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1.
On the function of repetitive domains in protein antigens of Plasmodium and other eukaryotic parasites 总被引:10,自引:0,他引:10
Schofield L 《Parasitology today (Personal ed.)》1991,7(5):99-105
Highly reiterated repetitive domains occur within the protein antigens of many parasitic taxa, including Plasmodium, Trypanosoma, Leishmania and Toxoplasma. In malaria it has been proposed that repeat regions may function as ligands for host proteins, or serve to suppress the development of immunity through a strategy of serological crossreactivity. In this article Louis Schofield presents a novel hypothesis, based on empirical evidence, that repetitive domains in antigens do not elicit protective immune responses and instead have evolved as a mechanism of immune evasion by their ability to induce thymus-independent B-cell activation. It is also proposed that this unusual response is associated with several forms of immunosuppression. The hypothesis has the added attraction of helping to explain several distinctive features of the molecular biology, evolution and immunology of repetitive regions in protein antigens of parasites. 相似文献
2.
A. Desiree LaBeaud Indu Malhotra Maria J. King Christopher L. King Charles H. King 《PLoS neglected tropical diseases》2009,3(5)
On a global basis, both potent vaccine efficacy and high vaccine coverage are necessary to control and eliminate vaccine-preventable diseases. Emerging evidence from animal and human studies suggest that neglected tropical diseases (NTDs) significantly impair response to standard childhood immunizations. A review of efficacy and effectiveness studies of vaccination among individuals with chronic parasitic infections was conducted, using PUBMED database searches and analysis of data from the authors'' published and unpublished studies. Both animal models and human studies suggest that chronic trematode, nematode, and protozoan infections can result in decreased vaccine efficacy. Among pregnant women, who in developing countries are often infected with multiple parasites, soluble parasite antigens have been shown to cross the placenta and prime or tolerize fetal immune responses. As a result, antenatal infections can have a significant impact on later vaccine responses. Acquired childhood parasitic infections, most commonly malaria, can also affect subsequent immune response to vaccination. Additional data suggest that antiparasite therapy can improve the effectiveness of several human vaccines. Emerging evidence demonstrates that both antenatal and childhood parasitic infections alter levels of protective immune response to routine vaccinations. Successful antiparasite treatment may prevent immunomodulation caused by parasitic antigens during pregnancy and early childhood and may improve vaccine efficacy. Future research should highlight the varied effects that different parasites (alone and in combination) can have on human vaccine-related immunity. To optimize vaccine effectiveness in developing countries, better control of chronic NTDs may prove imperative. 相似文献
3.
Parasites impose different selection regimes on their hosts, which respond by increasing their resistance and/or tolerance. Parental challenge with parasites can enhance the immune response of their offspring, a phenomenon documented in invertebrates and termed transgenerational immune priming. We exposed two parental generations of the model organism Daphnia magna to the horizontally transmitted parasitic yeast Metschnikowia bicuspidata and recorded resistance- and tolerance-related traits in the offspring generation. We hypothesized that parentally primed offspring will increase either their resistance or their tolerance to the parasite. Our susceptibility assays revealed no impact of parental exposure on offspring resistance. Nonetheless, different fitness-related traits, which are indicative of tolerance, were altered. Specifically, maternal priming increased offspring production and decreased survival. Grandmaternal priming positively affected age at first reproduction and negatively affected brood size at first reproduction. Interestingly, both maternal and grandmaternal priming significantly reduced within-host–parasite proliferation. Nevertheless, Daphnia primed for two consecutive generations had no competitive advantage in comparison to unprimed ones, implying additive maternal and grandmaternal effects. Our findings do not support evidence of transgenerational immune priming from bacterial infections in the same host species, thus, emphasizing that transgenerational immune responses may not be consistent even within the same host species. 相似文献
4.
Cell-mediated immunity (CMI) is important in many parasitic infections. Stimulation by parasite-specific antigens can induce clonal expansion of parasite-specific T-cells which may act by direct cytotoxic action or by indirect effects on other cells such as natural killer cells or antibody-producing B-cells (Box 1). In many cases however, the precise effector functions and the identity of antigens that elicit protective responses in parasitic infections are poorly defined. Analysis of proliferative and cytotoxic activities of subcloned cultures of T-cells stimulated with parasite antigens can provide some clues about the importance of CMI, but, as this review shows, much more precise information can be obtained by analysis of the response of cloned T-cell cultures. 相似文献
5.
