玉米和羽扇豆、鹰嘴豆间作对作物吸收积累Pb、Cd的影响

采用土壤盆栽试验研究玉米、羽扇豆和鹰嘴豆在不同分隔/间作方式下对Pb、Cd吸收积累的影响.试验结果表明,不同分隔/间作方式对玉米、羽扇豆和鹰嘴豆生物量变化有不同的影响.不同分隔/间作方式均显著影响玉米地下部对Pb的吸收,但是不同间作方式对玉米地上部Pb含量影响不大.塑料分隔以及玉米-鹰嘴豆间作可显著减少玉米地下部对Pb的吸收.在玉米单作中,塑料分隔和尼龙网分隔处理的玉米地下部Pb含量分别比不分隔处理时降低41.1%和33.3%.在不分隔处理时,玉米-鹰嘴豆间作的玉米地下部Pb含量仅分别为玉米单作和玉米-羽扇豆间作时的53.9%和63.8%.不同分隔方式对玉米地下部Cd含量影响较大,但是不管是间作还是分隔方式均对玉米地上部Cd含量影响不大.同时,还讨论了不同分隔/间作方式对羽扇豆和鹰嘴豆Pb、Cd含量的影响.作物根际土壤溶液Pb含量随着作物的生长而不断降低,但Cd含量却不断提高.不同分隔/间作方式对作物根际土壤溶液Pb、Cd含量也有一定影响.     

广西典型红壤旱地施用钙镁磷肥对玉米产量及其镉累积的影响

在广西典型类型红壤旱地布置玉米磷肥施用量的田间试验,研究不同钙镁磷肥施用量(磷肥Cd含量为0.0651 mg/kg)对玉米产量及地上部Cd累积的影响。结果表明,与不施磷肥处理(CK)相比,施磷肥可分别显著提高春、秋玉米籽粒产量8.2%—13.1%和13.7%—20.0%。高磷(600 kg P2O5/hm2)处理的春玉米秸秆产量比CK显著提高11.4%;施磷处理春、秋玉米秸秆Cd含量分别下降2.7%—45.8%和11.0%—43.6%;而籽粒Cd含量分别下降13.0%—40.6%和9.9%—31.5%,且秸秆和籽粒的Cd含量及累积量均随施磷量的增加而逐渐降低,其中以高磷处理最为显著。玉米秸秆及籽粒Cd累积量在高磷处理下(600 kg P2O5/hm2)分别比低磷处理(75—300 kg P2O5/hm2)降低13.6%—41.5%和8.8%—29.3%。相关分析表明,玉米Cd含量与土壤pH呈显著负相关,与土壤有效Cd含量呈显著正相关。施磷提高土壤pH,而降低土壤有效Cd含量。高量磷肥施用降低土壤Cd的有效性进而降低玉米对Cd的吸收累积。     

不同作物与玉米间作对玉米吸收积累镉的影响

采用盆栽技术,研究了7种间作作物对玉米吸收积累镉(Cd)的影响.结果表明,多数间作作物对玉米生物量没有明显影响,只有扁豆对玉米生长有较明显的抑制作用,使玉米生物量降至对照的67.8 %.间作豆科作物大幅提高玉米的Cd积累总量,其中鹰嘴豆的作用最为明显,使玉米对Cd的累积量增加1倍以上.7种间作植物对Cd也有不同水平的吸收,其中,油菜与籽粒苋可在地上部大量积累Cd（油菜地上部Cd浓度达53.9 mg·kg^-1,籽粒苋达51.0 mg·kg^-1),且籽粒苋根的Cd含量高达91.8 mg·kg^-1 (土壤Cd为3 mg·kg^-1).间作植物与玉米对Cd的吸收具有不同的交互作用特征,豆科作物在自身较少吸收Cd的条件下,显著提高了玉米对Cd的积累;籽粒苋在自身大量累积Cd的同时,一定程度上抑制了与其间作的玉米的Cd累积量;油菜地上部分吸收较多的Cd,但降低了玉米地上部分Cd含量.研究表明,如果将玉米作为大生物量的Cd污染修复植物来应用,可将其与豆科植物间作,以提高Cd清除效率;籽粒苋与油菜具有大量积累Cd的能力,可作为Cd污染土壤的修复植物.     

