THE RESPIRATION OF TOBACCO LEAVES AFTER SYSTEMIC INFECTION WITH TOBACCO MOSAIC VIRUS

The rate of CO, production per g. dry matter of the younger leaves of tobacco plants systemically infected with tobacco mosaic virus was about 10 yo less than that of comparable healthy leaves. Older infected leaves, showing well-developed mosaic symptoms, had the same respiration rate as comparable healthy leaves. These results were independent of seasonal change in light conditions during the growth of the plants. Older leaves, but not younger leaves, of infected plants had a lower initial water content, and both absorbed less water during the experimental period, than leaves from healthy plants. The effects of TMV infection on water content were so great that the rate of CO, production per g. fresh weight was sometimes significantly increased by infection. This reversal of the apparent effect of infection on respiration rate, depending on the basis of reference may partly account for contradictory results reported previously by other workers. Other causes for contradictory results are discussed.     

THE RESPIRATION OF TOBACCO LEAVES IN THE 20-HOUR PERIOD FOLLOWING INOCULATION WITH TOBACCO MOSAIC VIRUS

The effect of infection with tobacco mosaic virus on the respiration rates of detached tobacco leaves in the period immediately after inoculation differed in plants grown at different times of the year. During winter, infection increased respiration rates, and in summer decreased them. In winter-grown plants, increasing the light intensity during the period before inoculation decreased respiration rates after infection. Extending the day length for winter-grown plants did not alter the effect of infection on respiration. Respiration rates began to change in less than 1 hr. after inoculation and are unlikely to be associated with the formation of new virus.     

THE EFFECT OF INFECTION WITH TOBACCO ETCH VIRUS ON THE RATES OF RESPIRATION AND PHOTOSYNTHESIS OF TOBACCO LEAVES

Unlike tobacco mosaic virus, which increases the respiration of tobacco leaves within an hour of their being inoculated, a virulent strain of tobacco etch virus did not change respiration rates until leaves showed external symptoms. The respiration rates of inoculated or systemically infected leaves with symptoms rose to 40% above that of healthy leaves, three times the increase produced by tobacco mosaic virus. The increased respiration rate occurred at all times of the year and was maintained through the life of the leaves.
Leaves infected with tobacco etch virus and showing symptoms had a photo-synthetic rate 20% lower than that of healthy leaves.     

THE EFFECTS OF INFECTION WITH TOBACCO MOSAIC VIRUS ON THE PHOTOSYNTHESIS OF TOBACCO LEAVES

The rate of photosynthesis of tobacco leaves infected with the Rothamsted type culture of tobacco mosaic virus was lower than that of comparable healthy tobacco leaves. The lower rate was inferred from Net Assimilation Rates of whole plants and confirmed by direct comparisons of photosynthetic rates of inoculated and healthy leaves. The effect began within 1 hr. of inoculation. It was not caused by an effect of the virus on the stomata, and inactivated virus inoculum did not change the rates. The results indicate either a more rapid movement of virus from the epidermis into the chlorenchyma than has been previously recorded or an effect of virus infection at a site distant from the cells containing virus.     

THE INOCULATION OF TOBACCO CALLUS TISSUE WITH TOBACCO MOSAIC VIRUS

Although cultures of normal and conditioned tobacco callus tissue occasionally became infected when dilute solutions of tobacco mosaic virus was poured over them, injuries were usually required, and the number of infections depended on the type and number of injuries. Tissues infected by superficial injuries usually became virus-free after subculturing, whereas those infected by needle-prick remained infected permanently. Although no plasmodesmata were found joining cells in the tissue cultures, tobacco mosaic virus moved between them at a rate of about 1 mm. per week, approximately the same rate as it moves through cells of the leaf parenchyma.     

PHOTOSYNTHESIS AND RESPIRATION RATES OF LEAVES OF NICOTIANA GLUTINOSA INFECTED WITH TOBACCO MOSAIC VIRUS AND OF N. TABACUM INFECTED WITH POTATO VIRUS X

The rates of respiration and of photosynthesis of tobacco leaves infected with potato virus X were not affected until the leaves showed symptoms; the respiration rate was then increased by more than 30% and the photosynthesis rate decreased by 20%. When local lesions appeared on the leaves of Nicotiana glutinosa infected with tobacco mosaic virus, but not before, the respiration rate was increased by an amount, up to 30%, that varied with the number of lesions. The photosynthesis rate was decreased by 20%, but there was no effect on photosynthesis or respiration until symptoms appeared. These results differ from those previously reported for tobacco leaves infected with tobacco mosaic virus, in which both respiration and photosynthesis were affected within 1 hr. of inoculation. The validity of extrapolating arguments based on the results obtained with other combinations to this commonly used combination and vice-versa is questioned.     

