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1.
Three cultivars (cvs.) of Gladiolus hybridus Hort., namely ‘Her Majesty’, ‘Aldebaran’ and ‘Bright Eye’ were successfully micropropagated.
The cultures were established using intact cormels or segments of cormels and inflorescence axes on Murashige and Skoog (1962;
MS) medium. The response depended on media supplements; both callus formation or direct induction of shoot buds was observed.
Shoot differentiation from callus could be obtained on MS medium containing 1.0 μM BA (6–benzyladenine) and 10.0 μM NAA (α-naphthalene
acetic acid) in all three cultivars. The same could be achieved by giving a heat shock (HS; 50 °C, 1h) to callus cultures
(in case of ‘Her Majesty’ and ‘Aldebaran’ only) maintained on the basal medium. In these two cultivars, high sucrose concentration
(0.232, 0.290 or 0.348 M) also favoured growth and proliferation of shoot cultures on a plant growth regulator-free medium
at 20 °C in comparison to the cultures kept at 25 °C. On the other hand, shoot cultures maintained on the basal medium at
25 °C containing normal (0.058 M, i.e., 2.0%, w/v) sucrose concentration responded similar to those maintained at 20 °C on
a high sucrose medium; reduced response was observed on normal sucrose containing medium at 20 °C. Heat shock enhanced shoot
proliferation in the cultures maintained on basal medium, but induced prolific rooting in shoot cultures, within 5 days of
HS, on high sucrose (optimum 0.232 M) medium. While the number of roots increased at higher sucrose concentrations in the
medium in case of cvs. ‘Her Majesty’ and ‘Aldebaran’, the same was found to be independent of sucrose concentration in cv.
‘Bright Eye’. Generally the rooted plants produced on high sucrose (0.232 M) medium in comparison to medium with normal sucrose
concentration showed better survival (ca. 90% as against 40%) in the soil.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
The relationship between the induction of tracheary elementdifferentiation and exogenous L-methionine was examined in agar-growncultures of soya bean callus initiated from Glycine max L. Wayneand Clark 63. Although Wayne is a normal cultivarsoya bean, seedlings of Clark 63 exhibit abnormal growth at25 °C due to exessive ethylene biosynthesis at this temperature.Wayne callus showed increased xylogenesis in the presence ofexogenous L-methionine (3.7 µg 11) in comparisonto IAAKN controls at both 20 and 25 °C. Clark 63callus produced greater numbers of tracheary elements in responseto exogenous L-methionine only at 25 °C. The induction ofxylem differentiation was independent of the maintenance temperatureof the stock cultures of both cultivars. Xylogenesis initiatedbyan IAAKN medium was inhibited by the addition of AgNO3(20 mg 11) to the extent of 76.5 per cent in cv. Wayneand 6 per cent in cv. Clark 63. The inhibitory effect was partiallyreversed by the addition of L-methionine (3.7 µg 11)to the IAAKNAgNO2 medium. These data support thehypothesis that xylogenesis in vitro involves auxin, cytokininand ethylene. differentiation, xylogenesis, L-methionine, ethylene, Glycine max L., soya bean, callus culture, auxin, kinetin 相似文献
3.
Konstantin V. Kiselev Anna V. Turlenko Yuri N. Zhuravlev 《Plant Cell, Tissue and Organ Culture》2009,99(2):141-149
A somatic embryogenesis protocol for plant regeneration of northern red oak (Quercus rubra) was established from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium
(MS) containing 3% sucrose, 0.24% Phytagel™, and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d) after 4 weeks of culture in darkness. A higher response (66%) of embryogenic callus was induced on 0.45 μM 2,4-d. Higher numbers of globular- (31), heart- (17), torpedo- (12), and cotyledon-stage (8) embryos per explant were obtained
by culturing embryogenic callus on MS with 3% sucrose, 0.24% Phytagel™, and devoid of growth regulators after 8 weeks culture
in darkness. Continuous sub-culturing of embryogenic callus on medium containing 2,4-d yielded only compact callus. Desiccation of embryos for 3 days in darkness at 25 ± 2°C followed by cold storage at 4°C in
darkness for 8 weeks favored embryo germination and development of plantlets. Cotyledon-stage embryos subjected to desiccation
and chilling treatment cultured on MS with 3% sucrose, 0.24 Phytagel™, 0.44 μM 6-benzylaminopurine (BA), and 0.29 μM gibberellic
acid germinated at a higher frequency (61%) than with 0.44 μM BA alone and control cultures. Germinated plantlets developed
a shoot and root, were acclimatized successfully, and maintained in a growth room for plantlet development. 相似文献
4.
