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1.
Chlorophyll a fluorescence and carbon assimilation in developing leaves of light-grown cucumber 总被引:1,自引:0,他引:1
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The development of photochemical activity and carbon assimilation in light-grown cucumber (Cucumis sativus L. cv Natsusairaku) leaves was studied to determine the pattern of acquisition and its relationship to leaf growth and expansion. Measurements of chlorophyll a fluorescence showed that leaves acquire photochemical function over a period of 6 or more days, and gas exchange studies showed increases in carbon assimilation over a parallel time period. As leaves expand and mature, they undergo a sequential, three-step series of changes in fluorescence response. The initial kinetics show the absence of wholly functional quenching mechanisms. Dynamic imaging of fluorescence kinetics showed that a temporal series of changes occurred within defined areas of individual developing leaves. The spatial acquisition of photochemical activity in leaves was basipetal as is their directional expansion, development of air spaces and stomata, and the cessation of imported carbon. 相似文献
2.
Photosynthesis in localised regions of oat leaves infected with crown rust (Puccinia coronata): quantitative imaging of chlorophyll fluorescence 总被引:1,自引:0,他引:1
Localised changes in photosynthesis in oat leaves infected with the biotrophic rust fungus Puccinia coronata Corda were examined at different stages of disease development by quantitative imaging of chlorophyll fluorescence. Following inoculation of oat leaves with crown rust the rate of whole-leaf gas exchange declined. However, crown rust formed discrete areas of infection which expanded as the disease progressed and these localised regions of infection gave rise to heterogeneous changes in photosynthesis. To quantify these changes, images of chlorophyll fluorescence were taken 5, 8 and 11 d after inoculation and used to calculate images representing two parameters; ΦII, a measure of PSII photochemical efficiency and ΔFm/Fm′, a measure of non-photochemical energy dissipation (qN). Five days after inoculation, disease symptoms appeared as yellow flecks which were correlated with the extent of the fungal mycelium within the leaf. At this stage, ΔII was slightly reduced in the infected regions but, in uninfected regions of the leaf, values of ΦII were similar to those of healthy leaves. In contrast, qN (ΔFm/Fm′) was greatly reduced throughout the infected leaf in comparison to healthy leaves. We suggest that the low value of qN in an infected leaf reflects a high demand for ATP within these leaves. At sporulation, 8 d after inoculation, ΦII was reduced throughout the infected leaf although the reduction was most marked in areas invaded by fungal mycelium. In the infected leaf the pattern of non-photochemical quenching was complex; qN was low within invaded regions, perhaps reflecting high metabolic activity, but was now much higher in uninfected regions of the infected leaf, in comparison to healthy leaves. Eleven days after inoculation “green islands” formed in regions of the leaf associated with the fungal mycelium. At this stage, photosynthesis was severely inhibited over the entire leaf; however, heterogeneity was still apparent. In the region not invaded by the fungal mycelium, ΦII and qN were very low and these regions of the leaf were highly fluorescent, indicating that the photosynthetic apparatus was severely damaged. In the greenisland tissue, ΦII was low but detectable, indicating that some photosynthetic processes were still occurring. Moreover, qN was high and fluorescence low, indicating that the cells in this region were not dead and were capable of significant quenching of chlorophyll fluorescence. 相似文献
3.
