Redescription of four species of Mehdiella from Testudinidae, with a key to the species and discussion on the relationships among the species of this genus

Four species of the genus Mehdiella Seurat, 1918 are redescribed: M. cristata Petter, 1966 and M. stylosa dollfusi Petter, 1966, parasite of Pyxix arachnoides Bell, 1827 from Madagascar, M. s. stylosa (Thapar, 1925) and M. uncinata (Drasche, 1884), parasite of Testudo graeca Linneaus, 1758, Testudo hermanni Gmelin, 1789 and Testudo horsfieldii (Gray, 1844) from Palaearctic region. Light microscopy and scanning electron microscopy (SEM) studies revealed new informations on the morphology of these species. On the basis of this morphological study, the sub-species Mehdiella stylosa dollfusi and M. s. stylosa are raised to level of species. The position of Mehdiella cristata among the species of the genus Mehdiella and the relationships among the species of the genus Mehdiella are discussed. A key to the eight valid species Mehdiella is given.     

Revision of the enigmatic Australasian genus Miropotes Nixon (Hymenoptera: Braconidae: Microgastrinae), with comments on the phylogenetic importance of the female ovipositor system

Abstract. The endemic Australasian microgastrine genus Miropotes Nixon, previously known from two species, M. creon Nixon from Tasmania and M. petiolaris (Szépligeti) from N.S.W., is revised. A further eight species are described as new (M. burringbaris, M. boothis, M. chookolis, M. goobitis, M. kilkulunis, M. cadgeis, M. katois and M. thuraris) , two of which represent the first record of the genus outside continental Australia (viz New Guinea, New Hebrides and New Caledonia). A key to species is presented along with information on the first host record for the genus. Relationships between Miropotes and other microgastrine genera are discussed, particularly in reference to the structure of the ovipositor and hypopygium, which have bearing on the current tribal classification of the subfamily.     

A revision of the genus Mouretia (Eubiaceae)

Four species are recognised in Mouretia, a genus previously thought to be monospecific. Two new species M. vietnamensis and M. larsenii are described, and M. tonkinensis var_: inequalis is elevated to the rank of species. All species are illustrated. The chromosome number far M. larsenii is given. The character states for the genus are presented. A comparison between Mouretia and Mycetia show them to share most of their character states. It is suggested that the two genera form a monophyletic group, that should be included in the tribe Hedyotideae.     

中国水柏枝属植物的地理分布、濒危状况及其保育策略

水柏枝属(MyricariaDesv.)植物主要分布于中国青藏高原及其邻近地区,为欧亚温带高山植物类群,多为河岸带植物,是分布区湿地生态系统和荒漠生态系统的重要组分。为评价这些特殊生态系统关键植物的分布及受威胁现状,对中国水柏枝属植物的地理分布和濒危状况进行了调查。调查发现,该属中国分布的11个分类群均受到生存胁迫,其中疏花水柏枝即将成为野外灭绝种,宽叶水柏枝已属极危种,心叶水柏枝为濒危种,秀丽水柏枝、泽当水柏枝、小花水柏枝、卧生水柏枝和匍匐水柏枝为渐危种,具鳞水柏枝、三春水柏枝和宽苞水柏枝为受胁种。结果表明,以水利建设为主的人为干扰和土地沙化、气温升高带来的环境改变是导致该属植物生存胁迫的主要原因。最后探讨了水柏枝属这类河岸带湿地和荒漠生境植物的保育策略。     

Review of the genus Myrabolia Reitter (Coleoptera: Cucujoidea: Myraboliidae)

Abstract  Species of the Australian genus Myrabolia are reviewed. Lectotypes are designated for Silvanus brevicornis Erichson, Myrabolia haroldiana Reitter, M. lindensis Blackburn and M. longicornis Blackburn. The following new species are described and illustrated: M. australis, M. blackburni , M. elongata , M. kioloa , M. lawrencei , M. micra , M. pelion and M. tasmanica. Ocholissa leai Grouvelle, originally described in Colydiidae, is here transferred to Myrabolia as M. leai (Grouvelle) comb. n. The nomenclatural history, a diagnosis and distribution data are provided for each species. A key to the species of the genus is presented.     

