Components of partial resistance to Septoria nodorum in winter wheat

Components of partial resistance to Septoria nodorum were investigated in 10 cultivars of winter wheat having similar field resistances. The components measured were infection frequency, latent period, size, shape and rate of growth of lesions, spore production and its rate of increase. Latent period was found to be lognormally distributed. Some of the components of resistance were found to be significantly different between cultivars. Cluster analysis also showed that cultivars could be distinguished on the basis of their components of resistance. Principal components analysis indicated that resistance could be broken down into four underlying factors, three of which could be readily interpreted. The measurements of the components of resistance were combined in a model, the r-index, based on Van der Plank's r. The amount of variation between cultivars cast some doubt on the predictive value of the index but all the cultivar values were well within the range bounded by two ‘synthetic’ cultivars made up of combinations of either the most resistant or the most susceptible components. It is considered that the r-index has potential in screening for field resistance. The possibility of incorporating the most resistant-type components into one cultivar is discussed. The use of cultivar mixtures containing cultivars having similar field resistances is also discussed in the light of the variability found in this study.     

Genetic research as the valid base of strategies for breeding rust resistant wheats

It is known that few wheat cultivars maintain their resistance to rust diseases for a long period of time, particularly when crop populations become genetically more uniform. A number of genetically diverse, so far unexploited, sources of rust resistance in the natural as well as mutagenized population of wheat cultivars were identified. Several of these genes were placed in agronomically superior well-adapted backgrounds so that they could be used as pre-breeding stocks for introducing genetic diversity for resistance in a crop population. Some of these stocks when employed as parents in several cross combinations in a breeding programme have generated a number of promising cultivars with diversity for resistance.Many presently grown wheats in India, near-isogenic lines each with Lr14b, Lr14ab, Lr30 and certain international cultivars were identified as possessing diverse sources of adult plant resistance (APR) to leaf rust. Prolonged leaf rust resistance in some of the Indian cultivars was attributed to the likely presence of Lr34 either alone or in combination with other APR components. Tests of allelism carried out in certain cultivars that continue to show adequate levels of field resistance confirm the presence of Lr34, which explains the role that this gene has played in imparting durability for resistance to leaf rust. Also, Lr34 in combination with other APR components increases the levels of resistance, which suggests that combination of certain APR components should be another important strategy for breeding cultivars conferring durable and adequate levels of resistance. A new adult plant leaf rust resistance source that seems to be associated with durability in Arjun has been postulated. Likewise, cultivars possessing Sr2 in combination with certain other specific genes have maintained resistance to stem rust.Further, non-specific resistances that were transferred across widely different genotypes into two of the popular Indian wheats provided easily usable materials to the national breeding programmes for imparting durable resistance to stripe rust.     

Assessing cultivar resistance to Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae) using a phenotyping method under semi‐field conditions

The orange wheat blossom midge, Sitodiplosis mosellana (Géhin), can significantly reduce wheat yield. Growing resistant wheat cultivars is an effective way of managing this pest. The assessment of cultivar resistance in field trials is difficult because of unequal pressure of S. mosellana caused by differences in cultivar heading dates relative to the flight period of S. mosellana adult females and huge variations of egg laying conditions from 1 day to another. To overcome these hurdles and to expose all cultivars homogeneously to the pest, an assessment method of cultivar resistance was developed under semi‐field conditions. In 2015, the resistance of 64 winter wheat cultivars to S. mosellana was assessed. Few or no larvae developed in the ears of resistant cultivars, but in susceptible cultivars, large numbers of larvae developed. Seventeen cultivars proved to be resistant, whereas 47 were susceptible. The identification of new resistant cultivars offers more opportunities to manage S. mosellana. The phenotyping method is easy, cheap, efficient and reliable. It can be used to guide the breeding of new resistant wheat cultivars. Using specific midge populations, this method could also be used in research on new resistance mechanisms in winter wheat or in other cereal species.     