Transitions in photosynthetic parameters of midvein and interveinal regions of leaves and their importance during leaf growth and development 总被引:1,自引:0,他引:1
Abstract: The areal development of photosynthetic efficiency and growth patterns in expanding leaves of two different dicotyledonous species - Coccoloba uvifera and Sanchezia nobilis - was investigated by imaging both processes repeatedly over 32 days. Measurements were performed using combined imaging systems for chlorophyll fluorescence and growth, with the same spatial resolution. Significant differences in potential quantum yield of photosynthesis (Fv /Fm ), a parameter indicating the functional status of photosystem II, were found between midvein and interveinal tissue. Although base-tip gradients and spatial patchiness were observed in the distribution of relative growth rate, neither midvein nor interveinal tissue showed such patterns in Fv /Fm . In young leaves, Fv /Fm of the midvein was higher than Fv /Fm of interveinal tissue. This difference declined gradually with time, and upon cessation of growth, Fv /Fm of interveinal regions exceeded those of midvein tissue. Images of chlorophyll fluorescence quenching showed that ΔF/Fm ' in the different tissues correlated with Fv /Fm , indicating that, in these uniformly illuminated leaves, transitions in photosynthetic electron transport activity follow those of predawn quantum efficiency. We explore the implications of these observations during leaf development, discuss effects of sucrose delivery from veins to interveinal areas on relative rates of photosynthetic development in these tissues, and propose that the initially higher photosynthetic activity in the midvein compared to the intervein tissues may supply carbohydrates and energy for leaf growth processes. 相似文献
4.
5.
Janosch Sedlacek Julia A. Wheeler Andrés J. Cortés Oliver Bossdorf Guenter Hoch Christian Lexer Sonja Wipf Sophie Karrenberg Mark van Kleunen Christian Rixen 《PloS one》2015,10(4)
Climate change is altering spring snowmelt patterns in alpine and arctic ecosystems, and these changes may alter plant phenology, growth and reproduction. To predict how alpine plants respond to shifts in snowmelt timing, we need to understand trait plasticity, its effects on growth and reproduction, and the degree to which plants experience a home-site advantage. We tested how the common, long-lived dwarf shrub Salix herbacea responded to changing spring snowmelt time by reciprocally transplanting turfs of S. herbacea between early-exposure ridge and late-exposure snowbed microhabitats. After the transplant, we monitored phenological, morphological and fitness traits, as well as leaf damage, during two growing seasons. Salix herbacea leafed out earlier, but had a longer development time and produced smaller leaves on ridges relative to snowbeds. Longer phenological development times and smaller leaves were associated with reduced sexual reproduction on ridges. On snowbeds, larger leaves and intermediate development times were associated with increased clonal reproduction. Clonal and sexual reproduction showed no response to altered snowmelt time. We found no home-site advantage in terms of sexual and clonal reproduction. Leaf damage probability depended on snowmelt and thus exposure period, but had no short-term effect on fitness traits. We conclude that the studied populations of S. herbacea can respond to shifts in snowmelt by plastic changes in phenology and leaf size, while maintaining levels of clonal and sexual reproduction. The lack of a home-site advantage suggests that S. herbacea may not be adapted to different microhabitats. The studied populations are thus unlikely to react to climate change by rapid adaptation, but their responses will also not be constrained by small-scale local adaptation. In the short term, snowbed plants may persist due to high stem densities. However, in the long term, reduction in leaf size and flowering, a longer phenological development time and increased exposure to damage may decrease overall performance of S. herbacea under earlier snowmelt. 相似文献
6.
Growth and Deposition of Inorganic Nutrient Elements in Developing Leaves of Zea mays L 总被引:3,自引:2,他引:1
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Spatial distributions of growth and of the concentration of some inorganic nutrient elements were analyzed in developing leaves of maize (Zea mays L.). Growth was analyzed by pinprick experiments with numerical analysis to characterize fields of velocity and relative elemental elongation rate. Inductively coupled plasma and atomic emission spectroscopy were used to measure nutrients extracted from segments of leaf tissue collected by position. Leaves 7 and 8, both elongating 3 millimeters per hour had maximum relative elemental growth rates of 0.06 to 0.08 millimeters per hour with maximum rates 20 to 50 millimeters from the node and cessation of growth by 90 millimeters from the node. Spatial distribution of dry weight density revealed that the rate of biomass deposition was maximum in the most rapidly expanding region and continued beyond the elongation zone. The nutrient elements K, Cl, Ca, Mg, and P showed different distribution patterns of ion density (on a dry weight basis). K and Cl had minimal density in the leaf tips; K density was maximum in the growing region, whereas Cl density was maximum at the region of growth cessation. Ca, Mg, and P had relatively high densities at the base of the elongation zone near the node and also in the tip regions. Near the node, P and Mg densities were higher in the young, growing leaves, whereas Ca density near the node was higher in older leaves that had completed elongation. Deposition rates of all nutrients were greatest in the region of maximum elongation rate. 相似文献
7.
