婚姻地域距离及其变化的研究

对居住在新疆伊犁的１４９４对汉族夫妇进行了婚姻地域距离调查,并按不同年代、居住地、职业及文化程度分组进行了比较分析和统计学处理。结果表明,不同年代间、不同地域间、不同职业及不同文化程度间平均婚姻距离有显著性差异。     

吉林爆玉米遗传距离与杂种优势关系的研究

根据吉林省80个爆玉米自交系数量性状的表现,估算了它们之间的遗传距离。又估算了这80个自交系间随机组配的67个杂交组合F1代小区产量、爆粒率和膨胀倍数的杂种优势。相关和回归分析表明,F1代产量的对照优势与其双亲之间的遗传距离呈显著的二次曲线关系,而F1代品质特性爆粒率和膨胀倍数的对照优势与其双亲之间的遗传距离无确定性关系。因此,可以根据亲本间数量性状的综合遗传差异——遗传距离来预测吉林省爆玉米杂种一代的产量优势。 An experiment was conducted in 1990 to 1991 at the university,to study the relationships between genetic distance,cluster analysis and heterosis in popcorn(Zea mays var.everta).Results showed that there is no sense in cludtering parental lines for prediction of yield heterosis,but blood pedigree can beidentifed from cluster analysis.The relation between genetic divergence or distance and yield heterosis of F1 hybrids can be predicted from the genetic distances of their parental lines according to this model,but no definite relation could be found between parental genetic divergence and the quality heterosis of F1 hybrids.     

广州地区106例汉族人群MICA和MICB微卫星基因座 单体型研究 The Study on the Haplotype of MICA and MICB Microsatellite Locus In Guangzhou Han Population

利用聚合酶链反应和荧光（6－FAM）自动化检测技术对广东地区汉族106例无亲缘关系样本进行MICA基因外显子5和MICB基因内含子1微卫星基因座多态性及其单体型分布调查。根据群体资料估算两者间的单体型频率、连锁不平衡参数、相对连锁不平衡参数。结果显示，广州地区汉族人群MICA和MICB微卫星基因座基因型分布符合Hardy-Weinberg平衡法则，共检出MICA微卫星基因座 5个等位基因， MICB微卫星基因座14个等位基因。其中MICA A5基因频率最高（0.2877），A4基因频率最低（0.1321）。MICB CA14等位基因频率最高（0.3255），CA19、CA28等位基因频率最低（0.0047），未检出CA27。21种MICA－MICB单体型频率大于1%(连锁不平衡参数>0), 其中单体型A5－CA14 (16.73%), A5.1－CA18 (8.75%), A4－CA26(3.76%)，A9－CA15(3.66%)和A6－CA21(2.61%)为强连锁常见单体型（χ2>3.84, P<0.05）。广州地区汉族人群MICA和MICB微卫星基因座多态性和单体型分布有其自身特点，MICA和MICB微卫星基因座适合做为遗传标志，用于人类学、遗传疾病基因连锁分析、法医学亲子鉴定和个体识别等研究领域。Abstract: This study is to investigate genetic polymorphisms and haplotypes of microsatellite locus in the exon 5 of the MICA gene and intron 1 of the MICB gene based on 106 samples of Guangzhou Han Population by polymerase chain reaction and fluorescent technique (6-FAM). The corresponding haplotype frequencies, linkage disequilibria values and relative linkage disequilibria values were estimated based on population data. The results show that the genotype distributions of MICA and MICB microsatellite meet Hardy-Weinberg equilibrium in Guangdong Han population. In total, 5 alleles of MICA microsatellite locus and 14 alleles of MICB microsatellite locus were observed. MICA A5 was the most common allele (0.2877), whereas A4 was the least popular one (0.1321). MICB CA14 was the most common allele (0.3255), and CA19 and CA28 were the least popular ones (0.0047). CA27 was not observed. Twenty-one kinds of MICA-MICB haplotypes occurred at frequencies of more than 1% (linkage disequilibria value>0). The common MICA-MICB haplotypes were A5-CA14（16.73％）, A5.1- CA18 (8.75%), A4- CA26(3.76%)，A9-CA15(3.66%) and A6-CA21(2.61%)（χ2>3.84, P<0.05）, and they were strong linkage disequilibria. The polymorphisms and haplotypes distributions of MICA and MICB microsatellite locus in Guangzhou Han population have their own genetic characteristics. The microsatellite locus of the exon5 of the MICA gene and intron 1 of the MICB gene could be used as the genetic markers in the studies of anthropology, linkage analysis of genetic disease genes, individual identification and paternity test in forensic medicine.     

汉族左利人群的指纹研究

对111名长治市的汉族左利手者的指纹进行了分析研究, 并与138名右利手者组成的对照组进行了对比分析, 发现左利手组的双箕斗纹（Wd）显著高于对照组(P<0.005)。 Abstract　To study the distribution difference of fingerprint patterns between left-handed and normal populations of Han nationality in Changzhi area, We investigated the frequencies of fingerprint pattern combinations in one or two hands. Comparing the normal population (80 men and 58 women), the left-handed population (58 men and 53 women) posessed higher Wdrate (P<0.005).     

氯化钇和氯化镨引起的人淋巴细胞DNA分子损伤的研究 A Study of DNA Damage in Human Lymphocytes Induced by Yttrium Chloride and Praseodymium Chloride

用单细胞凝胶电泳法检测了稀土化合物氯化钇和氯化镨对人外周血淋巴细胞的DNA损伤效应。结果表明,与对照相比,3种不同浓度的氯化钇和氯化镨均可引起淋巴细胞DNA受损后DNA迁移率的显著升高,受损伤细胞的百分率与对照差异明显,提示氯化钇和氯化镨具有一定的遗传毒性。 Abstract:The effect of DNA damage in human lymphocytes induced by yttrium chloride and praseodymium chloride was detected using SCG assay.The results showed that a highly significant increase in DNA migration in DNA-damaged lymphocytes was induced by three different concentrations of yttrium and praseodymium compared with the control,The percentage of DNA-damaged have genetic toxicity.The relevant points to this study are discussed.     

番茄转录调控因子基因Pti5植物表达载体的构建及其转化烟草的研究 Construction of a Plant Expression Vector with Tomato Transcription Factor Gene Pti5 and Studies on Transgenic Tobaccos

本实验构建了含有CaMV35S启动子控制下的Pti5-VP16基因的植物双元表达载体pBI121UCH1。通过根癌农杆菌叶盘转化法,将Pti5-VP16基因导入烟草SRI中,经卡那霉素筛选,获得了抗性植株。经PCR和Southern印迹分析,表明抗性植株中整合了Pti5-VP16基因,经抗病性鉴定转基因烟草植株的抗病性明显提高。 Abstract:The plasmid pBI121UCH1 carrying Pti5-VP16 gene under the control of the cauliflower mosaic virus 35s promoter was constructed.Leaf segments of tobacco SRI were infected by Agrobacterium tumefaciens EHA105 with plasmid pBI121UCH1,from which kanamycin resistant plants were obtained.PCR and Southern analysis proved that the Pti5-VP16 gene was integrated into the genomes of the tobacco plants.The disease resistance assay showed that the disease resistance was enhanced in the transgenic tobacco plants.