THE EFFECT OF TEMPERATURE ON INFECTION WITH STRAINS OF CUCUMBER MOSAIC VIRUS

Cucumber mosaic virus strains differed in their ability to multiply in plants at 37° C. Some strains multiplied in inoculated leaves and produced systemic symptoms in plants at this temperature; plants systemically infected with one such strain remained infected after prolonged treatment at 37° C. Other strains did not appear to multiply in inoculated leaves at 37° C. and heat treatment was successful in freeing plants from infection with these. Tests with one strain of each type showed both to be rapidly inactivated in expressed sap at 37° C.
Strains of cucumber mosaic virus forming small necrotic local lesions in leaves of french bean var. Canadian Wonder, produced many fewer lesions in plants kept after inoculation at 25° C. for 24 hr. and then at 15° C. than in plants kept continuously at the lower temperature.     

EFFECTS OF DARKNESS ON THE CONSTITUTION OF TOBACCO LEAVES AND SUSCEPTIBILITY TO VIRUS INFECTION

An attempt was made to find the causes of increased susceptibility to virus infection when tobacco plants are kept in the dark before inoculation. The changes in certain nitrogen fractions, viz. insoluble-N, amino-N, amide-N, ammonia-N and nitrate-N, and in dry matter and water content were followed in tobacco plants subjected to a period of darkness before inoculation with tobacco aucuba mosaic virus. Only nitrate-N was strongly correlated with the susceptibility to infection, but the evidence suggests that the correlation is indirect and not causal.
Dry matter and water content, determined either as dry matter percentage of fresh weight or measured separately on a leaf-disk basis Ivere found to vary directly with variation in susceptibility.     

THE EFFECT OF INFECTION WITH BEET YELLOWS AND BEET MOSAIC VIRUSES ON THE CARBOHYDRATE CONTENT OF SUGAR-BEET LEAVES, AND ON TRANSLOCATION

The loss of total carbohydrate (sugars and starch) per cent of residual dry matter (dry matter less total carbohydrate) during a period of darkness from leaves of sugar-beet plants infected with yellows virus was as great as that from the leaves of healthy plants. The conclusion of previous workers, based on the results of the Sachs iodine test for starch and the occurrence of 'phloem gummosis' in infected plants, that starch accumulates in infected leaves because translocation is prevented by blockage of the sieve-tubes, is therefore incorrect.
Older leaves of infected plants had a higher content of reducing sugars and sucrose, and usually but not invariably of starch, both at the beginning and end of the dark period, than comparable leaves of healthy plants. By far the greater part of the increase was in reducing sugars. In leaves taken in late September from infected plants growing in the field, 20 % or more of the total dry matter was present as reducing sugars. The reducing sugars in both healthy and yellows-infected leaves were shown by paper chromatography to be glucose and fructose in approximately equal amounts.
Accumulation of carbohydrate in infected leaves is probably not a passive consequence of differences in carbohydrate production, distribution and utilization, but is attributable to changes in the physiology of the cells of the leaf.
The carbohydrate content of sugar-beet leaves was little affected by infection with beet mosaic virus.
Yellows-infected leaves had a lower water content per cent of fresh weight than healthy leaves. This was accounted for by the higher carbohydrate content of infected leaves, for the ratio of water: residual dry matter was not affected by infection or was slightly reduced. This implies that hydration was independent of carbohydrate content.     

EFFECTS OF TOBACCO MOSAIC VIRUS ON EARLY LEAF DEVELOPMENT IN TOBACCO

Tepfer , Sanford S. (U. Oregon, Eugene.), and Meyer Chessin . Effects of tobacco mosaic virus on early leaf development in tobacco. Amer. Jour. Bot. 46(7): 496–509. Illus. 1959.—At certain stages of infection, tobacco mosaic virus causes the formation of highly abnormal tobacco leaves classified here as narrow-bladed leaves and “shoestring” leaves. The development and anatomy of these types are described. The “shoestring” leaf in its extreme form is entirely radial in symmetry, with no vestige of a lamina. This suppression of dorsiventrality is expressed to a lesser degree in narrow-bladed leaves and in transitional forms. The lack of or reduction of dorsiventrality results directly from the absence of or reduced activity of the marginal meristems of the leaf primordium. There is a general reduction in meristematic activity in the primordium that causes reduced length as well as reduced laminal development.     

