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1.
Excitatory postsynaptic potentials (EPSP's) generated in crab muscle fibers by a single motor axon, differ in amplitude and facilitation. Some EPSP's are large at low frequencies of stimulation and show little facilitation; others are smaller and show pronounced facilitation. When K+ is replaced by Cs+ in the physiological solution, all EPSP's increase in amplitude, but small EPSP's increase proportionately more than large ones. Quantal content of transmission, determined by external recording at single synaptic regions, undergoes a much larger increase at facilitating synapses. The increase in quantal content of transmission is attributable to prolongation of the nerve terminal action potential in Cs+. After 1–2 h of Cs+ treatment, defacilitation of synaptic potentials occurs at synapses which initially showed facilitation. This indicates that Cs+ treatment drastically increases the fraction of the "immediately available" transmitter store released by each nerve impulse, especially at terminals with facilitating synapses. It is proposed that facilitating synapses normally release less of the "immediately available" store of transmitter than poorly facilitating synapses. Possible reasons for this difference in performance are discussed. 相似文献
2.
P. Valli G. Zucca L. Botta C. Casella 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1984,155(6):739-743
Summary The specificity of the K-channels which are present in the ciliated membrane of ampullar receptors has been investigated by replacing K+ in the fluids bathing the canal with Rb+ and Cs+. Results show that, unlike Cs+, Rb+ is able to substitute K+ in maintaining the receptor function. These findings favour the hypothesis that the transduction channels which allow the receptor current to flow across sensory cell bodies are specific K-channels. The effects of Rb+ and Cs+ on primary sensory neuron endings were also studied.Abbreviations
Adc
slow ampullar potentials
-
Ndc
slow nerve potentials 相似文献
3.
Pharmacological and gene regulation properties point to the SlHAK5 K+ transporter as a system for high‐affinity Cs+ uptake in tomato plants
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Manuel Nieves‐Cordones Rosa M. Rivero Vicente Martinez Francisco Rubio 《Physiologia plantarum》2018,162(4):455-466
Potassium (K+) and cesium (Cs+) are chemically similar but while K+ is an essential nutrient, Cs+ can be toxic for living organisms, plants included. Two different situations could lead to problems derived from the presence of Cs+ in agricultural systems: (1) presence of Cs+ at high concentrations that could produce toxic effects on plants, (2) presence of micromolar concentrations of radiocesium, which can be accumulated in the plant and affect animal and human health through the food chain. While K+ uptake has been well described in tomato plants, information on molecular mechanisms involved in Cs+ accumulation in this species is absent. Here, we show that in tomato plants, high concentrations of Cs+ produce deficiency of K+ but do not induce high‐affinity K+ uptake or the gene encoding the high‐affinity K+ transporter SlHAK5. At these concentrations, Cs+ uptake takes place through a Ca2+‐sensitive pathway, probably a non‐selective cation channel. At micromolar concentrations, Cs+ is accumulated by a high‐affinity uptake system upregulated in K+‐starved plants. This high‐affinity Cs+ uptake shares features with high‐affinity K+ uptake. It is sensitive to NH4+ and insensitive to Ba2+ and Ca2+ and its presence parallels the pattern of SlHAK5 expression. Moreover, blockers of reactive oxygen species and ethylene action repress SlHAK5 and negatively regulate both high‐affinity K+ and Cs+ uptake. Thus, we propose that SlHAK5 contributes to Cs+ uptake from micromolar concentrations in tomato plants and can constitute a pathway for radiocesium transfer from contaminated areas to the food chain. 相似文献
4.
Caesium accumulation by microorganisms: uptake mechanisms,cation competition,compartmentalization and toxicity 总被引:4,自引:0,他引:4
Simon V. Avery 《Journal of industrial microbiology & biotechnology》1995,14(2):76-84
Summary The continued release of caesium radioisotopes into the environment has led to a resurgence of interest in microbe-Cs interactions. Caesium exists almost exclusively as the monovalent cation Cs+ in the natural environment. Although Cs+ is a weak Lewis acid that exhibits a low tendency to form complexes with ligands, its chemical similarity to the biologically essential alkali cation K+ facilitates high levels of metabolism-dependent intracellular accumulation. Microbial Cs+ (K+) uptake is generally mediated by monovalent cation transport systems located on the plasma membrane. These differe widely in specificity for alkali cations and consequently microorganisms display large differences in their ability to accumulate Cs+; Cs+ appears to have an equal or greater affinity than K+ for transport in certain microorganisms. Microbial Cs+ accumulation is markedly influenced by the presence of external cations, e.g. K+, Na+, NH4
+ and H+, and is generally accompanied by an approximate stoichiometric exchange for intracellular K+. However, stimulation of growth of K+-starved microbial cultures by Cs+ is limited and its has been proposed that it is not the presence of Cs+ in cells that is growth inhibitory but rather the resulting loss of K+. Increased microbial tolerance to Cs+ may result from sequestration of Cs+ in vacuoles or changes in the activity and/or specificity of transport systems mediating Cs+ uptake. The precise intracellular target(s) for Cs+-induced toxicity has yet to be clearly defined, although certain internal structures, e.g. ribosomes, become unstable in the presence of Cs+ and Cs+ is known to substitute poorly for K+ in the activation of many K+-requiring enzymes. 相似文献
5.
