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Suppression of STAT5 Functions in Liver, Mammary Glands, and T Cells in Cytokine-Inducible SH2-Containing Protein 1 Transgenic Mice 总被引:18,自引:0,他引:18
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Akira Matsumoto Youichi Seki Masato Kubo Satoshi Ohtsuka Asuka Suzuki Itsuro Hayashi Kohichiro Tsuji Tatsutoshi Nakahata Masaru Okabe Shuichi Yamada Akihiko Yoshimura 《Molecular and cellular biology》1999,19(9):6396-6407
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A Biola P Lefebvre M Perrin-Wolff M Sturm J Bertoglio M Pallardy 《Molecular endocrinology (Baltimore, Md.)》2001,15(7):1062-1076
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Src kinases mediate STAT growth pathways in squamous cell carcinoma of the head and neck 总被引:9,自引:0,他引:9
Xi S Zhang Q Dyer KF Lerner EC Smithgall TE Gooding WE Kamens J Grandis JR 《The Journal of biological chemistry》2003,278(34):31574-31583
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Zhang H Conrad DM Butler JJ Zhao C Blay J Hoskin DW 《Journal of immunology (Baltimore, Md. : 1950)》2004,173(2):932-944
Adenosine is a purine nucleoside with immunosuppressive activity that acts through cell surface receptors (A(1), A(2a), A(2b), A(3)) on responsive cells such as T lymphocytes. IL-2 is a major T cell growth and survival factor that is responsible for inducing Jak1, Jak3, and STAT5 phosphorylation, as well as causing STAT5 to translocate to the nucleus and bind regulatory elements in the genome. In this study, we show that adenosine suppressed IL-2-dependent proliferation of CTLL-2 T cells by inhibiting STAT5a/b tyrosine phosphorylation that is associated with IL-2R signaling without affecting IL-2-induced phosphorylation of Jak1 or Jak3. The inhibitory effect of adenosine on IL-2-induced STAT5a/b tyrosine phosphorylation was reversed by the protein tyrosine phosphatase inhibitors sodium orthovanadate and bpV(phen). Adenosine dramatically increased Src homology region 2 domain-containing phosphatase-2 (SHP-2) tyrosine phosphorylation and its association with STAT5 in IL-2-stimulated CTLL-2 T cells, implicating SHP-2 in adenosine-induced STAT5a/b dephosphorylation. The inhibitory effect of adenosine on IL-2-induced STAT5a/b tyrosine phosphorylation was reproduced by A(2) receptor agonists and was blocked by selective A(2a) and A(2b) receptor antagonists, indicating that adenosine was mediating its effect through A(2) receptors. Inhibition of STAT5a/b phosphorylation was reproduced with cell-permeable 8-bromo-cAMP or forskolin-induced activation of adenylyl cyclase, and blocked by the cAMP/protein kinase A inhibitor Rp-cAMP. Forskolin and 8-bromo-cAMP also induced SHP-2 tyrosine phosphorylation. Collectively, these findings suggest that adenosine acts through A(2) receptors and associated cAMP/protein kinase A-dependent signaling pathways to activate SHP-2 and cause STAT5 dephosphorylation that results in reduced IL-2R signaling in T cells. 相似文献