Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)

From a genomic library enriched for GATA/CTAT and GAAA/CTTT repeats, 12 polymorphic microsatellite markers were developed for the Lanyu scops owl (Otus elegans botelensis). Polymorphism of these loci was evaluated in a sample of 58 adult individuals of unknown relationship. The allele numbers of each locus were from five to 25 and the observed heterozygosity of each locus ranges from 0.707 to 0.914. When using these loci in parentage assignment, the probability of false parent exclusion is greater than 0.999. All loci conformed to Hardy–Weinberg expectations.     

The demography of an increasing insular Eurasian Scops Owl (Otus scops) population in western France

Rapid population declines of many long-distance Afro-Palaearctic migratory bird species are ongoing across Europe but the demographic drivers are often poorly understood, thereby limiting the development of appropriate conservation actions. Using long-term population monitoring (39 years), capture–mark–recapture data and a matrix model, we estimated demographic parameters and the effect of climate variables on adult survival, and modelled the dynamics of an increasing population of Eurasian Scops Owls Otus scops in a landscape with agricultural abandonment in western France. The observed mean annual population growth rate was 1.055 (from 68 to 523 territorial males between 1981 and 2019). Over the study period, clutch size and hatching success were stable, but fledging success and breeding success showed slight negative trends, probably due to density-dependence. Survival varied with age, with an increase during early life and evidence for rapid senescence from 4 years old. Adult survival remained stable and was positively linked to the amount of autumn rainfall in the Sahel and to the winter North Atlantic Oscillation. Survival of younger age-classes made the largest contribution to the variance of the population growth rate, followed by clutch size, fledging success and survival of older birds. Such a long-term population increase in a landscape where intensive agriculture has decreased by 64.6% sheds some new light on the causes of the decline of European Scops Owl and other Afro-Palaearctic bird populations. We infer some of the possible causes of this large-scale decline, in particular food shortage, and discuss conservation measures that could be applicable to reverse this trend.     

Nutritional implications of staple food crop successions in Usambara,Tanzania

Archival and ethnographic research carried out in northeastern Tanzania provides evidence of significant changes in production and consumption of staple food crops between 1850 and the present. The banana, which provided the bulk of calories and other nutrients in precolonial times, has been successively replaced by Irish potatoes, maize, and cassava in different localities. The reasons for the adoption of each of these introduced crops are explored, and their implications for human nutrition in the area are discussed.     

Sex-biased initial eggs favours sons in the slightly size-dimorphic Scops owl (Otus scops)

There is considerable controversy concerning the origin of Iberian populations of the Mediterranean chameleon, Chamaeleo chamaeleon . Current opinion dictates that Spanish populations result from introductions during the 18^th and 19^th centuries, with subsequent translocations from the original populations to other parts of Spain. The Portugese population in the Algarve is believed to have been introduced from Africa or Spain during the 1920s. However, Holocene remains of chameleons suggest that the Malaga population at least could have a much older origin. Analysis of sequences from the mitochondrial 16S ribosomal RNA gene of samples from the Iberian Peninsula and North Africa revealed a double origin for the Iberian population. The Mediterranean Iberian (Malaga) population is closely related to Mediterranean North African populations, with Atlantic Iberian populations more closely related to populations of the Atlantic coast of North Africa. The overall genetic differentiation and diversity observed was very low, preventing precise dating of the colonization events. However this low level of differentiation is not consistent with Plio-Pleistocene colonization, the assumed timing for a natural colonization event and suggests that chameleons were probably introduced twice by man in the recent past. © 2002 The Linnean Society of London, Biological Journal of the Linnean Society , 2002, 75 , 1–7.     

