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Genes encoding small GTP-binding proteins analogous to mammalian rac are preferentially expressed in developing cotton fibers 总被引:18,自引:0,他引:18
Deborah P. Delmer Julie R. Pear Andrawis Andrawis David M. Stalker 《Molecular & general genetics : MGG》1995,248(1):43-51
In animals, the small GTP-binding proteins, Rac and Rho, of theras superfamily participate in the signal rransduction pathway that regulates the organization of the actin cytoskeleton. We
report here on the characterization of two distinct cDNA clones isolated from a cotton fiber cDNA library that code for homologs
of animal Rac proteins. Using gene-specific probes, we have determined that amphidiploid cotton contains two genes that code
for each of the two Rac proteins, designated Rac13 and Rac9, respectively. The gene for Rac13 shows highly enhanced expression
in developing cotton fibers, with maximal expression occurring at the time of transition between primary and secondary wall
synthesis. This is also the time at which reorganization of the cytoskeleton occurs, and thus the pattern of expression of
Rac13 is consistent with its possible role, analogous to animal Rac, in the signal transduction pathway that controls cytoskeletal
organization. 相似文献
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Isolation and analyses of genes preferentially expressed during early cotton fiber development by subtractive PCR and cDNA array 总被引:29,自引:0,他引:29
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Ji SJ Lu YC Feng JX Wei G Li J Shi YH Fu Q Liu D Luo JC Zhu YX 《Nucleic acids research》2003,31(10):2534-2543
Cotton fibers are differentiated epidermal cells originating from the outer integuments of the ovule. To identify genes involved in cotton fiber elongation, we performed subtractive PCR using cDNA prepared from 10 days post anthesis (d.p.a.) wild-type cotton fiber as tester and cDNA from a fuzzless-lintless (fl) mutant as driver. We recovered 280 independent cDNA fragments including most of the previously published cotton fiber-related genes. cDNA macroarrays showed that 172 genes were significantly up-regulated in elongating cotton fibers as confirmed by in situ hybridization in representative cases. Twenty-nine cDNAs, including a putative vacuolar (H+)-ATPase catalytic subunit, a kinesin-like calmodulin binding protein, several arabinogalactan proteins and key enzymes involved in long chain fatty acid biosynthesis, accumulated to greater than 50-fold in 10 d.p.a. fiber cells when compared to that in 0 d.p.a. ovules. Various upstream pathways, such as auxin signal transduction, the MAPK pathway and profilin- and expansin-induced cell wall loosening, were also activated during the fast fiber elongation period. This report constitutes the first systematic analysis of genes involved in cotton fiber development. Our results suggest that a concerted mechanism involving multiple cellular pathways is responsible for cotton fiber elongation. 相似文献
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Tu LL Zhang XL Liang SG Liu DQ Zhu LF Zeng FC Nie YC Guo XP Deng FL Tan JF Xu L 《Plant cell reports》2007,26(8):1309-1320
Sea-island cotton (Gossypium barbadense L.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber
development mechanism has not been surveyed comprehensively. We constructed a normalized fiber cDNA library (from −2 to 25 dpa)
of G. barbadense cv. Pima 3-79 (the genetic standard line) by saturation hybridization with genomic DNA. We screened Pima 3-79 fiber RNA from
five developmental stages using a cDNA array including 9,126 plasmids randomly selected from the library, and we selected
and sequenced 929 clones that had different signal intensities between any two stages. The 887 high-quality expressed sequence
tags obtained were assembled into 645 consensus sequences (582 singletons and 63 contigs), of which 455 were assigned to functional
categories using gene ontology. Almost 50% of binned genes belonged to metabolism functional categories. Based on subarray
analysis of the 887 high-quality expressed sequence tags with 0-, 5-, 10-, 15-, and 20-dpa RNA of Pima 3-79 fibers and a mixture
of RNA of nonfiber tissues, seven types of expression profiles were elucidated. Furthermore our results showed that phytohormones
may play an important role in the fiber development.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Shengting Li Hao Chen Zhi Hou Yu Li Cuiling Yang Daojie Wang Chun‐Peng Song 《植物学报(英文版)》2020,62(7):998-1016
Cotton (Gossypium hirsutum L.) is a major crop and the main source of natural fiber worldwide. Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality, improved stress resistance of this crop plant is urgently needed. In this study, we used Gateway technology to construct a normalized full‐length cDNA overexpressing (FOX) library from upland cotton cultivar ZM12 under various stress conditions. The library was transformed into Arabidopsis to produce a cotton‐FOX‐Arabidopsis library. Screening of this library yielded 6,830 transgenic Arabidopsis lines, of which 757 were selected for sequencing to ultimately obtain 659 cotton ESTs. GO and KEGG analyses mapped most of the cotton ESTs to plant biological process, cellular component, and molecular function categories. Next, 156 potential stress‐responsive cotton genes were identified from the cotton‐FOX‐Arabidopsis library under drought, salt, ABA, and other stress conditions. Four stress‐related genes identified from the library, designated as GhCAS, GhAPX, GhSDH, and GhPOD, were cloned from cotton complementary DNA, and their expression patterns under stress were analyzed. Phenotypic experiments indicated that overexpression of these cotton genes in Arabidopsis affected the response to abiotic stress. The method developed in this study lays a foundation for high‐throughput cloning and rapid identification of cotton functional genes. 相似文献
