共查询到20条相似文献,搜索用时 31 毫秒
1.
Monica R. Canelhas Anne C. Barbosa Adriana O. Medeiros Ching-Fu Lee Li-Yin Huang Marc-André Lachance Carlos A. Rosa 《Antonie van Leeuwenhoek》2011,99(2):241-247
Two novel ascomycetous yeast species, Saturnispora serradocipensis and Saturnispora gosingensis, were isolated from leaf detritus in a tropical stream of Southeastern Brazil and a mushroom collected in Taiwan, respectively.
Analysis of the D1/D2 domains of the large-subunit of the rRNA gene of these strains showed that these species are related
to Saturnispora hagleri, their closest relative. Saturnispora serradocipensis and S. gosingensis differed from S. hagleri, respectively, by seven nucleotide substitutions and two indels and three nucleotide substitutions and three indels in D1/D2
rRNA sequences. The two new species differ from each another by four nucleotide substitutions and one indel in D1/D2 rRNA
sequences. However, the ITS sequences of S. serradocipensis, S. gosingensis and S. hagleri were quite divergent, showing that they are genetically separate species. The type strain of S. serradocipensis is UFMG-DC-198T (=CBS 11756T = NRRL Y-48717T), and of S. gosingensis GA4M05T is (CBS 11755T = NRRL Y-48718T). 相似文献
2.
Ching-Fu Lee Cheng-Hsu Yao Yi-Ru Liu Shuh-Sen Young Ku-Shang Chang 《Antonie van Leeuwenhoek》2009,95(4):335-341
A new yeast species, Kazachstania wufongensis, is proposed in this paper based on six strains isolated from soil in Taiwan. The species may produce one to four ellipsoidal
ascospores in each ascus, directly transformed from diploid cells. Genus assignment and distinction of the species from other
recognized species of Kazachstania is based on morphological and physiological characteristics, and on phylogenetic analysis of nucleotide sequences of the
D1/D2 domains of the large subunit (LSU) rRNA gene. Sequence analysis of the D1/D2 domains of the LSU rRNA gene reveals that
K. wufongensis is a member of the Kazachstania exigua complex, and its phylogenetically closest relatives are K. exigua, K. barnettii, K. bulderi, and K. turicensis. The species can be further differentiated from the other phylogenetically related species based on internal transcribed
spacer sequence and electrophoretic karyotype. Therefore, the new species Kazachstania wufongensis sp. nov. is proposed. The type strain of this new species, which was isolated from forest soil in Wufong, Hsinchu, Taiwan,
is FN21S03T (=CBS 10886T = BCRC 23138T). 相似文献
3.
Sreenivas Rao Ravella Stephen A. James Christopher J. Bond Ian N. Roberts Kathryn Cross Andy Retter Phil J. Hobbs 《Current microbiology》2010,60(1):12-16
Five yeast morphotypes were isolated from biogas reactors at North Wyke Research, Okehampton, UK. Out of the five morphotypes,
four were identified as known species. In contrast, the fifth morphotype strain, Bio10T, was found to differ from Bullera dendrophila and Kwoniella mangroviensis, its closest phylogenetic neighbours, by 2.6–2.9% with respect to the nucleotide sequence of the D1/D2 domain of the 26S
rRNA gene and by 5.6–6.2% with respect to the internal transcribed spacer 1 (ITS1)-5.8S rRNA gene-ITS2 region. Bio10T also differs from these two species by a number of phenotypic characteristics. Thus, based on the phenotypic differences
and phylogenetic analysis, strain Bio10T is assigned the status of a new species of Cryptococcus, for which the name Cryptococcus
shivajii sp. nov. is proposed. The type strain is Bio10T (NCYC 3541T = CBS 11374T). 相似文献
4.
