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1.
Chen W Zhang Y Liu X Chen B Tu J Tingdong F 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):849-858
The inheritance of yield-related traits in rapeseed (Brassica napus) is poorly understood, and the investigations on mapping of quantitative trait loci (QTL) for such traits are only few. QTL
related to six traits were mapped which include plant height (PH), height of lowest primary effective branch (HPB), length
of main inflorescence (LMI), silique length (SL), number of primary branches (FB) and silique density (SD). A set of 258 doubled
haploid (DH) lines derivatives of a cross between a canola variety Quantum and a resynthesized B. napus line No.2127-17, and a fixed immortalized F2 (designated as IF2) population generated by randomly permutated intermating of these DHs were investigated. A genetic linkage map was constructed
using 208 SSR and 189 SRAP markers for the DH population. Phenotypic data were collected from three environments for the two
populations. Using composite interval mapping analyses, 30 and 22 significant QTL were repeatedly detected across environments
for the six traits in the DH and IF2 populations, respectively. Twenty-nine QTL were common between the two populations. The directions of parental contribution
for all common QTL were the same, showing a great potential for marker-assisted selection in improving these traits. Some
chromosomal regions harbor QTL for multiple traits, which were consistent with significant phenotypic correlations observed
among traits. The results provided a better understanding of the genetic factors controlling yield-related traits in rapeseed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
Mapping QTLs with digenic epistasis under multiple environments and predicting heterosis based on QTL effects 总被引:2,自引:0,他引:2
Gao YM Zhu J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(3):325-333
Mixed linear model approach was proposed for mapping QTLs with the digenic epistasis and QTL by environment (QE) interaction
as well as additive and dominant effects. Monte Carlo simulations indicated that the proposed method could provide unbiased
estimations for both positions and genetic main effects of QTLs, as well as unbiased predictions for QE interaction effects.
A method was suggested for predicting heterosis based on individual QTL effects. The immortalized F2 (IF2) population constructed by random mating among RI or DH lines is appropriate for mapping QTLs with epistasis and their QE
interaction. Based on the models and methodology proposed, we developed a QTL mapping software, QTLMapper 2.0 on the basis
of QTLmapper 1.0, which is suitable for analyzing populations of DH, RIL, F2 and IF2. Data of thousand grain weight of IF2 population with 240 lines derived from elite hybrid rice Shanyou 63 were analyzed as a worked example. 相似文献
3.
Genetic dissection of chlorophyll content at different growth stages in common wheat 总被引:3,自引:0,他引:3
Quantitative trait loci (QTLs) for chlorophyll content were studied using a doubled haploid (DH) population with 168 progeny
lines, derived from a cross between two elite Chinese wheat cultivars Huapei 3 × Yumai 57. Chlorophyll content was evaluated
at the maximum tillering stage (MS), the heading stage (HS), and the grain filling stage (GS), at three different environments
in 2005 and 2006 cropping seasons. QTL analyses were performed using a mixed linear model approach. A total of 17 additive
QTLs and nine pairs of epistatic QTLs were detected. Ten of 17 additive QTLs for chlorophyll content were persistently expressed
at more than two growth stages, which suggest developmentally regulated loci controlling genetics for chlorophyll content
in different growth stages in wheat. One novel major QTL for chlorophyll content was closely linked with the PCR marker Xwmc215 and was persistently expressed at three growth stages. 相似文献
4.