Breeding seasonality and population dynamics of three rodent species in the Magamba Forest Reserve, Western Usambara Mountains, north-east Tanzania

We investigated breeding seasonality and population dynamics of three rodent species, Lophuromys flavopuncatus, Grammomys dolichurus and Praomys delectorum, in the Magamba Forest, Western Usambara Mountains, north‐east Tanzania. Capture–mark–recapture studies were conducted in 2002–2004. Reproductive conditions of males and females showed temporal variations, an indication of breeding seasonality. Animals were reproductively active between February and May. Rainfall in November–January was instrumental for the onset of breeding and continued throughout the wet season. The recruitment of new individuals born during the season led to highest population densities between end of May and August. Populations declined progressively towards the end of the dry season (September–October). Only P. delectorum showed a marked density increase during January–February, indicating greater survival and/or recruitment during the November–January rains. The study shows that despite a relatively stable environment of the forest reserve, rainfall has strong influence on reproduction and population dynamics, probably because of its effect on primary food resources.     

Pinpointing baobab (Adansonia digitata [Linn. 1759]) population hotspots in the semi-arid areas of Tanzania

The impact of unsustainable land-use conversions, changes in climate and anthropogenic activities on abundance and distribution of baobab populations was assessed in semi-arid regions of Tanzania. Baobabs were sampled in plots of 1 km long and a 50 m wide, which were carried out in 337 grids located in different land-use types. Transects for each land-use type were located using a stratified random sampling technique to compare baobab population variations and occurrences in semi-arid areas of Tanzania. Baobab density was found to be highest in strictly protected areas and the lowest density in unprotected areas, suggesting that anthropogenic activities coupled with local management practices within land-uses may be influencing its viability in semi-arid areas. In species like this, with less and slow recruitment rate, it takes a long time to bring the population to recovery when substantial disturbance and overutilisation have reduced the populations to certain levels. Thus, increased human and climate change pressures on land are likely to drive the species to extinction in these fragmented populations.     

Missing piece of top predator-based conservation: Demographic analysis of an owl population on a remote subtropical island

Top predators are frequently the target of conservation programs. Owls are such predators. However, previous studies of owls are biased to species occurring in temperate regions, whereas most owl species occur in tropical or subtropical regions and are understudied. Furthermore, owls are often endemic to islands and of unknown conservation status. Demographic data for such species are especially scarce although they are essential for initiating and promoting their conservation. As a case study of demographic analysis of owls in a tropical or subtropical area and on islands, we applied an integrated population model to 7-year monitoring data (2012–2018) of the Ryukyu Scops Owl population on Minami-daito Island, Japan. We used survival history data from 903 individuals, reproduction and sex ratio data from 213 broods, and count data of 2,526 individuals in total. Long-term averages of annual survival rates were 0.73 for adult females and 0.74 for adult males, although the sexual difference was not significant. Sex ratio estimates fluctuated annually and long-term averages were slightly skewed to males: 0.51 among fledglings, 0.54 among yearlings, and 0.52 among adults. Long-term averages of population size were estimated to be 273.4 females and 296.8 males. The long-term average of population growth rate was 0.98, suggesting a slightly declining trend. It was fortunate to recognize the declining trend during the early phase. Considering the general lack of fundamental ecological data on owls of tropical or subtropical areas and on islands, it seems likely that many endangered owl populations await conservation efforts.     

Distribution, density, diet and productivity of the Scops Owl Otus scops in the Italian Alps

The Scops Owl Otus scops is probably the least known European owl. We surveyed Scops Owls in the Trento region (6200 km²) of the central–eastern Italian Alps between 1995 and 2003 and we intensively monitored a subpopulation in a 50‐km² plot between 2000 and 2003. In the whole region, we found 81 territories concentrated in 21, low‐elevation 100‐km² quadrats. Most territories were associated with villages surrounded by extensively managed grassland (79%), arid areas with rocky outcrops and xerophytic vegetation (12%) and/or large urban areas and parks (6%). In the 50‐km² plot, density varied between 52 and 64 territories/100 km² annually. Territories were either solitary or clumped in loose colonies of 2–7 pairs. In contrast to previous studies, most nests used for laying were in holes and cracks of buildings (95%, n = 20). This may have been favoured by thermal and foraging advantages, but also involved some costs, such as predation by domestic cats and collision with cars. Median laying date was 29 May (n = 16) and the mean number of fledged young was 1.37 (n = 30), 1.95 (n = 21) and 2.00 (n = 20) per territorial, breeding and successful pair, respectively. The diet was dominated by grasshoppers of the family Tettigoniidae. Compared to previous studies, this population showed medium to high density and low productivity. The species seems to be dependent on traditional, extensive agro‐pastoralism and the main conservation threats include habitat loss through land abandonment and consequent forest expansion, which are probably best halted through subsidy schemes. From our results and published data, we estimate the population of the Scops Owl in the Italian Alps at 230–500 territories. There is an urgent need for further data on this largely overlooked species, especially from its Mediterranean strongholds.     

