蝮蛇类凝血酶基因的分析及表达研究

从蝮蛇（ＡｇｋｉｓｔｒｏｄｏｎｈａｌｙｓＰａｌａｓ）毒腺中抽提总ＲＮＡ,经ＲＴＰＣＲ扩增该基因,克隆后经全序列测定,蝮蛇类凝血酶ｐａｌａｓｅ的ｃＤＮＡ长７０８个核苷酸,即编码２３６个氨基酸;根据同源性,推测该类凝血酶ｐａｌａｓｅ的活性中心为Ｈｉｓ４１、Ａｓｐ８６和Ｓｅｒ１８２;二硫键为Ｃｙｓ７Ｃｙｓ１３９、Ｃｙｓ２６Ｃｙｓ４２、Ｃｙｓ７４Ｃｙｓ２３４、Ｃｙｓ１１８Ｃｙｓ１８８、Ｃｙｓ１５０Ｃｙｓ１６７和Ｃｙｓ１７８Ｃｙｓ２０３;该蝮蛇毒类凝血酶ｃＤＮＡ序列及推导的氨基酸序列均为首次报道。构建Ｔ７启动子控制下的ｐａｌａｓｅ的大肠杆菌表达质粒,ＩＰＴＧ诱导ｐａｌａｓｅ获得表达。     

川东北二叠纪吴家坪期牙形石（刺）序列及其世界对比

对四川省宣汉县渡口和南江县桥亭吴家坪期地层的系统采集和研究结果表明,吴家坪期至少包括７个牙形石带,自下而上为Ｃｌａｒｋｉｎａｄｕｋｏｕｅｎｓｉｓ带,Ｃ．ａｓｙｍｍｅｔｒｉｃａ带,Ｃ．ｌｅｖｅｎｉ带,Ｃ．ｇｕａｎｙｕａｎｅｎｓｉｓ带,Ｃ．ｔｒａｎｓｃａｕｃａｓｉｃａ带,Ｃ．ｏｒｉｅｎｔａｌｉｓ和Ｃ．ｉｎｆｌｅｃｔａ带。其中,前３个带的层位低于一直作为吴家坪期早期的Ｃ．ｌｉａｎｇｓｈａｎｅｎｓｉｓ带。吴家坪阶的顶界以置于Ｃ．ｉｎｆｌｅｃｔａ带与Ｃ．ｓｕｂｃａｒｉｎａｔａ带之间较妥。同时,建立了Ｃｌａｒｋｉｎａｄｕｋｏｕｅｎｓｉｓ,Ｃ．ａｓｙｍｍｅｔｒｉｃａ,Ｃ．ｂｉｚａｒｒｅｎｓｉｓ,Ｃ．ｄａｘｉａｎｅｎｓｉｓ,Ｃ．ｄｅｍｉｃｏｒｎｉｓ,Ｃ．ｉｎｆｌｅｃｔａ,Ｃ．ｌｏｎｇｉｃｕｓｐｉｄａｔａ等７个新种。     

云南吕合地区晚晚第三纪孢粉植物群

吕合地区晚第三纪孢粉植物群研究表明该植物群属北极第三纪植物区系,孢粉组合以被子植物为主,湿带成分如Ａｌｒｎｕｓ,Ｂｅｔｕｌａ、Ｃａｒｐｉｎｕｓ、Ｃｏｒｙｈｌｕｓ、Ｑｕｅｔｃｕｓ、Ｃａｓｔａｎｅａ、Ｕｌｍｕｓ等,亚热带、４喧成分劭Ｃｙｃｌｏｂａｌａｎｏｐｓｉｓ,Ｃａｓｔａｎｏｐｓｉｓ、Ｌｏｑｕｉｄａｍｂａｒ、Ｃａｒｙａ、Ｄｎｉｄｉａ、ＨＥＹ、ｐａｌｍａｅ等。吕合附近海拔较高的地带分布松科Ａｂｉｅｓ、     

中国竹类二新种及某些种类花的补充描述

本文记载了中国西南牡竹属二新种,席竹ＤｅｎｄｒｏｃａｌａｍｕｓｅｘｉｌｉｓＸｉａｅｔＣｈｉａ,钓鱼竹ＤｅｎｄｒｏｃａｌａｍｕｓｇｕｉｙａｎｇｅｎｓｉｓＸｉａｅｔＣｉａ,并对妈竹ＢａｍｂｕｓａｂｏｎｉｏｐｓｉｓＭｃＣｌ．、大眼竹Ｂ．ｅｕｔｕｌｄｏｉｄｅｓＭｃＣｌ．、撑篙竹Ｂ．ｐｅｒｖａｒｉａｂｉｌｉｓＭｃＣｌ．、紫秆竹Ｂ．ｔｅｘｔｉｌｉｓＭｃＣｌ．ｃｖ．Ｐｕｒｐｕｒａｓｃｅｎｓ和佛肚竹Ｂ．ｖｅｎｔｒｉｃｏｓａＭｃＣｌ．的花作了补充描述．     

