Pavement Life Cycle Assessment Workshop,May 5–7, 2010 in Davis,California, USA

Purpose  

A workshop was convened on life cycle assessment (LCA) applied to pavement. The workshop’s primary goals were to establish common practices for conducting LCAs for pavements. In general, pavement LCA has been implemented without clear guidelines for modeling assumptions and reporting. This shortcoming has led to challenges in interpreting and comparing pavement LCA outcomes.     

Changes in Water Uptake in Light and Darkness of the Barley Cultivar ‘Professor Schiemann’ Infected with Powdery Mildew

In the investigated barley cultivar resistant toErysiphe graminis f. sp.hordei Marchal a temporary decrease in water uptake may be observed during the first days following infection in light. No marked differences could be detected in darkness. Starting from the stage of formation of fructification organs the ratio between water uptake in light and in darkness in the resistant cultivar is similar to that of the control, while the opposite is true in the susceptible cultivar where the ratio markedly decreases with the development of powdery mildew. The resistant cultivar responds more rapidly to the pathogen by changes in water uptake than the susceptible one.     

Natural Selection Causes Adaptive Genetic Resistance in Wild Emmer Wheat against Powdery Mildew at “Evolution Canyon” Microsite,Mt. Carmel,Israel

Background

“Evolution Canyon” (ECI) at Lower Nahal Oren, Mount Carmel, Israel, is an optimal natural microscale model for unraveling evolution in action highlighting the basic evolutionary processes of adaptation and speciation. A major model organism in ECI is wild emmer, Triticum dicoccoides, the progenitor of cultivated wheat, which displays dramatic interslope adaptive and speciational divergence on the tropical-xeric “African” slope (AS) and the temperate-mesic “European” slope (ES), separated on average by 250 m.

Methods

We examined 278 single sequence repeats (SSRs) and the phenotype diversity of the resistance to powdery mildew between the opposite slopes. Furthermore, 18 phenotypes on the AS and 20 phenotypes on the ES, were inoculated by both Bgt E09 and a mixture of powdery mildew races.

Results

In the experiment of genetic diversity, very little polymorphism was identified intra-slope in the accessions from both the AS or ES. By contrast, 148 pairs of SSR primers (53.23%) amplified polymorphic products between the phenotypes of AS and ES. There are some differences between the two wild emmer wheat genomes and the inter-slope SSR polymorphic products between genome A and B. Interestingly, all wild emmer types growing on the south-facing slope (SFS=AS) were susceptible to a composite of Blumeria graminis, while the ones growing on the north-facing slope (NFS=ES) were highly resistant to Blumeria graminis at both seedling and adult stages.

Conclusion/Significance

Remarkable inter-slope evolutionary divergent processes occur in wild emmer wheat, T. dicoccoides at EC I, despite the shot average distance of 250 meters. The AS, a dry and hot slope, did not develop resistance to powdery mildew, whereas the ES, a cool and humid slope, did develop resistance since the disease stress was strong there. This is a remarkable demonstration in host-pathogen interaction on how resistance develops when stress causes an adaptive result at a micro-scale distance.     

Isolation and Characterization of a Barley Chitinase Genomic Clone—Expression in Powdery Mildew Infected Barley

Chitinases (E.C.3.2.1.14) are thought to play an important role in the defense of plants against fungal invasion. By screening a barley genomic library with a previously identified chitinase eDNA clone (clone 10), a genomic clone was isolated and characterized by DNA sequencing of the chitinase coding region and flanking sequences. This clone contains an open reading frame capable of coding for a 34 kD chitinase. Comparison of the amino acid sequence of the encoded protein with other barley chitinases suggests that the genomic clone encodes chitinase T, which has been characterized extensively by protein sequencing. Treatment of barley leaves and aleurone protoplasts with N-acetyl glucosamine oligomers which act as elicitors in other plants, did not lead to the elevation of the levels of the chitinases. However, infection of barley seedlings with the powdery mildew fungus, Erysiphe graminis, resulted in the induction of several isoforms of chitinase. The level and number of chitinase isozymes was correlated with the severity of infection. The infection-related chitinases found in barley leaves are different from those found in seeds.     

Molecular Farming – OECD Workshop

Transgenic Research -     

A Brief Report on the Seventh International Workshop on Seed Biology, Salamanca, Spain, May 2002

TheSeventhInternationalWorkshoponSeedBiolo gywasheldfrom 1 2to 1 6May ,2 0 0 2inUniversityofSalamanca ,Spain .ThiswasthefirstworkshoptobeheldentirelyundertheInternationalSocietyforSeedScience(ISSS)banner.About 30 0seedscientistsfrom 2 0coun triesattendedthisworkshop .Aftertheopeningceremony ,thepresident_elect,Prof.DerekBewley ,fromGuelphUniversityofCanada ,openedwithastimulatingoverviewofseedsinscienceandsociety ,whichwasentitled“SeedsofHope,SeedsofDespair” .Hereviewedthehis tory…     

Morphological Variability of Wheat Powdery Mildew in the Context of Its Parasitic Adaptation to Wheat–Aegilops Lines with Different Resistance

The interaction between the pathogen and wheat–Aegilops lines with different resistance as well as their parental forms in the course of powdery mildew infection was studied using scanning electron microscopy. The course of infection in the line 51/99i and its parental form, the Rodina variety, proved to be similar. The plants of both genotypes featured pronounced adhesion of the primary infection structures to the surface of plant epidermal cells and the formation of large, well-developed colonies of the fungus, which was evidence for parasite–host compatibility. The development of powdery mildew on the line 135/99i was similar to that on the parental form Aegilops speltoides K-389. The pathogen–host interaction was characterized by a longer incubation period. Sparse fungal colonies were formed from mycelial hyphae with multiple hyphal lobes, and their adhesion to the surface of the epidermal cells was disturbed in most cases. Such a pattern of pathogen development indicated that the host plants had some resistance factors operating mainly at the level of pathogen penetration. The types of resistance in lines 95/99i and 56/99i were not characteristic of the parental form Ae. speltoides K-389, but they were described for Ae. speltoides samples from other natural ranges (Ryabchenko et al., 2002). This fact suggests that the immune potential of Ae. speltoides as a species is polygenic, and its elements can be transmitted to hybrids irrespective of concrete plants used as the donors of resistance.