Components of partial resistance as criteria for identifying resistance

The components of partial resistance, incubation period, lesion area, latent period and sporulation were recorded on plants of six winter and two spring wheat cultivars which had been artificially inoculated with Septoria nodorum spores. Incubation period gave a guide as to how the cultivars would respond in the field to Septoria nodorum but statistical analysis showed that it could not be used alone to predict accurately the resistance of each cultivar to the pathogen. Average sporulation, however, could be used with more confidence for predicting the field resistance of the cultivars. From a regression analysis of NIAB rating versus incubation period, lesion area, latent period and sporulation, an equation was devised to obtain resistance indices for each cultivar. These resistance indices clearly reflected the NIAB ratings for the cultivars. It would therefore appear that resistance indices could be used as a pre-field evaluation method for identifying resistance to Septoria nodorum and thus be a valuable technique in breeding programmes.     

Effect of yellow rust on yield components of barley cultivars with race-specific and slow rusting resistance to yellow rust

Yellow rust caused by Puccinia striiformis f. sp. hordei is an important disease of barley (Hordeum vulgare L.) in some parts of the world. We compared the effectiveness of different types of resistance in field plots at Ardabil Agricultural Research Station (Iran) during 2010–2011. Yield components along with slow rusting parameters including final rust severity (FRS), apparent infection rate (r), relative area under disease progress curve (rAUDPC) and coefficient of infection (CI) were evaluated for 25 barley cultivars. In all, two barley cultivars with race-specific resistance, 19 cultivars with different levels of slow rusting resistance and four susceptible cultivars were included in two experiments with and without fungicide protection under high disease pressure. Barley cultivars with slow rusting resistance displayed a range of severity responses indicating phenotypic diversity. Mean thousand kernels weight (TKW) losses for susceptible, race-specific and slow rusting genotypes were 31, 3 and 12%, respectively, and mean kernels per spike (KPS) losses for susceptible, race-specific and slow rusting genotypes were 19, 0.2 and 8%, respectively. Correlation coefficient of mean TKW and KPS losses with epidemiological parameters; rAUDPC, r, CI and FRS were highly significant. Slow rusting cultivars with low values of different parameters as well as genotypes with low yield component losses despite moderate disease levels were identified. Such genotypes can be used for breeding barely genotypes with high levels of resistance and negligible yield losses.     

The management of potato cyst nematodes using resistant Solanaceae potato clones as trap crops

The concept of using a range of Solanaceae potato clones as trap crops for potato cyst nematode (PCN) management was investigated. A series of field trials were undertaken from 1999 to 2002 that evaluated 10 clones of either wild Solanum potato species, breeder’s hybrid lines or commercial cultivars. All had high resistance to all known PCN pathotypes (both Globodera rostochiensis and Globodera pallida) and the ability to stimulate high levels of PCN hatch. Investigations showed potential for the development of some clones as a means of reducing high PCN field population levels and for use by organic potato producers.     

Screening for resistance in apple cultivars to lightbrown apple moth, Epiphyas postvittana, and greenheaded leafroller, Planotortrix octo, and its relationship to field damage

The mature leaves of 38 apple (Malus domestica Borkh.) (Rosaceae) cultivars were screened for resistance to laboratory colonies of Epiphyas postvittana (Walker) and Planotortrix octo Dugdale (Lepidoptera: Tortricidae) by measuring larval survival and development rate, and pupal weight, in no‐choice laboratory bioassays in early summer. There were few cultivar effects on larval mortality but Nevis 1, A40R04T119, and ‘Sir Prize’ reduced survival of E. postvittana. Effects on development time and weight were correlated, and were integrated into a single measure of resistance R_c. R_c varied greatly between cultivars for both leafroller species, and identified 15 and 11 cultivars with partial resistance to E. postvittana and P. octo, respectively. A further experiment with three of the cultivars in midsummer, using the laboratory colonies in comparison with new colonies (from field collected larvae), produced similar results for E. postvittana, but there was no survival of new colony P. octo larvae on two cultivars. These results for P. octo are consistent with other recent research showing extreme cultivar resistance and critical colony and seasonal influences. A series of 4‐year field trials with the 38 cultivars showed poor correlation between laboratory and field resistance for the dominant leafroller species in the field, E. postvittana. The combined data, however, identified ‘Red Dougherty’, ‘Sir Prize’, and A40R04T119 as potentially useful in breeding for resistance to this species. The implications of these findings for integrated pest management (IPM) programmes and for the breeding of leafroller‐resistant apple cultivars are discussed.     