National Museum of Plant History of China

ＴｈｅＮａｔｉｏｎａｌＭｕｓｅｕｍｏｆＰｌａｎｔＨｉｓｔｏｒｙｏｆＣｈｉｎａｉｓａｂｒａｎｃｈｏｆｈｅｒｂａｒｉｕｍ,ＩｎｓｔｉｔｕｔｅｏｆＢｏｔａｎｙ,ｔｈｅＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ．Ｉｔｗａｓｂｕｉｌｔｉｎ１９９６ａｎｄｏｆｆｉｃｉａｌｌｙａｐｐｒｏｖｅｄｂｙｔｈｅＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓｉｎ１９９８．ＩｔｗａｓｉｎｓｃｒｉｂｅｄｂｙＺｈｏｕＧｕａｎｇｚｈａｏ,Ｔｈｅｖｉｃｅ -ＣｈａｉｒｍａｎｏｆＳｔａｎｄｉｎｇＣｏｍｍｉｔｔｅｅｏｆＣｈｉｎｅ…     

中国栉Jia属五新种（拟步Jia科：朽木Jia亚科）

本文描述了拟步Ｊｉａ科,朽木Ｊｉａ亚科,栉Ｊｉａ属（ＣｔｅｎｉｏｐｉｎｕｓＳｅｉｄｌｉｔｚ,１８９６）的五个新种,光滑栉ＪｉａＣｔｅｎｉｏｐｉｎｕｓｇｌａｂｒａｔｕｓｓｐ．ｎｏｖ;隆背栉ＪｉａＣｔｅｎｉｏｐｉｎｕｓｐｒｏｔｕｂｅｒａｎｓｓｐ．ｎｏｖ,棕毛栉ＪｉａＣｔｅｎｉｏｐｉｎｕｓｂｒｕｎｎｅｉｃａｐｉｌｕｓｓｐ．ｎｏｖ异点栉ＪｉａＣｔｅｎｉｏｐｉｕｎｓｄｉｖｅｒｓｉｐｕｎｃｔａｔｕｓｓｐ．ｎｏ     

异细胞质信息八倍体小偃麦（Trititrigia 8x）的选育及其性状与细胞遗传

本实验用普通小麦“中国春”和八种异细胞质“中国春”（Ａｅｇｉｌｏｐｓ ｖａｖｉｌｏｖｉｉ）ＣＳ,（Ａｅ．ｊｕｖｅｎａｌｉｓ）ＣＳ,（Ａｅ．ｃｒａｓｓａ）ＣＳ,（Ａｅ．ｃｏｍｏｓａ）ＣＳ,（Ａｅ．ｕｎｉａｒｉｓｔａｔａ）ＣＳ,（Ａｅ．ｓｐｅｌｔｏｉｄｅｓ．Ｍ．）ＣＳ,（Ａｅ．ｋｏｔｓｃｈｙｉ）ＣＳ．（Ｔ．ｔｉｍｏｐｈｅｅｖｉ）ＣＳ分别与八倍体小偃麦（Ｔｒｉｔｉｔｒｉｇｉａ ８ｘ）“远中２”、“远中４     

毛药山茶——中国广东山茶属一新种

毛药山茶　新种　图 1ＣａｍｅｌｌｉａｒｅｎｓｈａｎｘｉａｎｇｉａｅＣ .Ｘ .ＹｅｅｔＸ .Ｑ .Ｚｈｅｎｇ ,ｓｐ .ｎｏｖ . (Ｓｕｂｇｅｎ .ＭｅｔａｃａｍｅｌｌｉａＨ .Ｔ .Ｃｈａｎｇ,Ｓｅｃｔ.ＥｒｉａｎｄｒｉａＣｏｈｅｎ Ｓｔｕａｒｔ) .Ｆｉｇ .1ＳｐｅｃｉｅｓＣ .ｃｒａｔｅｒａｅＨ .Ｔ .ＣｈａｎｇｅｔＣ .ｔｒｉｇｏｎｏｃａｒｐａｅＨ .Ｔ .Ｃｈａｎｇａｆｆｉｎｉｓ,ｓｅｄｉｌｌａｆｌｏｒｉｂｕｓｒｕｂｒｉｓ (ｔｅｓｔｅｎｏｔｕｌａｃｏｌｌｅｃｔｏｒｉｓ) ,ｂｒａｃｔｅｏｌｉｓｓｅｐａｌｉｓｑｕｅｕｔｒｉｎｑ…     

