 30 cm, correlated to decreasing seedling recruitment from the soil seedbank by a factor of three following in situ respreading in an area to be restored. There was no significant difference in total seedling recruitment in situ at two depths of spread, 10 cm and 30 cm. These results concur with an ex situ trial on the effects of depth of seed burial on seedling recruitment that showed most species failed to emerge from depths greater than 2 cm. In situ stockpiling of the woodland topsoil for 1 or 3 years demonstrated a substantial and significant decline in seedling recruitment to 54% and 34% of the recruitment achieved in fresh topsoil, respectively. Stripping and spreading during winter substantially depressed seedling recruitment, compared with autumn operations, as did in situ stockpiling followed by spreading in the wet season, or stockpiling in winter followed by spreading in spring. No loss in total seedling recruitment occurred when replaced topsoil and subsoil were ripped to 80 cm following spreading of topsoil in sites to be restored. Conclusions from this study are that (1) topsoil provides a useful source of seeds for rehabilitation of Banksia woodland communities in the southwest of Western Australia, (2) correct handling of the topsoil, stripped and replaced fresh and dry (autumn direct return) to the maximum depths of 10 cm, can be used to optimize revegetation of species‐rich plant communities with this type of seedbank, and (3) ripping of topsoil and subsoil to ease compaction of newly restored soils does not diminish the recruitment potential of the soil seedbank in the replaced topsoil.     

The dynamics of experimental arable weed communities under different management practices

Abstract. Weed communities, comprising 12 introduced species at constant starting densities and three species already present in the seed bank, were followed through three years of continuous winter wheat. The wheat and weeds were subjected to two treatments in a split-plot factorial design, organic contrasting with conventional fertilizer, and ploughing plus hand-roguing contrasting with minimum tillage plus herbicide. The minimum tilled plots developed in a uniform manner, and became dominated by very high densities of Anisantha sterilis. Agrostemma githago and Galium aparine also persisted in these plots at lower densities. The ploughed plots had a lower total density but a greater range of species. Stellaria media, Veronica persica and Avena fatua were the most common; other species occurred at lower densities. The major effect of fertilizer treatment was a greater initial increase by G. aparine on the organic, minimum tilled plots compared with the conventionally fertilized, minimum tilled plots. Species associated with minimum tillage were annuals with either no or a short term seed bank and autumn germination and rather predictable dynamics, whereas species that did well under ploughing were either spring germinating or had a persistent seed bank, implying greater annual variation in population size associated with weather conditions. There seemed no clear way to distinguish between those species which were abundant on the ploughed plots and those which were scarce under all conditions using readily accessible data.     

Rabbit endozoochroy and seedbank build-up in an acidic grassland

The sources of seed for seedbank build-up in an acidic grassland were identified from analysis of differences in seedbank build-up over one year between plots where the input of rabbit pellets to seed-free soil had either been left or removed. In parallel, the flux of seed arriving in rabbit pellets was monitored. Pellet seed content and total seed input were highest in late summer/early autumn and again in the spring. The seed content of the pellets was dominated by a small number of species: Sagina apetala, Senecio jacobaea, Urtica dioica and Veronica arvensis. Smaller seeded species were more likely to be present as germinable seed in the pellets. Seedbank build-up as a result of wind, splash or adhesive dispersal totalled 547 seedsm². The additional effect of allowing seed input in pellets was 267 seedsm², though this increase was not significant. The total increase in seedbank over one year was equivalent to 15–20% of the seedbank present in undisturbed soil. Eight species showed a significant build-up of seed in the seedbank over one year as a result of all means of dispersal, but only Myosotis discolor showed a significantly higher soil germinable seed content in the plots where pellets had been allowed to remain in situ. The build-up of seed in the seedbank is contrasted with the build-up of vegetation on disturbed areas within the same system.     