Fiji disease resistance in sugarcane: Relationship to cultivar preference in field populations of the planthopper vector Perkinsiella saccharicida

Populations of the planthopper vector Perkinsiella saccharicida on sugarcane cultivars resistant (cvs QUO and Q87), moderately resistant (cvs Q90 and Q124) and susceptible (cvs NCo310 and Q102) to Fiji disease with known field resistance scores were monitored on the plant (2000–2001) and ratoon (2001–2002) crops. In both crops, the vector population remained very low, reaching its peak in the autumn. The vector population was significantly higher on cultivars susceptible to Fiji disease than on cultivars moderately resistant and resistant to Fiji disease. The number of P. saccharicida adults, nymphs and oviposition sites per plant increased with the increase in the Fiji disease susceptibility. The results suggest that under low vector density, cultivar preference by the planthopper vector mediates Fiji disease resistance in sugarcane. To obtain resistance ratings in the glasshouse that reflect field resistance, glasshouse‐screening trials should be conducted under both low and high vector densities, and the cultivar preference of the planthopper vector recorded along with Fiji disease incidence.     

Sorghum root length density and the potential for avoiding striga parasitism

Striga hermonthica is a serious root parasite of sorghum in the semiarid tropics. Successful parasitism is dependent on interactions of Striga seeds and host roots. Several sorghum cultivars have been found which resist parasitism. The basis of resistance is not well known. One possible method for reducing the chances of parasitism is by restricted host root development. This research was conducted to evaluate this hypothesis in sorghum known to possess resistance to parasitism by Striga.Root length density of 21-day-old pot-grown resistant cultivars, Framida, N-13, IS-9830, Tetron and P-967083, were compared to that of the susceptible check, Dabar, using the line intercept method of measuring root length. There was no significant difference between resistant cultivars and the susceptible cultivar Dabar. The RLD of resistant P-967083 however was significantly less than Framida, another resistant cultivar.The RLD of Dabar was compared to that of Framida and P-967083 in USA and Niger field trials. Root length density was determined on soil cores taken at flowering with a Giddings Soil Sampler. Each core was divided into 10-cm fractions for estimating RLD by the line intercept method. In the USA Dabar had significantly greater RLD than the two resistant cultivars in the upper 10-cm portion of the soil profile, but only significantly greater than P-967083 in the 10–20-cm portion. Significant differences in RLD between susceptible and resistant cultivars were not found at depths between 20–60 cm. In field trials in Niger, RLD of Dabar was significantly greater than either resistant cultivar in the (0 to 30 cm) portion of the soil core. These results suggest that part of the Striga resistance of P-967083 and perhaps Framida may be a result of avoiding interactions between parasitic seeds and host roots.     

Effects of cultivar and fungicides on stem-base pathogens, determined by quantitative PCR, and on diseases and yield of wheat