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Verticillium dahliae transcription factor VdFTF1 regulates the expression of multiple secreted virulence factors and is required for full virulence in cotton
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Wen‐Qi Zhang Yue‐Jing Gui Dylan P. G. Short Ting‐Gang Li Dan‐Dan Zhang Lei Zhou Chun Liu Yu‐Ming Bao Krishna V. Subbarao Jie‐Yin Chen Xiao‐Feng Dai 《Molecular Plant Pathology》2018,19(4):841-857
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Suppression of GhAGP4 gene expression repressed the initiation and elongation of cotton fiber 总被引:2,自引:0,他引:2
Yunjing Li Diqiu Liu Lili Tu Xianlong Zhang Li Wang Longfu Zhu Jiafu Tan Fenglin Deng 《Plant cell reports》2010,29(2):193-202
Cotton fibers, important natural raw materials for the textile industry, are trichomes elongated from epidermal cells of cotton
ovules. To date, a number of genes have been shown to be critical for fiber development. In this study, the roles of genes
encoding fasciclin-like arabinoglactan proteins (FLAs) in cotton fiber were examined by transforming RNA interfering (RNAi)
construct. The RNAi according to the sequence of GhAGP4 caused a significant reduction of its mRNA level, and the expression of other three FLAs (GhAGP2, GhAGP3, GhFLA1) were also partially suppressed. The fiber initiation and fiber elongation were inhibited in the transgenic plants. As for
the mature fibers of transgenic cotton, the fiber length became significantly shorter and the fiber quality became worse.
In addition, the RNAi of GhAGP4 also affected the cytoskeleton network and the cellulose deposition of fiber cells. Through ovule culture, it was found that
the expression of cotton FLA genes were upregulated by GA3, especially for GhAGP2 and GhAGP4. These results indicate that the FLAs are essential for the initiation and elongation of cotton fiber development. 相似文献
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Cotton fiber is an extremely long plant cell. Fiber elongation is a complex process and the genes that are crucial for elongation
are largely unknown. We previously cloned a cDNA encoding an isoform of cotton profilin and found that the gene (designated
GhPFN1) was preferentially expressed in cotton fibers. In the present study, we have further analyzed the expression pattern of
GhPFN1 during fiber development and studied its cellular function using tobacco suspension cells as an experimental system. We report
that expression of GhPFN1 is tightly associated with fast elongation of cotton fibers whose growth requires an intact actin cytoskeleton. Overexpression
of GhPFN1 in the transgenic tobacco cells was correlated with the formation of elongated cells that contained thicker and longer microfilament
cables. Quantitative analyses revealed a 2.5–3.6 fold increase in total profilin levels and a 1.6–2.6 fold increase in the
F-actin levels in six independent transgenic lines. In addition to the effect on cell elongation, we also observed delayed
cell cycle progression and a slightly lower mitotic index in the transgenic cells. Based on these data, we propose that GhPFN1 may play a critical role in the rapid elongation of cotton fibers by promoting actin polymerization.
Hai-Yun Wang and Yi Yu contributed equally to this work. 相似文献
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A Gly65Val substitution in an actin,GhACT_LI1, disrupts cell polarity and F‐actin organization resulting in dwarf,lintless cotton plants
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Gregory N. Thyssen David D. Fang Rickie B. Turley Christopher B. Florane Ping Li Christopher P. Mattison Marina Naoumkina 《The Plant journal : for cell and molecular biology》2017,90(1):111-121
Actin polymerizes to form part of the cytoskeleton and organize polar growth in all eukaryotic cells. Species with numerous actin genes are especially useful for the dissection of actin molecular function due to redundancy and neofunctionalization. Here, we investigated the role of a cotton (Gossypium hirsutum) actin gene in the organization of actin filaments in lobed cotyledon pavement cells and the highly elongated single‐celled trichomes that comprise cotton lint fibers. Using mapping‐by‐sequencing, virus‐induced gene silencing, and molecular modeling, we identified the causative mutation of the dominant dwarf Ligon lintless Li1 short fiber mutant as a single Gly65Val amino acid substitution in a polymerization domain of an actin gene, GhACT_LI1 (Gh_D04G0865). We observed altered cell morphology and disrupted organization of F‐actin in Li1 plant cells by confocal microscopy. Mutant leaf cells lacked interdigitation of lobes and F‐actin did not uniformly decorate the nuclear envelope. While wild‐type lint fiber trichome cells contained long longitudinal actin cables, the short Li1 fiber cells accumulated disoriented transverse cables. The polymerization‐defective Gly65Val allele in Li1 plants likely disrupts processive elongation of F‐actin, resulting in a disorganized cytoskeleton and reduced cell polarity, which likely accounts for the dominant gene action and diverse pleiotropic effects associated with the Li1 mutation. Lastly, we propose a model to account for these effects, and underscore the roles of actin organization in determining plant cell polarity, shape and plant growth. 相似文献
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Wen-bin Liao Meng-bin Ruan Bai-ming Cui Nan-fei Xu Jia-ju Lu Ming Peng 《Plant Growth Regulation》2009,58(1):35-45
The aim of the investigation reported here was to assess the role of gibberellin in cotton fiber development. The results
of experiments in which the gibberellin (GA) biosynthesis inhibitor paclobutrazol (PAC) was tested on in vitro cultured cotton
ovules revealed that GA is critical in promoting cotton fiber development. Plant responses to GA are mediated by DELLA proteins.
