Arabidopsis ARGONAUTE 1 Binds Chromatin to Promote Gene Transcription in Response to Hormones and Stresses

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Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance

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汉坦病毒S基因的克隆及体外转录

目的:通过基因克隆和体外转录,获得汉坦病毒汉滩型76118株及汉城型R22株S基因的RNA全长cRNA,为汉坦病毒病原学检测提供阳性定量标准品。方法:设计汉滩型76118株和汉城型R22株S基因克隆引物,PCR获得相应片段,分别克隆至含双启动子的PCRⅡ载体中,测序鉴定无误后,重组质粒分别经内切酶SpeⅠ、SacⅠ线性化,用T7 RNA聚合酶进行体外转录,产物经DNase处理、纯化后测定浓度,经RT-PCR验证。结果:获得汉滩型76118株及汉城型R22株S基因的cRNA片段,并可准确定量其拷贝数,76118株和R22株的质量浓度分别为80、17.58 ng/μL。结论:获得的cRNA样品可作为汉坦病毒核酸快速检测方法的阳性定量标准品。     

The Role of GAMYB Transcription Factors in GA-Regulated Gene Expression

A gibberellin- and abscisic acid-regulated MYB, GAMYB, was first identified as an activator of GA-regulated genes in cereal aleurone. Here we review recent advances made in delineating the signaling events related to GAMYB expression and function in aleurone. In addition, there is a growing body of evidence that GAMYB plays an important role in other aspects of plant growth and development, including anther development, stem elongation, floral initiation and seed development.