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二次正交旋转回归设计在小菜蛾饲料配方筛选中的应用 总被引:1,自引:0,他引:1
应用二次正交旋转回归组合设计方法,筛选小菜蛾半合成人工饲料配方,以化蛹率为目标函数建立了二次回归模型,通过统计寻优获得的优化配方为:每100g人工饲料中,麦芽5g、蔗糖3.5g、菜叶粉5g、干酪素3g、螺旋藻0.75g。在温度24~26℃,相对湿度60%~70%,光照周期为13L∶11D的条件下,用该优化配方饲养小菜蛾,幼虫期8.45d,蛹期4.5d,初孵幼虫至成虫羽化的历期平均12.95d,化蛹率74.7%,羽化率94.9%,蛹重5.14mg/头,雌虫平均寿命9.5d,雄虫寿命13.5d,每雌产卵量135.67粒。与发芽菜苗饲养相比,存活率和发育速度无显著差异,蛹重和产卵量显著增加。该饲料价格低廉,配制和应用管理方便,饲养过程中不必更换饲料。 相似文献
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基于均匀设计与支持向量回归的棉铃虫幼虫全纯人工饲料配方优化 总被引:2,自引:0,他引:2
昆虫全纯人工饲料配方是精细研究昆虫营养生理的前提。本研究以棉铃虫Helicoverpa armigera幼虫为供试对象, 以本研究室前期发展的多因素多水平配方优化实验设计与分析方法UD-SVR为指导, 以略加修改的苹浅褐卷蛾Epiphyas postvittana幼虫全纯人工饲料为初始配方, 先优化20种天然氨基酸, 再优化蔗糖等其他8种非氨基酸营养组分, 经4轮97个处理组合, 获得一个棉铃虫幼虫全纯人工饲料满意配方, 平均蛹重、 化蛹率分别由初始配方的0.160 g和10.4%高效增至满意配方的0.255 g和97.9%。该全纯人工饲料满意配方的获得, 不但再次证实了UD-SVR方法的有效性, 同时为进一步深入研究棉铃虫营养需求与营养代谢奠定了基础。 相似文献
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为确定我国婴儿配方奶粉水溶性维生素B1、B2的科学设计范围,首先分析11家试验室对同批次产品的检测数值,确定维生素B1、B2用于配方设计的检测偏差均为15%,然后通过比较分析母乳数据、适宜摄入量和婴儿配方奶粉标准限量,确定维生素B1、B2设计的目标值分别为60、80 μg/100 kcal,最后根据已确定的营养素设计目标值,结合货架期衰减率、试验室间检测偏差推算合理的设计范围,并与可耐受最高摄入量、市售产品的营养含量分布作比较,同时兼顾现阶段0~6月龄婴儿的营养状况,确定我国婴儿配方奶粉维生素B1、B2的科学设计范围分别为86~180 μg/100 kcal、114~290 μg/100 kcal。 相似文献
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应用二次正交旋转组合设计对稻纵卷叶螟Cnaphalocrocis medinalis Guenée人工饲料的配方进行了优化,以化蛹率为目标函数建立了二次回归模型,结果表明稻叶粉、麦胚粉、玉米粉、干酪素、酵母对稻纵卷叶螟幼虫发育与化蛹率影响较大,大豆粉的影响不明显。通过统计寻优获得的优化配方为:每135 g人工饲料中,稻叶粉3 g、麦胚粉8 g、玉米粉4 g、干酪素4 g、酵母粉4 g。在温度27℃,相对湿度80%左右,光照L∶D=16∶8的条件下,使用该优化配方饲养稻纵卷叶螟,25.6%的供试初孵幼虫能完成幼虫发育并化蛹,幼虫期平均26.9 d,蛹重1622mg。与以前的研究结果比较,化蛹率与蛹重均有显著提高,而幼虫历期略短。 相似文献
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本研究旨在研制一种基于黄花菜的固体饮料。以黄花菜为主要原料,以感官评分、冲泡性能为指标,采用响应面试验对黄花菜固体饮料的配方和干燥工艺进行优化。试验表明,黄花菜固体饮料配方的响应面优化结果为:食盐添加量0. 01%,麦芽糊精添加量2. 0%,马铃薯全粉添加量1. 0%,奶粉添加量4. 5%,黄花菜与水比值1∶9(m∶v);此配方下研制出的黄花菜固体饮料流动性最佳,表征值为72. 855 mm。喷雾干燥的最佳工艺参数是进风温度为180℃,入料流量为1. 5 mL/min;所得样品的感官评分值为94. 2,流动性表征值为71. 65mm,润湿性表征值为27. 75 min。 相似文献
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改进支持向量机在棉铃虫人工饲料配方优化中的应用 总被引:2,自引:0,他引:2
发展新的实验设计与分析方法,通过实施尽可能少的实验而获得满意配方对动植物营养、发酵工程等复杂多因素多水平寻优问题极为重要。本研究结合均匀设计(uniform design, UD)与支持向量回归(support vector regression, SVR),提出了一种新的配方优化实验设计与分析方法UD-SVR,将其应用于棉铃虫Helicoverpa armigera (Hübner)幼虫人工饲料配方优化。结果表明:在考虑6因素时仅通过2轮22个实验,表征配方优劣的指标--平均蛹重即由初始的0.2436 g高效提升至0.3044 g,明显优于二次多项式偏最小二乘回归等经验风险最小参比模型。UD-SVR预测精度高、指导性强、可解释性好、优化高效,有望在多因素多水平配方优化中得到广泛应用。 相似文献