Chloroplast Structure and Function Is Altered in the NCS2 Maize Mitochondrial Mutant 总被引:11,自引:2,他引:9
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The nonchromosomal stripe 2 (NCS2) mutant of maize (Zea mays L.) has a DNA rearrangement in the mitochondrial genome that segregates with the abnormal growth phenotype. Yet, the NCS2 characteristic phenotype includes striped sectors of pale-green tissue on the leaves. This suggests a chloroplast abnormality. To characterize the chloroplasts present in the mutant sectors, we examined the chloroplast structure by electron microscopy, chloroplast function by radiolabeled carbon dioxide fixation and fluorescence induction kinetics, and thylakoid protein composition by polyacrylamide gel electrophoresis. The data from these analyses suggest abnormal or prematurely arrested chloroplast development. Deleterious effects of the NCS2 mutant mitochondria upon the cells of the leaf include structural and functional alterations in the both the bundle sheath and mesophyll chloroplasts. 相似文献
8.
Metallothioneins 1 and 2 Have Distinct but Overlapping Expression
Patterns in Arabidopsis 总被引:12,自引:0,他引:12
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The spatial and temporal expression patterns of metallothionein (MT) isoforms MT1a and MT2a were investigated in vegetative and reproductive tissues of untreated and copper-treated Arabidopsis by in situ hybridization and by northern blotting. In control plants, MT1a mRNA was localized in leaf trichomes and in the vascular tissue in leaves, roots, flowers, and germinating embryos. In copper-treated plants, MT1a expression was also observed in the leaf mesophyll and in vascular tissue of developing siliques and seeds. In contrast, MT2a was expressed primarily in the trichomes of both untreated and copper-treated plants. In copper-treated plants, MT2a mRNA was also expressed in siliques. Northern-hybridization studies performed on developing seedlings and leaves showed temporal variations of MT1a gene expression but not of MT2a expression. The possible implications of these findings for the cellular roles of MTs in plants are discussed. 相似文献
9.
《Aquatic Botany》2005,81(1):69-84
We used the Imaging-PAM fluorometer to map spatial variability of photosynthesis in three seagrass species, Halophila ovalis, Zostera capricorni and Posidonia australis. Photosynthesis was described by relative photosynthetic rate (PS/50), effective quantum yield (ΦPSII), non-photochemical quenching (NPQ and qN), electron transport rate (ETR) and leaf absorptivity. Photosynthetic patterns were linked to leaf age and light climate but patterns were not consistent across species. Longitudinal heterogeneity in photosynthesis was apparent along the leaves of all three species while lateral spatial heterogeneity was found only across Z. capricorni and H. ovalis leaves. Age of leaf tissue, determined by longitudinal location on the leaf, strongly influenced photosynthetic activity of Z. capricorni and P. australis. A comparison of H. ovalis leaves of differing maturity demonstrated the influence of leaf age on photosynthetic activity, yet a comparison of Z. capricorni leaves of differing maturity showed no leaf-age effects.Variations in stress-induced changes across a seagrass leaf can be used to identify areas or particular regions of the leaf, which are more susceptible to photodamage. Clear evidence of substantial within-leaf heterogeneity in photosynthetic activity (i.e., a two-fold variation in half saturation constant along a leaf of P. australis) has serious implications for use of small sections of leaf for photosynthetic incubations (such as O2 or single-point chlorophyll a fluorescence measurements). 相似文献
10.