中国纩蚜属研究及新种新亚种记述(同翅目:纩蚜科)(英文)

通过对中国纩蚜属的研究,作者提出纩蚜属的现代地理分布呈岛状分布的格局;并推测该属的起源中心可能在欧洲,现代分化中心可能在中国的横断山脉地区。从动物地理的角度,探讨了该属内的系统发育关系。同时,发现一新种,即云杉纩蚜Mindaurspiceasuctusspnov.。正模为无翅孤雌蚜,副模为5只有翅孤雌蚜和8只无翅孤雌蚜,分布在云南省昆明市,辽宁省沈阳市和熊岳县。一新亚种,即冷杉纩蚜三圈亚种M.abietinustriprimesensorissp.nov.,正模为有翅孤雌蚜,副模为3只有翅孤雌蚜,分布在云南省丽江市玉龙山。一新纪录种,即冷杉纩蚜指名亚种M.abietinusabietinusKoch1856。并且给出种的检索表,模式标本存放在中国科学院动物研究所标本馆。     

Phylogenetic analysis of the genus Microbacterium based on 16S rRNA gene sequences

Abstract 16S rRNA gene (rDNA) studies of the six species of the genus Microbacterium, M. lacticum, M. laevaniformans, M. dextranolyticum, M. imperiale, M. arborescens and M. aurum , were performed and the primary structures were compared with those of 29 representative actinobacteria and related organisms. Phylogenetic analysis indicated that six species of the genus Microbacterium and representative four species of the genus Aureobacterium appear to be phylogenetically coherent as was suggested by Rainey et al., although the peptidoglycan types of these two genera are different (peptidoglycan type B1 or B2). Thus, the phylogenetical analyses revealed that members of actinobacteria with group B-peptidoglycan do not cluster according to their peptidoglycan types, but form compact cluster different from actinobacteria or actinomycetes with group A-peptidoglycan.     

A revised diagnosis of the feather mite genus Magimelia Gaud, 1961 (Pterolichoidea: Pterolichidae: Magimeliinae) and the description of three new species

Three new mite species of the genus Magimelia (Astigmata: Pterolichidae) are described from the plumage of various lapwings (Charadriidae: Vanellinae): M. breviloba n. sp. from Vanellus miles miles; M. thailandica n. sp. from V. indicus (type-host), V. duvaucelii and V. tricolor; and M. chilensis n. sp. from V. chilensis. An extended host range for M. dolichosikya Gaud, 1961 is given. A revised diagnosis of the genus and a key to known species are presented.     

猕猴属五个种mtDNA多态性研究

本文以10种限制性内切酶研究猕猴属5个种(Macaca mulatta.M.nemestrina.M.assemensis.M.thibetana,M arctoides)线粒体DNA进化。在13个个体中,共检出8种限制性类型。恒河猴种内存在广泛的线粒休DNA限制性片段长度多态性(RFLP)。结合日本猴(M.fuscata)的有关资料,构建了猕猴属6个种的分子系统树,并给出各个种的分化时间。结果表明,这6个种可分成4个类群,熊猴和藏酋猴、恒河猴和日本猴之间的遗传距离较近,可分别划为同一类群,红面猴与其他5种猴的遗传距离最远,在系统发生上分离最早。     

Phylogeny of the mud worm genus Marenzelleria (Polychaeta, Spionidae) inferred from mitochondrial DNA sequences

