Brief Biography of Dr. Gui-fen ZHANG, Editor

1. Contact Information Department of Biological Invasion, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPP, CAAS), #12 South Street of Zhong-Guan-Cun, Beijing, 100081, China Phone: +86-10-82109572; Fax: +86-10-82105927 E-mail: guifenzhang3@163.com     

Brief Biography of Dr. Shu-sheng LIU, Deputy Editor-in-Chief

I. Contact Information Institute of Insect Sciences, Zhejiang University, 388 Yu Hang Tang Road, Hangzhou 310058, China Phone: +86-571-86971505 E-mail: shshliu@zju.edu.cn 2. Present Ranks Professor and Discipline Leader, Department of Plant Protection, Zhejiang University, China Lead Scientist, Innovative Research Group of Biological Contro     

A glimpse of life science research at Tsinghua

<正>Life Science at Tsinghua University has a long and eventful history.The Biology Department,founded in 1926,was among the oldest and most prestigious in China.In 1952,the Biology Department,together with other natural science departments,were eliminated from the University by order of the central government.In 1984,the Department of Biological Science and Biotechnology was re-established,with Professor Poo Mu-ming being the founding Director.In the ensuing 25 years,the Department went through rapid transformations and gained national and international recognition.In September 2009,the Department of Biological Science and Biotechnology was replaced by the School of Life Sciences.     

Identification of circulating CD90 CD73 cells in cirrhosis of liver

AIM:To identify circulating CD90 + CD73 + CD45 cells and evaluate their in vitro proliferating abilities.METHODS:Patients with cirrhosis(n=43),and healthy volunteers(n=40)were recruited to the study.Mononuclear cells were isolated and cultured from the peripheral blood of controls and cirrhosis patients.Fibroblast-like cells that appeared in cultures were analyzed for morphological features,enumerated by flow cytometry and confirmed by immunocytochemistry(ICC).Colony forming efficiency(CFE)of these cells was assessed and expressed as a percentage.RESULTS:In comparison to healthy volunteers,cells obtained from cirrhotic patients showed a significantincrease(P<0.001)in the percentage of CD90+CD73+ CD45 cells in culture.Cultured cells also showed 10 fold increases in CFE.Flow cytometry and ICC confirmed that the proliferating cells expressed CD90 + CD73 + in the cultures from cirrhosis patients.CONCLUSION:These results indicate the presence of circulating CD90 + CD73 + CD45 cells in patients with liver cirrhosis that have the potential to proliferate at a higher rate.     

Immunophenotyping of hematopoietic progenitor cells: Comparison between cord blood and adult mobilized blood grafts

AIM:To study the immunophenotype of hematopoietic progenitor cells from cord blood (CB) grafts (n = 39) in comparison with adult apheresis grafts (AG, n = 229) and pre-apheresis peripheral blood (PAPB) samples (n = 908) using flow cytometry analysis.METHODS: First, we performed a qualitative analysis of CD34+ cell sub-populations in both CB and PAPB grafts using the standardized ISHAGE protocol and a wide panel of 20 monoclonal antibodies. Next, we stud-ied some parameters, such as the age of mothers and the weight of newborns, which can influence the qual-ity and the quantity of CD34+ cells from CB. RESULTS: We found that the percentage of apoptotic cells was high in CB in comparison to PAPB (PAPB: 4.6% ± 2.6% vs CB: 53.4% ± 5.2%, P < 0.001). In CB, the weight of newborn and the age of the mother have the influence on CD34+ cells. The follow-up of Ag CD133in the ISHAGE double platform protocol in association with CD45, CD34 and the 7’AAD shows an equal rate between the two cell populations CD133+CD45+CD34+ high and CD34+CD45+ high with a higher percentage. So, is the inclusion of Ac CD133 necessary in the pres-ent panel included in the ISHAGE methodflLast part, we showed a signif icant presence of interferon γ in CB in comparison to PAPB, the annexin showing the high number of apoptotic cells in CB. CONCLUSION: This study demonstrates that many different obstetric factors must be taken into account when processing and cryo-banking umbilical CB units for transplantation.     

ON NEW SPECIES OF THE FAMILY THOMISIDAE FROM CHINA (ARACHNIDA: ARANEAE)

The present paper deals with nine species of spiders belonging to the. family .Thomisidae from China, types are deposited in the Institute of Zoology, Academia Sinica, Beijing and the Department of Biology, Bethune Medical University, Changchun, respectively. Diagnoses ofnew species are as follows:new speties are as follows:     

Immunophenotype and differentiation capacity of bone marrow-derived mesenchymal stem cells from CBA/Ca,ICR and Balb/c mice