不同土壤水分条件下硅对紫花苜蓿水分利用效率及产量构成要素的影响

硅是地壳中含量仅次于氧的元素,植物不可能在无硅的环境中生长.通过盆栽试验研究了不同土壤水分条件下硅对紫花苜蓿(Medicago sativa)水分利用效率及产量构成要素的影响.结果表明,在土壤含水量为田间最大持水量的35%和80%的条件下,硅对紫花苜蓿水分利用效率和生物量没有显著影响,而在土壤含水量为田间最大持水量的50%和65%的条件下,硅显著提高了紫花苜蓿水分利用效率和生物量(p<0.05),紫花苜蓿水分利用效率的增幅分别为35%和20%,主要途径为降低叶片蒸腾速率;紫花苜蓿生物量增幅分别为41%和14%,主要通过促进分枝和株高生长,而不受单枝生物量的影响.因此硅对紫花苜蓿水分利用效率和生物量的有益作用与其生长环境中的土壤水分条件密切相关.     

两种药用植物生长和水分利用效率对干旱胁迫的响应

以田间持水量的30%、50%和75%为3个水分处理梯度,研究了决明子和菘蓝2种药用植物在不同水分处理和生长阶段的生长特性,并对稳定碳同位素分辨率(Δ¹³C)和水分利用效率(WUE)特征及二者之间的关系进行了分析．结果表明：与田间持水量的50%和30%相比,决明子和菘蓝在田间持水量75%的条件下具有较高的生长指标,说明这2种植物在充足或适当的水分条件下具有较高的生产力.随干旱胁迫程度的增加,决明子和菘蓝地上生物量和总生物量的Δ¹³C均逐渐减小,而地上生物量和总生物量的WUE均逐渐增加.决明子和菘蓝在不同水分处理之间各指标差异较小,说明这2种药用植物对干旱胁迫反应不敏感．菘蓝WUE与地上生物量和总生物量均呈显著负相关,决明子WUE与根冠比呈显著正相关.     

镉富集植物油菜与玉米间作对玉米吸收积累镉的影响

采用室内盆栽试验,在不同镉(Cd)添加水平下(0、2、5和10 mg·kg~(-1),分别记为Cd0、Cd2、Cd5和Cd10),研究玉米/油菜间作对营养生长期玉米生长和吸收积累Cd的影响。结果表明:间作促进了玉米生长,除Cd2处理外,间作玉米(Cd0、Cd5、Cd10)生物量比单作高19%~50%(P0.05);随着土壤中Cd浓度的增加,玉米各器官Cd含量也呈逐步增加的趋势,低、中浓度Cd污染条件下(Cd0、Cd2、Cd5),玉米/油菜间作可以阻控玉米地下部Cd向地上部转移,尤其是向叶转移,其中Cd2处理中间作玉米根系Cd含量与单作无显著差异,而间作玉米叶的Cd含量比单作低40%(P0.05);Cd5处理间作玉米根系Cd含量较单作显著增加25%(P0.05),而间作玉米叶的Cd含量较单作无显著增加;同处理间作玉米茎的Cd含量与单作间无显著差异;当土壤Cd污染浓度过高时,阻控作用会转变成促进效应,Cd10处理中间作玉米根系Cd含量与单作间无显著差异,而间作玉米叶、茎Cd含量比单作高17%、33%(P0.05);此外,土壤低、中浓度Cd污染条件下,间作对玉米单株Cd积累量的影响不明显,而高浓度Cd污染条件下(Cd10),间作显著提高玉米单株Cd积累量(61%)(P0.05)。因此,在低、中浓度Cd污染条件下,玉米/油菜间作对玉米吸收积累Cd有较明显的阻控效应,同时也是实现玉米安全、优质栽培的理想模式。     

干旱、CO2和温度升高对春小麦光合、蒸发蒸腾及水分利用效率的影响

研究了干旱、CO2 浓度和温度升高对春小麦生育期、光合速率 (Pn)、蒸发蒸腾 (ET)及水分利用效率 (WUE)的影响 .结果表明 ,大气CO2 浓度升高 (5 5 0、70 0 μmol·mol-1)虽可延长抽穗 成熟期 ,但高温 (日平均温度高于正常日平均温度约 4 .8℃ )对生育期的影响远大于高CO2 影响 ,使得高CO2 、高温下抽穗 成熟期缩短 ,且种子提前萌发 ;CO2 浓度升高和高温共同作用使各水分处理的小麦光合增强、气孔阻力增加、叶片水平的水分利用效率 (WUEl)和群体水平的水分利用效率 (WUE)增大 ,但对蒸腾速率影响不显著 .对蒸发蒸腾的影响因不同的土壤水分而不同 ,在高 (田间持水量的 75 %～ 85 % )、中 (田间持水量的 5 5 %～6 5 % )水分条件下 ,高温和高CO2 使蒸发蒸腾增加 ,而在低水分条件 (田间持水量的 35 %～ 4 5 % )下 ,高温和高CO2 使蒸发蒸腾减少     

