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1.
Pavlova IV Rysakova MP 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2011,97(12):1297-1308
By plotting cross-correlation histograms differences were found in interaction of conjectural small "interneurons" and large principal cells of the central and basal amygdalar nuclei in negative emotional situations. The network activity of "nterneurons" was higher than in principal cells. "Interneurons" more frequently had excitatory and inhibitory input or output connections with neighbouring cells, latency of their connections with other cells was smaller than in principal neurons. Interaction of "interneurons" and principal cells differed in animals with active and passive behavioural strategy in negative emotional situations. As compared to active animals, in passive rabbits inhibitory connections to "interneurons" from other cells occurred more frequently, excitatory or inhibitory connections from "interneurons" to principal cells appeared more rare. 相似文献
2.
A liquid culture system is described whereby proliferation of haemopoietic stem cells (CFU-S), production of granulocyte precursor cells (CFU-C), and extensive granulopoiesis can be maintained in vetro for several months. Such cultures consist of adherent and non-adherent populations of cells. The adherent population contains phagocytic mononuclear cells, "epithelial" cells, and "giant fat" cells. The latter appear to be particularly important for stem cell maintenance and furthermore there is a strong tendency for maturing granulocytes to selectively cluster in and around areas of "giant fat" cell aggregations. By "feeding" the cultures at weekly intervals, between 10 to 15 "population doublings" of functionally normal CFU-S regularly occurs. Increased "population doublings" may be obtained by feeding twice weekly. The cultures show initially extensive granulopoiesis followed, in a majority of cases, by an accumulation of blast cells. Eventually both blast cells and granulocytes decline and the cultures contain predominantly phagocytic mononuclear cells. Culturing at 33 degrees C leads to the development of a more profuse growth of adherent cells and these cultures show better maintenance of stem cells and increased cell density. When tested for colony stimulating activity (CSA) the cultures were uniformly negative. Addition of exogenous CSA caused a rapid decline in stem cells, reduced granulopoiesis and an accumulation of phagocytic mononuclear cells. 相似文献
3.
Functional heterogeneity of Leu 19"bright"+ and Leu 19"dim"+ lymphokine-activated killer cells 总被引:7,自引:0,他引:7
The functional heterogeneity of human lymphokine-activated killer (LAK) cells was characterized using LAK effector cells generated in vivo during rIL-2 therapy and separated by FACS into Leu 19"bright"+ and Leu 19"dim"+ subsets. The Leu 19"bright"+ subset mediated significantly greater levels of LAK lytic activity against NK-resistant COLO 205 target cells compared to Leu 19"dim"+ effector cells in chromium release assays. Single cell cytotoxicity assays showed that the Leu 19"bright"+ LAK effector cell subset contained a significantly higher percentage of cells capable of binding to and lysing COLO 205 or K562 target cells compared to the Leu 19"dim"+ subset. Furthermore, individual Leu 19"bright"+ LAK effector cells exhibited a more rapid rate of COLO 205 target cell lysis when compared to Leu 19"dim"+ LAK effector cells. In vitro culturing of Leu 19"bright"+ or Leu 19"dim"+ cells from normal donors with 1500 U/ml rIL-2 resulted in significantly greater levels of proliferation and LAK effector activity by Leu 19"bright"+ cells. Furthermore, whereas 86% of normal Leu 19"bright"+ cells maintained a Leu 19"bright"+ phenotype after rIL-2 stimulation, only 24% of Leu 19"dim"+ cells developed a Leu 19"bright"+ phenotype. These data demonstrate that Leu 19"bright"+ LAK cells are significantly more potent effectors than Leu 19"dim"+ cells due to quantitative and qualitative differences in the LAK effector cells contained within these subsets. Furthermore, these data indicate that Leu 19"bright"+ LAK cells that develop during rIL-2 therapy are derived from Leu 19"bright"+ precursor cells. 相似文献
4.
