共查询到20条相似文献,搜索用时 15 毫秒
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An Endocrine-Exocrine Switch in the Activity of the Pancreatic Homeodomain Protein PDX1 through Formation of a Trimeric Complex with PBX1b and MRG1 (MEIS2) 总被引:11,自引:7,他引:4 下载免费PDF全文
Galvin H. Swift Ying Liu Scott D. Rose Larry J. Bischof Scott Steelman Arthur M. Buchberg Christopher V. E. Wright Raymond J. MacDonald 《Molecular and cellular biology》1998,18(9):5109-5120
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Shen WF Rozenfeld S Kwong A Köm ves LG Lawrence HJ Largman C 《Molecular and cellular biology》1999,19(4):3051-3061
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In V(D)J recombination, the RAG1 and RAG2 proteins are the essential components of the complex that catalyzes DNA cleavage. RAG1 has been shown to play a central role in DNA binding and catalysis. In contrast, the molecular roles of RAG2 in V(D)J recombination are unknown. To address this, we individually mutated 36 evolutionarily conserved basic and hydroxy group containing residues within RAG2. Biochemical analysis of the recombinant RAG2 proteins led to the identification of a number of basic residue mutants defective in catalysis in vitro and V(D)J recombination in vivo. Five of these were deficient in binding of the RAG1-RAG2 complex to its cognate DNA target sequence while interacting normally with RAG1. Our findings provide support for the direct involvement of RAG2 in DNA binding during all steps of the cleavage reaction. 相似文献
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mTFE3, an X-linked transcriptional activator containing basic helix-loop-helix and zipper domains, utilizes the zipper to stabilize both DNA binding and multimerization. 总被引:2,自引:0,他引:2 下载免费PDF全文
C Roman A G Matera C Cooper S Artandi S Blain D C Ward K Calame 《Molecular and cellular biology》1992,12(2):817-827
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