The development of a rapid protocol for high-efficiency somatic embryogenesis and plant regeneration from seed-derived embryogenic
callus cultures of California poppy (Eschscholzia californica Cham.) is reported. The optimized procedure required less than 13 weeks from the initiation of seed cultures to the recovery
of plantlets and involved the sequential transfer of cultures onto solid Murashige and Skoog basal medium containing three
different combinations of growth regulators. All steps were performed at 25 °C. Friable primary callus was induced from seeds
of E. californica cultured on medium supplemented with 1.0 mg l−1 2,4-dichlorophenoxyacetic acid. The primary callus was transferred to medium containing 1.0 mg l−1 1-naphthaleneacetic acid and 0.5 mg l−1 6-benzylaminopurine to establish embryogenic callus and promote somatic embryogenesis. Regenerated plantlets were recovered
after the conversion of somatic embryos on medium containing 0.05 mg l−1 6-benzylaminopurine and showed normal development. Embryogenic callus was induced at a frequency of 85%, an average of 45
somatic embryos were produced per callus, 90% of the somatic embryos converted, and about 70% of the plantlets were recovered
in soil. The growth rate of somatic embryo-derived shoots could be increased by gibberellic acid treatment, but the resulting
plantlets were hyperhydritic.
Received: 14 February 1999 / Revision received: 27 April 1999 / Accepted: 14 May 1999 相似文献
5.
Plant growth was assessed and cellular protein per nuclear DNAamount measured in root meristems and in callus derived fromembryos of a spring (Katepwa) and a winter variety(Beaver) of allohexaploid wheat exposed to lowtemperature treatment. The data obtained were used to test whetherthese genetically distinct varieties of wheat responded differentiallyto cold treatment. Seedlings were grown for 14 d at 20°Cand then transferred to 4°C for 14 d before returning themto 20°C, or else were maintained continuously at 20°C.In winter wheat, root growth at 4°C was significantly greaterover the first 7 d following transfer to 4°C compared withplants retained at 20°C, whereas in spring wheat it wasreduced at 4°C. The pattern of accumulation of cellularprotein for both root meristem cells and in callus cells wasalso generally enhanced at 4°C compared with 20°C inwinter wheat but not in spring wheat. Thus, clear inter-varietaldifferences were established both for dry weight accumulationand cellular protein, and the callus data clearly show thatthe low-temperature-induced accumulation of protein is a cellularphenomenon not necessarily linked to development. The extentto which cold-shock proteins are a component of this low temperature-inducedincrease in cellular protein is discussed. Copyright 2000 Annalsof Botany Company Protein, spring wheat, temperature, tissue culture, Triticum aestivum, winter wheat 相似文献
6.
A. L. Pinto-Sintra 《Plant Cell, Tissue and Organ Culture》2007,88(3):253-265
‘Touriga Nacional’ is the most important Portuguese grapevine cultivar used for Port wine, table wine and varietal wine production.
In order to obtain a reproducible plant regeneration system that allows the application of biotechnological tools to grapevine
breeding, embryogenic cultures were induced from immature flowers of three Touriga Nacional selected clones. Gynoecia and
anthers were cultured on Nitsch and Nitsch (Science 163:85–87, 1969) basal medium supplemented with four combinations of the
growth regulators 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-acetyl-l-aspartic acid (IASP), at 28°C, in the dark. Primary callus, observed on anthers and gynoecia in all media, produced embryogenic
callus when cultured on differentiation medium, at 24°C under light. The efficiency on induction of embryogenic callus ranged
from 1.2 ± 4.7% to 7.9 ± 13.8% in anthers, and from 17.9 ± 24.9% to 25.3 ± 22.9% in gynoecia. Seven lines of embryogenic cultures were established from the three clones. Multiplication of embryogenic
calluses was successfully obtained in maintenance medium, at 26°C, in the dark. These embryogenic calluses produced somatic
embryos when subcultured on differentiation medium, under a 16 h photoperiod. Somatic embryos were isolated and cultured on
germination medium to achieve conversion which ranged from 35.3 ± 48.5% to 72.7 ± 45.6%. The plantlets obtained were cultured in medium without growth regulators. Secondary embryogenesis
was also frequently observed in the hypocotyl-root transition region of somatic embryos. Although some morphological variation
occurred between somatic embryos, the regenerated plantlets had a normal phenotype. Maintenance of embryogenic cultures has
been achieved since 2002. 相似文献
7.