V G Galaktionov O I Morgunov N N Smirnova T V Anfalova 《Biulleten' eksperimental'no? biologii i meditsiny》1975,79(4):80-82
Simultaneous injection of marrow cells previously incubated with "immune" RNA, thymus cells and sheep red cells to X-ray treated mice resulted in a greater accumulation of antibody-forming cells in the spleen, as compared to mice inoculated with intact cells or with those incubated with normal RNA. Preliminary incubation of thymus cells with "immune" RNA did not affect the accumulation of antibody-forming cells. Incubation of peritoneal exudate cells with "immune" or normal RNA failed to influence the mode of accumulation of the antibody-forming cells either. 相似文献
5.
A A Hassoni G A Kerkut R J Walker 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1988,91(2):525-533
1. Intracellular recordings were made from identified neurones in the suboesophageal ganglionic mass of the snail, Helix aspersa. Neurones were classified as either "H" cells, inhibited by acetylcholine or "D" cells, excited by acetylcholine. 2. The actions of levamisole, morantel, pyrantel, amidantel, deacylated amidantel and hycanthone were investigated on these neurones and compared to that of acetylcholine. 3. Levamisole was 10.85 +/- 0.56 times less active than acetylcholine on "H" cells but more than 100 times less active on "D" cells. On "H" cells levamisole had a secondary gradual depolarizing effect which was irreversible and resulted in the loss of cell activity. 4. Morantel and pyrantel were 1.12 +/- 0.13 and 2.56 +/- 0.26 times respectively less active than acetylcholine on "D" cells and 5.16 +/- 0.6 and 3.53 +/- 0.63 times respectively less active than acetylcholine on "H" cells. 5. Amidantel was more than 100 times less active than acetylcholine on both "D" and "H" cells while its deacylated derivative was 26.0 +/- 1.0 and 76.0 +/- 3.25 times respectively less active than acetylcholine on "D" and "H" cells. 6. Hycanthone possessed weak inhibitory effects on "H" cells but also appeared to reduce the duration of acetylcholine inhibitory responses when applied immediately after the acetylcholine response had reached its maximum. 相似文献
6.
小鼠胚胎干细胞分化为血管内皮细胞的永生化研究 总被引:9,自引:0,他引:9
本文探讨了小鼠胚胎干细胞(ES细胞)、诱导分化的血管内皮细胞永生化。在体外培养系统中,以维甲酸(RA)和转化生长因子-β1(TGF-β1)诱导小鼠胚胎干细胞(ES细胞)的拟胚体(EB)分化为“圆形细胞”和由这些“圆形细胞”组成的血管样结构。经光学和扫描电镜及免疫荧光等法分析检测,证明组成血管样结构的细胞具有专一性vWF荧光染色,表明是血管内皮样细胞。利用脂质体将人端粒酶催化亚基逆转录酶(hTERT)基因转染诱导分化中的“圆形细胞”。应用Dot-blot,RT-PCR,Western blot及免疫组织化学等方法分析、观察和证明了诱导分化的组成血管样结构的园形细胞和被hTERT基因转染的“圆形”细胞的形态和生物学特性。结果表明,携带hTERT基因的从ES细胞分化来的圆形细胞在体外可大量增殖,持续传代,95%具有血管内皮细胞的一些特有标志和管道化生长特征。因此,通过人端粒酶基因的转染途径可解决由ES细胞诱导分化而来的内皮细胞扩增和永生化问题,为构建组织工程化血管及其它人工血管的内皮化提供种子细胞来源打下基础。 相似文献
7.