High frequency of extrapair copulation with low level of extrapair fertilization in the Lanyu scops owl Otus elegans botelensis

The genetic mating system is known for only a few species of owls. Most of them are genetically monogamous. The Lanyu scops owl Otus elegans botelensis breeds in high density in the forests of Lanyu (Orchid Island), southeast of Taiwan. Because extra‐pair copulations (EPCs) have frequently been observed, we suspected a high degree of extra‐pair fertilizations (EPFs). Through intensive field observations we quantified both within‐pair and extra‐pair copulations of this owl, and used a set of microsatellite loci to assign the parentage of 200 offspring from 108 families collected between 1999 and 2004. Among copulations for which we could identify both participants, 19.77% were EPC. However, no EPC was observed in the period just before egg laying. We found only two cases of parentage mismatch among 108 broods. The first case was most likely a case of brood usurpation, which would be the first case reported in the Strigidae. The second case was an EPF resulting in one offspring. Our study found that parentage mismatch in the Lanyu scops owls to be extremely low, therefore reproductive success can be estimated accurately by simply counting chicks in nests.     

High frequency of extrapair copulation with low level of extrapair fertilization in the Lanyu scops owl Otus elegans botelensis

The genetic mating system is known for only a few species of owls. Most of them are genetically monogamous. The Lanyu scops owl Otus elegans botelensis breeds in high density in the forests of Lanyu (Orchid Island), southeast of Taiwan. Because extra-pair copulations (EPCs) have frequently been observed, we suspected a high degree of extra-pair fertilizations (EPFs). Through intensive field observations we quantified both within-pair and extra-pair copulations of this owl, and used a set of microsatellite loci to assign the parentage of 200 offspring from 108 families collected between 1999 and 2004. Among copulations for which we could identify both participants, 19.77% were EPC. However, no EPC was observed in the period just before egg laying. We found only two cases of parentage mismatch among 108 broods. The first case was most likely a case of brood usurpation, which would be the first case reported in the Strigidae. The second case was an EPF resulting in one offspring. Our study found that parentage mismatch in the Lanyu scops owls to be extremely low, therefore reproductive success can be estimated accurately by simply counting chicks in nests.     

Inbreeding and inbreeding depression in a threatened endemic plant,the African violet (Saintpaulia ionantha ssp. grotei), of the East Usambara Mountains,Tanzania

Mating among closely‐related individuals in small and isolated plant populations may result in reduced vigour of the inbred offspring, i.e. inbreeding depression, especially in naturally outbreeding plants. Occurrence of inbreeding and inbreeding depression was studied in Saintpaulia ionantha ssp. grotei, a threatened endemic plant species with a narrow ecological amplitude from the East Usambara Mountains. The level of inbreeding (measured as the fixation index, F) was investigated in twelve populations by analyzing variation at one microsatellite marker locus. The effect of one generation of selfing and outcrossing on the progeny fitness was studied by controlled crosses in two small patches that differ in the level isolation. The fixation index (F) across the populations was on the average 0.21 and varied among the populations from substantial inbreeding (F = 0.58) to surplus heterozygosity (F = −0.29). High inbreeding depression (δ) was observed at early and late stages of the life‐cycle. The isolated patch exhibited lower inbreeding depression than did the non‐isolated patch. The results of this study suggest that inbreeding and subsequent inbreeding depression are potential threats to the survival of Saintpaulia populations.     

