共查询到20条相似文献,搜索用时 31 毫秒
1.
《Harmful algae》2015
A differential screening study using high-resolution (HR)-hydrophilic interaction chromatography (HILIC)-electrospray ionization (ESI)–quadrupole time-of-flight mass spectrometry (Q-TOF MS) was conducted to identify saxitoxin (STX) analogues in the marine dinoflagellate toxic sub-clone Alexandrium tamarense Axat-2 and the non-toxic sub-clone UAT-014-009 derived from the same Japanese isolate. One unknown compound was identified only in the toxic sub-clone and was found to have the molecular formula C9H16N6O2. This structure differed from that of decarbamoyl STX (dcSTX; C9H16N6O3) by the loss of a single oxygen. A 12-deoxy-dcSTX standard (a mixture of 12α- and β-deoxy-dcSTX) was chemically prepared from dcSTX by reduction with sodium borohydride. The unknown compound in the toxic strain of A. tamarense was identified as 12β-deoxy-dcSTX by comparison of its HR-HILIC-LC–MS retention time and HR–MS/MS spectrum with those of the chemically prepared standard, and the identification was confirmed by high-sensitivity HPLC analysis with post-column fluorescent derivatization. Moreover, two Japanese isolates of A. catenella showing toxin profiles different from that of A. tamarense were also found to contain 12β-deoxy-dcSTX. Previously, 12β-deoxy-dcSTX was isolated from the freshwater cyanobacterium Lyngbya wollei, which produces a unique set of STX analogues. This study is the first evidence of the presence of 12β-deoxy-dcSTX in marine dinoflagellates. 相似文献
2.
Marc Seifert Glenn McGregor Geoff Eaglesham Wasantha Wickramasinghe Glen Shaw 《Harmful algae》2007,6(1):73-80
Lyngbya wollei (Farlow ex Gomont) Speziale and Dyck is a common mat-forming benthic cyanobacterium from freshwater habitats. We found that two populations from southeast Queensland (Australia) produce the potent cyanotoxin cylindrospermopsin (CYN) and its analogue, deoxy-cylindrospermopsin (deoxy-CYN). The highest concentrations in environmental samples were 20 and 550 μg g−1 dry weight for CYN and deoxy-CYN, respectively. A sub-sample maintained in culture for over 16 months yielded concentrations of 33 and 308 μg g−1 dry weight for CYN and deoxy-CYN, respectively. The concentration of deoxy-CYN in L. wollei was 10–300 times higher than CYN, suggesting that, unlike many other CYN-producing cyanobacteria, the primary compound produced by L. wollei is deoxy-CYN. The production of CYN and deoxy-CYN by L. wollei represents a potential human health risk and an additional source of these toxins in freshwaters. This is the first report of the production of CYN and deoxy-CYN by L. wollei or any species of the Oscillatoriales. 相似文献
3.
4.
《Harmful algae》2016
The Makah Tribe of Neah Bay, Washington, has historically relied on the subsistence harvest of coastal seafood, including shellfish, which remains an important cultural and ceremonial resource. Tribal legend describes visitors from other tribes that died from eating shellfish collected on Makah lands. These deaths were believed to be caused by paralytic shellfish poisoning, a human illness caused by ingestion of shellfish contaminated with saxitoxins, which are produced by toxin-producing marine dinoflagellates on which the shellfish feed. These paralytic shellfish toxins include saxitoxin, a potent Na+ channel antagonist that binds to the pore region of voltage gated Na+ channels. Amino acid mutations in the Na+ channel pore have been demonstrated to confer resistance to saxitoxin in softshell clam populations exposed to paralytic shellfish toxins present in their environment. Because of the notion of resistance to paralytic shellfish toxins, the study aimed to determine if a resistance strategy was possible in humans with historical exposure to toxins in shellfish. We collected, extracted and purified DNA from buccal swabs of 83 volunteer Makah tribal members and sequenced the skeletal muscle Na+ channel (Nav1.4) at nine loci to characterize potential mutations in the relevant saxitoxin binding regions. No mutations of these specific regions were identified after comparison to a reference sequence. This study suggests that any resistance of Makah tribal members to saxitoxin, if present, is not a function of Nav1.4 modification, but may be due to mutations in neuronal or cardiac sodium channels, or some other mechanism unrelated to sodium channel function. 相似文献
5.