The mechanisms of the hyperpolarizing and depolarizing actions of cesium were studied in cardiac Purkinje fibers perfused in vitro by means of a microelectrode technique under conditions that modify either the Na+-K+ pump activity or If. Cs+ (2 mM) inconsistently increased and then decreased the maximum diastolic potential (MDP); and markedly decreased diastolic depolarization (DD). Increase and decrease in MDP persisted in fibers driven at fast rate (no diastolic interval and no activation of If). In quiescent fibers, Cs+ caused a transient hyperpolarization during which elicited action potentials were followed by a markedly decreased undershoot and a much reduced DD. In fibers depolarized at the plateau in zero [K+]o (no If), Cs+ induced a persistent hyperpolarization. In 2 mM [K+]o, Cs+ reduced the undershoot and suppressed spontaneous activity by hyperpolarizing and thus preventing the attainment of the threshold. In 7 mM [K+]o, DD and undershoot were smaller and Cs+ reduced them. In 7 and 10 mM [K+]o, Cs+ caused a small inconsistent hyperpolarization and a net depolarization in quiescent fibers; and decreased MDP in driven fibers. In the presence of strophanthidin, Cs+ hyperpolarized less. Increasing [Cs+]o to 4, 8 and 16 mM gradually hyperpolarized less, depolarized more and abolished the undershoot. We conclude that in Purkinje fibers Cs+ hyperpolarizes the membrane by stimulating the activity of the electrogenic Na+-K+ pump (and not by suppressing If); and blocks the pacemaker potential by blocking the undershoot, consistent with a Cs+ block of a potassium pacemaker current. 相似文献
6.
On the mechanism of cesium-induced voltage and current tails in single ventricular myocytes 总被引:1,自引:1,他引:0
The mechanisms by which different concentrations of cesium modify membrane potentials and currents were investigated in guinea pig single ventricular myocytes. In a dose-dependent manner, cesium reversibly decreases the resting potential and action potential amplitude and duration, and induces a diastolic decaying voltage tail (Vex), which increases at more negative and reverses at less negative potentials. In voltage-clamped myocytes, Cs+ increases the holding current, increases the outward current at plateau levels while decreasing it at potentials closer to resting potential, induces an inward tail current (Iex) on return to resting potential and causes a negative shift of the threshold for the inward current. During depolarizing ramps, Cs+ decreases the outward current negative to inward rectification range, whereas it increases the current past that range. During repolarizing ramps, Cs+ shifts the threshold for removal of inward rectification negative slope to less negative values. Cs+-induced voltage and current tails are increased by repetitive activity, caffeine (5 mM) and high [Ca2+]o (8.1 mM), and are reduced by low Ca2+ (0.45 mM), Cd2+ (0.2 mM) and Ni2+ (2 mM). Ni2+ also abolishes the tail current that follows steps more positive than ECa. We conclude that Cs+ (1) decreases the resting potential by decreasing the outward current at more negative potentials, (2) shortens the action potential by increasing the outward current at potentials positive to the negative slope of inward rectification, and (3) induces diastolic tails through a Ca2+-dependent mechanism, which apparently is an enhanced electrogenic Na-Ca exchange. 相似文献
7.