Impact of habitat disturbance in the wetland forests of East Usambara,Tanzania

We evaluated habitat characteristics of East Usambara wetland forests. The abundance and species composition in the tree, shrub and herbaceous layers were enumerated in two sets of nested plots, one set in a natural wetland forest and the second in a wetland forest that had been disturbed by small‐scale gold mining activities. Each set had thirty‐six 1 m × 1 m plots for herbs, inside nine 5 m × 5 m plots for shrubs, in three 20 m × 20 m plots for trees. The habitat profile of herbaceous – shrub – tree layers was found to be sharply different from one obtained in previous studies at the surrounding nonwetland forests as were species composition and abundance. Unlike in the nonwetland forests, the herbaceous layer was thick, the shrub layer very thin and the woody species density and richness much lower. Disturbance significantly reduced woody cover and changed species composition in the herbaceous layer. Recovery of the woody vegetation was low. Wetland forests in the East Usambaras form a small fraction of the total area, but they are a unique biodiversity repository, they appear to be an important carbon dioxide sink and to reserve and purify water. They are sensitive to disturbance and need protection.     

Power to detect trends in ecological indicators in the East Usambara Mountains, Tanzania

We evaluated the statistical power of monitoring protocols to detect, over a 10‐year period, trends in indices of abundance of primates, hornbills and forest interior dung beetles and growth rates of epiphytic ferns in the Amani Nature Reserve, in the East Usambara Mountains, Tanzania. Local technicians are responsible for the day‐to‐day gathering of data. The existing monitoring protocols for blue monkey, silver‐cheeked hornbills, trumpeter hornbills, forest interior dung beetles and Asplenium nidus have sufficient statistical power (>0.80) to detect trends of 65% or less over a 10‐year period. Monitoring protocols for black and white colobus and Asplenium holstii have lower statistical power (<0.80). We therefore conclude that the majority of monitoring protocols of the East Usambara Ecological Monitoring Project have the capacity both logistically and statistically to detect long‐term trends in important functional groups in the East Usambara Mountains.     

Seasonal patterns of rodents, fleas and plague status in the Western Usambara Mountains, Tanzania

Field and commensal rodents were live-trapped at three villages in an active focus of plague (Yersinia pseudotuberculosis pestis) in Lushoto District, Western Usambara Mountains, Tanga Region, Tanzania, from December 1983 to November 1984. Their flea ectoparasites were collected, identified and counted. The rodent carcasses were serologically examined for specific plague antibodies and antigens, and bacteriologically examined for bipolar staining bacilli. A total of 1758 traps were set during the 12-month period and 924 animals were caught. From these, 1037 fleas were collected. Rattus rattus (L.), Praomys natalensis (Smith) and Lophuromys flavopunctatus Thomas comprised the largest proportions of the rodent population, while Dinopsyllus lypusus Jordan & Rothschild, Ctenophthalmus calceatus Waterston and Xenopsylla brasiliensis (Baker) were the dominant flea species. Rodents were most abundantly trapped during December and January. Flea indices were highest from December to May. Human plague was most active from November to March. Rodents contained plague antibodies every month except May and July, with a peak in September. Plague antigens and bipolar bacilli were detected in rodent organs during January-April. From the product of abundance and infection rate, the most prevalent rodent hosts of plague appeared to be R. rattus, Otomys angoniensis Wroughton, P. natalensis and Pelomys fallax (Peters). Continuous integrated control of rodents and fleas was recommended, reinforced by quarantine and maintenance of a surveillance service for clinical detection, diagnosis and treatment of patients in the plague endemic area.     