Many algal toxins target voltage-gated sodium (Na+) channels where they act to block or otherwise perturb the function of this critical component of an animal’s neuromuscular system. However, many eukaryotes, particularly those that are routinely exposed to algal toxins, have toxin-insensitive or toxin-resistant Na+ channels. Here we provide a brief overview of the structure, function and evolution of voltage-gated Na+ channels, and the structural basis for toxin-insensitivity, as a prelude to a discussion of whether the early evolution of Na+ channels may have been influenced by the presence of algal toxins in the environment. 相似文献
6.
《Biomass》1988,15(4):249-257
An experiment was conducted in the growth chamber to quantify the biomass production, N removal and N2 fixation from a synthetic medium by Chlamydomonas reinhardtii and Anabaena flos-aquae. Nitrogen was supplied at a concentration of 100 mg liter−1 of NO3−−15N and NH4+−15 (3·5 atom %), respectively. After 21 days Chlamydomonas reinhardtii removed an average of 83·8 and 78·7 mg N liter−1 as NO3− and NH4+, respectively. Averages of 0·89 and 0·71 g liter−1 (first batch), 1·63 and 0·95 g liter− (second batch) algal biomass were collected from NO3− and NH4+ media, respectively. Uptake rates of 0·11 mg 15N g−1 algae day−1 from NO3− medium and 0·10 mg 15N g−1 algae day−1 from NH4+ medium were calculated. Algal cells grown in NO3− and NH4+ medium contained 71 and 65 g N kg−1 (first batch), 39 and 58 g N kg−1 (second batch), respectively. Anabaena flos-aquae produced averages of 0·58 and 0·46 g liter−1 (first batch), 0·55 and 0·48 g liter−1 (second batch) after 14 days of growth from NO3− and NH4+ media, respectively. Blue-green algal biomass contained higher N (81–98 g kg−1) than green algae. Isotope dilution method for determining N2 fixation indicated that 55% and 77% of total N of blue-green algae grown in NO3− and NH4+ media, respectively, was derived from the atmosphere. 相似文献
7.
Sean A. King 《Hydrobiologia》2014,734(1):27-37
Hydrodynamic properties of rivers and streams are primary controls on the abundance and structure of algal communities. In Florida, USA, filamentous macroalgal proliferation in spring-fed rivers has largely been attributed to nitrogen enrichment; however, changes in hydrodynamics and other potential mechanisms have not been thoroughly evaluated. This study tested whether macroalgal abundance (primarily Lyngbya wollei) was related to hydrodynamics in Gum Slough, a sub-tropical Florida spring-fed river. Flow velocities and macroalgal cover were measured at three transects bimonthly between June 2010 and January 2013, a period during which river discharge fluctuated in response to a drought and a tropical storm. Temporal variation in macroalgal cover was significantly inversely related to flow velocity, excluding fall/winter sampling events when light limitation was likely. The results indicate a velocity threshold below which macroalgal cover increased substantially, and a lower threshold below which complete cover frequently occurred. Local differences in channel morphology and substrate type (i.e., aquatic plant beds) seemed to affect the relationship between macroalgal cover and velocity at each transect. Nitrogen and phosphorus concentrations and other water chemistry parameters were not associated with variation in macroalgal cover. Overall, this study suggests that hydrodynamics can strongly control filamentous macroalgae in Florida spring-fed rivers. 相似文献
8.
Junichi Maruyama Tamao Noguchi Shigeki Matsunaga Kanehisa Hashimoto 《Bioscience, biotechnology, and biochemistry》2013,77(11):2783-2788
The application of fast atom bombardment- and secondary ion-mass spectrometry to the determination of molecular weights of non-volatile toxins was successfully examined with the use of authentic specimens of paralytic shellfish poisons (saxitoxin, gonyautoxin-1, gonyautoxin-2 and protogonyautoxin-1) and tetrodotoxin.Two non-sulfated toxins, saxitoxin and tetrodotoxin, produced intense pseudomolecular ion peaks in both positive and/or negative ion detection. On the other hand, each of gonyautoxin-1 and -2 and protogonyautoxin-1, all sulfated, gave only small (M + H)+ ion peaks in the positive ion detection whereas exhibiting prominent (M – H)? ion peaks in the negative ion spectrum.The molecular weights thus determined were 299 for saxitoxin, 411 for gonyautoxin-1, 395 for gonyautoxin-2, 475 for protogonyautoxin-1 and 319 for tetrodotoxin, in good accordance with their molecular weights. 相似文献
9.