The taxonomy of the cryptic morphospecies of the mud worm genus Marenzelleria is particularly difficult and the phylogenetic relationship within the genus is unknown. Herein we reconstructed the phylogeny of all five species of this genus using sequence data of three mitochondrial genes (16SrDNA, cytochrome b, cytochrome oxidase subunit I) from 104 specimens out of 26 populations. For the three invasive species of the genus, Marenzelleria neglecta, M. viridis and M. arctia , individuals from native populations as well as from recently invaded populations were included. Nuclear 18S rDNA sequences were used to evaluate the appropriate outgroup taxon among several spionid polychaete species. The results supported the monophyly of Marenzelleria , and Malacoceros fuliginosus was found to be a suitable outgroup for the analysis of the mitochondrial gene segments. All phylogenetic reconstructions revealed a basal position of M. arctia and M. wireni , which have primarily Arctic distribution, with M. arctia obtaining the most basal position. Together with the present-day distribution of the species, this indicates an origin of the genus in the Arctic region. The relationship of the species M. neglecta , M. viridis and M. bastropi could not be resolved sufficiently due to genealogical discordance that might reflect relatively young cladogenetic events.     

New Harpacticoida (Crustacea, Copepoda) from the North Atlantic Ocean. V. Three New Species of Metahuntemannia Smirnov (Cletodidae)

Metahuntemannia triarticulata sp.n., M. arctica sp.n. and M. bifida sp.n. arc described. M. triurficulata and M. arctica belong to the 'spinosa' group within the genus Metahuntemannia Smirnov. From the closely related species, M. triarticulata differs in the possession of 3-segmented Enp P3, M. arctica in the segmentation of the Al, and both new species in the setation of their swimming legs P1–P5. M. hifida belongs to the 'talpa' group within Metahuntemannia and differs from the closely related species in the setation of the swimming legs P2–P4. The species relationships and the phylogeny within the genus are discussed. All species were collected at the Iceland-Faroe Ridge from depths between 435 and 2500 m.     

Javanese species of the mite genus Macrocheles (Arachnida: Acari: Gamasina: Macrochelidae)

Twelve mite species of the genus Macrocheles (Acari: Macrochelidae) were collected from the body surface of dung beetles in Java, Indonesia. Of these, three species, i.e., Macrocheles jabarensis, M. jonggolensis, and M. sukabumiensis, were described as new to science. Female of M. dispar was redescribed. Two species, i.e., M. baliensis and M. sukaramiensis, were recorded from Java for the first time. The occurrence of five species previously recorded from Java, i.e., M. hallidayi, M. kraepelini, M. limue, M. oigru, and M. merdarius, were reconfirmed. Taxonomic status of M. sp. aff. glaber was not settled in the present study, because we could not obtain the male and immatures which are indispensable for exact identification. In total 15 species of the genus Macrocheles, including 3 species already recorded but not collected in this research (M. crispa, M. krantzi, and M. subbadius), are known from Java up to date.     

A STUDY ON MINDARUS KOCH IN CHINA WITH DESCRIPTIONS OF NEW SPECIES AND NEW SUBSPECIES (HOMOPTERA: MIN-DARIDAE)*

Abstract This paper deals with the genus Mindam Koch from China. The phylogeny of the genus is discussed. M. keteleerifoliae is regarded as the most advanced species, M. obliquus and M. piceasuc-tus are more advanced than M. abietinus, M. japonicus and M. victoria. The origin centre of the genus may be in Europe and the differentiation centre of the genus may be in Hengduan Mountains Region of China. Geological distribution of the genus shows island-shaped pattern. Five species from China was described in this paper, including one new species, M. piceasuctus Zhang and Qiao, one new subspecies, M. abietinus triprimesensori Zhang and Qiao and one newly recorded species, M. abietinus abietinus Koch 1856. A key to the Chinese species of Mindarus is given. The specimens of five species from China are deposited in the Institute of Zoology, Academia Sinica.     

Real-time PCR assay for the detection of species of the genus Mannheimia

Infections caused by species of the genus Mannheimia cause diverse disease complexes in many wild and domestic animals worldwide. Fast and accurate detection of single species within the genus remains an unsolved problem till today. To resolve this diagnostic challenge, we developed a real-time PCR assay for the rapid and specific identification of five species of the genus Mannheimia (M. haemolytica, M. varigena, M. ruminalis, M. granulomatis and M. glucosida) from bacterial cultures and tissue samples. The assay was validated with reference strains, field isolates and bacteria spiked tissue samples. The sodA gene was used as target region for species-specific primer pairs. The real-time PCR assay demonstrated species specificity for all five examined Mannheimia spp. and a rapid test completion time of less than 5 h. This is a considerable advantage compared to the traditional phenotyping methods currently used to distinguish between the species of the genus. The assay was able to detect approximately 10(3) bacterial cells per gram lung tissue sample, as determined with spiked tissue samples. We assume that the assay could become useful for fast laboratory diagnostic assessment particularly of respiratory infections caused by Mannheimia in animals.     