Philip R. Larson 《American journal of botany》1986,73(11):1620-1631
The odd-pinnate leaves of Polyscias quilfoylei have a sheathing leaf base that completely encircles the stem. At each node, many traces depart the vascular cylinder and traverse an obliquely upward course through the leaf base before aggregating in the rachis. Lateral traces diverge from parent traces in the stem vasculature at variable times relative to the leaf they serve, from variable positions in the vascular cylinder and from parent traces of variable ages. The stem vasculature is formed by the coalescing of leaf traces from as many as five leaves. All bundles departing the vascular cylinder at a node to serve a leaf are true leaf traces originating independently in the stem. Leaf traces develop acropetally from their positions of origin on parent traces. Primordial leaves are first served by the median trace and later by lateral traces. Many traces were recognized in the internodes subtending embryonic leaves, but they could not be related either to a specific leaf or to a specific position within a leaf. Because these traces had not yet achieved contact with a primordial leaf site, they were assumed to be in the process of developing acropetally at the time of sampling. Observations suggest that the multiple traces in this species might perform a similar function of integrating the vascular cylinder that subsidiary bundles perform in certain uni- and trilacunar species. 相似文献
11.
Background and Aims
Epidermal phenolic compounds (mainly flavonoids) constitute a vital screen that protects the leaf from damage by natural ultraviolet (UV) radiation. The effectiveness of epidermal UV-screening depends on leaf anatomy, the content of UV-screening compounds and their spatial uniformity over the leaf area. To investigate in vivo the spatial pattern of the epidermal UV-screen during leaf development, a fluorescence imaging method was developed to map the epidermal UV-absorbance at a microscopic scale. This study was done on oak (Quercus petraea) leaves that were used as a model of woody dicotyledonous leaves.Methods
The leaf development of 2-year-old trees, grown outdoors, was monitored, at a macroscopic scale, by in vivo measurements of chlorophyll content per unit area and epidermal UV-absorbance using two optical leaf-clip meters. The distribution of pigments within leaves was assessed in vivo spectroscopically. The microscopic images of UV-induced fluorescence and UV-absorbance acquired in vivo during leaf development were interpreted from spectral characteristics of leaves.Key Results
At a macroscopic scale, epidermal UV-absorbance was high on the upper leaf side during leaf development, while it increased on the lower leaf side during leaf expansion and reached the adaxial value at maturity. At a microscopic scale, in immature leaves, for both leaf sides, the spatial distribution of epidermal UV-absorbance was heterogeneous, with a pattern depending on the flavonoid content of vacuoles in developing epidermal cells. At maturity, epidermal UV-absorbance was uniform.Conclusions
The spatial pattern of epidermal UV-screen over the area of oak leaves is related to leaf anatomy during development. In vivo spectroscopy and fluorescence imaging of the leaf surface showed the distribution of pigments within the leaf and hence can provide a tool to monitor optically the leaf development in nature.Key words: Blue-green fluorescence, chlorophyll fluorescence, epidermis, flavonoids, leaf development, microscopic imaging, polyphenols, Quercus petraea 相似文献12.
《Flora》2007,202(4):261-267
Although stomatal ontogeny is closely related to the development and maturation of the epidermal tissue, stomatal patterns in relation to cuticle construction and cuticular material deposition during leaf development have not received adequate attention. We observed the deposition of blue-fluorescing cuticular phenolics over guard and epidermal cells, as well as stomatal formation and patterning using the alkali-induced blue fluorescence of the cuticle of Prunus persica leaves. Stomata of different stages of maturity occurred together during leaf development, mainly at the tip of the lamina. The deposition of fluorescing compounds initially appeared over the guard cells of the developing stomata complexes and gradually extended to the neighbouring epidermal cells. Based on the blue fluorescence emitted by the cuticular layers, we constructed digital maps of leaves of different developmental stages, showing the pattern of stomatal formation and deposition of fluorescing compounds. A longitudinal tip-to-base gradient in the formation of stomata, as well as in the deposition of fluorescing compounds was observed in young developing leaves. The deposition of blue-fluorescing phenolic compounds seems to be coordinated with stomatal development. 相似文献
13.
