共查询到20条相似文献,搜索用时 0 毫秒
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Thorsen K Sørensen KD Brems-Eskildsen AS Modin C Gaustadnes M Hein AM Kruhøffer M Laurberg S Borre M Wang K Brunak S Krainer AR Tørring N Dyrskjøt L Andersen CL Orntoft TF 《Molecular & cellular proteomics : MCP》2008,7(7):1214-1224
Alternative splicing enhances proteome diversity and modulates cancer-associated proteins. To identify tissue- and tumor-specific alternative splicing, we used the GeneChip Human Exon 1.0 ST Array to measure whole-genome exon expression in 102 normal and cancer tissue samples of different stages from colon, urinary bladder, and prostate. We identified 2069 candidate alternative splicing events between normal tissue samples from colon, bladder, and prostate and selected 15 splicing events for RT-PCR validation, 10 of which were successfully validated by RT-PCR and sequencing. Furthermore 23, 19, and 18 candidate tumor-specific splicing alterations in colon, bladder, and prostate, respectively, were selected for RT-PCR validation on an independent set of 81 normal and tumor tissue samples. In total, seven genes with tumor-specific splice variants were identified (ACTN1, CALD1, COL6A3, LRRFIP2, PIK4CB, TPM1, and VCL). The validated tumor-specific splicing alterations were highly consistent, enabling clear separation of normal and cancer samples and in some cases even of different tumor stages. A subset of the tumor-specific splicing alterations (ACTN1, CALD1, and VCL) was found in all three organs and may represent general cancer-related splicing events. In silico protein predictions suggest that the identified cancer-specific splice variants encode proteins with potentially altered functions, indicating that they may be involved in pathogenesis and hence represent novel therapeutic targets. In conclusion, we identified and validated alternative splicing between normal tissue samples from colon, bladder, and prostate in addition to cancer-specific splicing events in colon, bladder, and prostate cancer that may have diagnostic and prognostic implications. 相似文献
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Regulating retrotransposon activity through the use of alternative transcription start sites 下载免费PDF全文
Jenna Persson Babett Steglich Agata Smialowska Mette Boyd Jette Bornholdt Robin Andersson Catherine Schurra Benoit Arcangioli Albin Sandelin Olaf Nielsen Karl Ekwall 《EMBO reports》2016,17(5):753-768
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Improved prediction of bacterial transcription start sites 总被引:2,自引:0,他引:2
Gordon JJ Towsey MW Hogan JM Mathews SA Timms P 《Bioinformatics (Oxford, England)》2006,22(2):142-148
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Multiple transcription start sites and alternative splicing in the methylenetetrahydrofolate reductase gene result in two enzyme isoforms 总被引:4,自引:0,他引:4
Pamela Tran Daniel Leclerc Manuel Chan Aditya Pai Francois Hiou-Tim Qing Wu Philippe Goyette Carmen Artigas Renate Milos Rima Rozen 《Mammalian genome》2002,13(9):483-492
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