Monocercus dokuchaevi sp. n. (Cyclophyllidea: Dilepididae)--a new species of cestodes from shrews of the North-Eastern Asia

A new cestode species, Monocercus dokuchaevi sp. n. is described from shrews of the genus Sorex from the Middle Kolyma plateau (Magadan Province). The new species is most closer to M. soricis (Neiland, 1953) by having regular alteration of genital atriums in the short strobila (up to 10 mm only). The size of rostellar hooks in M. dokuchaevi is intermedial between those in M. soricis and M. arioni (Sibold, 1850). In Monocercus dokuchaevi, the length of rostellar hooks is 0.038-0.045 mm (average 0.045 mm), in M. arioni, average is 0.05 mm, in M. soricis, limits are 0.027-0.033 mm. Two other species of the genus, M. baicalensis Eltyshev, 1971 and M. estavarensis Euzet et Jourdan, 1968, have much longer rostellar hooks, 0.07-0.08 and 0.092-0.106 mm, respectively, whereas the genital atriums in these species are altered irregularly.     

Revision of the Indo–Australian parasitic wasp genus Macrobracon with the description of two new species (Hymenoptera: Braconidae)

Illustrated keys to males and females of the 13 known species of the Indo-Australian braconine genus Macrobracon Szépligeti are provided. Principal components analysis has been conducted to aid in the recognition. All known species are redescribed. Two new species are described: M. flavus from Malaysia and Indonesia and M. unicolor from Taiwan.     

An evaluation of the monophyly of Massarina based on ribosomal DNA sequences

The monophyletic status of the genus Massarina was evaluated on the basis of phylogenetic analysis of the partial small subunit gene (SSU), internal transcribed spacers (ITS 1 & 2), and 5.8S gene sequences of the ribosomal DNA. Species of Massarina used in the study clustered into two distinct clades with high bootstrap support in trees generated from maximum parsimony, weighted parsimony, maximum likelihood, and neighbor-joining analyses. The hypothesis that Massarina species belong to a phylogenetically monophyletic group is rejected. Species with narrowly fusiform ascospores form a monophyletic clade with Lophiostoma, a genus highly similar in morphology. The five species currently accepted in Massarina with such spore morphology are here transferred into the genus Lophiostoma. Massarina species with broadly fusiform to ellipsoidal ascospores are retained as Massarina s. str., lectotypified by M. eburnea. Massarina walkeri is presently excluded from both Massarina and Lophiostoma. The transfer of M. papulosa to a new genus Oletheriostrigula is verified.     