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木屑一直是灰树花主要的栽培基质,但木质资源日趋匮乏。本文采用单纯格子法筛选替代木屑基质的原材料及高产配方,以玉米芯、玉米秸秆、大豆秸秆、水稻秸秆为栽培基质,以产量为考核指标,建立各组分配比与产量之间的回归模型,优化栽培配方及考察配方中各组分的互作效应。结果表明:玉米芯可以替代木屑栽培灰树花,大豆秸秆、水稻秸秆和玉米秸秆可以与玉米芯进行合适配比,也可以替代木屑基质。优化的高产配方为75%玉米芯,23%麦麸,2%轻质碳酸钙,每袋产量平均达133.3 g,比对照配方平均产量(97.25 g)高出36.05 g,提高了37.07%。 相似文献
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Conidiobolus thromboides is an entomophthoralean fungus with potential as a biological control agent of aphids. However, its application in biological control is limited due to its formulation requirements. The objective of this study was to develop and optimise a novel air-extrusion method to embed C. thromboides hyphae at high density in alginate pellets. An orthogonal experimental design was used to investigate selected combinations of parameters known to affect hyphal density within pellets. The diameter of pellets produced, and the calculated density of hyphae within them, ranged from 0.18 ± 0.09 to 3.17 ± 0.06 mm and from 0.02 to 350.56 mg/mm3 respectively. These data were used to predict the optimal parameter combination to deliver the greatest density of hyphae of C. thromboides per pellet: 1% sodium alginate, a 1:2 ratio of hyphae to sodium alginate, an orifice diameter of 0.232 mm and an air pressure of 0.05 MPa. Pellets made under the optimal conditions predicted produced a mean total of 4.3 ± 0.6 × 105 conidia per pellet at 100% relative humidity which was significantly greater than the mean total number of conidia produced from infected aphid cadavers of comparable size (9.35 ± 0.85 × 104) (p < 0.001). In conclusion, air-extrusion embedding appears to be a promising method for formulating in vitro-produced hyphae of C. thromboides for use in biological control. 相似文献
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Application of statistically-based experimental designs for the optimization of nisin production from whey 总被引:4,自引:0,他引:4
Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett–Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l–1 for yeast extract, 0.569 g l–1 for KH2PO4, and 0.572 g l–1 for MgSO4. The validity of the optimal conditions was verified by a separate experiment. 相似文献
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癌症的基因组测序对于癌症的预防、诊断、预后、治疗以及基础生物学研究都有巨大的潜在的应用价值,正是由于其方向广泛,所以基因组测序的方案制定对于实现特定的研究目标,就显得尤为重要。同时,了解了基因组测序方案制定的规则,也有助于评估如今快速增长的发表文献的正确性和重要性。主要论述高通量测序技术在癌症基因组测序中的实际应用,并讨论癌症基因组测序在方案设计和方法学上如何调整,才能更好地实现特定的研究目的。 相似文献