伤根对谷子叶片光合速率及其产量的影响

利用谷子盆栽试验,研究了伤根措施对谷子叶片光合速率及其产量的影响,试验结果表明：1．苗期适度伤根能够提高叶片光合速率,而拔节期和孕穗期的处理则会降低叶片光合速率;2．伤根对谷子生物量的影响与土壤水分条件有关。在80％田间持水最条件下,谷子地上部生物最可提高1．5％～24．7％,而在50％田间持水量下,地上部生物量降低2．9％～19．0％。3．80％田间持水量时,随着伤根程度的加剧和伤根时间的后移,谷子籽粒产量下降;在50％田间持水量时,前期适度伤根处理有助于谷子籽粒产量的提高,而伤根量较为严重的处理和孕穗期伤根的处理都降低了谷子籽粒产量。     

土壤水分和氮添加对华北平原高产农田有机碳矿化的影响

通过105 d的恒温(25℃)控湿室内培养方法,探讨了华北平原高产粮田土壤有机碳矿化特征以及水分和有机、无机氮输入对其影响。试验设4个肥料添加水平和4个水分梯度,分别为对照(S0)、仅添加无机氮(尿素)(S1)、无机氮和有机氮(鸡粪)配施(S2)以及仅添加有机氮(S3)和25%(田间持水量;M0)、50%(M1)、75%(M2)和100%(M3)共16个处理,每处理3次重复。结果表明,各处理有机碳矿化速率均在培养后1 d达第1高峰,之后直线下降,培养7 d时下降幅度达57.2%—75.0%,培养20—30 d时出现第2高峰。有机碳累积矿化量有208.8—1161 mg/kg,主要集中在前30 d,可占整个培养期的59.1%—69.9%,105 d的净矿化率为0.07%—2.01%。根据双指数方程模拟结果,研究了土壤潜在矿化碳库(C1+C2),其中活性碳库(C1)和惰性碳库(C2)分别为53.0—135.1 mg/kg和156.9—1069 mg/kg,潜在矿化率为1.75%—9.66%。土壤含水量显著影响有机碳矿化,且与潜在矿化碳库呈二次函数关系(P0.05)。田间持水量25%—100%范围内,随着土壤含水量的升高,有机碳矿化速率呈增加趋势,但增幅降低,其中M2(田间持水量75%)的有机碳净矿化率最高。有机碳矿化量与土壤微生物碳和矿质氮含量呈线性正相关(P0.05),保持氮水平(200 kg N/hm2)相同,有机氮(鸡粪)和无机氮(尿素)均显著促进土壤有机碳矿化,但两者间差异不显著(P0.05),且有机氮和无机氮对有机碳矿化的影响均与土壤含水量有显著交互作用(P0.05)。     

土壤含水量对极小种群毛枝五针松幼苗抗氧化活性的影响

为探究水分对毛枝五针松(Pinus wangii)幼苗生理特性的影响,对不同土壤含水量下幼苗松针的抗氧化活性进行了研究。结果表明,土壤含水量为田间持水量的40%~80%时,其POD、SOD和CAT活性较强;随着处理时间的延长,细胞质膜渗透性减弱,抗逆性变弱;脯氨酸、可溶性糖和蛋白质含量均以20%~40%的田间持水量较高;处理30 d后丙二醛含量随着土壤含水量的增加而提高,在土壤含水量为田间持水量的40%时,丙二醛含量最低,抗逆性最强。因此,人工培育幼苗时,土壤水分过多的时间尽量不要超过30 d,控制土壤水分为田间最大持水量的30%~50%,这样能提高幼苗存活率。     