Villalta I Bernet GP Carbonell EA Asins MJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(6):1001-1017
Salt tolerance has been analysed in two populations of F7 lines developed from a salt sensitive genotype of Solanum lycopersicum var. cerasiforme, as female parent, and two salt tolerant lines, as male parents, from S. pimpinellifolium, the P population (142 lines), and S. cheesmaniae, the C population (116 lines). Salinity effects on 19 quantitative traits including fruit yield were investigated by correlation,
principal component analysis, ANOVA and QTL analysis. A total of 153 and 124 markers were genotyped in the P and C populations,
respectively. Some flowering time and salt tolerance candidate genes were included. Since most traits deviated from a normal
distribution, results based on the Kruskal–Wallis non-parametric test were preferred. Interval mapping methodology and ANOVA
were also used for QTL detection. Eight out of 15 QTLs at each population were detected for the target traits under both control
and high salinity conditions, and among them, only average fruit weight (FW) and fruit number (FN) QTLs (fw1.1, fw2.1 and fn1.2) were detected in both populations. The individual contribution of QTLs were, in general, low. After leaf chloride concentration,
flowering time is the trait most affected by salinity because different QTLs are detected and some of their QTL×E interactions
have been found significant. Also reinforcing the interest on information provided by QTL analysis, it has been found that
non-correlated traits may present QTL(s) that are associated with the same marker. A few salinity specific QTLs for fruit
yield, not associated with detrimental effects, might be used to increase tomato salt tolerance. The beneficial allele at
two of them, fw8.1 (in C) and tw8.1 (for total fruit weight in P) corresponds to the salt sensitive parent, suggesting that the effect of the genetic background
is crucial to breed for wide adaptation using wild germplasm. 相似文献
5.
A major QTL for powdery mildew resistance is stable over time and at two development stages in winter wheat 总被引:9,自引:0,他引:9
N. Chantret D. Mingeot P. Sourdille M. Bernard J. M. Jacquemin G. Doussinault 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(6-7):962-971
Despite the large impact of powdery mildew in wheat cultivated areas, little has been done to study powdery mildew resistance
by QTL analysis up to now. The objective of the present paper is to present how the genetic basis of powdery mildew resistance
in the resistant wheat line RE714 have been studied by QTL analysis at the adult plant stage over the course of 3 years, and
at the vernalized seedling plant stage, and a comparison between the results obtained. Two segregating populations (DH and
F2:3) were derived from the cross between the resistant line (RE714), and a susceptible line (Hardi); these were analysed for
powdery mildew resistance at the adult plant stage in the field under natural infection conditions in 1996, 1997 and 1998.
The DH population was also tested for powdery mildew resistance at the vernalized seedling stage with four different isolates
of powdery mildew. At the adult plant stage, a total of three QTLs (on chromosomes 5D, 4A and 6A) and five QTLs (on chromosomes
5D, 6A, 7A and 7B) were found for the DH and F2:3 populations, respectively. The genetic control of resistance was found to be polygenic but involved a major QTL (on chromosome
5D), which was detected each year and which explained a high proportion of the variability observed (28.1%–37.9%). At the
vernalized seedling stage, two QTLs were found (on chromosomes 5D and 7B) and the QTL detected on chromosome 5D was common
to the four isolates tested. The comparison between the two development stages showed that the QTL on chromosome 5D was detected
in all the different environments tested and again explained a high proportion of the variability. Different molecular interpretations
of this QTL have also been discussed.
Received: 5 October 2000 / Accepted: 1 March 2001 相似文献
6.
Several biologically significant parameters that are related to rice tillering are closely associated with rice grain yield.
Although identification of the genes that control rice tillering and therefore influence crop yield would be valuable for
rice production management and genetic improvement, these genes remain largely unidentified. In this study, we carried out
functional mapping of quantitative trait loci (QTLs) for rice tillering in 129 doubled haploid lines, which were derived from
a cross between IR64 and Azucena. We measured the average number of tillers in each plot at seven developmental stages and
fit the growth trajectory of rice tillering with the Wang–Lan–Ding mathematical model. Four biologically meaningful parameters
in this model––the potential maximum for tiller number (K), the optimum tiller time (t
0), and the increased rate (r), or the reduced rate (c) at the time of deviation from t
0––were our defined variables for multi-marker joint analysis under the framework of penalized maximum likelihood, as well
as composite interval mapping. We detected a total of 27 QTLs that accounted for 2.49–8.54% of the total phenotypic variance.
Nine common QTLs across multi-marker joint analysis and composite interval mapping showed high stability, while one QTL was
environment-specific and three were epistatic. We also identified several genomic segments that are associated with multiple
traits. Our results describe the genetic basis of rice tiller development, enable further marker-assisted selection in rice
cultivar development, and provide useful information for rice production management. 相似文献
7.