《Acta Oecologica》2000,21(1):63-77
Seasonal dynamics of biomass and nitrogen allocation in ramets of the clonal perennial Solidago altissima in response to yearly mowing were assessed in a field experiment. Final biomass of all component organs of the ramets was lower in mown than in unmown plots. Except for a negative effect on rhizome nitrogen concentration, mowing did not influence tissue nitrogen concentrations but, as a consequence of the reduced biomass accumulation, pools of standing-crop nitrogen were reduced in all organs. At all times during the annual stand development, the nitrogen concentration in the leaf canopies declined exponentially from the top downwards. The gradient of this decline was most marked at the beginning of the season and then became less apparent. In the mature stands, the area-based nitrogen contents of `upper-canopy' leaves (receiving > 50 % of incoming light), for a given light availability, on average were greater in mown than in unmown plots. About half of the nitrogen still remaining in pre-senescent leaves sampled in July was re-absorbed before leaf death. Nevertheless, the decreases in the absolute leaf- and stem-nitrogen pools per ramet during reproductive growth were much lower than the concomitant nitrogen gains of the developing inflorescences, implying that more than half of the nitrogen allocated to reproductive structures was taken up from the soil. On the other hand, there was evidence for nitrogen re-allocation from old to new leaves during vegetative growth which apparently enabled individual ramets to maintain an exponential nitrogen profile even during the phase of rapid leaf production in spring, thus using nitrogen efficiently. 相似文献
14.
Response of leaf ontogeny and photosynthetic activity to reproductive growth in cotton 总被引:2,自引:0,他引:2
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Wells R 《Plant physiology》1988,87(1):274-279
This study was conducted to determine if reproductive growth in cotton (Gossypium hirsutum L.) affects concurrent leaf development. Apparent photosynthesis (AP), stomatal conductance (Cs), soluble protein (SP), ribulose bisphosphate carboxylase (RuBisCO), and chlorophyll (Chl) were monitored in four main-stem cotton leaves which emerged at approximately 2 week intervals. The leaf which emerged during vegetative growth (48 days after planting) had higher AP, SP, and RuBisCO levels than that present in any leaves which emerged during fruit development. The last leaf studied (89 days after planting) was still present after boll maturation was completed and exhibited a rejuvenation in AP, SP, RuBisCO, and Chl starting at 30 days after leaf emergence. At 96 days after planting, the P700 Chl a-protein complex (PSI) was virtually absent from the leaves that emerged at 48 and 62 days after planting. The light harvesting Chl a/b complex was still present in these leaves, indicating greater degradation of PSI. The data emphasize the influence of developing fruit on concurrently developing leaves, an effect which was alleviated after boll maturation was completed. The declining AP per unit leaf area and smaller leaf size at the top of the plant results in a reduced photosynthetic potential of successively later emerging leaves. This reduction in leaf AP is consistent with earlier reported seasonal canopy photosynthesis patterns. 相似文献
15.
Leaf water potential, leaf relative water content, and relative transpiration of barley were determined daily under greenhouse conditions at 3 growth stages: tillering to boot, boot to heading, and heading to maturity. The leaf moisture characteristic curve (relative water content versus leaf water potential) was the same for leaves of the same age growing in the same environment for the first 2 stages of growth, but shifted at the heading to maturity stage to higher leaf relative water content for a given leaf water potential. Growth chamber experiments showed that the leaf moisture characteristic curve was not the same for plants growing in different environments.
Relative transpiration data indicated that barley stomates closed at a water potential of about −22 bars at the 3 stages studied.
