共查询到20条相似文献,搜索用时 15 毫秒
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Mónica Renedo Ignacio Arce Antonio Rodríguez Marta Carretero Lewis L. Lanier Miguel López-Botet E. Fernández-Ruiz 《Immunogenetics》1997,46(4):307-311
Natural killer (NK) cells preferentially express several type II glycoproteins of the calcium-dependent lectin superfamily.
The genes coding for these molecules are clustered on the distal mouse chromosome 6 and on the rat chromosome 4 in a region
designated the NK gene complex. To date, no definite evidence of the presence of a NK gene complex has been found in humans. Here we report the assignment by fluorescence in situ hybridization of the CD94 gene to human chromosome 12p12-p13, in the same region where the CD69 and NKG2A genes had been previously mapped. In addition, using a yeast artificial chromosome contig spanning this region we determined
that the human CD94, NKG2A, NKG2C, NKG2E, and NKR-P1A (NKR) genes map to the short arm of chromosome 12. The distal to proximal position of these loci are: NKR- CD69 - CD94/NKG2A/NKG2C/NKG2E. These data demonstrate the existence of a human NK gene complex located within a 5.6 cM interval flanked by the genetic markers D12S397 and D12S89. The physical distance spanned by the NK gene complex in humans ranges between 0.7 and 2.4 megabases.
Received: 17 January 1997 / Revised: 10 March 1997 相似文献
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Hans Nowack Eckhart Hahn Chella S. David Rupert Timpl Dietrich Götze 《Immunogenetics》1975,2(1):331-335
The genetically controlled immune response to calf skin collagen type I in mice could be demonstrated to be governed by at least two genes. One is linked to theH-2 complex and located within theIA subregion. High-responder alleles areH-2 b ,H-2 f , andH-2 s . The other gene(s) is not linked to theH-2 complex and high-responder allele(s) are found in the genome of B10 mice but not in the genome of DBA mice. There are strong indications that theIr-1A gene controls the response at the T-cell level, whereas it is assumed that the background gene(s) control the immune response at a different level. 相似文献
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NKG2D ligands (NKG2DLs) are a group of major histocompatibility complex (MHC) class I-like molecules, the expression of which is induced by cellular stresses such as infection, tumorigenesis, heat shock, tissue damage, and DNA damage. They act as a molecular danger signal alerting the immune system for infected or neoplastic cells. Mammals have two families of NKG2DL genes: the MHC-encoded MIC gene family and the ULBP gene family encoded outside the MHC region in most mammals. Rodents such as mice and rats lack the MIC family of ligands. Interestingly, some mammals have NKG2DL-like molecules named MILL that are phylogenetically related to MIC, but do not function as NKG2DLs. In this paper, we review our current knowledge of the MIC, ULBP, and MILL gene families in representative mammalian species and discuss the origin and evolution of the NKG2DL gene family. 相似文献
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ArecA clone was isolated from a cosmid library ofSerratia entomophila constructed in theEscherichia coli strain HB101. Subcloning and transposon mutagenesis were used to identify a 1.36 kb fragment containing therecA gene. A clonedrecA mutation, generated by transposon mutagenesis and the replacement of a portion of therecA gene with an antibiotic resistance cassette, was introduced into the chromosome via a marker exchange technique. TherecA strains created were deficient in DNA repair, homologous recombination and both the spontaneous and UV induction of prophages.S. entomophila recA strains showed continued pathogenicity towards the New Zealand grass grub,Costelytra zealandica. Simple procedures for further construction ofS. entomophila recA strains have been demonstrated. 相似文献
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N. Bonnefoy M. Kermorgant G. Dujardin P. Brivet-Chevillotte 《Molecular genetics and genomics : MGG》1996,251(2):204-210
TheSaccharomyces cerevisiae geneABC1 is required for the correct functioning of thebc 1 complex of the mitochondrial respiratory chain. By functional complementation of aS. cerevisiae abc1 ? mutant, we have cloned aSchizosaccharomyces pombe cDNA, whose predicted product is 50% identical to the Abc1 protein. Significant homology is also observed with bacterial, nematode, and even human amino acid sequences of unknown function, suggesting that the Abc1 protein is conserved through evolution. The cloned cDNA corresponds to a singleS. pombe geneabc1Sp, located on chromosome II, expression of which is not regulated by the carbon source. Inactivation of theabc1Sp gene by homologous gene replacement causes a respiratory deficiency which is efficiently rescued by the expression of theS. cerevisiae ABC1 gene. The inactivated strain shows a drastic decrease in thebc 1 complex activity, a decrease in cytochromeaa3 and a slow growth phenotype. To our knowledge, this is the first example of the inactivation of a respiratory gene inS. pombe. Our results highlight the fact thatS. pombe growth is highly dependent upon respiration, and thatS. pombe could represent a valuable model for studying nucleo-mitochondrial interactions in higher eukaryotes. 相似文献
