Objective: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk.
Methods: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants.
Results: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated.
Conclusions: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density. 相似文献
The soil utilized in this study was artificially contaminated by naphthalene and anthracene. The artificial contamination process was intended to simulate a recent accidental spillage of hydrocarbon fuels.
Several extractions, aimed at singling out the operating parameters (pressure, temperature, supercritical fluid mass flow rate) that are able to obtain the residual required concentration (50 mg/kg dry soil) in the shortest time, were carried out on a on-purpose made system.
The best extraction conditions were 120 bar and 40°C for a naphthalene contaminated soil and 200 bar and 80–100°C for an anthracene contaminated soil.
The results obtained in the experimental tests made it possible to build an analytical model able to correlate, for the given soil, the extraction length to the operating parameters such as supercritical fluid density, temperature and mass flow rate.
In order to evaluate the economic feasibility of the process, a unit treatment cost was evaluated for the case of an extraction carried out in a 10 m3 reactor in the presence of the best extraction conditions that were previously determined. The extraction unit cost was therefore equal to 35 000–65 000 €/t for a soil with a starting contaminant concentration equal to 1000 mg/kg of dry soil. 相似文献
Objective: The objective of this study is to explored novel biomarkers for phospholipidosis using a metabolomic approach.
Method: NMR spectrometry and LC/MS/MS analyses were applied to urine and plasma of rats administrated cationic amphiphilic drugs.
Results: The phenylacetylglycine to hippuric acid ratio in plasma was increased in time and dose-dependent manners; and it was well correlated with histopathological observation.
Conclusion: The plasma phenylacetylglycine to hippuric acid ratio is a potential marker in monitoring drug-induced phospholipidosis. 相似文献
Aims: Eggshell speckling in the Great Tit correlates with eggshell thickness and calcium availability. We investigated whether eggshell speckling changed in a Great Tit population during a period when soil calcium declined, and estimated changes in eggshell thickness.
Methods: Eggshell pigmentation of 2313 Great Tit clutches was recorded in 1988–2007 across a geological gradient in Wytham Woods, UK. Soil surveys conducted in 1974 and 1991 were repeated in 2008. Temporal and spatial changes in eggshell speckling and soil calcium were examined using generalized linear mixed models. We also estimated the change in eggshell thickness over this period.
Results: Soil-calcium availability declined 1974–2008 especially in calcium-rich limestone areas. A systematic change in eggshell speckling, approximating to a 6.5% reduction in eggshell thickness, occurred between 1988 and 2007. The change was greatest in parts of Wytham that experienced the greatest decline in soil calcium. Hatching success was unaffected.
Conclusion: Soil-calcium availability has declined in Wytham Woods, affecting Great Tit eggshell characteristics. This is the first evidence that declining soil calcium affects birds nesting in calcium-rich areas. Practical applications of these findings are considered. 相似文献
Objective: To establish a panel of protein biomarkers incorporated in a multiplexed microarray (BCa chip) and a classifier for diagnosing recurrent NMIBC.
Materials and methods: Urine samples from 45 patients were tested. Diagnostic performance was evaluated by receiver operating characteristic (ROC) analysis.
Results: A multi biomarker panel (ECadh, IL8, MMP9, EN2, VEGF, past recurrences, BCG therapies and stage at diagnosis) was identified yielding an area under the curve of 0.96.
Discussion and conclusion: This biomarker panel represents a potential diagnostic tool for noninvasive diagnosis of recurrent NMIBC. 相似文献
Objective: To examine effects of nickel exposure on plasma oxidative and anti-oxidative biomarkers.
Materials and methods: Biomarker data were collected from 154 workers with various levels of nickel exposure and from 73 controls. Correlations between nickel exposure and oxidative and anti-oxidative biomarkers were determined using linear regression models.
Results: Workers with a exposure to high nickel levels had significantly lower levels of anti-oxidants (glutathione and catalase) than those with a lower exposure to nickel; however, only glutathione showed an independent association after multivariable adjustment.
Discussion and conclusion: Exposure to high levels of nickel may reduce serum anti-oxidative capacity. 相似文献
Methods: Secretome and miRNAs were analyzed during the first two weeks and on clinical suspicion of ACR in the bile of 45 OLT recipients.
