共查询到20条相似文献,搜索用时 406 毫秒
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Tone M Powell MJ Tone Y Thompson SA Waldmann H 《Journal of immunology (Baltimore, Md. : 1950)》2000,165(1):286-291
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Izumi M Yokoi M Nishikawa NS Miyazawa H Sugino A Yamagishi M Yamaguchi M Matsukage A Yatagai F Hanaoka F 《Biochimica et biophysica acta》2000,1492(2-3):341-352
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Mouse integrin alphav promoter is regulated by transcriptional factors Ets and Sp1 in melanoma cells
A 17-bp region between the -31 and -15 bp region of the mouse integrin alphav gene is known to be one of the cis-acting elements for promoter activity. Experimental binding of nuclear proteins to the -31/-15 region reveals that the -27/-16 region mediates the binding. The -27/-16 region, GGCTCCTCCTCC, has a TCCTCC motif, one of the Sp1 binding motifs. An anti-Sp1 IgG and an Sp1-binding oligonucleotide interfered with the binding of nuclear proteins to the -27/-16 oligonucleotide, demonstrating that Sp1 binds to the -27/-16 region. In addition to the -27/-16 region, two other regions, -108/-89 and -64/-44, were found to bind to nuclear proteins within the -108/+1 alphav promoter region. An oligonucleotide containing the Ets-binding consensus sequence of CAGGAAGT interfered with their binding, indicating that both regions have a functional Ets-binding site; which is ACGGAAGT from -106 to -99 bp and ACTTCCTC from -61 to -54 bp, as deduced from the sequence. Mutations in or deletions from any one of three cis-acting elements, the two Ets-binding sites or one Sp1-binding site, remarkably decreased the promoter activity detected in the -108/+1 region. Cotransfection of both Sp1 and Ets-1 cDNAs with the -108/+1 region into B16F10 cells increased the promoter activity 2.9-fold. These results demonstrate that Sp1 and Ets cooperate to activate the -108/+1-alphav promoter region. 相似文献
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Involvement of nuclear transcription factor Sp1 in regulating glucose transporter-1 gene expression during rat trophoblast differentiation. 总被引:3,自引:0,他引:3
Y Okamoto M Sakata T Yamamoto Y Nishio K Adachi K Ogura M Yamaguchi T Takeda K Tasaka Y Murata 《Biochemical and biophysical research communications》2001,288(4):940-948