Production of low‐Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR‐Cas system
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Manuel Nieves‐Cordones Sonia Mohamed Keitaro Tanoi Natsuko I. Kobayashi Keiko Takagi Aurore Vernet Emmanuel Guiderdoni Christophe Périn Hervé Sentenac Anne‐Aliénor Véry 《The Plant journal : for cell and molecular biology》2017,92(1):43-56
The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm ), cesium (Cs+) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs+ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs+ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side‐effects. Conversely, the development of biotechnological approaches aimed at reducing Cs+ accumulation in rice remain challenging. Here, we show that inactivation of the Cs+‐permeable K+ transporter OsHAK1 with the CRISPR‐Cas system dramatically reduced Cs+ uptake by rice plants. Cs+ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs+ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs+ and K+; and (ii) a high capacity to transport Cs+ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with 137Cs+, plants lacking OsHAK1 function displayed strikingly reduced levels of 137Cs+ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents. 相似文献
8.
The influence of alkali ions on the circadian leaf movements of Oxalis regnellii Mig. was investigated. Ions were given to the oscillating system via the transpiration stream of cut stalks in nutrient medium. Chloride solutions of Rb+, Cs+, Na+ and K+ were tested and the results compared to previously published LiCl-results. The period of the circadian leaf movements was unaffected by a continual addition of Na+ or K+ to the nutrient medium (at least up to 40 mM). Rb+, in the concentration of 2.5 or 5 mM, caused a shortening of the period when applied continuously. Rb+ concentrations up to 60 mM were tested. Cs+ ions caused only lengthenings of the circadian period. Cs+ concentrations up to 40 mM were tested. Cs+ resembled Li+ in producing period lengthenings, but was not as effective as Li+ when compared on a concentration basis. Toxicity of the effective ions was in the following order: Li+Cs+Rb+, Rb+ pulses (50 mM, 4 h) phase-shifted the rhythm and caused advances. A phase response curve was determined and the maximum steady state advances were of the order of 1 h. The dual effect of the Rb+ ions is discussed and is assumed to be due to two counteracting processes, exemplified by Rb+-sensitive ATPase-controlled pumping processes and protein synthesis. For comparison, the effects of Rb+ and Li+ in human depressive disorders is also discussed in relation to their influence on circadian systems. It is emphasized that Rb+ and K+ behave differently and are not interchangeable in their action on circadian systems. 相似文献
9.
Isolated small intestine of toad (Bufo bufo) was mounted on glass tubes for perfusion studies with oxygenated amphibian Ringer's solution containing glucose and acetate.
Under open-circuit conditions (V
t
=−3.9 ± 1.8 mV, N= 14) the preparation generated a net influx of 134Cs+. The time course of unidirectional 134Cs+-fluxes was mono-exponential with similar rate constants for influx and outflux when measured in the same preparation. The
flux-ratio was time invariant from the beginning of appearance of the tracers to steady state was achieved. Thus, just a single
pathway, the paracellular pathway, is available for transepithelial transport of Cs+. From the ratio of unidirectional Cs+-fluxes the paracellular force was calculated to be, 18.2 ± 1.5 mV (N= 6), which is directed against the small transepithelial potential difference. The paracellular netflux of cesium ions, therefore,
is caused by solvent drag. The flux of 134Cs+ entering and trapped by the cells was of a magnitude similar to that passing the paracellular route. Therefore, independent
of the convective flux of 134Cs+, every second 134Cs+ ion flowing into the lateral space was pumped into the cells rather than proceeding, via the low resistance pathway, to the
serosal bath. It is thus indicated that the paracellular convective flow of 134Cs+ is driven by lateral Na+/K+-pumps. Transepithelial unidirectional 42K+ fluxes did not reach steady state within an observation period of 70 min, indicating that components of the fluxes in both
directions pass the large cellular pool of potassium ions. The ratio of unidirectional 24Na+ fluxes was time-variant and declined from an initial value of 3.66 ± 0.34 to a significantly smaller steady-state value of
2.57 ± 0.26 (P < 0.001, N= 5 paired observations), indicating that sodium ions pass the epithelium both via the paracellular and the cellular pathway.
Quantitatively, the larger ratio of paracellular Na+ fluxes, as compared to that of paracellular Cs+ fluxes, is compatible with convective flow of the two alkali metal ions through the same population of water-filled pores.
With a new set of equations, the fraction of the sodium flux passing the basement membrane barrier of the lateral space that
is recirculated through the cellular compartment is estimated. This fraction was, on average, 0.72 ± 0.03 (N= 5). It is concluded that isotonicity of the transportate can be maintained by producing a hypertonic fluid emerging from
the lateral space combined with reuptake of salt via the cells.
Received: 14 October 1998/Revised: 14 January 1999 相似文献
10.