CYTOLOGICAL AND CYTOCHEMICAL OBSERVATIONS ON THE EARLY STAGE OF INFECTION OF TOMATO HAIR CELLS BY TOBACCO MOSAIC VIRUS

Strips of epidermal tissue bearing hair cells were removed fromtomato stems. The lower surface exposed by the stripping wasinoculated with tobacco mosaic virus and the progress of infectionof the hair cells was followed microscopically and by extractionof infectivity. Shortly before infectivity could be extractedfrom the hair cells, the nuclei were observed to have migratedtowards the tran sverse wall of the basal hair cell nearestthe epidermis, apparently the site at which infection of thecell commences. After infection was apparent, the staining characteristicsof the nuclei, before and after RNase and DNase treatment, suggestedan accumulation of RNA. A granular body, different from X-bodiesmaintained a close association with the nucleus of TMV infectedhair cells, and was found to be rich in RNA, suggesting thatviral RNA of the nucleus may have been entering the cytoplasmvia the granular body. A possible mechanism of X-body formationarising from the granular body is suggested. ¹ Supported in part by a grant E-536 (C9) from the U. S. PublicHealth Service. ² Present address: Plant Pathology Laboratory, Faculty of Agriculture,Nagoya University, Anjo, Aichi (Japan). (Received March 4, 1963; )     

TOBACCO MOSAIC VIRUS IN CHLOROPLAST AND CYTOPLASM OF INFECTED TOBACCO LEAF

Chloroplasts containing Tobacco Mosaic Virus (TMV) were isolatedfrom TMV inoculated tobacco leaves, and TMV was extracted fromthem. The preparations from isolated chloroplasts of infectedleaves showed 30% increase in the optical density at 260 mµover those from healthy leaves. Most of infectivity was observedin chloroplast fraction 40 hr after inoculation. Thereafter,infectivity in the chloroplast fraction decreased and that ofcytoplasm increased with time. (Received April 6, 1964; )     

类根瘤对烟草叶片中叶绿体及其淀粉粒含量的影响

目的：探讨类根瘤对烟草叶片中的叶绿体及其淀粉粒含量的影响。方法：无菌培养烟草再生植株,其中一部分经过一定浓度的2,4－D与豇豆根瘤菌512快生型突变株菌液诱导处理,另外一部分为对照。运用常规电镜技术制作叶片厚切片,通过显微观察,对两组烟草叶片细胞中的叶绿体及其淀粉粒含量进行对比分析。结果：结瘤植株叶片细胞内的叶绿体含量虽然与对照之间无明显差异,但叶片栅栏组织和海绵组织细胞内淀粉粒的含量却均较对照减少,其中前者减少更为明显（P＜0.05)。结论：结瘤烟草叶片内光合产物的大量同化可能与类根瘤的形成有关。     

表达黄瓜花叶病毒卫星RNA的转基因烟草耐烟草花叶病毒

表达黄瓜花叶病毒(CMV)的卫星RNA的转基因烟草可以抗CMV的侵染.为了决定这些植物对其它病毒,如烟草花叶病毒(CMV)的安全性,我们对这些植物接种CMV.结果发现 卫星RNA可减弱TMV引起的症状,井降低病情指数,然而却对TMV在植物中的积累没有明显影响.这一发现有助于对卫星RNA抗病机制的理解,有助于含卫星RNA的生防制剂及表达病毒卫星RNA的转基因植物的应用.本文在国际上首次报道卫星RNA可减弱非相关病毒引起的症状.1 材料和方法1.1 病毒及植物烟草花叶病毒(TMV),烟草G140种子及枯班三生烟种子均为中国科学院微生物研究所病毒室保存;含黄瓜花叶病毒卫星RNA-R1基因的烟草G140(烟Sat-G140)为中国科学院微生物研究所病毒室培育,所用材料为第三代种子.