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GAL4 activates gene expression in mammalian cells 总被引:73,自引:0,他引:73
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Mutants of GAL4 protein altered in an activation function 总被引:68,自引:0,他引:68
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GAL11P: a yeast mutation that potentiates the effect of weak GAL4-derived activators 总被引:24,自引:0,他引:24
A mutant yeast in which a weak GAL4-derived activator functions as a strong activator bears a single mis-sense mutation in GAL11 (a.k.a. SPT13). The first 74 amino acids of GAL4, including the zinc-dependent DNA binding region, attached to an acidic activating sequence, are sufficient to respond both to GAL11 and to our mutant GAL11P (potentiator). PPR1, a yeast activator with a similar zinc finger sequence, also responds to GAL11 and to GAL11P, whereas regulators bearing unrelated DNA binding motifs do not. GAL11 itself works as a strong activator when tethered to DNA by fusion to the bacterial LexA protein, and deletion of GAL11 is known to cause a 5- to 10-fold reduction in GAL4 activity. We suggest that a complex of GAL4 and GAL11 constitutes a particularly strong activator; evidence that the putative GAL4-GAL11 complex ordinarily forms preferentially on DNA suggests a biological rationale for GAL11 action. 相似文献
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Deletion analysis of GAL4 defines two transcriptional activating segments 总被引:179,自引:0,他引:179
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GAL4 protein: purification, association with GAL80 protein, and conserved domain structure. 总被引:16,自引:11,他引:5 下载免费PDF全文
Expression of the yeast Saccharomyces cerevisiae GAL4 protein under its own (galactose-inducible) control gave 5 to 10 times the level of protein observed when the GAL4 gene was on a high-copy plasmid. Purification of GAL4 by a procedure including affinity chromatography on a GAL4-binding DNA column yielded not only GAL4 but also a second protein, shown to be GAL80 by its reaction with an antipeptide antibody. Sequence comparisons of GAL4 and other members of a family of proteins sharing homologous cysteine finger motifs identified an additional region of homology in the middle of these proteins shown by genetic analysis to be important for GAL4 function. GAL4 could be cleaved proteolytically at the boundary of the conserved region, defining internal and carboxy-terminal folded domains. 相似文献
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An amino-terminal fragment of GAL4 binds DNA as a dimer 总被引:51,自引:0,他引:51
M Carey H Kakidani J Leatherwood F Mostashari M Ptashne 《Journal of molecular biology》1989,209(3):423-432