During natural infections of P. vivax malaria a variety of immune responses to the infection affect infectivity of the parasites to mosquitoes. Sexual stage antigens present in the blood stage parasites induce antibodies which may either enhance or suppress the infectivity of the sexual parasites to mosquitoes. Subsequent infections of P. vivax do not, unless occurring within less than 4 months, boost this response indicating a very short immune memory for the relevant antigens. Blood infection also results in the release of cytokines and other non-antibody factors which together can mediate death of the blood stage sexual parasites. These factors are associated with paroxysm in non-immune individuals. In individuals from an endemic area with age-acquired anti-disease immunity clinical symptoms are mild and the parasite killing factors are not induced. 相似文献
6.
Malaria can be a very severe disease, particularly in young children, pregnant women (mostly in primipara), and malaria na?ve adults, and currently ranks among the most prevalent infections in tropical and subtropical areas throughout the world. The widespread occurrence and the increased incidence of malaria in many countries, caused by drug-resistant parasites (Plasmodium falciparum and P. vivax) and insecticide-resistant vectors (Anopheles mosquitoes), indicate the need to develop new methods of controlling this disease. Experimental vaccination with irradiated sporozoites can protect animals and humans against the disease, demonstrating the feasibility of developing an effective malaria vaccine. However, developing a universally effective, long lasting vaccine against this parasitic disease has been a difficult task, due to several problems. One difficulty stems from the complexity of the parasite's life cycle. During their life cycle, malaria parasites change their residence within the host, thus avoiding being re-exposed to the same immunological environment. These parasites also possess some distinct antigens, present at different life stages of the parasite, the so-called stage-specific antigens. While some of the stage-specific antigens can induce protective immune responses in the host, these responses are usually genetically restricted, this being another reason for delaying the development of a universally effective vaccine. The stage-specific antigens must be used as immunogens and introduced into the host by using a delivery system that should efficiently induce protective responses against the respective stages. Here we review several research approaches aimed at inducing protective anti-malaria immunity, overcoming the difficulties described above. 相似文献
7.
Exploiting natural immunity to helminth parasites for the development of veterinary vaccines 总被引:7,自引:0,他引:7
The development of subunit vaccines against most parasitic helminth infections will require a better understanding of the different components of a natural rejection process including (1) recognition of parasite antigens; (2) induction of protective immune response phenotypes; and (3) activation of appropriate immune effector mechanisms. While novel technologies have allowed significant progress to be made in the identification of candidate vaccine antigens, the large scale production of these antigens and their presentation to the host with appropriate adjuvant systems remains a major problem in vaccine research. Identification of the molecular interactions involved in the innate immune response to helminth infections and the application of new genomic and proteomic technologies are likely to lead to major advances in these research fields. Gastrointestinal nematode parasites and liver fluke are the most important helminth parasites of production animals. In recent years, a lot of new knowledge has been gathered on the immunobiology of the host-parasite interactions in these two infection systems, which has allowed new vaccination strategies to be considered. Functional genomic technologies such as gene expression analysis by microarrays, promise to further advance our understanding of the molecular pathways leading to protection against parasite infections. This will not only have implications for vaccine research, but also provide novel targets for drug development and genetic selection. 相似文献
8.
The costs and benefits of activating the immune system can reach across generations. Thus, in vertebrates and in several invertebrates, stimulating the immune system of a female can enhance immunity of her offspring or decrease offspring fitness. We evaluated the potential maternally transmitted costs and benefits of the melanization response, an innate immune response of insects that helps to protect mosquitoes from malaria parasites. We manipulated the maternal melanization response of the yellow fever mosquito Aedes aegypti by inoculating female mosquitoes with negatively charged sephadex beads or with immunologically inert glass beads; a control group was not inoculated. In the next generation, we assayed the melanization response and measured three other life-history traits: survival up to emergence, the age at emergence, and body size (estimated as wing length). We found no evidence of fitness costs or benefits for the offspring. A retrospective power analysis found that our experiment would have detected an effect size that is three times smaller than the maternal immune priming effects that have been reported in the literature. We did find a strong correlation between offspring wing length and melanization response. Overall, our findings indicate that trans-generational immune priming in invertebrates cannot be generalized, and that it may depend on the species, the immune challenge, and the environmental conditions. 相似文献
9.