广西唇柱苣苔属一新种

融安唇柱苣苔　新种　图 1ＣｈｉｒｉｔａｒｏｎｇａｎｅｎｓｉｓＤ .ＦａｎｇｅｔＹ .Ｇ .Ｗｅｉ,ｓｐ .ｎｏｖ .(Ｃｈｉｒｉｔａｓｅｃｔ.ＧｉｂｂｏｓａｃｃｕｓＣ .Ｂ .Ｃｌａｒｋｅ) .Ｆｉｇ .1ＨａｂｉｔｕｓｉｍｉｌｉｓＣ .ｇｅｍｅｌｌａｅＤ .Ｗｏｏｄ ,ｑｕａｅｒｈｉｚｏｍａｔｅｓｔｏｌｏｎｉｆｅｒｏ ,ｆｏｌｉｉｓｅｌｌｉｐｔｉｃｉｓｖｅｌｏｂｏｖａｔｉｓｍｉｎｏｒｉｂｕｓｉｎｔｅｇｒｉｓ,ｃｙｍｉｓ 1～ 4＿ｆｌｏｒｉｓ,ｃｏｒｏｌｌａｅｔｕｂｉｏｒｅ 1 7ｍｍｄｉａｍ .,ｓｔａｍｉｎｕｍｆｉｌａｍｅｎｔｉｓｓ…     

里骆林区常绿阔叶林和人工杉木林气候水文效应

里骆林区常绿阔叶林和人工杉木林气候水文效应黄承标,文受春（广西农学院林学分院南宁５３０００１）（广西龙胜县林业局５４１７００）ＣｌｉｍａｔｉｃａｎｄＨｙｄｒｏｌｏｇｉｃａｌＥｆｆｅｃｔｓｏｆＥｖｅｒｇｒｅｅｎＢｒｏａｄ－ｌｅａｖｅｄａｎｄＡｒｔｉｆｉｃｉａｌＣｈｉｎｅｓｅＦｉｒＦｏｒｅｓｔｓｉｎＬｉｌｕｏＦｏｒｅｓｔｅｄＲｅｇｉｏｎ￥ＨｕａｎｇＣｈｅｎｇｂｉａｏ（ＦｏｒｅｓｔｒｙＣｏｌｌｅｇｅ,ＣｕａｎｇｘｉＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ,Ｎａｎｎｉｎｇ５３０００１）,ＷｅｎＳｈｏｕｃｈｕｎ（ＦｏｒｅｓｔｒｙＢｕｒｅａｕ,ＬｏｎｇｓｈｅｎｇＣｏｕｎｔｙ,Ｇｕａｎｇｘｉ５４１７００）;ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌ－ｏｇｙ,１９９３,１２（３）：１－７．Ｓｉｔｅｉｎｖｅｓｔｉｇａｔｉｏｎｓａｎｄｒｅｓｅａｒｃｈｅｓｉｎ１９８０－１９９０ｓｈｏｗｔｈａｔｅｖｅｒｇｒｅｅｎｂｒｏａｄ－ｌｅａｖｅｄａｎｄａｒｔｉｆｉｃｉａｌＣｈｉｎｅｓｅｆｉｒｆＯｒｅｓｔｓｉｎＬｉｌｕｏｆｏｒｅｓｔｅｄｒｅｇｉｏｎｃａｎｒｅｄｕｃｅｔｈｅｖａｒｉａｔｉｏｎｒａｎｇｅｏｆａｔｍｏｓｐｈｅｒｉｃａｎｄｓｏｉｌｔ     

Expression and self-regulatory function of cardiac interleukin-6 during endotoxemia