Callus and suspension cultures of Theobroma cacao L., initiatedfrom immature cotyledons of beans from pods harvested 120130days after pollination were established. A modified B-5 or MurashigeSkoogagar medium sustained growth of callus without loss of vigourafter each sub-culture. A 15-fold weight increase occurred duringthe 4 week culture periods at 30 ± 1 °C. Coconutwater improved callus growth substantially. The optimum hormonalconcentrations for growth of suspensions were 0.5 mg 11of 2, 4-dichlorophenoxyacetic acid and 0.1 mg I1 of kinetinin a MurashigeSkoog basal medium liquid medium. The optimumtemperature for growth of suspensions was 2530 °C.The cell number and cell mass of suspensions increased 20-foldin 14 days. No organogenesis or embryogenesis was observed. Theobroma cacao L., acao, cell culture, suspension culture, tissue culture. 相似文献
8.
A haploid callus line from anther cultures of the Asiatic hybrid lily ‘Connecticut King’ was maintained for a long term. The
survival and growth of the haploid calluses were affected by auxins of picloram, α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic
acid (2,4-D) and temperatures of 25, 15 and 7 °C during culture. Picloram was more suitable for maintenance of the haploid
calluses, whereas NAA and 2,4-D led to root and shoot formation from the haploid calluses. The best temperature for maintenance
was 25 °C. About 90% of cells in calluses were maintained in haploid level during 60 weeks of subculture, and about 80% of
cells were haploid in the calluses maintained over 2 years with the MS medium containing 4 μM picloram in the dark at 25 °C.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
9.
Summary
Cupressus macrocarpa and C. arizonica were examined for callus and cell culture production in vitro. Both species produced callus on agar-solidified MSCY medium supplemented with vitamins, antioxidants, 0.14 μM kinetin (KIN), and 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Suspension cultures of both species were established in liquid MSCY medium. Seiridin
(SE) and iso-seiridin (ISE), two phytotoxic butenolides produced by Seiridium cardinale, S. cupressi, and S. unicorne, the causal agents of many canker diseases of cypress, were tested on callus or cell suspension cultures. In the medium without
other plant growth regulators (PGR), SE promoted cell proliferation of cypress better than ISE, for callus initiation, callus
maintenance, and cell suspension cultures. The growth rates of cypress callus tissues and suspension cultures of both cypress
species on media containing 50–150 μM SE or ISE were measured. At concentrations of 50 μM and higher, growth rates increased exponentially with the SE concentration. A comparison with KIN and 2,4-D indicated that
50 μM SE promoted growth of callus tissues and cell suspension cultures more than 100 μM ISE. SE can also interact with, or counteract, KIN and 2,4-D. It was demonstrated that SE could replace KIN in the medium
for C. arizonica. SE could be involved in cell enlargement and proliferation processes. The less susceptible cypress species (C. arizonica) had a high content of terpenoids than that of the more susceptible species (C. macrocarpa). SE could be a useful tool as a phytohormonal-like regulator to manipulate physiological changes at the cellular level and
as an elicitor of sensitivity or tolerance of cypress germplasm to the phytotoxin. 相似文献
10.
Hisako Yamamoto 《Journal of plant research》1980,93(4):307-315
A bacteriostatic concentration of berberine much inhibited growth of the callus cultures ofLithospermum erythrorhizon, Datura inooxia andCarthamus tinctorius, but little inhibited the callus growth ofMacleaya cordata. On the other hand, the high concentration of berberine tended to stimulate the callus growth ofCoptis japonica var.japonica.
Among callus cultures of the five species described above, 4-desoxypyridoxine inhibited growth of the callus cultures ofD. innoxia andC. tinctorius. In these two callus cultures, recovery effects of some of the vitamin B6 group (10 μg/ml) on the inhibition of callus growth by berberine (100 μg/ml) or 4-desoxypyridoxine (50 μg/ml) were observed. 相似文献
11.