Transgenerational priming of immunity: maternal exposure to a bacterial antigen enhances offspring humoral immunity 总被引:2,自引:0,他引:2
Grindstaff JL Hasselquist D Nilsson JK Sandell M Smith HG Stjernman M 《Proceedings. Biological sciences / The Royal Society》2006,273(1600):2551-2557
Young vertebrates have limited capacity to synthesize antibodies and are dependent on the protection of maternally transmitted antibodies for humoral disease resistance early in life. However, mothers may enhance fitness by priming their offspring's immune systems to elevate disease resistance. Transgenerational induced defences have been documented in plants and invertebrates, but maternal priming of offspring immunity in vertebrates has been essentially neglected. To test the ability of mothers to stimulate the immune systems of offspring, we manipulated maternal and offspring antigen exposure in a wild population of birds, pied flycatchers (Ficedula hypoleuca). We show that immunization of the mother before egg laying apparently stimulates a transgenerational defence against pathogens by elevating endogenous offspring antibody production. If the disease environments encountered by mothers and offspring are similar, this transgenerational immune priming may allow young to better cope with the local pathogen fauna. 相似文献
10.
Bas ter Braak Alice M. Laughton Boran Altincicek Benjamin J. Parker Nicole M. Gerardo 《PloS one》2013,8(8)
Pea aphids have an obligate nutritional symbiosis with the bacteria
Buchnera
aphidicola
and frequently also harbor one or more facultative symbionts. Aphids are also susceptible to bacterial pathogen infections, and it has been suggested that aphids have a limited immune response towards such pathogen infections compared to other, more well-studied insects. However, aphids do possess at least some of the genes known to be involved in bacterial immune responses in other insects, and immune-competent hemocytes. One possibility is that immune priming with microbial elicitors could stimulate immune protection against subsequent bacterial infections, as has been observed in several other insect systems. To address this hypothesis we challenged aphids with bacterial immune elicitors twenty-four hours prior to live bacterial pathogen infections and then compared their survival rates to aphids that were not pre-exposed to bacterial signals. Using two aphid genotypes, we found no evidence for immune protection conferred by immune priming during infections with either Serratia marcescens or with Escherichia coli. Immune priming was not altered by the presence of facultative, beneficial symbionts in the aphids. In the absence of inducible immune protection, aphids may allocate energy towards other defense traits, including production of offspring with wings that could escape deteriorating conditions. To test this, we monitored the ratio of winged to unwinged offspring produced by adult mothers of a single clone that had been exposed to bacterial immune elicitors, to live E. coli infections or to no challenge. We found no correlation between immune challenge and winged offspring production, suggesting that this mechanism of defense, which functions upon exposure to fungal pathogens, is not central to aphid responses to bacterial infections. 相似文献
11.
Newborn animals do not have a fully functional immune system and are thus impaired in their ability to fight parasites. Mothers can therefore increase the survival probability of their young by providing them with passive immunity, e.g. in the form of maternal antibodies transferred via the placenta or the eggs. The maternal responses are only induced when parasites are present, and have been observed not only in vertebrates but also in invertebrates. However, while these parasite-induced maternal effects are known to reduce the harmful effects of common parasites, they may also impose costs for the young, either because the maternal response impairs parental performance, or because maternally transmitted products moderate offspring development. We experimentally tested these two hypotheses in a wild great tit population. We exposed birds to a common ectoparasite before egg-laying to induce the maternal response, and thereafter separated egg-mediated maternal effects from effects on post-laying parental performance by cross-fostering whole clutches. To assess the costs of this response without its confounding benefits, we kept nests free of parasites after hatching. Since the costs of maternal effects can be expressed differently under relaxed and harsh rearing conditions, we simultaneously manipulated clutch size. First, parasite-exposed parents raised lighter young, suggesting that parasite defence or the induced maternal response are costly to the parents and reduce their capacity to raise young. Second, under relaxed but not under harsh rearing conditions, young with the flea-induced maternal effect were heavier and were in better body condition than controls, suggesting that the maternally transferred products can be allocated to physiological functions beyond parasite defence. 相似文献
12.