W H Arnold 《The Histochemical journal》1984,16(7):755-769
Summary Various organs of three species of monkey were screened histochemically for esteroproteases usingN-acethyl-l-methionine--naphthylester ( N-O-met) as the substrate and also for enzymes with kallikrein-like activity usingd-Val-Leu-Arg-4-methoxy-2-naphthylamide as the substrate. Characteristic differences were found in the localization of the reaction products obtained with both substrates. In the main salivary glands, esteroproteases ( N-O-met reactivity) were found in mucous cells (submandibular gland), intercalated duct cells (parotid gland), acinar cells (sublingual gland), striated and interlobular duct cells (all glands). They were also localized in superficial lining epithelial cells of the digestive system, in liver cells, and acinar cells of the pancreas.Enzymes with kallikrein-like activity were found only in the striated and interlobular duct cells of salivary glands, in acinar cells of the pancreas, and in proximal tubular cells of the kidney. Free cells (including mast cells) normally distributed in the connective tissue of various organs showed reactivity towards N-O-met. Some of these cells were also reactive against Val-Leu-Arg-4-MNA. 相似文献
8.
Iu S Balashov 《Parazitologiia》1985,19(5):365-369
Electron microscope observations were conducted to study changes in the cellular structure of IIId type alveoli of feeding females of I. persulcatus. Within the first 1-2 days of feeding granulosecreting glandular cells "d" and "e" get free of secretory inclusions. After that cells "d" degenerate completely and cells "e" undergo morphological transformations and start to fulfil osmoregulatory functions. From the third day of feeding cytoplasm of cells "e" and basal intersticial cells form a complex labyrinth from a system of branching processes limiting intercellular space from the basal to apical surfaces of alveolar wall. Cells "e" of I. persulcatus in their structure and functional activity closely resemble the earlier described cells "f" in alveoli III of the tick Rhipicephalus appendiculatus. 相似文献
9.
T lymphocytes of young and aged rats. II. Functional defects and the role of interleukin-2 总被引:2,自引:0,他引:2
S C Gilman J S Rosenberg J D Feldman 《Journal of immunology (Baltimore, Md. : 1950)》1982,128(2):644-650
Splenic T lymphocytes of aged Lewis rats respond to Con A and PHA with diminished 3H-TdR uptake compared with splenic T lymphocytes of young Lewis rats. After immunization with allogeneic tumor cells, uptake of 3H-TdR in mixed lymphocyte-tumor cultures and T cell cytotoxicity against tumor target cells are significantly lower with spleen cells of aged rats compared with those of young rats. The culture of spleen cells of aged rats with Con A results in a diminished conversion of Ia-positive T cells from Ia-negative precursors compared with similar cultures of spleen cells of young rats. Spleen cells of both young and aged rats produce high amounts of IL-2 in response to Con A stimulation. "Old" T cells, however, bind relatively little IL-2, do not utilize it in culture, and do not respond to exogenous IL-2 with enhanced 3H-TdR uptake as do "young" T cells. In allogeneic MLTC, "old" T lymphocytes produce little IL-2 compared with "young" cells, and both "young" and "old" cells respond to exogenous IL-2 with enhanced 3H-TdR uptake and increased cytotoxic activity. The data suggest defects in the synthesis and/or recognition of IL-2 as well as defects in the regulation of Ia antigen expression may be responsible, in part, for the reduced T cell function in aged animals. 相似文献
10.
Summary Measurable amounts of the nervous-system specific S-100 protein were detected by microcomplement fixation assay both in the superior cervical ganglion and in the adrenal medulla of adult rats, though at a significantly higher concentration in the ganglion. By the unlabeled antibody PAP method, the antigen was localized at: he ultrastructural level in the Schwann cells and in the satellite cells of the ganglion, but not in neurons. Similarly, the protein was found in the Schwann cells of the adrenal medulla, but not in the chromaffin cells. Moreover, the S-100 immunolabeling allowed detection of a class of satellite cells closely enveloping the chromaffin cells. In the labeled cells of both organs the reaction product was diffusely distributed in the cytoplasmic matrix as well as in the nucleoplasm.The presence of the S-100 antigen in the satellite cells of the sympathetic ganglion and in satellite cells of the adrenal medulla suggests a possible homology for the two cell types, and one could hypothesize the presence in peptide hormone-secreting endocrine organs of glia-like cells exhibiting functional relationships with the secretory cells comparable to those of the glial cells with the neurons. 相似文献
11.