K+ channel gating currents are usually measured in the absence of permeating ions, when a common feature of channel closing is a rising phase of off-gating current and slow subsequent decay. Current models of gating invoke a concerted rearrangement of subunits just before the open state to explain this very slow charge return from opening potentials. We have measured gating currents from the voltage-gated K+ channel, Kv1.5, highly overexpressed in human embryonic kidney cells. In the presence of permeating K+ or Cs+, we show, by comparison with data obtained in the absence of permeant ions, that there is a rapid return of charge after depolarizations. Measurement of off-gating currents on repolarization before and after K+ dialysis from cells allowed a comparison of off-gating current amplitudes and time course in the same cells. Parallel experiments utilizing the low permeability of Cs+ through Kv1.5 revealed similar rapid charge return during measurements of off-gating currents at ECs. Such effects could not be reproduced in a nonconducting mutant (W472F) of Kv1.5, in which, by definition, ion permeation was macroscopically absent. This preservation of a fast kinetic structure of off-gating currents on return from potentials at which channels open suggests an allosteric modulation by permeant cations. This may arise from a direct action on a slow step late in the activation pathway, or via a retardation in the rate of C-type inactivation. The activation energy barrier for K+ channel closing is reduced, which may be important during repetitive action potential spiking where ion channels characteristically undergo continuous cyclical activation and deactivation. 相似文献
11.
Manuel Nieves-Cordones Alberto Lara Martha Silva Jesús Amo Pascual Rodriguez-Sepulveda Rosa M. Rivero Vicente Martínez M. Angeles Botella Francisco Rubio 《Plant, cell & environment》2020,43(7):1707-1721
Root K+ acquisition is a key process for plant growth and development, extensively studied in the model plant Arabidopsis thaliana. Because important differences may exist among species, translational research supported by specific studies is needed in crops such as tomato. Here we present a reverse genetics study to demonstrate the role of the SlHAK5 K+ transporter in tomato K+ nutrition, Cs+ accumulation and its fertility. slhak5 KO lines, generated by CRISPR-Cas edition, were characterized in growth experiments, Rb+ and Cs+ uptake tests and root cells K+-induced plasma membrane depolarizations. Pollen viability and its K+ accumulation capacity were estimated by using the K+-sensitive dye Ion Potassium Green 4. SlHAK5 is the major system for high-affinity root K+ uptake required for plant growth at low K+, even in the presence of salinity. It also constitutes a pathway for Cs+ entry in tomato plants with a strong impact on fruit Cs+ accumulation. SlHAK5 also contributes to pollen K+ uptake and viability and its absence produces almost seedless fruits. Knowledge gained into SlHAK5 can serve as a model for other crops with fleshy fruits and it can help to generate tools to develop low Cs+ or seedless fruits crops. 相似文献
12.
Caesium uptake and toxicity in the cyanobacterium <Emphasis Type="Italic">Nostoc muscorum</Emphasis>
Santosh Bhargava 《World journal of microbiology & biotechnology》2007,23(2):305-307
A NH4+ transport-defective mutant and a K+ transport-defective mutant of the cyanobacterium Nostoc muscorum were analysed with regard to percentage survival as a function of CsCl toxicity and Cs+ uptake activity. Neither survival nor Cs+ uptake was affected in either of the two mutants when compared with the wild type. The results indicate that the toxicity
of Cs+ is determined at more than one cellular site in this organism. 相似文献
13.
L. Edelmann 《Histochemistry and cell biology》1980,67(3):233-242
Summary Normal frog sartorius muscles and muscles in which a major portion of the intracellular K+ was reversibly replaced by Rb+ or Cs+ were frozen, freeze-dried and embedded without chemical fixation or staining. Dry-cut sections of these preparations reveal striation patterns with higher contrast than those of wet-cut sections of the same preparation. The results suggest that in the living state the alkali metal ions are mainly localized in the A bands and Z lines of myofibrils. This idea is confirmed by a new autoradiographic technique by means of which the distribution of Rb+ and Cs+ in frozen-hydrated single muscle fibers has been investigated. The findings support the association-induction hypothesis according to which most cell K+ and other alkali-metal ions are not free in cell water but are adsorbed to beta- and gamma-carboxyl groups of cell proteins. 相似文献
14.
The association of two gramicidin A (gA) peptides via H-bonds in lipid bilayers causes the formation of an ion channel that
is selective for monovalent cations only. In this study, two gAs were covalently linked with a dioxolane group (SS dimer).