Within the last decade bacterial plasmids encoding foreign antigens have revolutionized vaccine design. Although no DNA vaccine has yet been approved for routine human or veterinary use, the potential of this vaccine modality has been demonstrated in experimental animal models. Plasmid DNA vaccination has shown efficacy against viral, bacterial and parasitic infections, modulated the effects of autoimmune and allergic diseases and induced control over cancer progression. With a better understanding of the basic immune mechanisms that govern induction of protective or curative immune responses, plasmid DNA vaccines and their mode of delivery are continuously being optimized. Because of the simplicity and versatility of these vaccines, various routes and modes of delivery are possible to engage the desired immune responses. These may be T or B effector cell responses able to eliminate infectious agents or transformed cells. DNA vaccines may also induce an immunoregulatory/modulatory or immunosuppressive (tolerizing) response that interferes with the differentiation, expansion or effector functions of B and T cells. In this sense a DNA vaccine may be thought of as a 'negative' vaccine. Pre-clinical and initial small-scale clinical trials have shown DNA vaccines in either of these modes to be safe and well tolerated. Although DNA vaccines induce significant immune responses in small animal trials their efficacy in humans has so far been less promising thus necessitating additional optimizations of this novel vaccine approach. 相似文献
13.
Staszewski V Gasparini J McCoy KD Tveraa T Boulinier T 《The Journal of animal ecology》2007,76(6):1215-1223
Little is known about the maternal transfer of antibodies in natural host-parasite systems despite its possible evolutionary and ecological implications. In domestic animals, the maternal transfer of antibodies can enhance offspring survival via a temporary protection against parasites, but it can also interfere with the juvenile immune response to antigens. We tested the functional role of maternal antibodies in a natural population of a long-lived colonial seabird, the kittiwake (Rissa tridactyla), using a vaccine (Newcastle disease virus vaccine) to mimic parasite exposure combined with a cross-fostering design. We first investigated the role of prior maternal exposure on the interannual transmission of Ab to juveniles. We then tested the effect of these antibodies on the juvenile immune response to the same antigen. The results show that specific maternal antibodies were transferred to chicks 1 year after maternal exposure and that these antibodies were functional, i.e. they affected juvenile immunity. These results suggest that the role of maternal antibodies may depend on the timing and pattern of offspring exposure to parasites, along with the patterns of maternal exposure and the dynamics of her immune response. Overall, our approach underlines that although the transgenerational transfer of antibodies in natural populations is likely to have broad implications, the nature of these effects may vary dramatically among host-parasite systems, depending on the physiological mechanisms involved and the ecological context. 相似文献
14.
P Cibrelus E Précigout D Sereno B Carcy J L Lemesre A Gorenflot 《Parasite (Paris, France)》1999,6(2):121-129
To study the antigens secreted by promastigote and amastigote forms of Leishmania infantum which are able to induce a humoral response in human patients and dogs, we have carried out immunoprecipitation assays with different supernatants of in vitro cultured parasites, metabolically labelled with [35S]methionine, using serum samples from human patients and dogs. In addition, some metabolic labelling experiments were performed daily during the in vitro culture parasite's life cycle to follow the time course excretion-secretion of parasitic antigens. The results demonstrated that the two different hosts developed an antibody response against secreted antigens of both stages of Leishmania infantum. Nevertheless, the humoral response directed against the excreted-secreted antigens of the promastigote forms was qualitatively and quantitatively different when we compare the human and the dog immune responses. On the other hand, when the excreted-secreted antigens of the amastigote forms are immunoprecipitated with either human or canine immune serum, the humoral response is similar. In addition, the time course study showed that excretion-secretion of antigens was qualitatively and quantitatively modulated during the parasitic in vitro life cycle. 相似文献
15.
Mucosae constitute the major entry for most microbial pathogens but also innocuous antigens derived from ingested food, airborne matter or commensal bacteria. A large and highly specialized innate and adaptative mucosal immune system protects the mucosal surfaces and the body interior from potential injuries from the environment. The mucosal immune system has developed a variety of immune mechanisms to discriminate between non-pathogenic and pathogenic invaders. It is able to maintain tolerance against the plethora of environmental antigens and to induce potent protective immunity to avoid mucosal colonisation and organism invasion by dangerous microbial pathogens. Mucosal immunisation with appropriate antigens and immunostimulatory molecules may induce potent protective immunity against harmful pathogens. Alternatively, mucosally-induced tolerance against auto-antigens or allergens may be generated by mucosal administration of these antigens alone or with immunomodulators potentiating regulatory responses. Here, we review the properties of the mucosal immune system and briefly discuss the advances in the development of mucosal vaccines for protection against infections and for the treatment of inflammatory disorders such as autoimmune diseases or type I allergies. 相似文献
16.