Results: Levels of CD44, CXCL9, miR-122, miR-133a, miR-148a and miR-194 were significantly higher in bile of patients who developed ACR within the first 6 months after OLT and during ACR.
Conclusion: Analysis of secretome and miRNA in bile could improve our understanding of the local inflammatory process during rejection. 相似文献
Materials and methods: The association between serum YKL-40 and predefined outcome was evaluated in 1344 HF patients assigned to rosuvastatin or placebo.
Results: YKL-40 was not associated with outcome in adjusted analysis. In YKL-40 tertile 1, an effect on the primary outcome (HR 0.50, p?=?0.006) and CV death (HR 0.54, p?=?0.040) was seen by rosuvastatin in adjusted analysis.
Conclusions: A beneficial modification of outcome was observed with statin therapy in patients with low YKL-40 levels. 相似文献
Objective: To assess oral riboflavin is an adherence marker.
Methods: Riboflavin was incorporated into active treatment and placebo pills for a clinical trial lasting for 2 years.
Results: The accuracy (area under the receiver operating curve) of urinary riboflavin was 0.91 as a binary classifier of adherence, and was similar or better than for two active study ingredients daidzein (0.92) and genistein (0.87) (all p?<?0.0001). Decreased adherence over time was similar in the two study groups.
Conclusion: Riboflavin is an accurate and useful biomarker for study pill ingestion. 相似文献
Objective: To evaluate circulatory apoptotic markers and oxidative stress in patients with PCOS.
Materials and methods: Forty-four women with PCOS, and 44 healthy women as controls were enrolled in the study. Oxidative stress parameters and caspases levels were measured in serum.
Results: The caspase 9 level was significantly lower and related with oxidant status in patients with PCOS, while the circulating levels of caspases 3 and 7 were statistically similar in both groups.
Discussion: This study is the first report demonstrating the circulating levels of apoptotic markers and their relationship with oxidant status in PCOS.
Conclusion: The circulating caspase 9 and oxidant status might contribute to apoptotic dysregulation in PCOS. 相似文献
Objective: The objective of this study is to investigate the association between sleep-disordered breathing (SDB) – a source of oxidative stress – and CML.
Materials and methods: About 1002 participants in the Cardiovascular Health Study (CHS) were studied.
Results: Women with SDB had significantly higher CML concentration compared with those without SDB (OR?=?1.63, 95%CI?=?1.03–2.58, p?=?0.04). The association was not significant among men.
Discussion: SDB was associated with CML concentration among elderly women but not men in the Cardiovascular Health Study.
Conclusion: Accumulation of CML may be an adverse health consequence of SDB 相似文献
Objective: This review aimed to highlight biomarker characterisation and assessment of unique bacterial pili.
Methods: A PubMed search for bacterial pili, diagnostics, vaccine and therapeutics was performed, with emphasis on the well characterised pili.
Results: In total, 46 papers were identified and reviewed.
Conclusion: Extensive analyses of pili enabled by advanced nanotechnology and whole genome sequencing provide evidence that they are strong biomarker candidates. Mycobacterium tuberculosis curli pili are emphasised as important epitopes for the development of much needed point-of-care diagnostics and therapeutics. 相似文献
Objective: Evaluation of micronuclei (MN) as a screening marker of occupational ionizing radiation (IR) exposure.
Materials and methods: Using micronucleus test, peripheral blood lymphocytes (PBL) of 402 control and exposed subjects were screened for genetic damage.
Results: The mean frequencies of micronucleus test parameters were significantly higher in exposed persons. Increase of micronucleus yield with duration of exposure (DOE) by 0.303MN/year was revealed.
Discussion and conclusion: The obtained data encourage us to consider MN as valuable markers for preventive medical screening of occupationally exposed groups. 相似文献
PFBSK did not show acute and chronic aquatic, microorganisms and terrestrial animal toxicity, while exhibited some terrestrial plants toxicity. The ecotoxicology of PFBSK decreased sharply except for terrestrial plants compared with PFOS. Especially, for acute and chronic aquatic ecotoxicity, PFBSK is lower than 100 times those of PFOS.
Although the similar terrestrial plants toxicity level was observed for PFBSK and PFOS, the lower risk of PFBSK was deduced for the reason that there was far less chances to be exposed and retained in the soil and sediment for its high water solubility and low adsorption.