The objective of this study was to dissect the genetic control of days to flowering (DTF) and photoperiod sensitivity (PS)
into the various components including the main-effect quantitative trait loci (QTLs), epistatic QTLs and QTL-by-environment
interactions (QEs). Doubled haploid (DH) lines were produced from an F1 between two spring Brassica napus cultivars Hyola 401 and Q2. DTF of the DH lines and parents were investigated in two locations, one location with a short
and the other with a long photoperiod regime over two years. PS was calculated by the delay in DTF under long day as compared
to that under short day. A genetic linkage map was constructed that comprised 248 marker loci including SSR, SRAP, and AFLP
markers. Further QTL analysis resolved the genetic components of flowering time and PS into the main-effect QTLs, epistatic
QTLs, and QEs. A total of 7 main-effect QTLs and 11 digenic interactions involving 21 loci located on 13 out of the 19 linkage
groups were detected for the two traits. Three main-effect QTLs and four pairs of epistatic QTLs were involved in QEs conferring
DTF. One QTL on linkage group (LG) 18 was revealed to simultaneously affect DTF and PS and explain for the highest percentage
of the phenotypic variation. The implications of the results for B. napus breeding have been discussed.
The text was submitted by the authors in English. 相似文献
8.
The crop seed is a complex organ that may be composed of the diploid embryo, the triploid endosperm and the diploid maternal tissues. According to the genetic features of seed characters, two genetic models for mapping quantitative trait loci (QTLs) of crop seed traits are proposed, with inclusion of maternal effects, embryo or endosperm effects of QTL, environmental effects and QTL-by-environment (QE) interactions. The mapping population can be generated either from double back-cross of immortalized F2 (IF2) to the two parents, from random-cross of IF2 or from selfing of IF2 population. Candidate marker intervals potentially harboring QTLs are first selected through one-dimensional scanning across the whole genome. The selected candidate marker intervals are then included in the model as cofactors to control background genetic effects on the putative QTL(s). Finally, a QTL full model is constructed and model selection is conducted to eliminate false positive QTLs. The genetic main effects of QTLs, QE interaction effects and the corresponding P-values are computed by Markov chain Monte Carlo algorithm for Gaussian mixed linear model via Gibbs sampling. Monte Carlo simulations were performed to investigate the reliability and efficiency of the proposed method. The simulation results showed that the proposed method had higher power to accurately detect simulated QTLs and properly estimated effect of these QTLs. To demonstrate the usefulness, the proposed method was used to identify the QTLs underlying fiber percentage in an upland cotton IF2 population. A computer software, QTLNetwork-Seed, was developed for QTL analysis of seed traits. 相似文献
9.
M. M. Alam E. S. Mace E. J. van Oosterom A. Cruickshank C. H. Hunt G. L. Hammer D. R. Jordan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(10):2253-2266
Key message
A QTL model for the genetic control of tillering in sorghum is proposed, presenting new opportunities for sorghum breeders to select germplasm with tillering characteristics appropriate for their target environments.Abstract
Tillering in sorghum can be associated with either the carbon supply–demand (S/D) balance of the plant or an intrinsic propensity to tiller (PTT). Knowledge of the genetic control of tillering could assist breeders in selecting germplasm with tillering characteristics appropriate for their target environments. The aims of this study were to identify QTL for tillering and component traits associated with the S/D balance or PTT, to develop a framework model for the genetic control of tillering in sorghum. Four mapping populations were grown in a number of experiments in south east Queensland, Australia. The QTL analysis suggested that the contribution of traits associated with either the S/D balance or PTT to the genotypic differences in tillering differed among populations. Thirty-four tillering QTL were identified across the populations, of which 15 were novel to this study. Additionally, half of the tillering QTL co-located with QTL for component traits. A comparison of tillering QTL and candidate gene locations identified numerous coincident QTL and gene locations across populations, including the identification of common non-synonymous SNPs in the parental genotypes of two mapping populations in a sorghum homologue of MAX1, a gene involved in the control of tiller bud outgrowth through the production of strigolactones. Combined with a framework for crop physiological processes that underpin genotypic differences in tillering, the co-location of QTL for tillering and component traits and candidate genes allowed the development of a framework QTL model for the genetic control of tillering in sorghum. 相似文献10.