Koowadjanakul N Jindamorakot S Yongmanitchai W Limtong S 《Antonie van Leeuwenhoek》2011,100(2):207-217
Five strains (LN12, LN14T, LN15T, LN16 and LN17T) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive
enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis
of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to
be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14T and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14T (= BCC 42666T = NBRC 107780T = CBS 12095T). Strain LN15T was assigned to be Candida chumphonensis sp. nov. (type strain LN15T = BCC 42667T = NBRC 107781T = CBS 12096T). Strain LN17T represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17T = BCC 42668T = NBRC 107782T = CBS 12097T). 相似文献
5.
Péter G Dlauchy D Tornai-Lehoczki J Gouliamova D Kurtzman CP 《Antonie van Leeuwenhoek》2011,100(3):375-383
Four ascosporulating strains of an undescribed methanol-assimilating yeast species were isolated from forest habitats in Hungary.
Three were recovered from rotten wood and one from leaves of a sessile oak (Quercus petraea). An additional isolate of the undescribed species sharing similar phenotypic characters with the above-noted strains was
recovered from the gut of an unidentified beetle collected from under the bark of a coniferous tree in Bulgaria. A closely
related, but somewhat divergent strain was recovered from insect frass in a Ponderosa pine (Pinus ponderosa) collected in New Mexico, USA. Analysis of the D1/D2 sequences of the LSU rRNA gene placed the new species in the Ogataea clade. The ITS and the D1/D2 LSU sequences of the rRNA gene repeats were compared for the above-noted strains and that of
the type strain of Ogataea zsoltii, the closest neighbour among currently recognized Ogataea species. Their relatedness was investigated by parsimony network analysis as well. As a result of the sequence analysis,
it was concluded that the six strains isolated from tree associated habitats represent a single new yeast species. Ogataea saltuana sp. nov. is proposed to accommodate these strains. The type strain NCAIM Y.01833T (CBS 10795T, NRRL Y-48448T) was recovered from rotten wood of Scotch pine (Pinus silvestris) in Hungary. The GenBank accession number for the D1/D2 domain nuclear large subunit rRNA gene sequence of strain NCAIM Y.01833T (CBS 10795T, NRRL Y-48448T) is EU327033. The MycoBank number of the new species is MB 519966. 相似文献
6.
Limtong S Koowadjanakul N Jindamorakot S Yongmanitchai W Nakase T 《Antonie van Leeuwenhoek》2012,102(2):221-229
Three strains (LM008T, LM068 and LM078T), representing two novel yeast species were isolated from the phylloplane of three plant species by an enrichment technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal spacer region, the three strains were assigned as two novel Candida species. Strain LM008T was assigned to be Candida sirachaensis sp. nov. (type strain LM008T = BCC 47628T = NBRC 108605T CBS 12094T) in the Starmerella clade. Two strains (LM068 and LM078T) represent a single species in the Lodderomyces-Spathaspora clade for which the name Candida sakaeoensis sp. nov. is proposed with the type strain LM078T = BCC 47632T = NBRC 108895T = CBS 12318T. 相似文献
7.
Chien-Hsun Huang Fwu-Ling Lee Chih-Jen Tien Pei-Wen Hsieh 《Antonie van Leeuwenhoek》2011,99(2):297-302
A novel anamorphic yeast strain, A1-01T, belonging to the genus Rhodotorula was isolated from a plant in Taiwan and analysed morphologically, physiologically and phylogenetically. Neither ballistoconidia
nor sexual reproduction was observed. Sequence analysis of the 26S rRNA gene and the ITS region indicate that Rhodosporidium sphaerocarpum is the most closely related species, with 14 and 24 nucleotide substitutions, respectively. The novel species differed physiologically
from R. sphaerocarpum in its ability to assimilate ethylamine and cadaverine, its inability to assimilate ethanol and nitrite. From these comparative
analyses, the following novel yeast species is proposed: Rhodotorula taiwanensis sp. nov. with the type strain of A1-01T (BCRC 23118T = CBS 11729T). 相似文献
8.
