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Paul Coppo Michael Schwarzinger Marc Buffet Alain Wynckel Karine Clabault Claire Presne Pascale Poullin Sandrine Malot Philippe Vanhille Elie Azoulay Lionel Galicier Virginie Lemiale Jean-Paul Mira Christophe Ridel Eric Rondeau Jacques Pourrat Stéphane Girault Dominique Bordessoule Samir Saheb Michel Ramakers Mohamed Hamidou Jean-Paul Vernant Bertrand Guidet Martine Wolf Agnès Veyradier for the French Reference Center for Thrombotic Microangiopathies? 《PloS one》2010,5(4)
Severe ADAMTS13 deficiency occurs in 13% to 75% of thrombotic microangiopathies (TMA). In this context, the early identification of a severe, antibody-mediated, ADAMTS13 deficiency may allow to start targeted therapies such as B-lymphocytes-depleting monoclonal antibodies. To date, assays exploring ADAMTS13 activity require skill and are limited to only some specialized reference laboratories, given the very low incidence of the disease. To identify clinical features which may allow to predict rapidly an acquired ADAMTS13 deficiency, we performed a cross-sectional analysis of our national registry from 2000 to 2007. The clinical presentation of 160 patients with TMA and acquired ADAMTS13 deficiency was compared with that of 54 patients with detectable ADAMTS13 activity. ADAMTS13 deficiency was associated with more relapses during treatment and with a good renal prognosis. Patients with acquired ADAMTS13 deficiency had platelet count <30×109/L (adjusted odds ratio [OR] 9.1, 95% confidence interval [CI] 3.4–24.2, P<.001), serum creatinine level ≤200 µmol/L (OR 23.4, 95% CI 8.8–62.5, P<.001), and detectable antinuclear antibodies (OR 2.8, 95% CI 1.0–8.0, P<.05). When at least 1 criteria was met, patients with a severe acquired ADAMTS13 deficiency were identified with positive predictive value of 85%, negative predictive value of 93.3%, sensitivity of 98.8%, and specificity of 48.1%. Our criteria should be useful to identify rapidly newly diagnosed patients with an acquired ADAMTS13 deficiency to better tailor treatment for different pathophysiological groups. 相似文献
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E.J.J. van Zoelen E.C.M. van der Neut-Kok J. de Gier L.L.M. van Deenen 《生物化学与生物物理学报:生物膜》1975,382(3):463-469
The osmotic behaviour of Acholeplasma laidlawii B cells was investigated with combined spectrophotometric and enzymatic measurements. The conclusion could be drawn that this osmotic behaviour depends largely on the physical state of the membrane lipids. When part of the membrane lipids is in the liquid-crystalline phase the cell is able to swell and behaves as a good osmometer. However, when the membrane lipid is in the gel phase, the cell is unable to swell and the change in absorbance of the cell suspension is then completely due to lysis. 相似文献
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Jones G Barker A 《The Clinical biochemist. Reviews / Australian Association of Clinical Biochemists》2008,29(Z1):S93-S97
Recommended elements of a process for establishing a reference interval: Define the analyte (measurand) for which the reference interval is being established, the clinical utility, biological variation and major variations in form. Define the method used, the accuracy base, and analytical specificity. Define important pre-analytical considerations together with any actions in response to the interference. Define the principle behind the reference interval (i.e. central 95% etc.). Describe the data source(s), including: number of subjects, nature of subjects, exclusions, pre-analytical factors, statistical measures, outliers excluded and analytical method. Define considerations of partitioning based on age, sex etc. Define the number of significant figures, i.e. the degree of rounding. Define the clinical relevance of the reference limits. Consider the use of common reference intervals. Decision and implementation. 相似文献
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