Interleukin (IL)-6 reportedly has negative inotropic and hypertrophic effects on the heart. Here, we describe endotoxin-induced IL-6 in the heart that has not previously been well characterized. An intraperitoneal injection of a bacterial lipopolysaccharide into C57BL/6 mice induced IL-6 mRNA in the heart more strongly than in any other tissue examined. Induction of mRNA for two proinflammatory cytokines, IL-1beta and tumor necrosis factor (TNF)-alpha, occurred rapidly before the induction of IL-6 mRNA and protein. Although stimulation of isolated rat neonatal myocardial cells with IL-1beta or TNF-alpha induced IL-6 mRNA in vitro, nonmyocardial heart cells produced higher levels of IL-6 mRNA upon stimulation with IL-1beta. In situ hybridization and immunohistochemical analyses localized the IL-6 expression primarily in nonmyocardial cells in vivo. Endotoxin-induced expression of cardiac IL-1beta, TNF-alpha, and intercellular adhesion molecule 1 was augmented in IL-6-deficient mice compared with control mice. Thus cardiac IL-6, expressed mainly by nonmyocardial cells via IL-1beta action during endotoxemia, is likely to suppress expression of proinflammatory mediators and to regulate itself via a negative feedback mechanism.     

竞争性PCR检测MLC_2-糜酶融合基因在转基因小鼠中的组织特异性表达

 用竞争性 Ｐ Ｃ Ｒ 方法定量检测了大鼠肌球蛋白轻链 ２ 启动子（ｍ ｙｏｓｉｎ ｌｉｇｈｔ ｃｈａｉｎ ２ ｐｒｏｍ ｏｔ ｅｒ, Ｍ Ｌ Ｃ２） 糜酶（ｃｈｙｍ ａｓｅ）融合基因在转基因小鼠不同组织中的表达情况,发现 Ｍ Ｌ Ｃ２ ｃｈｙｍ ａｓｅ融合基因在转基因小鼠的心脏中有较高水平的表达,在骨骼肌中表达水平较低,在肾脏中有微弱表达;而在肝脏和肺中未检测到．表明 Ｍ Ｌ Ｃ２ ｃｈｙｍ ａｓｅ 融合基因改变了野生型 ｃｈｙｍ ａｓｅ 在生物体内的表达特性,不仅提高了表达效率,而且赋予了一定的心脏组织特异性,为进一步研究 ｃｈｙｍ ａｓｅ 基因在心脏中的功能奠定了基础．     

Islet1基因与心脏发育

心脏发育及心脏疾病干细胞的治疗要求对心脏发育过程中的控制细胞增殖及分化的相关基因的作用机制进行深入了解.Islet1基因(Isl1基因)含有6个外显子和5个内含子,定位于人类5号染色体5q11.2.该基因在基因组内约占12kb,目前所知其最长可读框(ORF)至少由5个外显子组成,编码一个由384个氨基酸组成的转录因子蛋白.最近研究发现,不同的心脏细胞可能源于同一种多能心脏祖细胞—Isl1+细胞,心脏的这一发育模式与血液细胞的形成模式非常相像.另外有研究结果显示,Isl1是与心脏发育密切相关的转录因子之一,其表达随着心脏发育成熟而逐渐下调.虽然针对Isl1基因做了较多的研究工作,但是它表达调控的具体模式及发挥功能的详细作用机制目前仍未完全清楚,本文对最近几年Isl1基因的研究进展作一综述.     

CH50真核表达载体pCH503的构建及小鼠体内表达的趋化与抗肿瘤活性

构建重组 FN多肽 CH50真核表达载体并在小鼠体内表达 ,研究其趋化与抗肿瘤作用 .采用重组 DNA技术构建表达质粒 ;体内进行基因转染 ,采用 RT- PCR鉴定导入基因的表达 ;通过肝素亲和层析、SDS- PAGE和 Western blot鉴定表达产物 ;腹腔细胞计数、Giemsa染色分析以及肌肉组织切片与染色观察体内基因转染后的趋化作用 ;小鼠黑色素瘤模型研究基因转染抑制肿瘤的作用 .从 CH50原核表达载体获得重组多肽的 c DNA,5′端加上小鼠 IFN- 5′端非编码区和信号肽编码区的 c DNA,3′端加上人 FN c DNA的 3′端非编码区 ;将重组 c DNA插入 p REP8质粒 ,即构建出p CH50 3质粒 .巨噬细胞在体内经 p CH50 3转染 ,然后在体外培养 ,能够产生 CH50多肽 .以p CH50 3分别进行腹腔基因转染和肌肉内基因转染 ,均可对免疫细胞产生趋化作用 ;p CH50 3体内转染可以使小鼠腹腔内黑色素肿瘤结节数降低 50 %～ 60 % . CH50真核表达载体 p CH50 3可在小鼠体内表达 ,体内基因转染可趋化免疫细胞和抑制肿瘤结节形成 ,在肿瘤综合治疗中有重要意义 .     