ADKINS STEPHEN W.; KUNANUVATCHAIDACH RATCHANEE; GRAY SIMONE J.; ADKINS ANDREA L. 《Journal of experimental botany》1993,44(12):1829-1835
Modifications to the gaseous envelope by callus during culturein Petri dishes were shown to reduce growth and promote necrosisof several rice (Oryza sativa L.) cultivars. Incubatingcallusunder a continuous flow of gas mixtures of known compositionsuggested that the inhibition of growth was caused by the accumulationof ethylene, the depletion of oxygen and, to a lesser extent,the accumulation of carbon dioxide. In order to evaluate theimportance of ethylene accumulation aminoethoxyvinylglycine(AVG), 1-aminocyclopropane-l-carboxylic acid (ACC and silvernitrate (AgNO3), were added to the nutrient medium and ethylenemeasurements performed during callus culture. Ethylene restrictedcallus growth particularly under high (35 °C) as comparedto moderate (25 °C) temperatures and under illuminated ascompared to darkened incubation. Under illuminated incubationat 25 °C AVG (5 mmol m3) and AgNO°(50 mmol m3)significantly improvedcallus growth (100 and 60% respectively)while ACC (200 mmol m3) significantly decreased growth(40%). AVG and AgNO3 were less effective under dark incubationat 25 °C where ethylene production was lower. Furthermore,callus growth was significantly better in large as comparedto small culture vessels since the ethylene concentration wasdiluted and more oxygen was available for respiration. Bettercontrol of ethylene and increased oxygen availability couldbe a way ofproducing healthy callus for the formation of embryogenictissues of otherwise recalcitrant cultivars of rice (e.g. IndicaIR42) and may be a way of improving manipulation of other cerealspecies. Key words: 1-Aminocyclopropane-1-carboxylic acid, aminoethoxyvinylglycine, callus, ethylene, Oryza sativa, silver nitrate 相似文献
12.
We applied metabolic control analysis to the Kennedy pathway for triacylglycerol formation in tissue cultures from the important oil crops, olive (Olea europaea L.) and oil palm (Elaeis guineensis Jacq.). When microsomal fractions were incubated at 30 degrees C rather than 20 degrees C, there was an increase in triacylglycerol labelling. This increase was accompanied by a build up of diacylglycerol (DAG) radioactivity in olive but not in oil palm, suggesting that the activity of DAG acyltransferase (DAGAT) was becoming limiting in olive. We used 2-bromooctanoate as a specific inhibitor of DAGAT and showed that the enzyme had a flux control coefficient under the experimental conditions of 0.74 in olive but only 0.12 in oil palm. These data revealed important differences in the regulation of lipid biosynthesis in cultures from different plants and suggest that changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops. 相似文献
13.
Temperature-stress Pretreatment in Barley Anther Culture 总被引:7,自引:0,他引:7
Methods of pretreating anthers at different temperatures priorto culture have been tested, with respect to pollen-callus productionand plant regeneration, in Hordeum vulgare cv. Sabarlis. For callus production, pretreatment of excised spikes (in sealedPetri dishes) was more effective than pretreatment of excisedtillers (in water or in polythene) at both 4 and 25 °C.Pretreatment of individual anthers at these temperatures wasdeleterious. Greater callus yields resulted from pretreatmentat 4 than at 25 °C, both for spikes and tillers, 35weeks being required for maximal yields at 4 °C and 35days at 25 °C. At 4 °C, a shorter pretreatment was requiredfor spikes than for tillers. Pretreatment of spikes was alsomore effective at 4 than at 7, 14 or 20 °C. Pretreatmentof individual spikelets at 4 °C was as effective as thatof whole spikes. For plant regeneration, calluses derived from pretreatment ofspikes were more effective than those derived from pretreatmentof tillers. More plants resulted from pretreatment at 4 thanat 25 °C, both for spikes and tillers. Maximal pretreatmenttimes for plant regeneration generally exceeded those for callusproduction. Following spike pretreatment at 4 °C the maximumfor plant regeneration exceeded that for callus production byabout 2 weeks. With this optimal pretreatment approximately60 per cent of the calluses gave rise to plantlets. Among this60 per cent, for every three calluses giving albinos, two gavegreen plantlets, equivalent to five green plantlets on averagefor every 100 anthers (= two spikes) cultured. The ratio ofgreen to albino plantlets was lower for all other pretreatments. Hordeum vulgare L., barley, anther culture, pollen callus, pollen plant-production, temperature stress 相似文献
14.