四种北方阔叶树种苗木对土壤镉胁迫的生长反应与抗性比较

采用盆栽方法,研究了4种北方阔叶树种茶条槭、山梨、五角槭和山荆子一年生苗木对土壤镉胁迫的生长反应和抗性.采用土壤和风化砂混合物作为栽培基质,设计5种土壤镉浓度梯度处理(对照、10、50、100、200mg·kg^-1).结果表明,低浓度处理(≤50mg·kg^-1)刺激各树种苗木的地径、树高生长,其中茶条槭在10mg·kg^-1处理下地径和苗高增加幅度最大,分别为对照的135.8%和146.0%;随着镉处理浓度的增加,地上部生长量下降,茶条槭在最高浓度处理下,生长量降低最大,地径和苗高仅分别为对照的241%和280%.叶片和新枝生物量变化存在较大种间差异,茶条槭和山梨在低浓度处理时总体上表现增加;山荆子新枝生物量增加,叶生物量减少;五角槭表现一致减少.最高浓度处理时,叶和新枝生物量受影响最大的是茶条槭,分别为对照的4.6%和7.3%;其次为五角槭,分别为对照的5.7%和7.9%;山梨和山荆子受影响的幅度相对较小.新根生物量除山梨外,其它3个树种苗木的生物量表现一致下降,其中下降幅度以茶条槭最大,最高浓度处理仅为对照的2.3%.山梨在低浓度处理下,新根生物量有所增加,分别为对照的125.5%和117.1%.     

德国鸢尾对Cd胁迫的生理生态响应及积累特性

通过盆栽研究了Cd胁迫下德国鸢尾的生长状况、生态效应、生理特性及吸收和富集Cd的能力.结果表明:德国鸢尾对小于5 mg/kg的Cd有较强的耐性,适用于城区土壤修复;Cd浓度大于5 mg/kg时抑制德国鸢尾生长,降低了其生态效应.随着Cd浓度的增大,德国鸢尾根系活力、叶绿素含量和含水量逐渐降低,游离脯氨酸和可溶性糖含量先升高后降低,细胞膜透性逐渐升高.Cd在德国鸢尾体内分布为根系＞地上部分,随着Cd浓度的增大,德国鸢尾根系和地上部分Cd积累浓度逐渐升高、富集系数和转运系数逐渐降低;Cd浓度为20 mg/kg时德国鸢尾对Cd的积累量最大,为2.122 mg/plant.     

Effects of cadmium and arsenic on growth and metal accumulation of Cd-hyperaccumulator Solanum nigrum L

Remediation of heavy metal contaminated sites using hyperaccumulators presents a promising alternative to current environmental methodologies. In the pot-culture experiment, the effects of Cd, and Cd-As on the growth and its accumulation in the Cd-hyperaccumulator (Solanum nigrum L.) were determined. No reduction in plant height and shoot dry biomass was noted when the plants were grown at Cd concentration of 1.0. The plant can be classified as a Cd-hyperaccumulator. Growing in the presence of 10 mg/kg Cd and 50 mg/kg As, the plant height and shoot dry matter yields did not decrease significantly (p>0.05) compared to that at 10 mg/kg Cd, however the stem Cd content increased by 28%. It was also observed that S. nigrum used exclusion strategy to reduce As uptake in the roots and restricted translocation into the shoots, resulting in As contents of the plant being root>leaf>stem>seed. The Cd accumulation capacity coupled with its relatively high As tolerance ability could make it useful for phytoremediation of sites co-contaminated by Cd and As.     

Assessing the potential for cadmium phytoremediation with Calamagrostis epigejos: a pot experiment