The water potential was measured for all the leaves on barley to determine the variation of water potential with leaf position. Leaf water potential increased basipetally with plant leaf position. In soil with a moisture content near field capacity a difference of about 16.5 bars was observed between the top and bottom leaves on the same plant, while in soil with a moisture content near the permanent wilting point the difference was only 5.6 bars between the same leaf positions.
相似文献16.
Leaf area and photosynthesis of newly emerged trifoliolate leaves are regulated by mature leaves in soybean 总被引:1,自引:0,他引:1
Yushan Wu Wanzhuo Gong Yangmei Wang Taiwen Yong Feng Yang Weigui Liu Xiaoling Wu Junbo Du Kai Shu Jiang Liu Chunyan Liu Wenyu Yang 《Journal of plant research》2018,131(4):671-680
Leaf anatomy and the stomatal development of developing leaves of plants have been shown to be regulated by the same light environment as that of mature leaves, but no report has yet been written on whether such a long-distance signal from mature leaves regulates the total leaf area of newly emerged leaves. To explore this question, we created an investigation in which we collected data on the leaf area, leaf mass per area (LMA), leaf anatomy, cell size, cell number, gas exchange and soluble sugar content of leaves from three soybean varieties grown under full sunlight (NS), shaded mature leaves (MS) or whole plants grown in shade (WS). Our results show that MS or WS cause a marked decline both in leaf area and LMA in newly developing leaves. Leaf anatomy also showed characteristics of shade leaves with decreased leaf thickness, palisade tissue thickness, sponge tissue thickness, cell size and cell numbers. In addition, in the MS and WS treatments, newly developed leaves exhibited lower net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (E), but higher carbon dioxide (CO 2 ) concentration in the intercellular space (Ci) than plants grown in full sunlight. Moreover, soluble sugar content was significantly decreased in newly developed leaves in MS and WS treatments. These results clearly indicate that (1) leaf area, leaf anatomical structure, and photosynthetic function of newly developing leaves are regulated by a systemic irradiance signal from mature leaves; (2) decreased cell size and cell number are the major cause of smaller and thinner leaves in shade; and (3) sugars could possibly act as candidate signal substances to regulate leaf area systemically. 相似文献
17.
S. Jiménez F. Morales A. Abadía J. Abadía M. A. Moreno Y. Gogorcena 《Plant and Soil》2009,315(1-2):93-106
Iron is an essential micronutrient for plant growth and development, involved in key cellular processes. However, the distribution of Fe in plant tissues is still not well known. In the so-called Fe chlorosis paradox, leaves of fruit trees grown in the field usually have high concentrations of Fe but still are Fe-deficient. Leaves of the Prunus rootstock GF 677 (P. dulcis?×?P. persica) grown in hydroponics have been used to carry out two-dimensional (2-D) nutrient mapping by synchrotron radiation-induced X-ray fluorescence. Iron-deficient leaves accumulated more Fe in the midrib and veins, with Fe concentration being markedly lower in mesophyll leaf areas. The effects of Fe deficiency and Fe re-supply on leaf chlorophyll concentration and on the distribution of Fe and other nutrients within different plant tissues have been investigated in the same plants. After Fe re-supply, leaf Fe concentrations increased largely in all leaf types. However, whereas re-greening was almost completely achieved in apical leaves, in some expanded leaves the increase in chlorophyll concentration was only moderate. Therefore, after Fe re-supply Fe-deficient expanded leaves of the Prunus rootstock GF 677 had significant increases in Fe concentration but were still chlorotic. This is similar to what occurs in leaves of peach trees in field conditions, opening the possibility that this system could be used as a model to study the Fe chlorosis paradox. 相似文献
18.