The polymerase chain reaction (PCR) was used to identify and quantify all fungal pathogens of wheat (Triticum aestivum) stem bases in nine field experiments at three locations in England. The main aim was to apply quantitative PCR to provide robust data on the efficacy of new fungicides against the individual components of the stem‐base disease complex. Cyprodinil most effectively controlled eyespot by decreasing both pathogens, Tapesia yallundae and T. acuformis (the most widespread species), and sometimes contributed to increased yields. Prochloraz controlled eyespot less consistently, its effectiveness dependent mainly on the presence of T. yallundae or on rainfall events soon after application. Azoxystrobin contributed to yield increases most consistently. Although it decreased sharp eyespot and its pathogen, Rhizoctonia cerealis, these effects were insufficient to account for much of the yield increases. The effects of fungicides on eyespot were sometimes greatest on the most susceptible cultivars. Amounts of Tapesia DNA were usually consistent with cultivar susceptibility ratings. The only pathogens of brown foot rot present in significant amounts were Microdochium nivale vars nivale and majus. They appeared not to affect yield or to respond greatly to fungicides. The susceptibility of cultivars to these pathogens was sometimes similar to their susceptibility to eyespot, suggesting that they may respond to the same host resistance genes or may in some cases be secondary colonisers of eyespot‐infected plants.     

Components of partial resistance of barley to Rhynchosporium secalis: use of seedling tests to predict field resistance

Components of partial resistance [disease incidence (DI), infection frequency (IF), latent period (LP), spores per lesion (SPL)] were assessed on glasshouse-grown barley seedlings following inoculation with spore suspensions of Rhynchosporium secalis at growth stage 12 (Zadoks, Chang & Konzak, 1974). Four experiments were carried out at different times during 1988. Three spring barley cultivars [two from Cyprus (cvs Kantara and Athenais) and one from the UK (cv. Triumph)] were used in the first three experiments. In the fourth experiment eight additional UK cultivars with NIAB resistance ratings ranging from 3 to 9 were used. Two races of R. secalis were used in the first three experiments and three in the fourth. The three cultivars, Kantara, Athenais and Triumph, were examined in all four experiments and significant differences detected for virtually all components of partial resistance in each. Differences, however, were often small and ranking of cultivars varied in different experiments. The greater susceptibility of cv. Kantara compared to cv. Athenais, observed under field conditions in Cyprus, would not be anticipated from the small differences in components of partial resistance observed in these experiments, but, for these cultivars, the possibility of a marked genotype x environment interaction cannot be discounted. Mean values for the components of partial resistance differed in the four experiments. LP was correlated with mean glasshouse temperature from inoculation to the onset of sporulation but differences in IF and SPL were not correlated with temperature. For these components, light quality and/or duration appeared to be more important. Overall, there were no differences between races but significant race X cultivar interactions were observed in two experiments. In the fourth experiment, examining 11 cultivars, there were significant differences between cultivars for all components of partial resistance. IF and LP were correlated but neither of these components was correlated with SPL indicating independent control of this latter component. Both IF and LP were correlated with field performance (NIAB ratings) but there was no correlation with SPL. However, combining IF with mean values of SPL restricted to the 5 days following the end of the LP, produced the best correlation (r= 0.92) with NIAB ratings. Problems of assessing components of partial resistance and possible means of improving assessments are discussed.     

Control of potato cyst nematode (Globodera pallida) by host plant resistance and nematicide

Seven trials conducted over four years on sites naturally infested with the white potato cyst nematode established that potato clones bred for resistance to Globodera pallida allowed significantly less nematode multiplication than conventional cultivars under field conditions. Nematode multiplication was inversely related to initial infestation level. The nematicide, aldicarb, significantly reduced nematode multiplication. However, nematode multiplication on nematicide treated susceptible cultivars was greater than on untreated partially resistant clones, indicating that resistance may offer more effective control of G. pallida than chemical treatment. Integration of host plant resistance and nematicide treatment is discussed.     