Four gram-negative, aerobic, motile, non-spore, forming rods with a wide pH and temperature range for growth (pH 7.0–11.0,
optimum pH 8.0; 20–45°C, optimum 28°C) strains were isolated from root nodules of Sphaerophysa salsula and characterized by means of a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that
the four strains formed a new lineage related to the genus Rhizobium and the sequence similarities between the isolate and the most related type strain Rhizobium giardinii was 96.5%. These strains also formed a distinctive group from the reference strains for defined Rhizobium species based on housekeeping gene sequences (atpD and recA), BOX-PCR fingerprinting, phenotypic features and symbiotic properties. The representative strain CCNWGS0238T has DNA-DNA relatedness of less than 33.4% with the most closely related species R. giardinii. It is therefore proposed as a new species, Rhizobium sphaerophysae sp. nov., with isolate CCNWGS0238T (=ACCC17498T = HAMBI3074T) as the type strain. 相似文献
9.
10.
Ravella SR Donovan N James SA Shivaji S Arunasri K Bond CJ Roberts IN Hobbs PJ 《Current microbiology》2011,63(2):115-120
Two yeast morphotypes, BET 4T and BET 7, were isolated from the gut of click beetle Melanotus villosus. Click beetles were collected from the decaying timber within the woodlands of North Wyke Research, South West England, UK
(latitude, 50°46′29″N; longitude, 3°55′23″W). Morphotype BET 7 was identified as Debaryomyces hansenii, and the other morphotype, BET 4T, was found to differs from Priceomyces castillae and Priceomyces haplophilus, its closest phylogenetic neighbours, by 5.0% with respect to the nucleotide sequence of the D1/D2 domain of the large-subunit
(LSU) rRNA gene, and by 8.0% with respect to the ribosomal internal-transcribed spacer (ITS) region. BET 4T also differ from P. castillae and P. haplophilus in a number of different phenotypic characteristics. Thus, based on the unique nucleotide sequences of its D1/D2 domain and
ITS region, its physiological characteristics and an inability to sporulate, strain BET 4T is assigned the status of a new species of Candida, for which the name Candida northwykensis sp. nov., is proposed. The type strain is BET 4T (NCYC 3525T = CBS 11370T). 相似文献
11.
In a taxonomic study of yeasts isolated from decaying plant materials submerged in water of mangrove forests in Thailand,
three strains isolated from tree bark (EM33T), a fallen leaf (EM40T) and a detached branch (SM56T) were found to represent three novel yeast species. On the basis of morphological, biochemical, physiological and chemotaxonomic
characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, and the phylogenetic analysis,
the three strains were assigned as three novel Candida species. They were named as Candida chanthaburiensis sp. nov. (type strain EM33T = BCC 23057T = NBRC 102176T = CBS 10926T), Candida kungkrabaensis sp. nov. (type strain EM40T = BCC 23060T = NBRC 102179T = CBS 10927T), and Candida suratensis sp. nov. (type strain SM56T = BCC 25961T = NBRC 103858T = CBS 10928T). 相似文献
12.
A strain named A18 was recovered from a compost of button mushrooms. It was characterized using a polyphasic approach. On
the basis of 16S rRNA gene sequence comparison, it belonged to the genus Nocardiopsis and was most closely related to the type strains of Nocardiopsis flavescens (sequence similarity 98.0%), Nocardiopsis prasina (97.5%), Nocardiopsis metallicus (97.4%), Nocardiopsis alba (97.3%). The combination of phylogenetic analysis, DNA–DNA hybridization, phenotypic characteristics and chemotaxonomic data
supported the proposal that strain A18 represents a new species of the genus Nocardiopsis, for which the name Nocardiopsis yanglingensis sp. nov. was proposed (type strain A18T = KCTC 19723T = CCTCC 209063T). 相似文献
13.