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Mouse genomic DNA harboring the full coding sequence of cyclin G1 was cloned and analyzed. The locations of five coding exons and the intron–exon boundary sequences were found to be conserved between the mouse and the human genes. Two putative binding sites for thep53tumor suppressor gene product were found around the first exon: one was located in the 5′ regulatory region, and the other was in the first intron. The mouse cyclin G1 gene was mapped to bands A5 to B1 of chromosomes 11 (11A5–B1) by FISH using genomic DNA clone as a biotinylated probe. The location of mouse cyclin G1 is syntenic to that of its human homologue, which we previously mapped to 5q32–q34 of chromosome 5. An additional faint signal was detected on chromosome 4 (4B1–C2), probably indicating the presence of a cyclin G1-related gene or pseudogene in the mouse genome. 相似文献
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J. Sadowski P. Gaubier M. Delseny C. F. Quiros 《Molecular genetics and genomics : MGG》1996,251(3):298-306
We report the genetic and physical analysis by pulse field gel electrophoresis (PFGE) in threeBrassica diploid genomes for a cluster of five genes characterized in a selected segment of 15 kb on chromosome 3 ofArabidopsis thaliana, encoding aBradyrhizobium CycJ homologue (At1), a rat p67 translation factor homologue (At2), an Em-like (early methionine) protein (At3), chlorophyll synthase (At4) and a yeast Sac1 homologue (A5). TheArabidopsis gene array was found to be conserved on a single linkage group in each of theBrassica genomes. However, partial complexes were found to be duplicated in other chromosome segments on the same or other linkage groups. Some of the At genes, which could not be genetically mapped because of lack of polymorphism, were assigned to their respective linkage groups by physical mapping. The presence of multiple copies of theA. thaliana gene cluster in the threeBrassica genomes further establishes their complex nature, which results from extensive duplication and chromosomal rearrangement. In general, genetic distances between the At genes agreed with values expected for the physical distances determined inBrassica. 相似文献
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Assessing the utility of whole-genome amplified serum DNA for array-based high throughput genotyping
Kristine L Bucasas Gagan A Pandya Sonal Pradhan Robert D Fleischmann Scott N Peterson John W Belmont 《BMC genetics》2009,10(1):1-10
Background
Reproductive disorders and infertility are surprisingly common in the human population as well as in other species. The decrease in fertility is a major cause of cow culling and economic loss in the dairy herd. The conception rate has been declining for the past 30–50 years. Conception rate is the product of fertilization and embryonic survival rates. In a previous study, we have identified associations of several single nucleotide polymorphisms (SNPs) in the signal transducer and activator 5A (STAT5A) with fertilization and survival rates in anin vitro experimental system. The objectives of this study are to fine map theSTAT5A region in a search for causative mutations and to investigate the parent of origin expression of this gene.Results
We have performed a total of 5,222 fertilizations and produced a total of 3,696 in vitro fertilized embryos using gametes from 440 cows and eight bulls. A total of 37 SNPs were developed in a 63.4-kb region of genomic sequence that includesSTAT5A,STAT3, and upstream and downstream sequences of these genes. SNP153137 (G/C) in exon 8 ofSTAT5A was associated with a significant variability in embryonic survival and fertilization rate compared to all other examined SNPs. Expression analysis revealed thatSTAT5A is primarily monoallelically expressed in early embryonic stages but biallelically expressed in later fetal stages. Furthermore, the occurrence of monoallelic maternal expression ofSTAT5A was significantly higher in blastocysts, while paternal expression was more frequent in degenerative embryos.Conclusion
Our results imply thatSTAT5A affects embryonic survival in a manner influenced by developmental stage and allele parent of origin. 相似文献19.
M?nica Sawan Mendon?a Terezinha S Pera?olli Mário León Silva-Vergara Sílvio C Ribeiro Rafael Faria Oliveira Rinaldo Poncio Mendes Virmondes Rodrigues Jr 《Memórias do Instituto Oswaldo Cruz》2015,110(6):781-785
Paracoccidioidomycosis (PCM) is caused by dimorphic fungi from
theParacoccidioides brasiliensis complex. Previous studies have
demonstrated that the severity of disease is associated with a T-helper 2 immune
response characterised by high interleukin (IL)-4 production. In the present study we
analysed two polymorphisms in the IL-4 gene (-590 C/T and intron-3
microsatellite) in 76 patients with PCM and 73 control subjects from an endemic area.
The production of IL-4 by peripheral blood mononuclear cells after antigen or
phytohaemagglutinin stimulation was determined by ELISA. A significant correlation
was observed between the RP2/RP2 intron-3 genotype and infection with
Paracoccidioides sp. (p = 0.011), whereas the RP1/RP1 genotype
was correlated with resistance. No significant correlation was observed for
the IL-4 promoter polymorphism. Furthermore, the low IL-4
expression observed in the control group compared with patients was associated with
the RP1/RP1 genotype. These results suggest that IL-4polymorphisms
might be associated with the ability of the host to control Paracoccidioides
sp. infection. The relevance of this polymorphism is supported by the
observation that patients with disease produce high levels of IL-4 following mitogen
or antigen stimulation. The IL-4 gene is located in the cytokine
cluster region of chromosome 5 where other polymorphisms have also been
described. 相似文献