Effect of the nematicide oxamyl on the multiplication of Globodera rostochiensis and G. pallida and on the haulm growth and yield of six potato cultivars with different levels of resistance and tolerance

The effect of a pre-planting application of oxamyl on the yields of six potato cultivars was studied in co-operative field trials in 1981. Two sites were ‘uninfested’ with potato cyst nematodes (PCN), two were lightly infested (<25 eggs/g soil) and six were moderately to heavily infested (three with Globodera rostochiensis and three with G. pallida). At the uninfested and lightly infested sites oxamyl had little effect on mid-season haulm weights or on final tuber yields. At sites moderately to heavily infested with G. rostochiensis the haulm growth of all cultivars tended to be increased by a similar amount on plots treated with oxamyl, Pentland Dell being least responsive. Yield was increased by different amounts, the increases being least for cvs Cara and Maris Piper and most for Corsair and Pentland Dell. At the sites moderately to heavily infested with G. pallida Cara was again tolerant, its yield being increased little by oxamyl compared with the other cultivars. Maris Piper gave the largest yield increase. Final populations of PCN on non-resistant cultivars were reduced by oxamyl at some sites but not at others. Resistant cultivars also decreased the final numbers of PCN at most sites. Two cultivars derived from Solanum vernei with different degrees of resistance, appeared to be almost equally effective in controlling G. rostochiensis and G. pallida.     

Differences in the tolerance of potato cultivars to potato cyst nematodes (Globodera rostochiensis and G. pallida) in field trials with and without nematicides

In field trials Cara, Brio, Maris Piper and Pentland Javelin were consistently more tolerant of damage by Globodera rostochiensis yielding more than Corsair, Pentland Dell, Maris Anchor and Maris Peer, in untreated, heavily infested soil and giving the smallest increases to nematicide treatment. No yield or growth differences were found between plants in untreated and nematicide treated plots at a nematode-free site. The most tolerant cultivars all had a gene (H₁) for resistance to G. rostochiensis derived from Solanum tuberosum ssp. andigena and in soil infested with G. pallida the tolerance of at least one resistant cultivar (Maris Piper) appeared to be lessened. However, some resistant cultivars were comparatively intolerant, even to G. rostochiensis. Early cultivars were generally less tolerant than late maturing cultivars but there were exceptions. Amongst cultivars with resistance derived from Solanum vernei the early cultivar Guardian was more tolerant than the main crop cultivar Corsair. The effect on the yield of several cultivars of a range of densities of G. rostochiensis, produced either by applying different rates of a nematicide or by cropping in the previous year, was examined at two sites. The results indicated that the slope of the regression for yield in relation to nematode density was less for tolerant than intolerant cultivars. At sites infested with G. rostochiensis Maris Piper was found to be consistently more tolerant than Pentland Crown.     

An Association Between High Peroxidase Activity in Lettuce (Lactuca sativa) and Field Resistance to Downy Mildew (Bremia lactucae)

The association between variation for pre-infection peroxidase activity and levels of field resistance-susceptibility to downy mildew (Bremia lactucae) was investigated in lettuce (Lactuca sativa) cultivars, accessions of L. serriola (prickly lettuce), segregating F₂ populations and selected F₃ families from a cross between field resistant and susceptible lettuce cultivars. A trend was apparent in this series of experiments indicating that one component of field resistance could be related to a high level of peroxidase activity prior to infection. The data suggest that in breeding programmes there could be merit in imposing primary selection for high peroxidase activity prior to field selection for resistance.     

Long-term impacts of elevated carbon dioxide and transgenic Bt cotton on performance and feeding of three generations of cotton bollworm

A field screening trial undertaken in Uganda showed that a number of Musa L. (Musaceae) cultivars and hybrids displayed high levels of resistance to banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), while most highland banana cultivars tended to be susceptible to weevil attack. In this study, research was undertaken to determine modalities of resistance to banana weevil. Laboratory studies suggested that all cultivars were attractive to the weevil and that females freely oviposited on all cultivars. While some differences were found between cultivars in attractivity and egg numbers, these were not related to subsequent damage. Therefore, antixenosis does not appear to play an important role in host‐plant resistance to banana weevil. Larval survivorship rates in living corms were, for the most part, low on resistant cultivars, suggesting that antibiosis mechanisms offer the primary avenues of resistance. In the laboratory, development was slower on some resistant cultivars although survivorship rates on excised corm material were not as well related to levels of resistance as that on living material. Sap appeared to play a minor role in reducing egg eclosion rates on some resistant cultivars. Methanol extracts from Kayinja, a resistant cultivar, inhibited larval development on corms of susceptible cultivars in the laboratory.     