Some functional properties of natural gA channels were compared to that synthetic dimer in Na+- or Cs+-containing solutions. The SS dimer remained in the open configuration most of the time, while natural gA channels had a relatively
brief mean open time. Single channel conductances to Na+ (g
Na
) or Cs+ (g
Cs
) in the SS dimer were smaller than in natural gA. However, g
Na
was considerably more attenuated than g
Cs
. This probably results from a tight solvation of Na+ by the dioxolane linker in the SS channel. In Cs+ solutions, the SS had frequent closures. By contrast, in Na+ solutions the synthetic dimer remained essentially in the open state. The mean open times of SS channels in different solutions
(T
open,Na
> T
open,Cs
> T
open,H
) were inversely proportional to the single channel conductances (g
H
> g
Cs
> g
Na
). This suggests that ion occupancy inside the pore stabilizes the open configuration of the gA dimer. The mean closed time
of the SS dimer was longer in Cs+ than in H+ solutions. Possible mechanisms for these effects are discussed.
Received: 17 September 1999/Revised: 21 December 1999 相似文献
15.
Abhishek K. Gupta 《Molecular simulation》2017,43(8):625-637
Molecular dynamic simulations of anionic polyelectrolyte poly(acrylic acid) (PAA) in water–ethanol solution, specifically Li+-PAA and Cs+-PAA, were carried out across the solvent composition range 0 ≤ фeth ≤ 0.9. Chain collapse (i.e. shrinkage) occurs with increase in фeth for both types of counter-ion systems and in agreement with the experiments. The qualitative difference in the collapse point is in agreement with experimental results, with counter-ion specific chain collapse of PAA following the order Li+ > Cs+. With increase in фeth the number of hydrogen-bonds between PAA and water decreases while that between PAA and ethanol increases. At higher level of ethanol content in solution, ethanol molecules displace water molecules from the vicinity of the chain. The analysis of the radial distribution functions shows that counter-ion binding distance of Li+ to chain is lesser as compared to that of Cs+, as well as a higher coordination number exhibited by Li+. Thus, as compared to Cs+-PAA, greater number of contact ion pairs formed between Li+ and PAA induce chain collapse more easily. The coordination of Li+ to PAA is better than that of Cs+ throughout the фeth range, which could be the reason for the greater extent of PAA chain shrinkage observed in the case of Li+. Binding of water molecule to PAA units is stronger in the case of Cs+. The backbone dihedral trans probability of both systems displayed a decrease with фeth indicating chain shrinkage. The relaxation time of H-bonds between PAA and EtOH is greater for Li+-PAA as compared to Cs+-PAA system. The enhancement of counter-ion pairs formation is found to be directly responsible for the solvent composition at which chain collapse occurs in the particular system. 相似文献
16.
Metamorphosis of the planula larvae into polyps does not occur spontaneously but depends on the reception of external trigger stimuli. Artificially, metamorphosis can be initiated by a pulse-type application of Cs+ or tumor-promoting phorbol esters (W. A. Müller (1985) Differentiation 29, 216–222). In the present study we examined the putative involvement of the phosphatidylinositol system in signal transduction. Planulae of Hydractinia echinata were preincubated with [3H]-inositol. Upon exposure of the larvae to Cs+ the label in inositol trisphosphate (InsP3) increased twofold as early as 15 sec after addition of Cs+. Within the first 60 sec the levels of inositol monophosphate (InsP1) and inositol bisphosphate (InsP2) were also elevated compared to the values in nonstimulated larvae. After 1 and 3 hr, respectively, of incubation with Cs+, only the label in InsP2 was increased. When applied to saponin-permeabilized larvae, InsP3 did not induce metamorphosis. But 1,2-dioctanoyl-rac-glycerol (diC8) was effective in inducing metamorphosis with a half-maximal effective concentration of 9 μM. The percentage of metamorphosed animals after the application of 5 μM diC8 (30 mM Cs+) was increased by the simultaneous application of 1 μM (0.1 μM) of the diacylglycerol kinase inhibitor R 59022. The results are interpreted as evidence for the involvement of the PI-signaling/diacylglycerol transduction system in the initiation of metamorphosis of planula larvae of H. echinata. 相似文献
17.