《Harmful algae》2015
Despite their potential impact on phytoplankton dynamics and biogeochemical cycles, biological associations between algae and bacteria are still poorly understood. The aim of the present work was to characterize the influence of bacteria on the growth and function of the dinoflagellate Alexandrium tamarense. Axenic microalgal cultures were inoculated with a microbial community and the resulting cultures were monitored over a 15-month period, in order to allow for the establishment of specific algal–bacterial associations. Algal cells maintained in these new mixed cultures first experienced a period of growth inhibition. After several months, algal growth and cell volume increased, and indicators of photosynthetic function also improved. Our results suggest that community assembly processes facilitated the development of mutualistic relationships between A. tamarense cells and bacteria. These interactions had beneficial effects on the alga that may be only partly explained by mixotrophy of A. tamarense cells. The potential role of organic exudates in the establishment of these algal–bacterial associations is discussed. The present results do not support a role for algal–bacterial interactions in dinoflagellate toxin synthesis. However, variations observed in the toxin profile of A. tamarense cells during culture experiments give new clues for the understanding of biosynthetic pathways of saxitoxin, a potent phycotoxin. 相似文献
10.
The ability of the benthic cyanobacterium Lyngbya wollei to fix nitrogen was studied using field samples and axenic cultures. L. wollei was collected and isolated from Lake Okeechobee, Florida, where it forms extensive mats. Rates of acetylene reduction up
to 39.1 nmol mg dry wt−1 h−1 were observed for field samples. The maximum observed rate of acetylene reduction in axenic laboratory cultures was 200 nmol
mg dry wt−1 h−1. Aerobic conditions limited nitrogen fixation activity, but dark/light cycles promoted the development of activity. Reduced
oxygen levels appeared to be required for the development of significant levels of nitrogenase activity. The level of irradiance
also had a significant impact on the level of activity. The potential significance of nitrogen fixation to Lyngbya production is discussed. 相似文献
11.
Ammonium and nitrate uptake rates in the macroalgae Ulva fenestrata (Postels and Ruprecht) (Chlorophyta) and Gracilaria pacifica (Abbott) (Rhodophyta) were determined by 15N accumulation in algal tissue and by disappearance of nutrient from the medium in long‐term (4–13 days) incubations. Nitrogen‐rich algae (total nitrogen> 4% dry weight [dw]) were used to detect isotope dilution by release of inorganic unlabeled N from algal thalli. Uptake of NH4 + was similar for the two macroalgae, and the highest rates were observed on the first day of incubation (45 μmol N·g dw ? 1·h ? 1 in U. fenestrata and 32 μmol N·g dw ? 1·h ? 1 in G. pacifica). A significant isotope dilution (from 10 to 7.9 atom % enrichment) occurred in U. fenestrata cultures during the first day, corresponding to a NH4 + release rate of 11 μmol N·g dw ? 1·h ? 1. Little isotope dilution occurred in the other algal cultures. Concurrently to net NH4 + uptake, we observed a transient free amino acid (FAA) release on the first day in both macroalgal cultures. The uptake rates estimated by NH4 + disappearance and 15N incorporation in algal tissue compare well (82% agreement, defined as the percentage ratio of the lower to the higher rate) at high NH4 + concentrations, provided that isotope dilution is taken into account. On average, 96% of added 15NH4 + was recovered from the medium and algal tissue at the end of the incubation. Negligible uptake of NO3 ? was observed during the first 2–3 days in both macroalgae. The lag of uptake may have resulted from the need for either some N deprivation (use of NO3 ? pools) or physiological/metabolic changes required before the uptake of NO3 ? . During the subsequent days, NO3 ? uptake rates were similar for the two macroalgae but much lower than NH4 + uptake rates (1.97–3.19 μmol N·g dw ? 1·h ? 1). Very little isotope dilution and FAA release were observed. The agreement between rates calculated with the two different methods averaged 91% in U. fenestrata and 95% in G. pacifica. Recovery of added 15NO3 ? was virtually complete (99%). These tracer incubations show that isotope dilution can be significant in NH4 + uptake experiments conducted with N‐rich macroalgae and that determination of 15N atom % enrichment of the dissolved NH4 + is recommended to avoid poor isotope recovery and underestimation of uptake rates. 相似文献
12.