Haploinsufficiency of TAB2 Causes Congenital Heart Defects in Humans

Congenital heart defects (CHDs) are the most common major developmental anomalies and the most frequent cause for perinatal mortality, but their etiology remains often obscure. We identified a locus for CHDs on 6q24-q25. Genotype-phenotype correlations in 12 patients carrying a chromosomal deletion on 6q delineated a critical 850 kb region on 6q25.1 harboring five genes. Bioinformatics prioritization of candidate genes in this locus for a role in CHDs identified the TGF-β-activated kinase 1/MAP3K7 binding protein 2 gene (TAB2) as the top-ranking candidate gene. A role for this candidate gene in cardiac development was further supported by its conserved expression in the developing human and zebrafish heart. Moreover, a critical, dosage-sensitive role during development was demonstrated by the cardiac defects observed upon titrated knockdown of tab2 expression in zebrafish embryos. To definitively confirm the role of this candidate gene in CHDs, we performed mutation analysis of TAB2 in 402 patients with a CHD, which revealed two evolutionarily conserved missense mutations. Finally, a balanced translocation was identified, cosegregating with familial CHD. Mapping of the breakpoints demonstrated that this translocation disrupts TAB2. Taken together, these data clearly demonstrate a role for TAB2 in human cardiac development.     

BMP2 is required for early heart development during a distinct time period

BMP2, like its Drosophila homologue dpp, is an important signaling molecule for specification of cardiogenic mesoderm in vertebrates. Here, we analyzed the time-course of BMP2-requirement for early heart formation in whole chick embryos and in explants of antero-lateral plate mesoderm. Addition of Noggin to explants isolated at stage 4 and cultured for 24 h resulted in loss of NKX2.5, GATA4, eHAND, Mef2A and vMHC expression. At stages 5-8 the individual genes showed differential sensitivity to Noggin addition. While expression of eHAND, NKX2.5 and Mef2A was clearly reduced by Noggin vMHC was only marginally affected. In contrast, GATA4 expression was enhanced after Noggin treatment. The developmental period during which cardiac mesoderm required the presence of BMP signaling in vivo was assessed by implantation of Noggin expressing cells into stage 4-8 embryos which were then cultured until stage 10-11. Complete loss of NKX2.5 and eHAND expression was observed in embryos implanted at stages 4-6, and expression was still suppressed in stages 7 and 8 implanted embryos. GATA4 expression was also blocked by Noggin at stage 4, however increased at stages 5, 6 and 7. Explants of central mesendoderm, that normally do not form heart tissue were employed to study the time-course of BMP2-induced cardiac gene expression. The induction of cardiac lineage markers in central mesendoderm of stage 5 embryos was distinct for different genes. While GATA4, -5, -6 and MEF2A were induced to maximal levels within 6 h after BMP2 addition, eHAND and dHAND required 12 h to reach maximum levels of expression. NKX2.5 was induced by 6 h and accumulated over 48 h. vMHC and titin were induced at significant levels only after 48 h of BMP2 addition. These results indicate that cardiac marker genes display distinct expression kinetics after BMP2 addition and differential response to Noggin treatment suggesting complex regulation of myocardial gene expression in the early tubular heart.     

The Human Mast Cell Chymase Gene (CMA1): Mapping to the Cathepsin G/Granzyme Gene Cluster and Lineage-Restricted Expression

Genes encoding T-cell-receptor α/δ chains, neutrophil cathepsin G, and lymphocyte CGL/granzymes are closely linked on chromosomal band 14q11.2. The current work identifies the human mast cell chymase gene (CMA1) as the fourth protease in this cluster and maps the gene to within 150 kb of the cathepsin G gene. The gene order is centromere-T cell receptor α/δ-CGL-1/granzyme B-CGL-2/granzyme H-cathepsin G-chymase. Chymase and cathepsin G genes are shown to be cotranscribed in the human mast cell line HMC-1 and in U-937 cells. Other cells transcribe cathepsin G or CGL/granzyme genes, but not chymase genes, suggesting a capacity for independent regulation. Comparison of the 5′ flank of the chymase gene with those of cathepsin G and CGL/granzymes reveals little overall homology. Only short regions of the 5′ flanks of the human and murine chymase genes sequenced to date are similar, suggesting that they are more distantly related than human and rodent CGL-1/granzyme B, the flanks of which are highly homologous. The expression patterns and clustering of genes provide possible clues to the presence of locus control regions that orchestrate lineage-restricted expression of leukocyte and mast cell proteases.