The role of weed hosts and the distribution and activity of vector nematodes in the ecology of tobacco rattle virus

At a site in eastern Scotland, nine common species of arable weeds were infected with tobacco rattle virus (TRV), and some of these, notably Viola arvensis and Stellaria media, comprised an overwintering reservoir of the virus. TRV was seed-borne both in naturally and in experimentally infected V. arvensis (2–10%), and occasionally in other weed species. In the glasshouse at 20 ^oC a naturally infective population of vector nematodes (Tricho-dorus spp.) kept in soil free of plants retained its infectivity for 20 wk, although few Trichodorus survived for this period. In the field, the incidence of TRV infection in potato (spraing disease) in plots kept free of weeds for 1–5 years was 3–4 times that in weed-infested plots but Trichodorus numbers did not differ appreciably between the two treatments. Presumably the virus is retained for long periods in its vectors and these feed on potato more frequently when other hosts are not available. Weeds are probably important in the long term as hosts of both TRV and its vectors, but in the short term weed control seems unlikely to prevent potato spraing because of the long persistence of TRV in vector populations. In the field, Trichodorus accumulated near the interface between topsoil and subsoil, and the incidence of spraing was greatest where the topsoil was shallowest. When cucumber seedlings were exposed to virus-carrying Trichodorus, TRV reached a greater concentration in roots at 20 ^oC than at 24 ^oC, and the virus was not detected in roots at 29 ^oC. In a sandy soil, TRV was transmitted only when the water content exceeded 15%, and at least 30 % water was needed for maximum transmission. Annual records of rainfall and spraing disease suggest that spraing is most prevalent when the summer is wettest. TRV is not confined to cultivated land. Stabilized sand dunes supporting a pure stand of Ammophila armaria were colonized by Trichodorus pachyder-mus, but TRV was detected only where the plant community had enlarged to include V. arvensis and other dicotyledons. In such situations, TRV may be introduced in the seed of V. arvensis, and the movement of soil by wind probably contributes to the dispersal of Trichodorus.     

Differential effect of drought regimes on the seedling performance of six floodplain grassland species

• The performance of seedlings is crucial for the survival and persistence of plant populations. Although drought frequently occurs in floodplains and can cause seedling mortality, studies on the effects of drought on seedlings of floodplain grasslands are scarce. We tested the hypotheses that drought reduces aboveground biomass, total biomass, plant height, number of leaves, leaf area and specific leaf area (SLA), and increases root biomass and root‐mass fraction (RMF) and that seedlings from species of wet floodplain grasslands are more affected by drought than species of dry grasslands.
• In a greenhouse study, we exposed seedlings of three confamilial pairs of species (Pimpinella saxifraga, Selinum carvifolia, Veronica teucrium, Veronica maritima, Sanguisorba minor, Sanguisorba officinalis) to increasing drought treatments. Within each plant family, one species is characteristic of wet and one of dry floodplain grasslands, confamilial in order to avoid phylogenetic bias of the results.
• In accordance with our hypotheses, drought conditions reduced aboveground biomass, total biomass, plant height, number of leaves and leaf area. Contrary to our hypotheses, drought conditions increased SLA and decreased root biomass and RMF of seedlings. Beyond the effects of the families, the results were species‐specific (V. maritima being the most sensitive species) and habitat‐specific. Species indicative of wet floodplain grasslands appear to be more sensitive to drought than species indicative of dry grasslands.
• Because of species‐ and habitat‐specific responses to reduced water availability, future drought periods due to climate change may severely affect some species from dry and wet habitats, while others may be unaffected.

     

Seedling bank dynamics of Fagus grandifolia var. mexicana before and after a mast year in a Mexican cloud forest

Abstract. Fagus grandifolia var. mexicana seedling dynamics were studied in a relict forest inside the crater of a volcano where it is the only canopy tree species. The objectives of the study were (1) to determine changes in seedling density before and after a mast year, as well as to monitor seedling recruitment, mortality and growth rate in relation to the micro‐environment, and (2) to discuss the impact of masting on seedling dynamics of the population. Before masting, seedling density was 8.8 seedlings m^–2, afterwards seedling density peaked at 51.5 seedlings m^–2, then decreased by 50% in 3 months and by 85% after 8 months, down to levels of before the mast year (9.9 seedlings m^–2) after 16 months. Seedlings in the forest floor before the mast‐seeding event showed a low relative growth rate – 0.14 mm mm^–1 mo^–1 against 1.27 mm mm^–1 mo^–1 for seedlings that emerged immediately after masting. Seed germination was high (83%) and rapid (L₅₀= 7 days) following masting. Seedling growth was positively correlated with soil water content but not with temperatures and relative humidity. Data suggested that seed production in mast years is important in maintaining the seedling bank, and thus the viability of relict Fagus populations.     