AIM:To assess the capacity to isolate and expand mesenchymal stem cells(MSC)from bone marrow of CBA/Ca,ICR and Balb/c mice. METHODS:Bone marrow of tibia and femur were flushed,cultured and maintained in supplemented Dulbecco’s modified Eagle’s medium.MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106,Sca-1 and CD44 and negativity to CD45,CD11b and MHC classⅡ.Differentiation capacity of MSC towards osteogenic and adipo-genic lineages were also assessed. RESULTS:MSC were successfully cultured from bone marrow of all 3 strains,albeit differences in the temporal expression of certain surface antigens.Their differentiation into osteocytes and adipocytes were also observed. MSC from all 3 mouse strains demonstrated a shift from a haematopoietic phenotype(CD106-CD45+CD11b+Sca-1low)to typical MSC phenotype(CD106+CD45-CD11b-Sca-1high)with increasing passages. CONCLUSION:Information garnered assists us in the decision of selecting a mouse strain to generate MSC from for downstream experimentation.     

有关动物学国外学术会议消息(二)

1989年 (1)4月 (日期未定)第四届地中海甲壳动物——十足类专题学术讨论会,在希腊,塞萨洛尼基召开。会议由塞萨洛尼基大学生物学系主持。联系人及地址:Dr.Ath.Koukouras,Department of Zoology,University of Thessaloniki,54006 Thessaloniki,Greece. (2)4月1—3日 DNA复制和重组的分子机理学术讨论会,在美国,科罗拉多,开斯通召开。联系地址:UCLA Symposia,103 Molecular Biology Institute,University of California,Los Angelcs,CA90024-1378,USA。     

A New Isoflavonoid from Belamcanda chinensis （L.） DC.

A new isoflavonoid, 5, 6, 7, 3＇-terahydroxy-8, 4＇, 5＇-trimethoxyisoflavone （1）, along with 10 known isoflavonoids, namely 5, 6, 7, 4＇-tetrahydroxy-8-methoxyisoflavone （2）, irilone （3）, genistein （4）, tectorigenin （5）, irigenin （6）, irisflorentin （7）, dichotomitin （8）, dimethyltectorigenin （9）, iridin （10）, and tectoridin （11）, was isolated from the alcohol extract of the rhizomes of Belamcanda chinensis （L.） DC. The structures of these compounds were elucidated on the basis of results of spectroscopic analysis.     

邓叔群:菲茨帕特里克的弟子,分类学家之楷模(英文)

In 1946 I was a graduate student in Cornell University's Department of Plant Pathology, working on a thesis project, a worldwide monograph of a group of inoperculate discomycetes. My mentor, Professor Harry M. Fitzpatrick, pointed me to the works of Shu Ch黱 Teng, who twenty years earlier was also a graduate student in that Department. Luckily his book Higher Fungi of China (1939) as well as his papers into the 1940's appearing in Sinensia, and Mycologia, among others, were all in Engl…     

DU NOVAJ SPECIOJ DE ZICHYA BOLIVAR ELCINIO TWO NEW SPECIES OF ZICHYA BOLIVAR FROM CHINA(Orthoptera:Tettigoniidae)

This paper reports two new species of Zichya Bolivar from Gansu Prov-ince, China. All the type specimens are preserved in the Department of Bio-logy, Shaanxi Normal University.     

Novel pleiotropic loci controlling panicle architecture across environments in japonica rice （Oryza sativa L.）

To identify quantitative trait loci (QTLs) controlling panicle architecture in japonica rice, a genetic map was constructed based on simple sequence repeat (SSR) markers and 254 recombinant inbred lines (RILs) derived from a cross between cultivars Xiushui 79 and C Bao. Seven panicle traits were investigated under three environments. Single marker analysis indicated that a total of 27 SSR markers were highly associated with panicle traits in all the three environments. Percentage of phenotypic variation explained by single locus varied from 2% to 35%. Based on the mixed linear model, a total of 40 additive QTLs for seven panicle traits were detected by composite interval mapping, explaining 1.2%-35% of phenotypic variation. Among the 9 QTLs with more than 10% of explained phenotypic variation, two QTLs were for the number of primary branches per panicle (NPB), two for panicle length (PL), two for spikelet density (SD), one for the number of secondary branches per panicle (NSB), one for secondary branch distribution density (SBD), and one for the number of spikelets per panicle (NS), respectively. qPLSD-9-1 and qPLSD-9-2 were novel pleiotropic loci, showing effects on PL and SD simultaneously. qPLSD-9-1 explained 34.7% of the phenotypic variation for PL and 25.4% of the phenotypic variation for SD, respec- tively. qPLSD-9-2 explained 34.9% and 24.4% of the phenotypic variation for PL and SD, respectively. The C Bao alleles at the both QTLs showed positive effects on PL, and the Xiushui 79 alleles at the both QTLs showed positive effects on SD. Genetic variation of panicle traits are mainly attributed to additive effects. QTL × environment interactions were not significant for additive QTLs and additive × additive QTL pairs.     