Two species of benthic damselfishes from the Gulf of California, Mexico, use contrasting behaviors when feeding on benthic algal communities. The small (±70 g) Cortez damselfish, Eupomacentrus rectifraenum (Gill, 1862), feeds selectively from a multi-species algal mat, eats fleshy red and green algae and ignores brown and calcareous algae. The giant blue damselfish, Microspathodon dorsalis (Gill, 1862), is a large (±450 g), lethargic, nonselective feeder which grazes on a near monoculture of a fleshy red alga, Polysiphonia sp. Feeding activity for both species is low in the morning peaks during late afternoon, and drops sharply as night approaches. Based on feeding rates, gut-filling times, and weights of gut contents, Cortez damselfish process six to eight full guts of food and giant blue damselfish three full guts of food per day. The algal mat exhibits high standing crops (291–618 g dry wt · m?2) and low productivity, but the preferred food of the Cortez damselfish (Ulva) appears to colonize the mat frequently and grow rapidly. The Polysiphonia dominated community on giant blue damselfish territories exhibits low standing crops (23 g · m?2) and high productivity (34–47 times that of the mat per gram algae). Even though the feeding behaviors and resources used by the two damselfishes differ, both species eat similar food (delicate red and green fleshy algae, and depend on rapid colonization and/or high productivity to maintain their primary foods in the grazed algal community. 相似文献
13.
《Harmful algae》2013
Florida is a hotspot for cyano- and microalgal harmful algal blooms (HABs) with annual red-tide events off-shore and blooms of Lyngbya spp. commonly observed in both marine and freshwater environments. This region also provides extensive foraging habitat for large populations of herbivorous green turtles (Chelonia mydas) and manatees (Trichechus manatus latirostris). The exposure of aquatic organisms to HAB toxins is not well known and whilst acute exposures are better understood, the vulnerability of aquatic animals to chronic exposure from multiple HAB toxins over prolonged periods has rarely been addressed. This study aimed to identify the presence of toxic compounds produced by HAB species commonly found in Florida (brevetoxins, okadaic acid, saxitoxins and Lyngbya toxins) in tissues and gut samples from manatee and green sea turtles that stranded in Florida, USA. Muscle, liver and alimentary tract samples were opportunistically collected from 14 manatees and 13 green turtles that stranded on the Florida shoreline between December 2003 and February 2006. Samples from each animal were assessed for the presence of brevetoxin, okadaic acid, lyngbyatoxin-A and saxitoxin. Nine (64%) manatees and 11 (85%) turtles were found to have been exposed to one or more of the HAB toxins. Okadaic acid and saxitoxin were only found in alimentary tract samples, whereas brevetoxin was more widely distributed. No lyngbyatoxin-A was observed in any tissue samples. The majority of turtles (13) stranded on the Atlantic coast between St. Johns and Monroe counties, with one on the Gulf coast at Bay County, whereas nine manatees were stranded on the Gulf coast between Levy and Lee counties, with the remaining five between Volusia and Brevard counties on the Atlantic coast. This HAB toxin screen has identified that a large proportion of a random sample of turtles and manatees that stranded in Florida in 2003–2006 were exposed to HAB toxins. Most of the concentrations measured were low, and the toxins were directly linked to the death of only three of these animals. However, the presence of these compounds, and in some cases the presence of multiple HAB toxins in individual animals, indicates that turtles and manatees in Florida are exposed to deleterious compounds at sub-lethal levels in their environment, which could ultimately compromise their health. 相似文献
14.
Yan Gao Ren-Cheng Yu Shauna A. Murray Jian-Hua Chen Zhen-Jun Kang Qing-Chun Zhang Fan-Zhou Kong Ming-Jiang Zhou 《Applied and environmental microbiology》2015,81(20):6973-6981
The identification of core genes involved in the biosynthesis of saxitoxin (STX) offers a great opportunity to detect toxic algae associated with paralytic shellfish toxins (PST). In the Yellow Sea (YS) in China, both toxic and nontoxic Alexandrium species are present, which makes it a difficult issue to specifically monitor PST-producing toxic algae. In this study, a quantitative PCR (qPCR) assay targeting sxtA4, a domain in the sxt gene cluster that encodes a unique enzyme involved in STX biosynthesis, was applied to analyze samples collected from the YS in spring of 2012. The abundance of two toxic species within the Alexandrium tamarense species complex, i.e., A. fundyense and A. pacificum, was also determined with TaqMan-based qPCR assays, and PSTs in net-concentrated phytoplankton samples were analyzed with high-performance liquid chromatography coupled with a fluorescence detector. It was found that the distribution of the sxtA4 gene in the YS was consistent with the toxic algae and PSTs, and the quantitation results of sxtA4 correlated well with the abundance of the two toxic species (r = 0.857). These results suggested that the two toxic species were major PST producers during the sampling season and that sxtA-based qPCR is a promising method to detect toxic algae associated with PSTs in the YS. The correlation between PST levels and sxtA-based qPCR results, however, was less significant (r = 0.552), implying that sxtA-based qPCR is not accurate enough to reflect the toxicity of PST-producing toxic algae. The combination of an sxtA-based qPCR assay and chemical means might be a promising method for monitoring toxic algal blooms. 相似文献
15.