C. Y. Liao P. Wu B. Hu K. K. Yi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(1):104-111
A double-haploid (DH) population and a recombinant inbred (RI) line population, derived from a cross between a tropical japonica variety, Azucena, as male parent and two indica varieties, IR64 and IR1552, as female parents respectively, were used in both field and pot experiments for detecting QTLs
and epistasis for rice panicle number in different genetic backgrounds and different environments. Panicle number (PN) was
measured at maturity. A molecular map with 192 RFLP markers for the DH population and a molecular map with 104 AFLP markers
and 103 RFLP markers for the RI population were constructed, in which 70 RFLP markers were the same. Six QTLs were identified
in the DH population, including two detected from field experiments and four from pot experiments. The two QTLs, mapped on
chromosomes 1 and 12, were identical in both field and pot experiments. In the RI population, nine QTLs were detected, five
QTLs from field conditions and four from the pot experiments. Three of these QTLs were identical in both experimental conditions.
Only one QTL, linked to CDO344 on chromosome 12, was detected across the populations and experiments. Different epistasitic
interaction loci on PN were found under different populations and in different experimental conditions. One locus, flanked
by RG323 and RZ801 on chromosome 1, had an additive effect in the DH population, but epistatic effects in the RI population.
These results indicate that the effect of genetic background on QTLs is greater than that of environments, and epistasis is
more sensitive to genetic background and environments than main-effect QTLs. QTL and epistatic loci could be interchangeable
depending on the genetic backgrounds and probably on the environments where they are identified.
Received: 26 May 2000 / Accepted: 19 October 2000 相似文献
11.
The natural inhibitor proteins IF1 regulate mitochondrial F0F1ATPsynthase in a wide range of species. We characterized the interaction of CaM with purified bovine IF1, two bovine IF1 synthetic peptides, as well as two homologous proteins from yeast, namely IF1 and STF1. Fluorometric analyses showed that bovine and yeast inhibitors bind CaM with a 1:1 stoichiometry in the pH range between
5 and 8 and that CaM-IF1 interaction is Ca2+-dependent. Bovine and yeast IF1 have intermediate binding affinity for CaM, while the Kd (dissociation constant) of the STF1-CaM interaction is slightly higher. Binding studies of CaM with bovine IF1 synthetic peptides allowed us to identify bovine IF1 sequence 33–42 as the putative CaM-binding region. Sequence alignment revealed that this region contains a hydrophobic motif
for CaM binding, highly conserved in both yeast IF1 and STF1 sequences. In addition, the same region in bovine IF1 has an IQ motif for CaM binding, conserved as an IQ-like motif in yeast IF1 but not in STF1. Based on the pH and Ca2+ dependence of IF1 interaction with CaM, we suggest that the complex can be formed outside mitochondria, where CaM could regulate IF1 trafficking or additional IF1 roles not yet clarified. 相似文献
12.
Chuchuan Fan Guangqin Cai Jie Qin Qingyuan Li Minggui Yang Jianzhong Wu Tingdong Fu Kede Liu Yongming Zhou 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(7):1289-1301
Seed weight is an important component of grain yield in oilseed rape (Brassica napus L.), but the genetic basis for the important quantitative trait is still not clear. In order to identify the genes for seed
weight in oilseed rape, QTL mapping for thousand seed weight (TSW) was conducted with a doubled haploid (DH) population and
an F2 population. A complete linkage map of the DH population was constructed using 297 simple sequence repeat (SSR) markers. Among
nine TSW QTLs detected, two major QTLs, TSWA7a and TSWA7b, were stably identified across years and collectively explained 27.6–37.9% of the trait variation in the DH population. No
significant epistatic interactions for TSW detected in the DH population indicate that the seed weight variation may be primarily
attributed to additive effects. The stability and significance of TSWA7a and TSWA7b were further validated in the F2 population with different genetic backgrounds. By cloning BnMINI3a and BnTTG2a, two B. napus homologous genes to Arabidopsis thaliana, allele-specific markers were developed for TSWA5b and TSWA5c, two TSW QTLs on A5, respectively. The importance of the major and minor QTLs identified was further demonstrated by analysis
of the allelic effects on TSW in the DH population. 相似文献
13.