Olga Molchan Svetlana Romashko Vladimir Yurin 《Plant Cell, Tissue and Organ Culture》2012,108(3):535-539
l-tryptophan decarboxylase (TDC, EC 4.1.1.28) catalyses the formation of tryptamine from tryptophan, and therefore it plays
a role in terpenoid indole alkaloids biosynthesis. In this study, TDC activity and tryptamine accumulation were monitored
in callus cultures of important medicinal plant Vinca minor L. Callus cultures, established from leaf tissues, were incubated on Murashige and Skoog (MS) medium supplemented with 4.4 μM
kinetin and different concentrations (0.44, 1.1, 2.2, 4.4 and 6.6 μM) of naphthaleneacetic acid (NAA), and grown either in
the dark or under 16 h photoperiod. When the basal enzyme activity of TDC was determined in these cultures, it was 0.5–0.7 nmol
tryptamine mg−1 prot. min−1. Moreover, this activity remained linear over time and over protein concentrations, and with optimum pH levels between 6.5
and 7.5, and an optimum temperature of 35°C. The Michaelis–Menten constant (Km) for l-tryptophan was 1.3 mM. TDC cofactor, pyridoxal-5′-phosphate (1 mM), increased the enzyme activity. During later stages of
callus culture growth cycle, an increase in TDC activity was observed, and this activity depended on culture conditions and
age of callus cultures. In addition, TDC activity and tryptamine accumulation in callus cultures were strongly enhanced by
light treatment. 相似文献
15.
Palmero Llamas D de Cara Gonzalez M Iglesias Gonzalez C Ruíz Lopez G Tello Marquina JC 《Journal of industrial microbiology & biotechnology》2008,35(11):1405-1409
The mycelial growth of 18 Fusarium solani strains isolated from sea beds of the south-eastern coast of Spain was tested on potato-dextrose-agar adjusted to different
osmotic potentials with either KCl or NaCl (−1.50 to −144.54 bars) in 10 °C intervals ranging from 15 to 35 °C. Fungal growth
was determined by measuring colony diameter after 4 days of incubation. Mycelial growth was maximal at 25 °C. The quantity
and frequency pattern of mycelial growth of F. solani differ significantly at 15 and 25 °C, with maximal growth occurring at the highest water potential tested (−1.50 bars); and
at 35 °C, with a maximal mycelial growth at −13.79 bars. The effect of water potential was independent of salt composition.
The general growth pattern of F. solani showed declining growth at potentials below −41.79 bars. Fungal growth at 35 °C was always higher than that grow at 15 °C,
of all the water potentials tested. Significant differences observed in the response of mycelia to water potential and temperature
as main and interactive effects. The viability of cultures was increasingly inhibited as the water potential dropped, but
some growth was still observed at −99.56 bars. These findings could indicate that marine strains of F. solani have a physiological mechanism that permits survival in environments with low water potential. The observed differences in
viability and the magnitude of growth could indicate that the biological factors governing potential and actual growth are
affected by osmotic potential in different ways. 相似文献
16.
Role of temperature as a triggering signal for organogenesis or somatic embryogenesis in wounded leaves of chicory cultured in vitro 总被引:3,自引:0,他引:3
Decout E.; Dubois T.; Guedira M.; Dubois J.; Audran J.-C.; Vasseur J. 《Journal of experimental botany》1994,45(12):1859-1865
Static liquid half-strength Murashige and Skoog medium, containing10.1 mM KCI instead of KNO3, 1.7 mM glutamine, microelements,vitamins, 60 mM sucrose, 0.1 //M a-naphthaleneacetic acid and2.5//M 2-isopentenyladenine allows either somatic embryo-genesisor shoot organogenesis along the borders of leaf incisions ofa Cichorium hybrid [Cichorium intybus L. x Cichorium endiviaL.). These phenomena are temperature-dependent and the latterpromotes the development of callus and shoots at 20 °C and25 °C instead of direct somatic embryogenesis at 35 °C.At 30 °C all types of morphogenesis are observed. After5 d of culture, cells grown at each temperature exhibit enlargednuclei with prominent nucleoli, fragmented vacuoles and densecytoplasm. However, at 25 °C, callose is restricted to woundedcells whereas at 35 °C some nearby mesophyll cells showa more or less complete callose sheath. On the 7th day, proembryosat 35 °C show a superficial network which is absent at 25°C on shoot primordia which are covered with a smooth precociousproto-derm. Why do activated cells undergo segmentation andsomatic embryogenesis at 35 °C instead of ordinary mitosiswith callus and shoot formation at 20 °C and 25 °C? Key words: Cichorium, morphogenesis, somatic embryos, tpmnpratnrp 相似文献
17.