Ayash-Rashkovsky M Weisman Z Zlotnikov S Raz E Bentwich Z Borkow G 《Biochemical and biophysical research communications》2001,282(5):1169-1176
A requisite for vaccines to confer protection against intracellular infections such as Human Immunodeficiency Virus or Mycobacterium tuberculosis is their capacity to induce Th1 immune responses. However, they may fail to do so in Africa and South East Asia, where most individuals have a dominant preexistent Th2 immune profile, due to persistent helminthic parasitic infections, which may undermine any Th1 response. It is well established that DNA vaccines induce strong Th1 biased immune responses against an encoded antigen, depending on the route and mode of immunization. Here, we demonstrate that intradermal immunization with plasmid DNA encoding beta-gal (pCMV-LacZ) of Schistosoma-infected mice, with preexistent dominant Th2 immune background, induce a strong Th1 anti-beta-gal response, as opposed to immunized with beta-gal only. Importantly, the established protective Th2 immune response to schistosomes was not disrupted. These findings strongly support the possibility of using plasmid DNA as a Th1 inducing adjuvant when immunizing populations with a strong preexistent Th2 immune profile. 相似文献
17.
Hasselquist D Nilsson JA 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2009,364(1513):51-60
Maternal effects by which females provide their offspring with non-genetic factors such as hormones, nutrients and antibodies can have an important impact on offspring fitness. In vertebrates, maternal antibodies (matAb) are transferred from the mother, via the placenta, egg yolk or milk during lactation to offspring until they are 2 weeks (birds), 4-10 weeks (rodents) and 9 months (humans) old, respectively. matAb transfer can have direct effects on offspring growth rate in birds and rodents, probably by passively protecting the newborn from common pathogens before their endogenous immune system has matured. Indirect long-term effects of matAb transfer on the offspring's own immunity can be synergistic, if matAb act as antigen templates of the accumulated immunological experience of the mother and educate the newborn's immune system. However, it may also be suppressive if matAb reduce antigen presentation to the newborn resulting in antigen-specific blocking of offspring endogenous immunity. Our aim is to review the mechanisms and direct effects of matAb transfer in vertebrates with an emphasis on birds, outline a framework for research on the long-term effects of matAb on the endogenous immune system of the mature offspring and encourage ecological and evolutionary studies of matAb transfer in non-domesticated animals. 相似文献
18.
Jérôme Moreau Guillaume Martinaud Jean‐Phillipe Troussard Caroline Zanchi Yannick Moret 《Oikos》2012,121(11):1828-1832
Immune‐challenged vertebrate and invertebrate females can transfer immunity to their offspring. This trans‐generational immune priming (TGIP) is beneficial for the offspring if the maternal infection risk persists across generations. However, because immunity is costly, fitness consequences of TGIP have been found in primed offspring. Furthermore, transferring immunity to offspring may be costly for immune‐challenged females who are also carrying the costs of their immune response. A negative relationship between levels of immunity between mothers and offspring might therefore be expected. Consistent with this hypothesis, we show that in the insect, Tenebrio molitor, the magnitude of antibacterial immune response of immune‐challenged females negatively correlates with levels of antibacterial activity of their eggs. This negative relationship was only present in small females that are inherently of lower quality. Furthermore, female body size did not affect immune responsiveness to the challenge, indicating that small females favoured their immunity at the expenses of that of their eggs. 相似文献
19.
Runlin Han 《Microbiology and immunology》2010,54(4):246-253
Plasma lipoproteins (VLDL, LDL, Lp[a] and HDL) function primarily in lipid transport among tissues and organs. However, cumulative evidence suggests that lipoproteins may also prevent bacterial, viral and parasitic infections and are therefore a component of innate immunity. Lipoproteins can also detoxify lipopolysaccharide and lipoteichoic acid. Infections can induce oxidation of LDL, and oxLDL in turn plays important anti‐infective roles and protects against endotoxin‐induced tissue damage. There is also evidence that apo(a) is protective against pathogens. Taken together, the evidence suggests that it might be valuable to introduce the concept that plasma lipoproteins belong in the realm of host immune response. 相似文献
20.
In this review, Masataka Korenoga and Isoo Toda discuss the role of interleukin 5 (IL-5) in immune responses caused by parasitic infections. Recent studies have shown that eosinophilia in parasitic infections is dependent on IL-5. Although eosinophils have been thought to be potent effector cells (from results of experiments, in vitro), their role in protective immunity to parasites is controversial. 相似文献