The endocrine cells of the pyloric glands of adult ox 总被引:1,自引:0,他引:1
As part of a project to identify the endocrine cells ("EC" and "APUD" series) of the gastroenteric apparatus of ruminants, the ultrastructure of the mucosa of the pyloric glands of adult ox was studied morphologically and cytochemically, in parallel with a light microscope histochemical analysis. The results show that: the "EC" cells (producing 5-HT) are recognizable by their secretory granules which are heavily osmiophilic, argentaffin ("Masson") and argyrophilic ("Grimelius"). A further distinction is possible on the basis of their morphological features: the "EC" cells of the gastric type (which belong to the "ECn" group) contain granules fairly homogeneous in shape and size, while the "EC" cells of the intestinal type (or "EC1") show granules which are more pleiomorphic and variable in size. Of particular interest is the presence in some cells of granules typical of the "EC" cells of the intestinal type, in the vicinity of a few others, which appear quite similar to those of the adjoining exocrine cells; the "G" cells (gastrin producing) contain medium sized granules, which are unreactive to "Masson" and poorly argyrophilic. Their morphology is rather diverse; some of them (these are the "typical" cells) have a granular and weakly electron dense content, others (which we consider "atypical") show a homogeneous and heavily osmiophilic core, with an eccentrical empty area. Also present are granules whose appearance is intermediate and empty vesicles; the "D cells" (somatostatin producting) show round, medium sized granules which have a granular, moderately osmiophilic core, tightly encircled by the membrane. These granules are unreactive to "Masson" and to "Grimelius"; the "D1" cells (whose function is yet unclear) contain small, round granules whose core is variously but discretely electron dense and not always homogeneous; they are unreactive to "Masson" and fairly argyrophilic. These granules may be numerous and packed, or scarce; in this latter instance the few granules are intermingled with variously running tufts of parallel filaments, thus resembling the "P" cells, whose function is still undefined. These data show therefore that the types of endocrine cells we have identified in the pyloric glands of adult ox correspond to those described in other mammals; "X" and "F" or "PP" cell appear to be lacking. 相似文献
12.
Calabro C Albanese MP Martella S Licata P Lauriano ER Bertuccio C Licata A 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》2005,43(3):151-156
Glycosaminoglycans and NO synthase probably regulate mucous cell secretion in the skin of Tapes philippinarum. We have demonstrated the presence of "protein" cells, "glycogen" cells, "phenol" cells and five types of mucous cells, with different chemical composition of the mucus in the mantle epithelium of T. philippinarum. The foot epithelium contained "protein" cells and two types of mucous cells. Using biotinylated lectins, in the mantle and foot epithelia we have shown specific sites for the following oligosaccharides: alpha-D-glucose, alpha-D-mannose, alpha-L-fucose, alpha-D-1,3-N-acetyl-galactosamine and alpha-N-acetyl-glucosamine. nNOS immunoreactivity in the intraepithelial and intradermal cells and in the mucocytes suggested a regulatory role of NO in mucus secretion, as demonstrated also in other invertebrates. 相似文献
13.
Summary We have studied the effects of cocultivation on the frequency of mitomycin C (MMC)-induced chromosomal aberrations. This was carried out by cocultivating Fanconi anemia (FA) cells from the genetic complementation groups A and B with both normal mouse lymphoma L5178Y cells and the derived FA-like mutant cells, MCN-151 and MCE-50, assigned to complementation groups I and II, respectively. The results show a partial complementation of the defect (i.e. a reduction in the frequency of chromosomal aberration) in FA group A cells cocultured with normal or group II mouse cells, and a partial correction of mouse group I cells cocultived with normal or FA group B human cells. No reciprocal effects were observed between FA group A cells and mouse group I mutant cells; the frequencies of MMC-induced chromosomal aberrations in these cells remained unchanged by cocultivation. Moreover, no complementation was observed for both FA group B cells and mouse group II cells, after cocultivation with normal cells of either mouse or human origin. This implies that a diffusible factor released by normal human and mouse cells, and by FA group B and mouse group II mutant cells, can correct at least in part the chromosomal defect of FA group A and mouse group I mutant cells. With normal human or mouse cells, the frequency of chromosomal breakage after cocultivation remains the same as that observed in non-cocultived cells. This suggests that no detectable clastogenic factor is released by human FA or FA-like mouse cells. 相似文献
14.