The Expression of Partial Resistance of Wheat to Puccinia recondita

A range of winter wheat cultivars, which appeared to show no race-specific resistance to Puccinia recondita f. sp. tritici, was studied at seedling and adult growth stages to determine the components of partial resistance which might contribute to slow-rusting in the field. Under controlled environmental conditions, adult plants of cvs Atou, Bouquet, Cappelle-Desprez and Maris Freeman produced less uredospores per unit area than the control cv. Armada, and uredosori were smaller on these cultivars. Fewer uredosori erupted on cv. Cappelle-Desprez than on the control, and both cv. Bouquet and cv. Cappelle-Desprez showed longer latent periods than other cultivars. Cultivar Holdfast had a shorter latent period, with larger and more numerous uredosori than even the highly susceptible cv. Armada. Cultivar Waggoner displayed a similar response to infection to that of the control. The components of partial resistance detected in adult plants were not displayed by seedling leaves.     

Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars

Interspecific somatic hybrids between commercial cultivars of potato Solanum tuberosum L. Agave and Delikat and the wild diploid species Solanum cardiophyllum Lindl. (cph) were produced by protoplast electrofusion. The hybrid nature of the regenerated plants was confirmed by flow cytometry, simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP), microsatellite-anchored fragment length polymorphism (MFLP) markers and morphological analysis. Somatic hybrids were assessed for their resistance to Colorado potato beetle (CPB) using a laboratory bioassay, to Potato virus Y (PVY) by mechanical inoculation and field trials, and foliage blight in a greenhouse and by field trials. Twenty-four and 26 somatic hybrids of cph + cv. Agave or cph + cv. Delikat, respectively, showed no symptoms of infection with PVY, of which 3 and 12, respectively, were also resistant to foliage blight. One hybrid of cph + Agave performed best in CPB and PVY resistance tests. Of the somatic hybrids that were evaluated for their morphology and tuber yield in the field for 3 years, four did not differ significantly in tuber yield from the parental and standard cultivars. Progeny of hybrids was obtained by pollinating them with pollen from a cultivar, selfing or cross-pollination. The results confirm that protoplast electrofusion can be used to transfer the CPB, PVY and late blight resistance of cph into somatic hybrids. These resistant somatic hybrids can be used in pre-breeding studies, molecular characterization and for increasing the genetic diversity available for potato breeding by marker-assisted combinatorial introgression into the potato gene pool.     

Development of Pseudocercosporella herpotrichoides (Fron) Deighton var. herpotrichoides and var. acuformis on Wheat Plants Measured by ELISA

Using the ELISA method, the development of Pseudocercosporella herpotrtchoides var. herpotrichoides and var. acuformis in a susceptible cultivar of winter wheat was compared under controlled and held conditions. In the greenhouse, var. acufornis grew less vigorously, was slower in penetrating the coleoptile and the successive leaf sheaths and in colonizing the stem tissue than var. herpotrichoides. In the field, these differences were confirmed on the last leaf sheaths and the stem. At ripening stage, however, identical ELISA values were measured for both varieties. Moreover, a significant variation was observed between the individual isolates of each variety. Comparison of the effect of both varieties of P. herpotrichoides on 20 wheat cultivars characterized by different resistance levels showed significant interactions. The cultivars carrying the Pch-1 gene always remained the less diseased genotypes. In general, var. acuformis developed less antigen in the cultivars than var. herpotrichoides. It is concluded, that in tests for resistance to P. herpotrichoides mixtures of many highly pathogenic isolates of both fungus varieties should be used. Less complex mixtures or single isolates may result in wrong estimates of resistance.