"Axial split osteotomy" of free fibular flaps for mandible reconstruction: preliminary results

Vascularized free flaps are now considered the most appropriate choice for mandible reconstruction because they offer excellent cosmetic and functional results. Various techniques have been proposed. Free fibular flaps have numerous advantages in terms of versatility and contouring. Shaping can be achieved by wedge osteotomy with excellent results. However, this technique leads to bone loss and may be difficult in the later stages of the procedure. The purpose of this report is to describe a simple and safe flap-shaping method involving axial split osteotomy.     

一种用于昆虫性信息素成分单不饱和双键定位的简便方法

昆虫利用特定的性信息素成分实现种内信息交流和种间生殖隔离,性信息素成分异构体的细微差异都能导致其生物活性的很大改变。本研究利用二甲基二硫醚（DMDS）甲硫基化反应和气相色谱-电子轰击质谱（GC-EI-MS）的方法对脂肪族单不饱和醇、醛和乙酸酯类昆虫性信息素成分双键在碳链中的位置异构进行系统研究。质谱分析结果表明,含有醇和乙酸酯官能团的单不饱和烯烃甲硫基化衍生物的质谱特征碎片分子离子峰（M+）及2个主要的诊断离子m/z 61+14m ［H-（CH₂）_m-CH=S⁺ CH₃］和77+14n［CH₃S⁺ =CH（CH₂）_nOH］（或119+14n,［CH₃S⁺ =CH（CH₂）_nOOCCH₃］）丰度都很强,可以据此直接推断双键在碳链中的位置。尽管单不饱和醛类化合物DMDS衍生物的特征碎片峰也很明显,但是由于羰基的质量数和2个亚甲基的质量数相同,不能由低分辨质谱的特征碎片峰直接推断双键在碳链中的位置。DMDS甲硫基化反应结合GC-EI-MS分析方法鉴定昆虫性信息素成分单不饱和双键在碳链中的位置异构,该方法简单快捷,为昆虫性信息素的结构鉴定工作奠定了坚实的基础。     

A method for computing the three-dimensional angular velocity and acceleration of a body segment from three-dimensional position data

A theoretical technique, based on the Method of Least Squares, was employed to solve for the three-dimensional components of the angular velocity and acceleration of a limb segment directly from experimentally recorded three-dimensional position data. Results showed that a minimum of four targets placed on the body segment, forming six relative position vector equations, were required to produce the most accurate results. It was also found that this method eliminates the errors due to soft tissue motion and system noise.     

A simple method to change geometrical proportions of muscles by means of a tibial lengthening osteotomy in the rat

A method is designed to change geometrical proportions of muscles in the rat. After a tibial osteotomy, the application of a stainless steel prosthesis resulted in an elongation of about 8% of the tibia. In 23 out of 28 operated rats a correct application of the prosthesis was maintained till the rats were sacrificed. The operation caused hardly any muscle tissue damage. No disuse of the manipulated leg was observed in the operated rats.     

A simple method for the purification of myxobacteria

Pure cultures of many myxobacteria could be quickly obtained by treating fruiting bodies with highly dosed mixtures of antibiotics     

A simple method for prevention of coprophagy in the mouse

A series of studies of coprophagy in mice has been conducted from the viewpoints of behaviour and nutrition. Since it was necessary to prevent coprophagy to investigate its significance, an attempt was made to devise a restrainer. A polyethylene bottle was cut and fixed to the mouse so as to prevent its head from reaching its anus when faeces were excreted. The animals were also housed in cages with floors of larger mesh size in order to let the faeces fall through.     

A method for establishing cell lines fromDrosophila melanogaster embryos

Summary A simple method is presented for establishing continuous cell lines fromDrosophila melanogaster embryos. Subculturing is performed after the first 8 weeks and at 2-week intervals therafter. Initial plating densities of 5×10⁴ to 5×10⁵ cells per cm² are required for maintaining the subcultures. Cell lines were established from wild-type embryos, from embryos bearing chromosomal rearrangements and from embryos bearing recessive mutations. Permanent lines have doubling times of 24 to 48 hr and have been maintained for as long as 13 months and 25 subcultures. Supported in part by NSF grant BMS75-02138 and NIH grant NS09330 to. R. Seecof.