Morphological studies on neuroglia 总被引:3,自引:0,他引:3
The postnatal development of microglial cells was investigated in the neonatal rat brain by use of light- and electron microscopy, including enzyme-histochemical techniques. Microglial cells were selectively stained by demonstration of their nucleoside diphosphatase (NDPase) activity and classified into three types: 1) In the early postnatal period "primitive microglial cells" showing scantily ramified processes were found in the cerebral cortex, the hippocampal formation, and the hypothalamus. During the course of the first postnatal week the processes of this cell type developed gradually and the cells were transformed into typical ramified microglial cells, called "resting microglial cells". 2) "Amoeboid microglial cells "showing typical features of macrophages were characteristic of the cerebral white matter. 3) "Round microglial cells" possessing a round soma and few pseudopodia but no characteristic processes occurred in large numbers in the subventricular zone of the lateral ventricle and as single elements in the vicinity of blood vessels. Histochemically, thiamine pyrophosphatase (TPPase) was demonstrated only in the fully developed, ramified microglial cells ("resting microglial cells"), which could be readily observed in the central nervous tissue from the age of 14 day. "Round and amoeboid microglial cells" did not show TPPase activity and disappeared after 14 days of postnatal life. By use of electron microscopy, in neonatal rats NDPase activity was apparent in the plasma membrane of the three types of microglial cells ("primitive, round, and amoeboid" types). They showed basically similar submicroscopic characteristics, i.e., well-developed Golgi apparatus, long strands of rough-surfaced endoplasmic reticulum, single dense bodies and vacuoles, and numerous ribosomes. "Amoeboid microglial cells" were characterized by their well-developed cytoplasmic vacuoles and phagocytic inclusion bodies. The present study strongly suggests a mesodermal origin for these microglial elements. 相似文献
15.
16.
Summary
Cephalosporium acremonium cells were immobilized in calcium alginate beads. Immobilized cells were used to produce -lactam antibiotics in rest medium under various oxygen concentrations, and the results were compared with free cell performance. Cell growth rate of immobilized cells was 35% of the growth rate of free cells. -Lactam antibiotic production rate of immobilized cells was also limited by mass transfer of oxygen. -Lactam antibiotic production rate of immobilized cells was 70% of that of free cells at oxygen saturation condition (i.e., 0.27 mM O2). Specific antibiotic production of immobilized cells was about 200% of that of free cells at 0.27 mM O2. 相似文献
17.
Regulatory T cells and tumor immunity 总被引:9,自引:0,他引:9
Chattopadhyay S Chakraborty NG Mukherji B 《Cancer immunology, immunotherapy : CII》2005,54(12):1153-1161
Central deletion of self-reactive T cells has been the textbook paradigm for inducing self-tolerance in the periphery and the concept of a role of T cell-mediated suppression in this process has long been controversial. A decisive shift in the opinion on suppressor T cells has lately occurred with the observations of Sakaguchis group that linked a class of CD4+CD25+ T cells to the prevention of autoimmunity from neonatal thymectomy in mice. These CD4+CD25+ T cells have been named T regulatory (Treg) cells. They are believed to be selected in the thymus as an anti-self repertoire. Hence they were referred to as natural T regulatory (nTreg) cells. Presently, in addition to their role in autoimmunity, they are believed to exert regulatory function in infection, in transplantation immunity as well as in tumor immunity. In contrast to these nTreg cells, another class of CD4+ Treg cells also exercises regulatory function in the periphery. These Treg cells are also CD4+ T cells and after activation they also become phenotypically CD4+CD25+. They are, however induced in the periphery as Treg cells. Hence, they are termed as induced Treg (iTreg) cells. There are major differences in the biology of these two types of Treg cells. They differ in their requirements for activation and in their mode of action. Nonetheless, evidence indicates that both nTreg cells and iTreg cells are involved in the control of tumor immunity. The question of how to circumvent their regulatory constraints, therefore, has become a major challenge for tumor immunologists. 相似文献
18.