In conclusion, PFBSK showed lower ecotoxicity hazard and risk than those of PFOS. PFBSK could be an environmental friendly alternative to PFOS. 相似文献
The methodology of recording and systematization includes the following parameters: coal deposits; climate parameters; roads; rivers; land and surrounding objects; air polluters; water polluters; and soil polluters. Methods for calculating land deformation, air polluter emissions, and noise impact are also presented.
Based on the number and specificity of analyzed data, the paper provides a concept of the IS and an overview of environmental impact of underground coal mine technological units. The concept was used to present the results of a research conducted at the underground coal mine “Soko” in Serbia.
The results of this research can help many potential users realize their goals. Those goals are preventive by nature, since negative environmental impact can be predicted, which enables the environmental protection experts to take appropriate measures. 相似文献
Objectives: We have examined the incremental value of Big-ET-1 in predicting total and CV mortality next to the well-established CV risk marker N-Terminal Pro-B-Type Natriuretic Peptide (NT-proBNP).
Methods: Big-ET-1 and NT-proBNP were determined in 2829 participants referred for coronary angiography (follow-up 9.9 years).
Results: Big-ET-1 is an independent predictor of total, CV mortality and death due to CHF.
Discussion: The conjunct use of Big-ET-1 and NT-proBNP improves the risk stratification of patients with intermediate to high risk of CV death and CHF.
Conclusions: Big-ET-1improves risk stratification in patients referred for coronary angiography. 相似文献
Aims: To produce estimates of the size of the Ring Ouzel breeding population in the UK and its constituent countries.
Methods: A stratified random selection of tetrads was surveyed within the known breeding range, except for the remaining breeding areas on Dartmoor and historical sites elsewhere, which were surveyed in their entirety. The methods followed those devised for the first UK-wide survey in 1999, using playback of song at specific points along tetrad transect lines.
Results: The UK population estimate in 2012 was 5332 (95% confidence limits (CL), 4096–6875) territories, a non-significant decline of 29% since 1999. There were no significant differences in the population changes in England, Scotland and Wales between the two surveys. An estimation of survey efficiency found that 84% of territories were located by the national survey methods, suggesting that the UK population in 2012 could have been as many as 6348 (95% CL, 4825–8198) territories.
Conclusion: The results suggest that the UK Ring Ouzel population has continued to decline since 1999. Ongoing and future research should determine where the main threats to the population are: on the breeding or wintering grounds, or during migration. 相似文献
Objectives: Few studies have compared the expression of inflammatory or adhesion molecules between coronary artery disease (CAD) versus CSX.
Materials and methods: Ninety-two CSX and 145 CAD subjects without known diabetes mellitus underwent coronary angiogram for angina.
Results: Vascular cell adhesion molecule (VCAM)-1 (median, 507 versus 431?ng/ml, p?=?0.001) was significantly higher in the CAD group. In the binary regression, VCAM-1 was a significant differential factor for CAD versus CSX.
Discussion and conclusion: Adhesion molecules might be implicated in the differential expression of macro versus microvascular coronary disease.
Trial registration number: NCT01198730 at https://clinicaltrials.gov 相似文献
Objective: To evaluate the risk of pneumonia-induced sepsis by examining its linkage with polymorphisms of IL-6 and IL-10.
Materials and methods: Samples were obtained from 188 pneumonia-induced sepsis patients, 162 pneumonia patients and 200 healthy controls.
Results: Subjects with IL-10 -1082 AA genotypes and IL-6 -174?CC genotype had a higher risk of sepsis and increased mRNA levels.
Conclusion: The variants of IL-10 -1082 A allele and IL-6 -174 C allele contributed to an increased risk of pneumonia-induced sepsis. 相似文献
Objective: To evaluate the relationship between sCD40l and survival, CVE and mortality in HD patients on long-term follow-up.
Methods: We registered 46?HD patients’ baseline characteristics, mortality and CVE for 108 months.
Results: SCD40l correlated positively with C-reactive protein, was higher in survivors, but had no impact on survival and was not predictive for CVE or CV mortality.
Conclusion: The levels of sCD40l have no influence on survival or CVE and mortality in HD patients in a long-term follow-up. 相似文献