Roh SW Kim KH Nam YD Chang HW Kim MS Shin KS Yoon JH Oh HM Bae JW 《Journal of microbiology (Seoul, Korea)》2008,46(5):594-598
A novel Gram-negative and rod-shaped bacterium, designated N8T, was isolated from tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences showed that N8T strain is associated with the family Phyllobacteriaceae: two uncultured clones (98.4 and 99.8% 16S rRNA gene sequence similarity) and the genus Mesorhizobium (≤97.0%). The novel strain formed a separate clade with uncultured clones in the phylogenetic tree based on 16S rRNA gene
sequences. Cellular fatty acid profiles predominately comprised C18:1
ω7c and C19:0 cyclo ω8c. The major isoprenoid quinone is ubiquinone-10 and genomic DNA G+C content is 53.4 mol%. The polyphasic taxonomic study indicates
that the novel strain N8T represents a novel species of the new genus in the family Phyllobacteriaceae, named Aliihoeflea aestuarii. The type strain is N8T (= KCTC 22052T= JCM 15118T= DSM 19536T). 相似文献
14.
A new ascomycetous yeast species, Candida neustonensis is proposed in this study based on four strains (SN92T, SN47, SJ22, SJ25) isolated from sea surface microlayer in Taiwan. These four yeast strains were morphologically, physiologically
and phylogenetically identical to each other. No sexual reproduction was observed on 5% malt extract agar, corn meal agar,
V8 agar, McClary’s acetate agar and potato-dextrose agar. Phylogenetic analysis of the sequences of the D1/D2 domain of the
large subunit (LSU) rRNA gene places C. neustonensis as a member of the Pichia guilliermondii clade, it also reveals that the phylogenetically closest relatives of C. neustonensis are C. fukuyamaensis (4.4% divergence), C. xestobii (4.4% divergence) and P. guilliermondii (4.5% divergence). C. neustonensis also is clearly distinguished from other known species in the P. guilliermondii clade based on the results of physiology tests. From these comparison analyses, the following novel yeast species is proposed:
Candida neustonensis sp. nov., with strain SN92T (= BCRC 23108T = JCM 14892T = CBS 11061T) as the type strain. 相似文献
15.
Byung-Chun Kim Kang Hyun Lee Mi Na Kim Min Young Jung Young-Hyo Chang Jongtae Lee Kee-Sun Shin 《Current microbiology》2011,62(2):606-611
Strain M1-2T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic
taxonomic approach. Strain M1-2T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from
4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5T). The 16S rRNA gene sequence similarity level between M1-2T and J. marina En5T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2T contains MK-6 as the major menaquinone and iso-C15:0, summed feature 3 (C16:1 ω7c and/or iso-C15:0 2OH) and iso-C17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2T (= KCTC 23194T = NCAIM B.002413T). 相似文献
16.
Limtong S Jindamorakot S Am-In S Kaewwichian R Nitiyon S Yongmanitchai W Nakase T 《Antonie van Leeuwenhoek》2011,99(4):865-871
17.
The taxonomic study of two Gram-negative, aerobic, non-pigmented bacteria KMM 9010T and KMM 9023T isolated from a sandy sediment sample collected from the Sea of Japan seashore was performed. On the basis of the nearly
complete 16S rRNA gene sequences, strains KMM 9010T and KMM 9023T clustered with the Roseobacter lineage (class Alphaproteobacteria) forming a distinct phylogenetic line adjacent to the genus Donghicola. Novel strains shared the highest sequence similarity of 96.4% to each other and lower than 96.1% similarities to other validly
named genera of the class Alphaproteobacteria. In both strains, ubiquinone Q-10 was found to be the major respiratory quinone; phosphatidylcholine, phosphatidylglycerol,
diphosphatidylglycerol, phosphatidic acid, and an unknown aminolipid were the major polar lipids and C18:1ω7c and 11-methyl C18:1ω7c were predominant fatty acids. The DNA G+C content was 60.5 mol% (KMM 9010T) and 65.4 mol% (KMM 9023T). Based on phenotypic properties and phylogenetic evidence, strains KMM 9010T and KMM 9023T should be classified as two novel species in a new genus, Poseidonocella gen. nov., with Poseidonocella pacifica sp. nov., the type species with the type strain KMM 9010T (= NRIC 0794T = JCM 17310T), and Poseidonocella sedimentorum sp. nov. as the second species with the type strain KMM 9023T (= NRIC 0796T = JCM 17311T). 相似文献
18.