Stanley A. Vinores Mary M. Herman Lucien J. Rubinstein 《The Histochemical journal》1987,19(8):439-448
Summary Neuron-specific () enolase, a glycolytic enzyme used as a relatively specific marker for normal neurons and neuroendocrine cells, has recently been found in a variety of neoplastic cells and in reactive astrocytes. Its localization was investigated by immunohisto- and electron-immunocyto-chemistry, in the proliferating supportive Schwann cells of a peripheral ganglioneuroblastoma and in the neoplastic Schwann cells of four acoustic tumours. By light microscopy, the neoplastic Schwann cells showed moderate uneven diffuse immunopositivity for enolase. By electron-immunocy-tochemistry, both types of Schwann cells demonstrated immunopositivity discretely limited to their cell surface membranes. The neoplastic ganglion cells and axons of the ganglioneuroblastoma and the normal neurons and axons included in the schwannomas were, as expected, intensely immunopositive. The visualization of enolase on the cell surface membranes of both neoplastic and non-neoplastic proliferating Schwann cells suggests that increased glycolytic activity may occur on the surface of these proliferating cells irrespective of the nature of the proliferation. 相似文献
19.
Zelivianski S Dean J Madhavan D Lin FF Lin MF 《Molecular and cellular biochemistry》2000,208(1-2):11-18
Receptor protein tyrosine phosphatase (RPTP) is transmembrane protein phosphatases, and has been proposed to be involved in the differentiation of the neuronal system. In the present study, we demonstrated the expression of RPTP mRNA in several normal human tissues. We further investigated the regulation of expression of RPTP mRNA in epithelial cells utilizing three commercially available human prostate cancer cell lines LNCaP, PC-3 and DU145. This is because these cells exhibit different levels of differentiation, defined by the expression of a tissue-specific differentiation antigen, prostatic acid phosphatase (PAcP), and their androgen sensitivity. LNCaP cells express PAcP and are androgen-sensitive cells, while PC-3 and DU145 cells do not express PAcP and are androgen-insensitive cells. Northern blot analyses revealed that, in LNCaP cells, fetal bovine serum (FBS) and 5-dihydrotestosterone (DHT) down-regulates RPTP mRNA expression, similar to the effect on PAcP. Contrarily, FBS up-regulated the RPTP mRNA level in PC-3 and DU145 cells. In LNCaP cells, sodium butyrate inhibited cell growth and up-regulated RPTP as well as PAcP mRNA expression. Although, sodium butyrate also inhibited the growth of PC-3 and DU145 cells, the level of RPTP mRNA was decreased in PC-3, while increased in DU145 cells. Thus, data taken together indicate that the expression of RPTP is apparently regulated by a similar mechanism to that of PAcP in LNCaP cells. 相似文献
20.
B. Sporrong P. Kannisto Prof. Ch. Owman N. -O. Sjöberg B. Walles 《Cell and tissue research》1985,241(3):505-511
Summary With the use of an anti-human S-100 protein antibody, it was possible to reveal a characteristic cell type in the anterior lobe of the normal human pituitary. These cells, so-called folliculo-stellate cells, were present in all pituitaries studied but their number varied from one gland to another. Immunoreactive cells, isolated or grouped, were arranged close to various secretory granulated cells. Especially by use of double immunoenzymatic labeling, it was evident that these cells are spatially related either to somatotropes, prolactin cells and corticotropes, or to glycoprotein-containing cells. Such immunoreactive cells were rare or absent in pseudo-follicular arrangements of secretory granulated cells. Since it is now possible to identify this cell type by light microscopy and since no reliable functional significance is known, it seems more advisable to term this cell type stellate cell instead of folliculostellate cell. 相似文献