Genetic dissection of interactions between wheat flour starch and its components in two populations using two QTL mapping methods 总被引:1,自引:0,他引:1
Zhiying Deng Wenqi Fang Xin Guo Xinying Zhao Hong Guo Shuna Hu Jichun Tian 《Molecular breeding : new strategies in plant improvement》2018,38(4):41
Starch content and its components are important for determining wheat end-use quality and yield. However, little information is available about their interactions at the QTL/gene level in more than one population using different QTL mapping methods. Therefore, to dissect these interactions, two mapping populations from two locations over 2 years were used. The QTLs for the populations were analyzed by unconditional and conditional QTL mapping by two different analysis methods. In the two populations, there were a total of 24 unconditional additive QTLs detected for flour amylose (FAMS), flour amylopectin (FAMP), flour total starch (FTSC), and the ratio of FAMS to FAMP using ICIMapping4.1 methods, but 26 unconditional QTLs were found using QTLNetwork2.0 methods. Of these QTLs, 10 stable major additive QTLs were identified in more than one environment, mainly distributed on chromosomes 3B, 4A, 5A, and 7D. The maximum percentage of phenotypic variance explained (PVE) reached 54.31%. Two new unconditional major additive QTLs on chromosome 3B (Qftsc3B and Qfamp3B) were found. A total of 23 and 19 conditional additive QTLs were identified in the two populations using two different methods, respectively. Of which, eight and six stable major conditional QTLs were detected on chromosomes 3B, 4A, and 7D, respectively. New repressed QTLs were identified, such as Qftsc/fams5B-1 and Qftsc/fams5B-2. There were 20 epistatic unconditional and 15 conditional QTLs detected. In all, important QTLs on chromosomes 3B, 4A, and 7A were found in both populations. However, the number of important QTLs in the special recombinant inbred line (RIL) population was higher than that in the double haploid (DH) population, especially on chromosomes 7D and 5B. Moreover, the QTLs on chromosomes 4A, 7A, and 7D were close to the Wx-1 loci in the RIL population. These indicated better results can be obtained by a special population to target traits than by a common population. The important QTLs on key chromosomes can always be detected no matter what kinds of populations are used, such as the QTLs on chromosome 4A. In addition, QTL clusters were found on chromosomes 4A, 3B, 7A, 7D, and 5A in the two populations, indicating these chromosome regions were very important for starch biosynthesis. 相似文献
14.
Quantitative trait loci for phyllochron and tillering in rice 总被引:4,自引:0,他引:4
Miyamoto N Goto Y Matsui M Ukai Y Morita M Nemoto K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(4):700-706
Morphogenetic processes in sequentially growing leaves and tiller buds are highly synchronized in rice (Oryza sativa L.). Consequently, the appearance of successive leaves in the main tiller acts as the pacemaker for the whole shoot system development. The time interval between the appearance of successive leaves (days/leaf) in the main tiller is called the phyllochron. The objectives of the investigation reported here were: (1) to identify quantitative trait loci (QTLs) that control rice phyllochron and (2) to understand the roles of phyllochron QTLs as an underlying developmental factor for rice tillering. For this purpose we developed a set of recombinant inbred lines derived from a cross between IR36 (indica) and Genjah Wangkal (tropical japonica). Composite interval mapping detected three phyllochron QTLs located on chromosomes 4, 10 and 11, where the presence of a Genjah Wangkal allele increased phyllochron. The largest QTL (on chromosome 4) was located on the genomic region syntenic to the vicinity of the maize Teopod 2 mutation, while the QTL on chromosome 10 was close to the rice plastochron 1 mutation. These three phyllochron QTLs failed to coincide with major tiller number QTLs. However, one tiller number QTL was associated with small LOD peaks for phyllochron and tiller-bud dormancy that were linked in coupling phase, suggesting that linked small effects of phyllochron and tiller-bud dormancy might result in a multiplicative effect on tiller number. 相似文献
15.