Hypocotyl and cotyledon segments of Clianthus formosus were cultured on a modified deFossard medium M supplemented with cytokinins. 62% of cultures on medium with 20 μM BA produced
callus which subsequently gave rise to shoots. 40% of shoots excised from callus produced roots after transfer to auxin-rich
media (20 μM NAA or 10 μM IBA+10 μM NAA). Root production was enhanced following a 7-day dark treatment. 32% of nodes from
mature plants produced multiple shoots on 2 μM BA+2 μM KIN. 30% of these shoots rooted on medium without hormones. 70% of
rooted plantlets were successfully transferred to potting medium and glasshouse conditions. A period of cold treatment (10
days at 5°C) reduced vitrification from 68 to 22% of cultures. 相似文献
18.
Jolanta Biesaga-Kocielniak Janusz Kocielniak Maria Filek Anna Janeczko 《Plant Cell, Tissue and Organ Culture》2008,94(2):139-147
The aim of this study was to produce suspension cultures of winter wheat directly from immature embryos bypassing the callus
stage, and to determine their capacity for growth and regeneration in comparison to suspension cultures produced from callus.
The study was carried out using Polish winter wheat varieties: ‘Grana’ and ‘Rosa’. Immature embryos were isolated, homogenized
and transferred directly to liquid medium supplemented with 2,4-D. Actively dividing cell cultures were obtained within 2 months
after the cultures were started. Suspension cultures from callus of immature embryos was also produced. With both cultivars,
faster growth was observed in the suspension cultures produced directly from embryos than in the suspensions produced from
callus. Metabolic activity was higher in the suspension culture produced directly from embryos than in the suspension derived
from callus only in ‘Grana’. The production of 1-amiocyclopropane-1-carboxylic acid (ACC), an ethylene precursor, was lower
in the suspension cultures produced directly from embryos than in the suspensions produced from callus. Morphogenic capacity
was significantly higher in aggregates derived directly from embryos than in aggregates derived from callus. With ‘Rosa’,
about one third of the aggregates derived directly from embryos regenerated shoots. Production of ACC was lower in ‘Rosa’
cell culture that regenerated then in other cell cultures that did not. Photosystem II reactions were more efficient in dark
green aggregates than in light green or pale green aggregates which were unable to regenerate. With the method presented,
wheat cell suspension cultures with a regeneration potential can be produced in 2 or 3 months less time than with traditional
methods. 相似文献
19.
Summary Callus cultures of Adhatoda zeylanica Medicus were established from leaf and petiole explants. Accumulation of a bioactive pyrroloquinazoline alkaloid, vasicine,
in callus cultures was detected and confirmed by thin layer chromatography, electron-ionization mass spectra, 13C NMR and high-pressure liquid chromatography analysis. The mass of vasicine obtained from leaf-derived callus cultures was
found as 188 and this is comparable to that of the authentic sample. The retention time for leaf-derived extract was 10.065
and for the petiole-derived extract was 9.78 (authentic sample had 9.6 retention time) on high-performance liquid chromatography.
The mass and NMR spectra were compared with the spectra obtained from the authentic sample of vasicine. Different growth regulators
greatly influenced the growth of callus cultures. The accumulation of vasicine was more in leaf-derived callus grown on Murashige
and Skoog (MS) medium with 2.3 μM kinetin, and 4.5 μM 2,4-dichlorophenoxyacetic acid. This is the first report on in vitro production of a pharmacologically important compound vasicine and its characterization by mass spectrometry and 13C NMR studies from callus cultures of Adhatoda zeylanica. 相似文献
20.
WILLIAM F. SHERIDAN 《Physiologia plantarum》1975,33(2):151-156
Callus induction and subculture was successful with mature embryos and stem sections of seedlings of Zea mays L. on Linsmaier and Skoog's medium modified to contain 4 mg/I of 2,4-D and 1 g/I of casamino acids. — 2,4-D was superior to NAA and IAA for both callus induction and growth. Callus subcultured on NAA formed abundant roots on agar-solidified media and numerous root-like primordia in liquid cultures. — Kinetin had no effect on callus induction in the presence of 2,4-D and neither kinetin nor gibberellic acid stimulated callus growth during subculture. — Callus grew equally well on the medium of Linsmaier and Skoog, that of Schenk and Hildebrandt, and the B-5 medium of Gamborg and Eveleigh containing 2% sucrose, 4 mg/I of 2,4-D and 1 g/I of casamino acids. — The callus grew more rapidly at 25°C than at 30°C or 35°C. Little difference was noted at any temperature in callus growth in alternating light (16 h) and dark (8 h) or continuous dark. — Sucrose was superior to glucose and maltose in both liquid and agar-solidified cultures. Lactose and galactose failed to support callus growth. 相似文献