A Preliminary Study on the Taxonomy of the Family Magnoliaceae

A new system of classification of Magnoliaceae proposed. This paper deals mainly with taxonomy and phytogeography of the family Magnoliaceae on the basis of external morphology, wood anatomy and palynology. Different authors have had different ideas about the delimitation of genera of this family, their controversy being carried on through more than one hundred years (Table I). Since I have been engaged in the work of the Flora Reipublicae Popularis Sinicae, I have accumulated a considerable amount of information and material and have investigated the living plants at their natural localities, which enable me to find out the evolutionary tendencies and primitive morphological characters of various genera of the family. According to the evolutionary tendencies of the characters and the geographical distribution of this family I propose a new system by dividing it into two subfamilies, Magnolioideae and Liriodendroideae Law (1979), two tribes, Magnolieae and Michelieae Law, four subtribes, Manglietiinae Law, Magnoliinae, Elmerrilliinae Law and Micheliinae, and fifteen genera (Fig. 1 ), a system which is different from those by J. D. Dandy (1964-1974) and the other authors. The recent distribution and possible survival centre of Magnoliaceae. The members of Magnoliaceae are distributed chiefly in temperate and tropical zones of the Northern Hemisphere, ——Southeast Asia and southeast North America, but a few genera and species also occur in the Malay Archipelago and Brazil of the Southern Hemisphere. Forty species of 4 genera occur in America, among which one genus (Dugendiodendron) is endemic to the continent, while about 200 species of 14 genera occur in Southeast Asia, of which 12 genera are endemic. In China there are about 110 species of 11 genera which mostly occur in Guangxi, Guangdong and Yunnan; 58 species and more than 9 genera occur in the mountainous districts of Yunnan. Moreover, one genus (Manglietiastrum Law, 1979) and 19 species are endemic to this region. The family in discussion is much limited to or interruptedly distributed in the mountainous regions of Guangxi, Guangdong and Yunnan. The regions are found to have a great abundance of species, and the members of the relatively primitive taxa are also much more there than in the other regions of the world. The major genera, Manglietia, Magnolia and Michelia, possess 160 out of a total of 240 species in the whole family. Talauma has 40 species, while the other eleven genera each contain only 2 to 7 species, even with one monotypic genus. These three major genera are sufficient for indicating the evolutionary tendency and geographical distribution of Magnoliaceae. It is worthwhile discussing their morphological characters and distributional patterns as follows: The members of Manglietia are all evergreen trees, with flowers terminal, anthers dehiscing introrsely, filaments very short and flat, ovules 4 or more per carpel. This is considered as the most primitive genus in subtribe Manglietiinae. Eighteen out of a total of 35 species of the genus are distributed in the western, southwest to southeast Yunnan. Very primitive species, such as Manglietia hookeri, M. insignis and M. megaphylla, M. grandis, also occur in this region. They are distributed from Yunnan eastwards to Zhejiang and Fujian through central China, south China, with only one species (Manglietia microtricha) of the genus westwards to Xizang. There are several species distributing southwards from northeast India to the Malay Archipelago (Fig. 7). The members of Magnolia are evergreen and deciduous trees or shrubs, with flowers terminal, anthers dehiscing introrsely or laterally, ovules 2 per carpel, stipule adnate to the petiole. The genus Magnolia is the most primitive in the subtribe Magnoliinae and is the largest genus of the family Magnoliaceae. Its deciduous species are distributed from Yunnan north-eastwards to Korea and Japan (Kurile N. 46’) through Central China, North China and westwards to Burma, the eastern Himalayas and northeast India. The evergreen species are distributed from northeast Yunnan (China) to the Malay Archipelago. In China there are 23 species, of which 15 seem to be very primitive, e.g. Magnolia henryi, M. delavayi, M. officinalis and M. rostrata, which occur in Guangxi, Guangdong and Yunnan. The members of Michelia are evergreen trees or shrubs, with flowers axillary, anthers dehiscing laterally or sublaterally, gynoecium stipitate, carpels numerous or few. Michelia is considered to be the most primitive in the subtribe Micheliinae, and is to the second largest genus of the family. About 23 out of a total of 50 species of this genus are very primitive, e.g. Michelia sphaerantha, M. lacei, M. champaca, and M. flavidiflora, which occur in Guangdong, Guangxi and Yunnan (the distributional center of the family under discussion) and extend eastwards to Taiwan of China, southern Japan through central China, southwards to the Malay Archipelago through Indo-China. westwards to Xizang of China, and south-westwards to India and Sri Lanka (Fig. 7). The members of Magnoliaceae are concentrated in Guangxi, Guangdong and Yunnan and radiate from there. The farther away from the centre, the less members we are able to find, but the more advanced they are in morphology. In this old geographical centre there are more primitive species, more endemics and more monotypic genera. Thus it is reasonable to assume that the region of Guangxi, Guangdong and Yunnan, China, is not only the centre of recent distribution, but also the chief survival centreof Magnoliaceae in the world.