Herbaceous species possess several mechanisms to compensate for tissue loss. For clonal herbaceous species, clonal integration may be an additional mechanism. This may especially hold true when tissue loss is very high, because other compensatory mechanisms may be insufficient. On inland dunes in northern China, we subjected Bromus ircutensis and Psammochloa villosa ramets within 0.5 m×0.5 m plots to three clipping treatments, i.e., no clipping, moderate (50% shoot removal) and heavy clipping (90% shoot removal), and kept rhizomes at the plot edges connected or disconnected. Moderate clipping did not reduce ramet, leaf or biomass density of either species. Under moderate clipping, rhizome connection significantly improved the performance of Psammochloa, but not that of Bromus. Heavy clipping reduced ramet, leaf and biomass density in the disconnected plots of both species, but such negative effects were negated or greatly ameliorated when the rhizomes were connected. Therefore, clonal integration contributed greatly to the compensatory growth of both species. The results suggest that clonal integration is an additional compensatory mechanism for clonal plants and may be important for their long-term persistence in the heavily grazed regions in northern China. 相似文献
19.
Abstract. Chlorophyll fluorescence emission spectra and the kinetics of 685 mm fluorescence emission from wheat leaf tissue and thylakoids isolated from such tissue were examined as a function of excitation wavelength. A considerable enhancement of fluorescence emission above 700 nm relative to that at 685 nm was observed from leaf tissue when it was excited with 550 nm rather than 450 nm radiation. Such excitation wavelength dependent changes in the emission spectrum occurred over an excitation spectral range of 440–660 nm and appeared to be directly related to the total quantity of radiation absorbed at a given excitation wavelength. Experiments with isolated thylakoid preparations demonstrated that changes in the fluorescence emission spectrum of the leaf were attributable to the optical properties of the leaf and were not due to the intrinsic characteristies of the thylakoid photochemical apparatus. This was not the case for the observed excitation wavelength dependent changes in the 685 nm fluorescence induction curve obtained from leaf tissue infiltrated with DCMU. Excitation wavelength dependent changes in the ratio of the variable to maximal fluorescence emission and the shape of the variable fluorescence induction were observed for leaf tissue. Isolated thylakoid studies showed that such changes in the leaf fluorescence kinetics were representative of the way in which the photochemical apparatus in vivo was processing the absorbed radiation at the different excitation wavelengths. The results are considered in the context of the use of fluorescence emission characteristics of leaves as non-destructive probes of the photochemical apparatus in vivo. 相似文献
20.
Polar auxin transport (PAT) is a major determinant of plant morphology and internal anatomy with important roles in vascular patterning, tropic growth responses, apical dominance and phyllotactic arrangement. Woody plants present a highly complex system of vascular development in which isolated bundles of xylem and phloem gradually unite to form concentric rings of conductive tissue. We generated several transgenic lines of hybrid poplar (Populus tremula x alba) with the auxin-responsive DR5 promoter driving GUS expression in order to visualize an auxin response during the establishment of secondary growth. Distinct GUS expression in the cambial zone and developing xylem-side derivatives supports the current view of this tissue as a major stream of basipetal PAT. However, we also found novel sites of GUS expression in the primary xylem parenchyma lining the outer perimeter of the pith. Strands of primary xylem parenchyma depart the stem as a leaf trace, and showed GUS expression as long as the leaves to which they were connected remained attached (i.e., until just prior to leaf abscission). Tissue composed of primary xylem parenchyma strands contained measurable levels of free indole-3-acetic acid (IAA) and showed basipetal transport of radiolabeled auxin (3H-IAA) that was both significantly faster than diffusion and highly sensitive to the PAT inhibitor NPA. Radiolabeled auxin was also able to move between the primary xylem parenchyma in the interior of the stem and the basipetal stream in the cambial zone, an exchange that was likely mediated by ray parenchyma cells. Our results suggest that (a) channeling of leaf-derived IAA first delineates isolated strands of pre-procambial tissue but then later shifts to include basipetal transport through the rapidly expanding xylem elements, and (b) the transition from primary to secondary vascular development is gradual, with an auxin response preceding the appearance of a unified and radially-organized vascular cambium. 相似文献