Raúl Alva J. Antonio Lugo-R Eduardo Arzt Jorge Cerbón Blanca E. Rivera Mireya Toro Sergio Estrada-O 《Journal of bioenergetics and biomembranes》1992,24(1):125-129
Nigericin is a monocarboxylic polyether molecule described as a mobile K+ ionophore unable to transport Li+ and Cs+ across natural or artificial membranes. This paper shows that the ion carrier molecule forms complexes of equivalent energy demands with Li+, Cs+, Na+, Rb+, and K+. This is in accordance with the similar values of the complex stability constants obtained from nigericin with the five alkali metal cations assayed. On the other hand, nigericinalkali metal cation binding isotherms show faster rates for Li+ and Cs+ than for Na+, K+, and Rb+, in conditions where the carboxylic proton does not dissociate. Furthermore, proton NMR spectra of nigericin-Li+ and nigericin-Cs+ complexes show wide broadenings, suggesting strong cation interaction with the ionophore; in contrast, the complexes with Na+, K+, and Rb+ show only clear-cut chemical shifts. These latter results support the view that nigericin forms highly stable complexes with Li+ and Cs+ and contribute to the explanation for the inability of this ionophore to transport the former cations in conditions where it catalyzes a fast transport of K+>Rb+>Na+.Part of the results of this paper were presented at the 14th International Congress of Biochemistry in Prague, Czechoslovakia. 相似文献
18.
Mark Tester 《The Journal of membrane biology》1988,105(1):87-94
Summary The outer membranes of plant cells contain channels which are highly selective for K+. In the giant-celled green algaChara corallina, K+ currents in the plasmalemma were measured when the cell was depolarized to the K+ equilibrium potential in relatively high external K+ concentrations. K+ current was reduced by externally added Cs+. Cs+ mainly inhibited inward K+ current, in a strongly voltage-dependent manner; the effective valence of the blocking reaction was often greater than 1, increasing with higher external Cs+ concentrations and with lower K+ concentrations. This is consistent with the channels being single-file, multi-ion pores. Outward current could also be inhibited by Cs+, when external K+ concentrations were low relative to Cs+ concentrations. As the ratio of K+/Tl+ was changed (keeping the sum of the two ions equal), both the resting potential and plasmalemma conductance went through minimums; this is the so-called anomalous mole fraction effect, and is consistent with a channel whose pore can be multiply occupied. These effects together strongly suggest that the K+ channels found in the plasmalemma ofChara are multi-ion pores. 相似文献
19.
Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons 总被引:44,自引:25,他引:19
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Internal Cs+, Na+, Li+, and, to a lesser degree, Rb+ interfere with outward current through the K pores in voltage clamped squid axons. Addition of 100 mM NaF to the perfusion medium cuts outward current for large depolarizations about in half, and causes negative conductance over a range of membrane voltages. For example, suddenly reducing membrane potential from +100 to +60 mv increases the magnitude of the outward current. Internal Cs+ and, to a small extent, Li+, also cause negative conductance. Na+ ions permeate at least 17 times less well through the K pores than K+, and Cs+ does not permeate measurably. The results strongly suggest that K pores have a wide and not very selective inner mouth, which accepts K+, Na+, Li+, Cs+, tetraethylammonium ion (TEA+), and other ions. The diameter of the mouth must be at least 8 A, which is the diameter of a TEA+ ion. K+ ions in the mouths probably have full hydration shells. The remainder of the pore is postulated to be 2.6–3.0 A in diameter, large enough for K+ and Rb+ but too small for Cs+ and TEA+. We postulate that Na+ ions do not enter the narrower part of the pore because they are too small to fit well in the coordination cages provided by the pore as replacements for the water molecules surrounding an ion. 相似文献
20.
The rod cells in frog taste discs display the outward current and maintain the negative resting potential in the condition where internal K+ is replaced with Cs+. We analyzed the properties of the Cs+-permeable conductance in the rod cells. The current–voltage (I/V) relationships obtained by a voltage ramp were bell-shaped under Cs+ internal solution. The steady state I/V relationships elicited by voltage steps also displayed the bell-shaped outward current. The activation of the current accelerated with the depolarization and the inactivation appeared at positive voltage. The gating for the current was maintained even at symmetric condition (Cs+ external and internal solutions). The wing cells did not show the properties. The permeability for K+ was a little larger than that for Cs+. Internal Na+ and NMDG+ could not induce the bell-shaped outward current. Carbenoxolone inhibited the bell-shaped outward Cs+ current dose dependently (IC50: 27 μM). Internal arachidonic acid (20 μM) did not induce the linear current–voltage (I–V) relationship which is observed in two-pore domain K+ channel (K2P). The results suggest that the resting membrane potentials in the rod cells are maintained by the voltage-gated K+ channels. 相似文献