Ma Z Zhao D Zhang C Zhang Z Xue S Lin F Kong Z Tian D Luo Q 《Molecular genetics and genomics : MGG》2007,277(1):31-42
Kernel number per spike is one of the most important yield components of wheat. To map QTLs related to kernel number including
spike length (SPL), spikelet number per spike (SPN), fertile spikelet number (FSPN), sterile spikelet number (SSPN) and compactness,
and to characterize the inheritance modes of the QTLs and two-locus interactions, 136 recombinant inbred lines (RILs) derived
from ‘Nanda2419’ x ‘Wangshuibai’ and an immortalized F2 population (IF2) generated by randomly permutated intermating of these RILs were investigated. QTL mapping made use of the previously constructed
over 3300 cM linkage map of the RIL population. Three, five, two, two and six chromosome regions were identified, respectively,
for their association with SPL, SPN, FSPN, SSPN, and compactness in at least two of the three environments examined. All compactness
QTLs but one shared the respective intervals of QSpn.nau-5A and the SPL QTLs. Xcfd46–Xwmc702 interval on chromosome 7D was related to all traits but SSPN and had consistently the largest effects. The fact that not
all the compactness QTL intervals were related to both SPL and SPN indicates that compactness is regulated by different mechanisms.
Interval coincidence between QTLs of SPL and SPN and between QTLs of FSPN and SSPN was minimal. For all the traits, favorable
alleles exist in both parents. Inheritance modes from additiveness to overdominance of the QTLs were revealed and two-locus
interactions were detected, implying that the traits studied are under complex genetic control. The results could contribute
to wheat yield improvement and better use of Wangshuibai and Nanda2419 the two special germplasms in wheat breeding program. 相似文献
16.
Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars 总被引:3,自引:0,他引:3
Quantitative trait loci (QTLs) with epistatic and QTL × environment (QE) interaction for heading date were studied using a
doubled haploid (DH) population containing 168 progeny lines derived from a cross between two elite Chinese wheat cultivars
Huapei 3 × Yumai 57 (Triticum aestivum L.). A genetic map was constructed based on 305 marker loci, consisting of 283 SSR loci and 22 EST-SSR markers, which covered
a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers in the genome. QTL analyses were
performed using a mixed linear model approach. Two main-effect QTLs and two pairs of digenic epistatic effects were detected
for heading date on chromosomes 1B, 2B, 5D, 6D, 7A, and 7D at three different environments in 2005 and 2006 cropping seasons.
A highly significant QTL with an F-value 148.96, designated as Qhd5D, was observed within the Xbarc320-Xwmc215 interval on chromosome 5DL, accounting for 53.19% of the phenotypic variance and reducing days-to-heading by 2.77 days. The
Qhd5D closely links with a PCR marker Xwmc215 with the genetic distance 2.1 cM, which can be used in molecular marker-assisted selection (MAS) in wheat breeding programs.
Moreover, the Qhd5D was located on the similar position of well-characterised vernalization sensitivity gene Vrn-D1. We are also spending more efforts to develop near-isogenic lines to finely map the Qhd5D and clone the gene Vrn-D1 through map-based cloning. The Qhd1B with additive effect on heading date has not been reported in previous linkage mapping studies, which might be a photoperiod-sensitive
gene homoeologous to the Ppd-H2 gene on chromosome 1B. No main-effect QTLs for heading date were involved in epistatic effects. 相似文献
17.
Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.) 总被引:13,自引:0,他引:13
Huang XQ Cloutier S Lycar L Radovanovic N Humphreys DG Noll JS Somers DJ Brown PD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(4):753-766
Development of high-yielding wheat varieties with good end-use quality has always been a major concern for wheat breeders. To genetically dissect quantitative trait loci (QTLs) for yield-related traits such as grain yield, plant height, maturity, lodging, test weight and thousand-grain weight, and for quality traits such as grain and flour protein content, gluten strength as evaluated by mixograph and SDS sedimentation volume, an F1-derived doubled haploid (DH) population of 185 individuals was developed from a cross between a Canadian wheat variety “AC Karma” and a breeding line 87E03-S2B1. A genetic map was constructed based on 167 marker loci, consisting of 160 microsatellite loci, three HMW glutenin subunit loci: Glu-A1, Glu-B1 and Glu-D1, and four STS-PCR markers. Data for investigated traits were collected from three to four environments in Manitoba, Canada. QTL analyses were performed using composite interval mapping. A total of 50 QTLs were detected, 24 for agronomic traits and 26 for quality-related traits. Many QTLs for correlated traits were mapped in the same genomic regions forming QTL clusters. The largest QTL clusters, consisting of up to nine QTLs, were found on chromosomes 1D and 4D. HMW glutenin subunits at Glu-1 loci had the largest effect on breadmaking quality; however, other genomic regions also contributed genetically to breadmaking quality. QTLs detected in the present study are compared with other QTL analyses in wheat. 相似文献
18.