A pot experiment was conducted for three vegetation periods on a sandy soil (pH 7.5) to study the uptake and distribution of Cd in plant tissues of Calamagrostis epigejos (L.) Roth. Cadmium was applied as CdCl2 (a total of 11 solution of 0, 20. 100, and 200 mg Cd l(-1)). HNO3- and water-extractable concentrations of Cd in 2- and 20-cm soil depths were correlated with the applied Cd showing that Cd was very mobile in the soil. The uptake of Cd from soil by Calamagrostis epigejos was directly related to the total soil Cd content and to the water-soluble pool of Cd. The concentrations of Cd in plant tissues (roots, rhizomes, leaves) and litter increased with increased applied Cd. Most of the Cd that was taken up was accumulated in roots (range from 1.88+/-0.42 to 40.96+/-16.71 mg kg(-1) dry mass), followed by rhizomes (0.52+/-0.13 to 25.70+/-6.35 mg kg(-1)) and leaves (0.30+/-0.06 to 9.20+/-1.93 mg kg(-1)). Cd concentrations of the litter were about twofold greater than the concentrations in the leaves (0.67+/-0.07 to 18.98+/-7.00 mg kg(-1)). The bioaccumulation factor (leaf/soil concentration ratio) increased significantly from 0.70+/-0.10 (control) to 1.1+/-0.17 (100 mg Cd l(-1)), but decreased again at the highest Cd level (200 mg Cd l(-1)) toward 0.74+/-0.34, which was not significantly different from the control. The low transfer of Cd from soil to above-ground organs at higher soil Cd concentrations indicates an exclusion mechanism. The leaf/root Cd concentration ratio (translocation factor) shows no significant relationship to increasing soil contamination. Only 4-7% of the total plant Cd was accumulated in the above-ground tissues. The phytoextraction potential (total Cd removed from soil) within three growing seasons ranged from 0.11 to 0.25% of the total soil Cd. Total output in above-ground living and dead plant material of C. epigejos would be approximately 20 g ha(-1) a(-1) for the lowest contamination level (+20 mg Cd per pot) and approximately 275 g ha(-1) a(-1) for the highest contamination level (+200 mg Cd per pot). This is within the range where an application for phytoextraction of Cd has been suggested by other authors. However, we conclude that the practical use of C. epigejos for phytoremediation is not mainly in the field of phytoextraction, but phytostabilization. C. epigejos has the capability to structurally stabilize the soil and reduce Cd contamination spread due to erosion. The uptake of the available Cd pool and accumulation in below-ground biomass may further prevent leaching into ground water.     

岷江干旱河谷土壤水、氮和磷对小马鞍羊蹄甲幼苗生长的影响

资源阈值对植物的影响是恢复生态学的重要议题.通过近似模拟岷江干旱河谷自然干旱条件下水、氮和磷的阈值,设计析因实验,研究了一个生长季节内小马鞍羊蹄甲幼苗生长、生物生产量、资源利用效率和存活率的变化.结果表明：高水（40%田间持水量）、高磷（24 mg P·kg^-1）和低氮（100 mg N·kg^-1）处理分别促进了幼苗生长,增加了生物生产量,提高了存活率和水分利用效率,氮磷交互作用显著,水分和养分交互作用不明显.高氮（240 mg N·kg^-1）有强烈的负效应,高磷可以增大根面积、根长和根生物量,提高对氮和磷的吸收,缓解高氮的抑制作用.养分利用效率和幼苗根茎比呈显著正相关,并保持相对稳定.高水、高磷和低氮耦合有效地促进了幼苗的生长,而低水、低磷和高氮耦合则明显抑制了幼苗生长.     

Heavy Metals Bioremediation of Soil

Historical emissions of old nonferrous factories lead to large geographical areas of metals-contaminated sites. At least 50 sites in Europe are contaminated with metals like Zn, Cd, Cu, and Pb. Several methods, based on granular differentiation, were developed to reduce the metals content. However, the obtained cleaned soil is just sand. Methods based on chemical leaching or extraction or on electrochemistry do release a soil without any salts and with an increased bioavailability of the remaining metals content. In this review a method is presented for the treatment of sandy soil contaminated with heavy metals. The system is based on the metal solubilization on biocyrstallization capacity of Alcaligenes eutrophus CH34. The bacterium can solubilize the metals (or increase their bioavailability) via the production of siderophores and adsorb the metals in their biomass on metal-induced outer membrane proteins and by bioprecipitation. After the addition of CH34 to a soil slurry, the metals move toward the biomass. As the bacterium tends to float quite easily, the biomass is separated from the water via a flocculation process. The Cd concentration in sandy soils could be reduced from 21 mg Cd/kg to 3.3 mg Cd/kg. At the same time, Zn was reduced from 1070 mg Zn/kg to 172 mg Zn/kg. The lead concentration went down from 459 mg Pb/kg to 74 mg Pb/kg. With the aid of biosensors, a complete decrease in bioavailability of the metals was measured.     