Direct seeding of Brazilian savanna trees: effects of plant cover and fertilization on seedling establishment and growth

Direct seeding is a promising technique for ecological restoration, but it has been poorly studied in neotropical savannas. Different types of plant cover (no cover, crops, or green manure) and fertilization (unfertilized, synthetic fertilizer, or poultry litter) were used to verify if survival and growth of different tree species after direct seeding could be enhanced by the use of any combination of these techniques. Seedling emergence, establishment, and growth were followed for 2 years for six savanna tree species sown in an agricultural field in Central Brazil. Germination was high (52%, on average) for Anacardium occidentale, Aspidosperma macrocarpon, Hymenaea stigonocarpa, Dipteryx alata, Eugenia dysenterica, and Magonia pubescens. Six additional species were planted, but less than 5% of these seeds germinated. Crops (60% shade) did not affect seedling survival and biomass compared with the control treatment, supporting the use of this strategy during the initial phase of restoration to involve farmers in the process. In contrast, the excessive shading (95%) from the green manure treatment decreased the survival of two species and the growth in biomass and diameter of five species, especially when combined with fertilization. Seedling growth was very slow throughout the experiment, requiring extended weed management. This study supports the use of direct seeding of the studied species for savanna restoration, but methods could be improved to include a larger number of species.     

Chemical composition of Thuja orientalis L. essential oils and study of their allelopathic potential on germination and seedling growth of weeds

The chemical composition of essential oils isolated by hydrodistillation from needles and cones of Thuja orientalis L. was analysed by gas chromatography-flame ionisation detection and gas chromatography–mass spectrometry. Twenty-one compounds were identified; quantitative differences, mainly, between cone and needle oils were observed. Both oils were rich in monoterpene hydrocarbons and the major constituents were α-pinene (64.2 and 49.3%, respectively, in cones and needles), β-phellandrene (6.7–9.6%) and α-cedrol (3.9 and 8.2%). Herbicidal properties of Thuja essential oils were assessed against three invasive weed species in crops: Sinapis arvensis L., Phalaris paradoxa L. and Lolium rigidum Gaud. The study of herbicidal activity was carried out on the weed germination, seed vigour and seedling growth. Our results showed a strong inhibitory effect on all tested weeds following a dose-dependent manner, property that could be valourised in managing biocontrol of weeds.     

Effects of cover crops, weeds and plant debris on development of Mycocentrospora acerina in caraway

This paper reports on the search for inoculum sources of Mycocentrospora acerina on caraway (Carum carvi L.). Obvious suspects are cover crops of biennial caraway and preceding crops of annual caraway. Other suspects are weeds in or alongside the field. Finally, survival structures of the fungus, chlamydospore chains, packed in plant debris or naked, are suspected. M. acerina is able to infect many plant species, including cover crops of caraway such as spinach for seed production and peas. However, the agronomical suitability of a crop to serve as a cover crop of biennial caraway proved to be a more important factor in determining caraway yield than the susceptibility of the cover crop to M. acerina. This finding was corroborated by the fact that spinach and peas as preceding crops had no significant effects on M. acerina development in spring caraway sown the next year. Dill, barley and four weed species were found as new hosts of M. acerina. The role of weed hosts, susceptible crops and plant debris in the survival of the fungus in years without caraway is discussed. Caraway sown on soil containing infested caraway straw, infested debris of other plant species or chlamydospores grown in pure culture, became infected by M. acerina. Only high inoculum densities of chlamydospores in the soil caused severe damping-off of caraway seedlings. The opportunity for disease management by agronomical means is quite limited.     

Cereal weeds variation in middle Egypt: Role of crop family in weed composition

Cereals occupies a major part in the diet of humans globally, participating more to our daily protein and calorie intake than any other crop. The present study highlight the weed flora of cereal crops compared to other crops in middle Egypt and their distribution. Ninety-two weed species were recorded in the all studied crops, cereal and other crops; in the studied area belong to 67 genera and 20 families. Egyptian clover; showed the highest numbers of both weed species and genera followed by wheat, on contrast the lowest weed species and genera numbers were recorded associated with Solanaceous crops tomato and pepper. Wheat crops exhibited the highest number of weed species, among cereals, followed by maize crop, while the lowest weed species number was detected in barley crop. Chenopodium murale, Cynodon dactylon, Convolvulus arvensis and Malva parviflora were the most frequent species in winter cereals, while Echinochloa colona, P. oleraceae were the most frequent weeds in summer cereals. Chorological analysis of the recorded weed species showed that cosmopolitan elements showed the highest numbers in total weed flora Differences in weed species compositions were fundamentally influenced by seasonal priority. Based on TWINSPAN and Ward classifications, crop family showed slightly effect as a factor affecting weed composition.     