G. Pressoir L. Albar N. Ahmadi I. Rimbault M. Lorieux D. Fargette A. Ghesquière 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1155-1161
The genetic basis of resistance to rice yellow mottle virus (RYMV) was studied in a doubled-haploid (DH) population derived
from a cross between the very susceptible indica variety ‘IR64’ and the resistant upland japonica variety Azucena. As a quantitative trait locus (QTL) involved in virus content estimated with an ELISA test has been previously
identified on chromosome 12, we performed a wide search for interactions between this QTL and the rest of the genome, and
between this QTL and morphological traits segregating in the population. Multiple regression with all identified genetic factors
was used to validate the interactions. Significant epistasis accounting for a major part of the total genetic variation was
observed. A complementary epistasis between the QTL located on chromosome 12 and a QTL located on chromosome 7 could be the
major genetic factor controlling the virus content. Resistance was also affected by a morphology-dependent mechanism since
tillering was interfering with the resistance mechanism conditioned by the epistasis between the two QTLs. Marker-assisted
backcross breeding was developed to introgress the QTLs of chromosome 7 and chromosome 12 in the susceptible ‘IR64’ genetic
background. First results confirmed that if both QTLs do not segregate in a backcross-derived F2 population, then the QTL of chromosome 12 cannot explain differences in virus content. A near-isogenic line (NIL) approach
is currently being developed to confirm the proposed genetic model of resistance to RYMV.
Received: 20 April 1990 / Accepted: 30 April 1998 相似文献
19.
QTL mapping for plant-height traits has not been hitherto reported in high-oil maize. A high-oil maize inbred ‘GY220’ was
crossed with two dent maize inbreds (‘8984’ and ‘8622’) to generate two connected F2:3 populations. Four plant-height traits were evaluated in 284 and 265 F2:3 families. Single-trait QTL mapping and multiple-trait joint QTL mapping was used to detect QTLs for the traits and the genetic
relationship between plant height (PH) and two other plant-height traits. A total of 28 QTLs and 12 pairs of digenic interactions
among detected QTLs for four traits were detected in the two F2:3 families. Only one marker was shared between the two populations. Joint analysis of PH with ear height (EH) and PH with top
height (TH) detected 32 additional QTLs. Our results showed that QTL detection for PH was dependent on the genetic background
of dent corn inbreds. Multiple-trait joint QTL analysis could increase the number of detected QTLs. 相似文献
20.
Chloupek O Forster BP Thomas WT 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):779-786
Root system size (RSS) was measured in 12 diverse barley genotypes and 157 double-haploid lines (DHs), using electric capacitance.
The parents of the DHs, Derkado and B83-12/21/5, carry different semi-dwarfing genes, sdw1 and ari-e.GP, respectively. Estimates of RSS were taken in the field thrice during plant development: stem elongation (RSS1), heading
(RSS2) and grain filling (RSS3). The 12 barley genotypes were assessed over 3 years and at two or three locations each year;
the DH mapping population was assessed at two locations in 2002. Among the 12 barley genotypes, those with the semi-dwarf
genes had greater RSS values in all 3 years (28.9, 24.6 and 15.0% in years 1, 2 and 3, respectively) compared to non-semi-dwarf
controls. The DH population showed transgressive segregation on both sides of the parent means, indicating polygenic control
of RSS. Quantitative trait loci (QTLs) for RSS were found on five of the seven chromosomes: 1H, 3H, 4H, 5H and 7H and these
were compared with previously mapped agronomic traits. The TotalRSS QTL on 3H was associated with sdw1 and QTLs for height, plant yield and plant weight. The RSS3 QTL on 5H was associated with ari-e.GP and QTLs for height, plant yield, plant weight, harvest index and tiller number. The RSS3 QTL on 7H was also associated
with a TotalRSS QTL and QTLs for plant weight and harvest index. Other RSS QTLs were not associated with any other trait studied.
RSS is considered to be a polygenic trait linked to important traits, in particular to yield. The study highlights the effects
of semi-dwarfing genes and discusses the potential for breeding for root traits. 相似文献