A cotton nucleotide with high sequence homology to Arabidopsis thaliana
GAI (AtGAI) was identified from the GenBank database and analyzed with the BLAST program. The full-length cDNA was cloned from upland
cotton (Gossypium hirsutum, Gh) and sequenced. A comparison of the putative protein sequence of this cDNA with all Arabidopsis DELLA proteins indicated that GhRGL is a putative ortholog of AtRGL. Over-expression of this cDNA in Arabidopsis plants resulted in the dwarfed phenotype, and the degrees of dwarfism were related to the expression levels of GhRGL. The deletion of 17 amino acids, including the DELLA domain, resulted in the dominant dwarf phenotype, demonstrating that
GhRGL is a functional protein that affects plant growth. Real-time quantitative PCR results showed that GhRGL mRNA is highly expressed in the cotton ovule at the elongation stage, suggesting that GhRGL may play a regulatory role in cotton fiber elongation. 相似文献
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Isolation of a cotton CAP gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation 总被引:6,自引:0,他引:6
The cDNA encoding CAP (adenylyl cyclase-associated protein) was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA (GhCAP) contained an open reading frame that encoded 471 amino acid residues. RNA blot analysis showed that the cotton CAP gene was expressed mainly in young fibers. 相似文献
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The pentatricopeptide repeat (PPR) protein family is one of the largest and most complex families in plants. These proteins
contain multiple 35-amino acid repeats that are proposed to form a super helix capable of binding RNA. PPR proteins have been
implicated in many crucial functions broadly involving organelle biogenesis and plant development. In this study, we identified
many genes encoding PPR protein in Upland cotton through an extensive survey of the database of Gossypium hirsutum. Furthermore, we isolated five full-length cDNA of PPR genes from G. hirsutum 0-613-2R which were named GhPPR1–GhPPR5. Domain analysis revealed that the deduced amino acid sequences of GhPPR1–5 contained
from 5 to 10 PPR motifs and those PPR proteins were divided into two different PPR subfamilies. GhPPR1–2 belonged to the PLS
subfamily and GhPPR3–5 belonged to the P subfamily. Phylogenetic analysis of the five GhPPR proteins and 18 other plant PPR
proteins also revealed that the same subfamily clustered together. All five GhPPR genes were differentially but constitutively
expressed in roots, stems, leaves, pollens, and fibers based on the gene expression analysis by real-time quantitative RT-PCR.
This study is the first report and analysis of genes encoding PPR proteins in cotton. 相似文献
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Shinozuka H Hisano H Yoneyama S Shimamoto Y Jones ES Forster JW Yamada T Kanazawa A 《Molecular genetics and genomics : MGG》2006,275(4):399-408
A perennial ryegrass cDNA clone encoding a putative glycine-rich RNA binding protein (LpGRP1) was isolated from a cDNA library constructed from crown tissues of cold-treated plants. The deduced polypeptide sequence
consists of 107 amino acids with a single N-terminal RNA recognition motif (RRM) and a single C-terminal glycine-rich domain.
The sequence showed extensive homology to glycine-rich RNA binding proteins previously identified in other plant species.
LpGRP1-specific genomic DNA sequence was isolated by an inverse PCR amplification. A single intron which shows conserved locations
in plant genes was detected between the sequence motifs encoding RNP-1 and RNP-2 consensus protein domains. A significant
increase in the mRNA level of LpGRP1 was detected in root, crown and leaf tissues during the treatment of plants at 4°C, through which freezing tolerance is attained.
The increase in the mRNA level was prominent at least 2 h after the commencement of the cold treatment, and persisted for
at least 1 week. Changes in mRNA level induced by cold treatment were more obvious than those due to treatments with abscisic
acid (ABA) and drought. The LpGRP1 protein was found to localise in the nucleus in onion epidermal cells, suggesting that
it may be involved in pre-mRNA processing. The LpGRP1 gene locus was mapped to linkage group 2. Possible roles for the LpGRP1 protein in adaptation to cold environments are discussed. 相似文献