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Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene. 总被引:30,自引:11,他引:19
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J P Linton J Y Yen E Selby Z Chen J M Chinsky K Liu R E Kellems G F Crouse 《Molecular and cellular biology》1989,9(7):3058-3072
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The 5'-terminal ribonucleotide of an in vitro ColE1 transcript is not complementary to the DNA template 总被引:3,自引:0,他引:3
R C Parker 《Gene》1983,26(2-3):127-136
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Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum 总被引:15,自引:0,他引:15
A P Shuber E C Orr M A Recny P F Schendel H D May N L Schauer J G Ferry 《The Journal of biological chemistry》1986,261(28):12942-12947
The genes for the two subunits of the formate dehydrogenase from Methanobacterium formicicum were cloned and their sequences determined. When expressed in Escherichia coli, two proteins were produced which had the appropriate mobility on an SDS gel for the two subunits of formate dehydrogenase and cross-reacted with antibodies raised to purified formate dehydrogenase. The genes for the two formate dehydrogenase subunits overlap by 1 base pair and are preceded by DNA sequences similar to both eubacterial and archaebacterial promoters and ribosome-binding sites. The amino acid sequences deduced from the DNA sequence were analyzed, and the arrangement of putative iron-sulfur centers is discussed. 相似文献
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