美洲黑杨抗黑斑病基因的RAPD标记筛选和连锁分析

以美洲黑杨（Populus deltoides Bartr.cv.“Lux”(I-69/55)）为母本,欧美杨（P.euramericana cv.I-45）为父本得到的F1为材料,其中I-69对黑斑病表现为高度抗病,I-45为高度感病。利用分离群体混合分析（bulked segregrants analysis,BSA）技术建立两个DNA池（感病池和抗病池）,筛选出了与抗黑斑病性状基因相连锁的RAPD标记:OPAI17―1550、OPAI13―900。利用选择性基因型分析法进行标记―抗黑斑病基因连锁分析,两标记与抗黑斑病基因遗传距离分别为29.9cM和37.4cM。 Identification of Markers Linked to Resistance Locus of Marssonina Leaf Spot in Poplars by Bulked Segregant Analysis(BSA) ZHANG Bo1,HUANG Min-ren1,ZHUGE Qiang1,HAN Zheng-min1,YIN Tong-ming1,PAN Hui-xin1,ZHU Li-huang2,WU Rong-ling3,WANG ming-xiu１ 1.The Key Laboratory of Forest Tree Genetic Engineering,Nanjing Forestry University,Nanjing,210037,China; 2.The Institute of Genetics and Developmental Biology,Chinese Academy of Science,Beijing 100101,China; 3.Department of Statistics,University of Florida,Gainesville,Florida 32611,USA Abstract:DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA).The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv.“Lux”(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible).Out of 114 RAPD primers,four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus.The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively. Key words：BSA; RAPD; selective genotype analysis; Marssonina leaf spot     

Pollen Morphology of Parnassia L. （Parnassiaceae） and Its Systematic Implications

The pollen morphology of 28 species of Parnassia L. was investigated with light microscopy and scanning electron microscopy (SEM). The shape of pollen grains in this genus varies from subspheroidal to prolate in equatorial view and is three-lobed circular in the polar view. Pollen grains are usually radially symmetrical, isopolar, tricolporate or syntricolporate, with reticulate sculpture. The pollen characteristics among species are fairly similar to each other. Morphological information regarding the pollen grains shows that Parnassia is a natural genus. Based on exine ornamentation observed under SEM, three types of pollen grains were recognized: (i) type I, with foveolate-reticulate sculpture; (ii) type II, with a finely reticulate sculpture; and (iii) type III, with a coarsely reticulate sculpture. Most sections of this genus have one type of sculpture of pollen morphology, but Sect. Nectarotrilobos has three types of sculpture and Sect.Saxifragastrum has two types of sculpture. All three types of sculpture can be found in Southwest China,with species with the longest (Parnassia delavayi Franch.) and shortest (Parnassiafaberi Oliv.) colpi,implying that Southwest China is the center of diversification of the genus.     

A New Approach to Natural Products Discovery Exemplified by the Identification of Sulfated Nucleosides in Spider Venom

A New Approach to Natural Products Discovery Exemplified by the Identification of Sulfated Nucleosides in Spider Venom@Andrew E. Taggi$Contribution from The Cornell Institute for Research in Chemical Ecology and The Department of Chemistry and Chemical Biology, Baker Laboratory,Cornell UniVersity, Ithaca, New York 14853 @Jerrold Meinwald$Contribution from The Cornell Institute for Research in Chemical Ecology and The Department of Chemistry and Chemical Biology, Baker Lab…     

A novel system for Agrobacterium-mediated transformation of wheat（ Triticum aestivum L.） cells

A new approach for transforming the cultured cells of wheat (Triticum aestivum L.cv.Ganmai 8)was developed vsing Agrobacterium tumefaciens. The features of the optimum procedure were:(a)both combined synthetic signal molecules and multiple natural extracts from susceptible plants were used to pretreat the primary vigorous Agrobacterium(PVA)cells for approximately 16h:(b)the gyratory magnetic field condition was used during cocultivation;(c)the cocultivating period and selecting condition were modified;(d)the recipient cells were at exuberant metabolism and active division while infected with Agrobacterium.Both neomycin phosphotransferase and nopaline synthase assays demonstrated the expression of NPT Ⅱ and NOS genes.located on the T-DNA segment of chimaeric plasmid pGV3850::1103neo.in transformed wheat cell colonies by adopting the techniques of dot blot ndPAGE or high voltage paper electrophoresis,Integration of the foreign genes into wheat genome was confirmed by Southerm blot hybridization.Moreover.a relatively rational method was described for the estimation of transformation frequencies from cultured cell levels.     

A NEW SPECIES OF LITHODIDAE (CRUSTACEA, ANOMURA) FROM EAST CHINA SEA

In this paper, a new species of Lithodidae is reported. The type specimens are deposited in the Department of Biology, Hangzhou University and the Donghai Fisheries Research Institute. Diagnosis of the new species is given as below.