The ability of Silybum marianum to phytoremediate cadmium and/or diesel oil from the soil

Phytoremediation is a new ecological and cost-effective technology applied for cleaning heavy metals and total petroleum hydrocarbon contaminated (TPH-contaminated) soils. This study was conducted to evaluate the potential of milk thistle (Silybum marianum) to phytoremediate cadmium (Cd (II)) from contaminated soils. To this end, the investigators applied a completely randomized design with the factorial arrangement and four replications. The results indicated that all the evaluated parameters of S. Marianum, including shoot and root fresh and dry weight, as well as shoot and root Cd, were significantly influenced by Cd (II) concentration and diesel oil (DO). The Cd-contaminated soil showed minor declining effects on the produced plant biomass, whereas the DO-contaminated soil had more inhibitory effects. Moreover, the soil contaminated with both Cd and DO led to adverse effects on the plant biomass. The shoot and root Cd concentration had an increasing trend in the presence of DO as the bioconcentration factor (BCF) by 1.740 (+90.78%), 1.410 (+36.89%), 2.050 (+31.41%), 1.68 (+32.28%), and 1.371 (+22.41%) compared to the soil without DO at Cd (II) concentrations of 20, 40, 60, 80, and 100 mg/kg, respectively. Biological accumulation coefficient also showed the same trend as the BCF. In all the treatments, the translocation factor was >1. Therefore, it was demonstrated that milk thistle had high potential for transferring Cd from root to shoot and reducing its concentration in the soil. Moreover, the study revealed that milk thistle had high potential for absorbing Cd in the soil contaminated with Cd and DO.     

N、P、K肥对香根草修复土壤镉、锌污染效率的影响

通过盆栽试验研究在30 mg/kg镉(Cd)污染土壤条件下N[CO(NH2)2:100、200、300 mg/kg土]、P(P2O5:50、100、200 mg/kg土)和K(KCl:100、200、300 mg/kg土)处理对香根草修复土壤Cd和锌(Zn)污染效率的影响。结果表明:3种N处理能促进香根草地上部生长,而且显著提高地上部特别是叶的Cd和Zn含量,导致其修复效率成倍显著增加;200 mg/kg K处理显著提高Zn修复效率,但300 mg/kg K和50、200 mg/kg P处理却显著降低Cd、Zn修复效率。因此,为改善香根草对较贫瘠土壤中Cd、Zn污染的修复效率,应对香根草适施N肥,并控制或者不施P、K肥为佳。     

土壤镉污染对四种阔叶树苗木叶绿素荧光特性和生长的影响

采用盆栽方法研究了土壤镉污染对白牛槭、刺玫果、金银忍冬和东北山梅花叶绿素荧光参数及生物量的影响.设置0、50、100和200 mg·kg^-1风干土4种镉浓度处理,测定苗木在不同镉浓度土壤中生长70 d后的叶绿素荧光参数.结果表明,随着镉处理浓度的提高,4种树种的F_v/F_m、ΦPSⅡ和qP均逐渐降低,qN则逐渐上升.4种苗木在一个生长季中的生物量累积随着镉浓度的增大逐渐降低.树种间荧光参数和生物量的变化幅度不同,白牛槭的变幅最大.刺玫果最小;以叶绿素荧光参数和生物量评价4树种抗镉污染能力,刺玫果最强、白牛槭最弱.     

Effects of biochar and nitrogen addition on nutrient and Cd uptake of Cichorium intybus grown in acidic soil

Biochar is an organic amendment used for soil remediation, there are only a few studies documenting the effects of nitrogen on the role of biochar in contaminated soils. A pot experiment was conducted to investigate the impacts of biochar (0%, 1%, and 2.5%, w/w) and nitrogen (0, 100, and 200 mg N kg^?1) on plant growth, nutrient and cadmium (Cd) uptake of Cichorium intybus. N, P, Ca, Mg, and Cd concentrations increased with N level in 0% and 1% biochar treatments. In plants treated with 2.5% biochar, 200 mg N kg^?1 addition caused significant reductions of N, P, Ca, Mg, and Cd concentrations in comparison to 100 mg N kg^?1 treatments. Nitrogen promoted shoot biomass at all biochar treatments, while biochar had no effect on shoot biomass in 0 and 200 mg N kg^?1 addition treatments. Nitrogen also significantly increased N, P, K, Ca, Mg, and Cd contents in the 0% and 1.5% biochar addition treatments. Although soil DTPA-extractable Cd concentration showed the lowest values in 1% biochar in combination with 100 and 200 mg N kg^?1 addition treatments, lowest shoot Cd concentration, and relatively high shoot biomass occurred in the 2.5% biochar + 200 mg N kg^?1 treatment. Based on these results, biochar application at its highest rate (2.5%) in combination with high N supply (200 mg N kg^?1) contributed to both crop yield and agricultural product safety. N input alone might increase the risk of human health, and the optimum N dose should be determined during phytostabilization process.