Species diversity and population dynamics of rodents in a farm‐fallow field mosaic system in Central Tanzania

A Capture‐Mark‐Recapture study was undertaken in Central Tanzania to compare variations in community structure and population dynamics of rodents in two types of habitats. The study was conducted in fallow field mosaic habitat dominated by perennial and annual grasses (grid BEA) and a more heterogeneous habitat (grid BEB) which was previously woodland cleared of most trees with vegetation dominated by shrubs, bushes, scattered trees and perennial grass. The relative abundance of rodents in BEA was: Mastomys natalensis (73.5%) > Aethomys chrysophilus (8.9%) > Gerbilliscus vicina (7.3%) > Arvicanthis neumanni (6.1%) > Acomys spinosissimus (4.1%) and for grid BEB: M. natalensis (67.6%) > G. vicina (11.2%) > A. neumanni (10.3%) > A. chrysophilus (7.6%) > A. spinosissimus (2.9%). Graphiurus sp., Mus minutoides, Saccostomus mearnsi, Lemniscomys striatus and L. griselda were rare and only occasionally trapped in BEB. Spatial variations in population density were non‐significant except for A. chrysophilus. Significant temporal variations within grids were observed, with synchrony of population peaks for some species. The rare species boosted species richness of grid BEB rather artificially, without significantly contributing to higher species diversity. Temporal variations in Simpson’s Diversity indices between grids were non‐significant except for three out of twenty‐one trapping sessions.     

Diet of the Silvery-cheeked Hornbill Bycanistes brevis during the breeding season in the East Usambara Mountains,Tanzania

The breeding season diet and nesting characteristics of the Silvery-cheeked Hornbill Bycanistes brevis are poorly known. To further understand these aspects of the breeding biology of this hornbill species, 14 nests were studied in and around Amani Nature Reserve located in the East Usambara Mountains, Tanzania. Nesting tree species were identified and the diet composition of nesting hornbills was evaluated between July and November 2001. The ejecta from each nest were collected, inventoried, identified (as completely as possible) and enumerated. Food items were categorised as plant, vertebrate or invertebrate. Plants, represented largely by fruits, were the dominant food type (n = 861), followed by invertebrates (n = 306; mainly millipedes and beetles), and vertebrates (n = 15; mainly smaller birds and chameleons). A comparison of results from the current study to other nesting observations made approximately seven decades earlier in the same area suggest that (1) the invasive tree species Maesopsis eminii, which was the most common food type consumed (n = 4 539 seeds), has become a favoured new food source in the breeding season, and (2) the breeding season appears to have shifted to an earlier period, potentially due to the fruiting phenology and abundance of Maesopsis eminii.     

Predicting fluctuations of reintroduced ibex populations: the importance of density dependence, environmental stochasticity and uncertain population estimates

1. Development of population projections requires estimates of observation error, parameters characterizing expected dynamics such as the specific population growth rate and the form of density regulation, the influence of stochastic factors on population dynamics, and quantification of the uncertainty in the parameter estimates. 2. Here we construct a Population Prediction Interval (PPI) based on Bayesian state space modelling of future population growth of 28 reintroduced ibex populations in Switzerland that have been censused for up to 68 years. Our aim is to examine whether the interpopulation variation in the precision of the population projections is related to differences in the parameters characterizing the expected dynamics, in the effects of environmental stochasticity, in the magnitude of uncertainty in the population parameters, or in the observation error. 3. The error in the population censuses was small. The median coefficient of variation in the estimates across populations was 5.1%. 4. Significant density regulation was present in 53.6% of the populations, but was in general weak. 5. The width of the PPI calculated for a period of 5 years showed large variation among populations, and was explained by differences in the impact of environmental stochasticity on population dynamics. 6. In spite of the high accuracy in population estimates, the uncertainty in the parameter estimates was still large. This uncertainty affected the precision in the population predictions, but it decreased with increasing length of study period, mainly due to higher precision in the estimates of the environmental variance in the longer time-series. 7. These analyses reveal that predictions of future population fluctuations of weakly density-regulated populations such as the ibex often become uncertain. Credible population predictions require that this uncertainty is properly quantified.