An agarolytic bacterium, designated as strain M5cT, was isolated from sea sand in Jeju Island, Korea. This isolate was Gram-negative, positive for catalase and oxidase, rod
and motile by means of monotrichous flagella. Strain M5cT has translucent or dark ivory colonies, forms a dent on an agar plate under colonies, and grows in the presence of 1–12%
(w/v) NaCl and at 10–37°C. This isolate hydrolyzes agar, alginic acid, carboxymethyl (CM)-cellulose and starch. Phylogenetic
analysis based on 16S rRNA gene sequences showed that strain M5cT can be considered as a species within the genus Gilvimarinus, being most closely related to Gilvimarinus chinensis QM42T, with a 16S rRNA gene sequence similarity of 95.6%. The major cellular fatty acids were C16:1ω7c and/or iso-C15:0 2OH (33.5%),
C16:0 (26.5%) and C18:1ω7c (14.1%). The DNA G+C content was 53.8 mol%. Based on these polyphasic data, strain M5cT should be classified as a novel species, for which the name Gilvimarinus agarilyticus sp. nov. is proposed. The type strain for the novel species is M5cT (= KCTC 23325T = NCAIM B 02425T). 相似文献
19.
A Gram-positive, rod-shaped, motile and spore-forming bacterium, designated ZLD-8T, was isolated from a desert soil sample collected from Xinjiang Province in north-west China, and subjected to a polyphasic
taxonomic analysis. This isolate grew optimally at 30°C and pH 7.0. It grew with 0–4% NaCl (optimum, 0–1%). Comparative 16S
rRNA gene sequence analysis showed that strain ZLD-8T was closely related to members of the genus Bacillus, exhibiting the highest 16S rRNA gene sequence similarity to Bacillus kribbensis DSM 17871T (98.0%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.3%. The DNA G + C content of strain ZLD-8T was 40.1 mol%. The strain contained MK-7 as the predominant menaquinone. The diagnostic diamino acid in the cell-wall peptidoglycan
was meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
The major fatty acids (>5% of total fatty acids) were anteiso-C15:0 (39.56%), iso-C14:0 (25.69%), C16:1 ω7c alcohol (10.13%) and iso-C15:0 (5.27%). These chemotaxonomic results supported the affiliation of strain ZLD-8T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation
of strain ZLD-8T from recognized Bacillus species. On the basis of the polyphasic evidence presented, strain ZLD-8T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus
deserti sp. nov. is proposed. The type strain is ZLD-8T (=CCTCC AB 207173T = KCTC 13246T). 相似文献
20.
Annarita Poli Ida Romano Paolo Cordella Pierangelo Orlando Barbara Nicolaus Cristina Ceschi Berrini 《Extremophiles : life under extreme conditions》2009,13(6):867-874
A novel aerobe thermophilic endospore-forming bacterium designated strain AF/04T was isolated from thermal mud located in Euganean hot springs, Abano Terme, Padova, Italy. Strain AF/04T was Gram-positive, motile, rod-shaped, occurring in pairs, or filamentous. The isolate grew between 55 and 67°C (optimum
65°C) and at pH 6.0–7.5 (optimum pH 7.2). The strain was aerobic and grew on maltose, trehalose, and sodium acetate as sole
carbon sources. The G + C content of DNA was 53.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that
strain AF/04T falls within the genus Anoxybacillus. Levels of 16S rRNA gene sequence similarity between strain AF/04T and the type strains of recognized Anoxybacillus species ranged from 95 to 99%. Chemotaxonomic data (major isoprenoid quinone–menaquinone-7; major fatty acid iso-C15:0 and anteiso-C17:0) supported the affiliation of strain AF/04T to the genus Anoxybacillus. Based on phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA hybridization data, it was proposed that strain AF/04T (=DSM 17141T = ATCC BAA 1156T) should be placed in the genus Anoxybacillus as the type strain of a novel species, Anoxybacillus thermarum sp. nov. 相似文献