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目的:探讨化瘀消癥剂口服、灌肠联合穴位离子导入多途径联合疗法治疗慢性盆腔痛(子宫内膜异位症、慢性盆腔炎性疾病)的临床疗效。方法:将60例慢性盆腔痛患者随机分为2组,每组28例。治疗组患者采用化瘀消癥剂口服、灌肠联合穴位离子导入方法;对照组患者采用散结镇痛胶囊进行口服,排除月经周期连续用药20天当做1个疗程,治疗持续3个疗程。治疗后,比较两组患者的总有效率、治疗前后中医症候以及局部体征评分、糖类抗原125(CA125)水平的变化。结果:治疗后,治疗组总有效率显著高于对照组(89.28%vs. 57.14%,P0.05)。两组患者的局部体征评分、中医症候评分和总积分均比治疗前有一定程度下降(P0.05)。两组患者治疗后的中医症候积分、总积分、体征积分均较治疗前明显降低,且治疗组以上指标均明显低于对照组(P0.05)。两种患者治疗后血清CA125水平均较治疗前明显降低(P0.05),但两组间比较差异无统计学意义(P0.05)。两组治疗期间不良反应的发生率对比差异无统计学意义(14.29%vs. 10.71%, P0.05)。结论:通过口服、灌肠外加穴位离子导入三种途径联合给予化瘀消癥剂治疗慢性盆腔痛患者可以显著提高其临床效果,有效改善患者的盆腔疼痛程度、中医症候和局部体征,安全有效,值得临床应用。 相似文献
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摘要 目的:探讨重建中气抗癌汤联合替吉奥抑制人胃癌SGC-7901细胞移植瘤生长的作用研究。方法:复制裸鼠皮下移植瘤模型,将裸鼠分为模型对照组、替吉奥组、联合1组、联合2组,并给药,比较各组裸鼠体重、瘤体积、瘤重及抑瘤率,Western Blot检测APC、ErbB1、MUC2、SIRT1蛋白表达水平。结果:与模型组比较,治疗组裸鼠体重出现不同程度下降(P<0.05),瘤体积均明显减小(P<0.05)、瘤重均明显减轻(P<0.05),APC表达逐渐升高(P<0.05)、ErbB1、SIRT1表达逐渐降低(P<0.05)、MUC2未见明显差异(P>0.05)。结论:重建中气抗癌汤联合替吉奥对胃癌荷瘤裸鼠肿瘤生长具有抑制作用,可能与上调APC表达、下调ErbB1、SIRT1表达有关。 相似文献
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Application of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis 总被引:2,自引:0,他引:2
Statistically based experimental designs were applied to the optimization of medium components and environmental factors for eicosapentaenoic acid (EPA) production by the diatom Nitzschia laevis in heterotrophic conditions. First, the Plackett-Burman design was used to evaluate the effects of variables including medium components and environmental factors on cell growth and EPA production. Among these variables, NaCl, CaCl(2), PI metal solution, pH, and temperature were identified to have the significant effects (with confidence level > 90 %). Subsequently, the concentrations of NaCl, CaCl(2), PI metal solution as well as the values of pH and temperature were optimized using central composite design. The cell growth and EPA production were found to respectively correlate to NaCl, CaCl(2), pH, and temperature that could be represented by second-order polynomial models. The optimal values of the four parameters were determined by response surface and numerical analyses as 8 g/L NaCl, 0.10 g/L CaCl(2), pH 8.5 and 19.8 degrees C for cell dry weight (DW), and 14 g/L NaCl, 0.10 g/L CaCl(2), pH 8.5 and 18 degrees C for EPA production, respectively. The subsequent verification experiments confirmed the validity of the models. This optimization strategy led to a DW of 9 g/L, an EPA yield of 280 mg/L and an EPA productivity of 28 mg/L/d, respectively, which were considerably higher than those obtained in the previous studies. 相似文献