The effects of burning on the understorey composition of 11–13 year-old rehabilitated bauxite mines in Western Australia – Vegetation characteristics

Alcoa of Australia Limited has been rehabilitating bauxite mines in the jarrah (Eucalyptus marginata) forest of Western Australia for more than 30 years. Mines rehabilitated in the early 1980s using out-dated methods have built up substantial fuel loads that may be reduced through prescribed burning. The vegetation response of 11–13 year-old rehabilitated bauxite mines to fire regimes differing in intensity and season over the first two years of post-burn succession is compared to the native jarrah forest. A total of 243 species from 137 genera and 56 families were identified in the native forest reference sites and in the 11–13 year-old rehabilitated areas before and after burning. The vegetation of the pre-burn rehabilitated areas was very different to that of the native jarrah forest. While total live plant cover, Acacia density, non-native eucalypt seedling density, weed density and the evenness index were similar between the two areas, total plant density, live Acacia cover, the proportion of weeds, native species numbers and diversity were significantly different. However, the greatest difference between the vegetation of the pre-burn rehabilitated sites and the native jarrah forest was the higher dominance of seeding species (plants killed by fire) in rehabilitated areas. In contrast, native jarrah forest was dominated by resprouting species (plants that survive fire). Burning the rehabilitated sites was successful in making the areas more similar to the forest in terms of total plant density, live Acacia cover and native species numbers but decreased their similarity in terms of live plant cover, Acacia density, non-native eucalypt seedling density, weed density and evenness. The vegetation response of the rehabilitated areas to different seasons of burning showed that autumn burning led to a greater increase in plant establishment than spring burning. Autumn burning also resulted in an undesirable increase in the density of non-native eucalypt seedlings that was not observed following spring burning. Although burning these 11–13 year-old rehabilitated sites will increase similarity to the native forest, it is unlikely that they will resemble the native jarrah forest without further management intervention.     

Seed-borne cucumber mosaic virus infection of narrow-leafed lupin (Lupinus angustifolius) in Western Australia

In 1986 in Western Australia, cucumber mosaic virus (CMV) infection was widespread in breeders' selections of narrow-leafed lupin (Lupinus angustifolius), and in collections of lupin cvs and wild L. angustifolius lines. When seed of some of these selections and cvs was sown, seed-borne CMV was detected in seedlings. Infection of F₁ progenies was traced to use of infected parent plants. CMV was also widespread in 25 seed crops of the new lupin cv. Wandoo but not in 42 seed crops of the new cv. Danja. When samples of the seed sown in 1986 were tested, CMV was detected in 3 - 34% of seedlings of cv. Wandoo but in none of cv. Danja. Following intensive roguing of symptom-bearing plants in the 1986 seed crop of new lupin cv. Gungurru, the level of seedling infection with CMV in seed samples after harvest was 0·1-0·2%. CMV was detected in 6 - 8%, 0·6-5% and 0 - 18% of seedlings from seed samples of established lupin cvs Chittick, Yandee and Illyarrie respectively. Highest levels of seed transmission were in seed from crops grown in high rainfall areas. When a sample of cv. Wandoo seed was graded for size by sieving, CMV was detected in seedlings grown from seed in all grades, but the smallest grade contained the highest level of infection. When seed was collected from pods at different positions on plants in a CMV-infected crop of cv. Illyarrie, seed from primary pods transmitted the virus to seedlings at a 3% rate, seed from first order lateral pods at 8% while seed from second and third order lateral pods transmitted at 13%. Examination of CMV-infected lupin crops indicated that seed-infected plants competed poorly and tended to be shaded out in dense crops but to survive in sparse crops. In 1987 during drought conditions after seeding, plant mortality was greater with seed-infected seedlings than with healthy seedlings despite wide plant spacing. An isolate of CMV from subterranean clover (Trifolium subterraneum) induced severer symptoms in lupins than four isolates from lupin; only the subterranean clover isolate prevented seed production. In tests at one lupin breeding site, CMV was found in 15 species of weeds and volunteer legumes. Fumaria officinalis, Stachys arvensis and volunteer lupins were most frequently infected.     