16.
《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1999,721(1):47-54
The aim of the project was to develop a fast and reliable method for the quantification of the three tetracyclines: tetracycline, oxytetracycline and chlortetracycline in urine. The method is based on column-switching high-performance liquid chromatography with detection by MS–MS. Buffer is added to the sample before it is injected into the chromatographic system, and the first column which is an internal surface reversed-phase column separates the tetracyclines from the bulk of other compounds in urine. The tetracyclines are collected and concentrated on the analytical column before they are separated and eluted into the mass spectrometer in which the tetracycline are detected. The mass spectrometer is a triple quadrupole instrument and is equipped with an electrospray ion source. The MH+ ions are selected in the first quadrupole and collisionally activated in the collision cell. Upon collision, activation all three tetracyclines form fragment ions which could be assigned as: [M+H–H2O–NH3]+ which are selected in the sond mass filter. The detection limits for all three tetracyclines are about 10 ppb, and the calibration curves are linear from 10 to 1000 ppb. 相似文献
17.
Despite being an essential part of the marine food web during periods of ice cover, sea ice algae have not been studied in any detail in the Bering Sea. In this study, we investigated the diversity, abundance and ultimate fate of ice algae in the Bering Sea using sea ice, water and sub-ice sediment trap samples collected during two spring periods in 2008 and 2009: ice growth (March–mid-April) and ice melt (mid-April–May). The total ice algal species inventory included 68 species, dominated by typical Arctic ice algal diatom taxa. Only three species were determined from the water samples; we interpret the strong overlap in species as seeding of algal cells from the sea ice. Algal abundances in the ice exceeded 107 cells l?1 in the bottom 2-cm layer and were on average three orders of magnitude higher than in the water column. The vertical flux of algal cells beneath the ice during the period of ice melt (>108 cells m?2 day?1) exceeded export during the ice growth period by one order of magnitude; the vertical flux during both periods can only be sustained by the release of algae from the ice. Differences in the relative species proportions of algae among sample types indicated that the fate of the released ice algae was species specific, with some taxa contributing to seeding in the water column, while other taxa were preferentially exported. 相似文献
18.
Macroalgal-mediated transfers of water column nitrogen to intertidal sediments and salt marsh plants
In many temperate estuaries, mats of opportunistic macroalgae accumulate on intertidal flats and in lower elevations of salt marshes, perhaps playing a role in linking water column nitrogen (N) supply to these benthic habitats. Using a flow-through seawater system and tidal simulator, we varied densities (equivalent to 0, 1, 2, or 3 kg m−2 wet mass) of 15N-labelled macroalgae (Enteromorpha intestinalis) on estuarine sediments in microcosms with/without pickleweed (Salicornia virginica) to assess N transfers from algae. In the 6-week experiment, macroalgal biomass increased from initial levels in the lower density treatments but all algae lost N mass, probably through both leakage and decomposition. With all densities of algae added, sediments and pickleweed became enriched in 15N. With increasing mat density, losses of algal N mass increased, resulting in stepwise increases in 15N labeling of the deeper sediments and pickleweed. While we did not detect a growth response in pickleweed with macroalgal addition during the experiment, N losses from algal mats that persist over many months and/or recur each year could be important to the mineral nutrition of N-limited marsh plants. We conclude that N dynamics of intertidal sediments and lower salt marsh vegetation are linked to the N pools of co-occurring macroalgae and that further study is needed to assess the magnitude and importance of N transfers. 相似文献
19.
20.
The dumping of bauxite tailings on a clear-water Amazonian lake caused a significant decrease in phytoplankton densities. The influence of these suspended clay particles on algal sinking, through algae–clay aggregation, was investigated under laboratory conditions, by measuring fluctuations in algal population densities over time, with different suspended clay concentrations. The population densities of the four algal species tested, Phormidium amoenum, Mougeotia sp., Staurodesmus convergens and Chlorella sp., were decreased by the algae–clay aggregation. The extent of this process was dependent on algal morphological characteristics such as size and shape, as well as on the concentration of suspended clay particles. 相似文献