Tallgrass prairie restoration: implications of increased atmospheric nitrogen deposition when site preparation minimizes adventive grasses

Site preparation designed to exhaust the soil seedbank of adventive species can improve the success of tallgrass prairie restoration. Despite these efforts, increased rates of atmospheric nitrogen (N) deposition over the next century could potentially promote the growth of nitrophilic, adventive species in tallgrass restoration projects. We used a field experiment to examine how N addition affected species composition and plant productivity over the first 3 years of a tallgrass prairie restoration that was preceded by the planting of glyphosate‐resistant crops and multiple applications of glyphosate to exhaust the pre‐existing seedbank. We predicted that N addition would increase the percent cover of adventive plant species not included in the original seeding. Contrary to our prediction, only the cover of native species increased with N addition; native non‐leguminous forbs increased substantially, with Conyza canadensis (a weedy native species not part of the restoration seed mix) exploiting the combination of high N and bare ground in the first year, and non‐leguminous forbs (in particular Monarda fistulosa) and native C₃ grasses, all of which were seeded, increasing with N addition by the third year. Native legumes was the only functional group that exhibited lower cover in N addition plots than in control plots. There was no significant response by native C₄ grasses to N addition, and adventive grasses remained mostly absent from the plots. Overall, our results suggest that site pre‐treatment with herbicide may continue to be effective in minimizing adventive grasses in restored tallgrass prairie, despite future increases in atmospheric N deposition.     

A model for the impact of herbicide tolerance on the performance of oilseed rape as a volunteer weed

The introduction of genetically modified, herbicide-tolerant, oilseed rape into the agricultural environment will have ramifications beyond weed control of the crop. Herbicide-tolerant rape will undoubtedly become part of established volunteer weed populations that occur in many cereal rotations, but its longevity in these populations and its impact as a weed and contaminant of future oilseed rape crops is uncertain. A life cycle model of volunteer oilseed rape was therefore constructed, incorporating existing information on physiological processes such as emergence pattern, longevity of buried seed, death rates of various structures and flowering and seeding as functions of density. The model was designed to allow interaction with control factors such as harvesting efficiency, herbicide treatment, ploughing and the sequence of crops in the rotation. Many of the physiological parameters (including seed decay rates, fecundity at high density) are uncertain, simply through lack of information in the appropriate context. Other parameters such as harvesting efficiency and herbicide kill rates, are inherently variable in farming. Accordingly, a Monte-Carlo approach, in which the model was run many times with different random realisations of parameter sets, was used to expose factors to which the seedbank was sensitive. Sets of 1250 realisations were compared for each of two extreme conditions: where herbicide could be used according to current intensive farming practice and where it was not an option (representing total herbicide tolerance). Modelled seedbank numbers after 5 yr ranged from 10^-3to 10⁴m^-2, realistic values found in arable soils. The use of herbicide, together with efficient harvesting of seed, clearly has an important suppressive effect on the oilseed rape seedbank, keeping it lower than 10²m^-2(a typical sowing rate) after 5 yr in more than 80% of realisations. In the absence of herbicide, seedbanks were invariably greater, but their absolute value depended strongly on harvesting efficiency and the extent to which high density of plants suppressed fecundity. Analysis of the time series from the simulations showed that the seedbank levels fluctuated by orders in magnitude from year to year in the absence of herbicide use. The sensitivity analysis of the life-cycle model led to the development of a simplified model for the seedbank dynamics. The model shows that the essential features of the dynamics result from an interaction between density-dependent fecundity and the perturbations due to management. Therefore predictions of the effect of herbicide tolerance on seedbank dynamics are highly uncertain without knowledge of the density dependence of fecundity. Furthermore, the sensitivity to management practices suggests that seedbank levels will be substantially more difficult to control if the efficacy of herbicide is compromised. It is concluded that the model and Monte-Carlo approach have many potential uses in exploring the effects of management, cultivar physiology and the nature of the transgenes.