FAM3B PANDER and FAM3C ILEI Represent a Distinct Class of Signaling Molecules with a Non-Cytokine-like Fold

1. Download : Download high-res image (246KB)
2. Download : Download full-size image

Highlights? Contrary to previous predictions FAM3B PANDER exhibits a new non-cytokine-like fold ? The new fold is also conserved in FAM3C ILEI and involved in cancer and tumor formation ? FAM3 is a new class of signaling molecules different from currently known cytokines     

Myosin-IXA regulates collective epithelial cell migration by targeting RhoGAP activity to cell-cell junctions

1. Download : Download high-res image (204KB)
2. Download : Download full-size image

Highlights? Myosin-IXA, an actin-motor- and RhoGAP-protein, is required for collective migration ? Myosin-IXA is required for radial actin bundle assembly at nascent cell-cell contacts ? The localization of myosin-IXA to actin bundles is dependent on its motor domain ? Myosin-IXA RhoGAP domain locally inhibits Rho stabilizing nascent cell-cell contacts     

SorLA Controls Neurotrophic Activity by Sorting of GDNF and Its Receptors GFRα1 and RET

1. Download : Download full-size image

Highlights? SorLA is a sorting receptor for GDNF and its signaling receptors GFRa1 and RET ? The SorLA/GFRa1 complex targets GDNF for lysosomal degradation, while GFRa1 is recycled ? SorLA/GFRa1 targets RET for endocytosis and influences GDNF-induced neurotrophic effects ? SorLA knockout mice display altered dopaminergic function and an ADHD-like phenotype     

Tracking Shallow Chemical Gradients by Actin-Driven Wandering of the Polarization Site

1. Download : Download high-res image (305KB)
2. Download : Download full-size image

Highlights? A wandering polarity site distributes growth in yeast cells responding to pheromone ? Wandering, but not polarity establishment, is actin dependent ? A mathematical model incorporating polarity and vesicle traffic exhibits wandering ? Blocking wandering impairs gradient tracking     

IKKε regulates cell elongation through recycling endosome shuttling

1. Download : Download high-res image (412KB)
2. Download : Download full-size image

Highlights? Recycling endosomes shuttle along the proximal-distal axis of growing bristles ? IKK? locally antagonizes Rab11 effector Nuf at the bristle tip by phosphorylation ? Nuf-endosome pathway is independent of actin cytoskeleton or DIAP1-caspase pathway ? Mammalian IKK-related kinases antagonize Rab11-FIP3 through phosphorylation     

Keratocyte Fragments and Cells Utilize Competing Pathways to Move in Opposite Directions in an Electric Field

1. Download : Download high-res image (338KB)
2. Download : Download full-size image

Highlights? A cell fragment is the simplest motile electric-field-sensing unit ? Keratocyte cells and fragments migrate in opposite directions in an electric field ? Competition of two signal transduction pathways underlies keratocyte galvanotaxis ? Protrusive and contractile actin networks are steered by the electric field     

Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval

1. Download : Download high-res image (327KB)
2. Download : Download full-size image

Highlights? Targeted RNAi screen of adaptors for vesicle retrieval at hippocampal synapses ? Stonin 2 is a major adaptor for clathrin-mediated retrieval of synaptic vesicles ? Rapid, induced rerouting of stonin 2 demonstrates the role of this adaptor     

CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine expression on apoptotic and phagocytic cells

1. Download : Download high-res image (120KB)
2. Download : Download full-size image

Highlights? PS is expressed on the surface of apoptotic and phagocytic cells during apoptosis ? CED-7 and TTR-52 mediate time-dependent loss of PS from surface of apoptotic cells ? CED-7 and TTR-52 promote generation of extracellular PS vesicles ? CED-7/TTR-52/CED-1 promote phagocyte PS expression, important for corpse engulfment     

Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte

1. Download : Download high-res image (370KB)
2. Download : Download full-size image

Highlights? Merozoite attachment signals commitment to invasion and triggers subsequent events ? PfAMA1 and PfRON4 define core components of the merozoite-erythrocyte tight junction ? A ring of actin forms at the tight junction midway through merozoite invasion     

Interplay between the Dividing Cell and Its Neighbors Regulates Adherens Junction Formation during Cytokinesis in Epithelial Tissue

1. Download : Download high-res image (261KB)
2. Download : Download full-size image

Highlights? During cytokinesis, neighboring cells accumulate MyoII at the edges of the furrow ? MyoII nonautonomously sets the initial geometry of the daughter cell interface ? Neighboring membranes impede adherens junction (AJ) formation until a midbody forms ? Arp2/3-dependent actin accumulation in the dividing cell maintains AJ geometry     

Distinct and Overlapping Roles for AP-1 and GGAs Revealed by the “Knocksideways” System

1. Download : Download high-res image (206KB)
2. Download : Download full-size image

Highlights? AP-1 knocksideways depletes ～100 proteins from clathrin-coated vesicles (CCVs) ? GGA2 knocksideways mainly depletes hydrolases and their receptors ? GGA2 depends on AP-1 for incorporation into CCVs ? AP-1 acts as a linchpin for intracellular CCV formation and is bidirectional     

Aurora B regulates formin mDia3 in achieving metaphase chromosome alignment

1. Download : Download high-res image (196KB)
2. Download : Download full-size image

Highlights? Involvement of formin mDia3 in stable kinetochore microtubule attachment ? The actin activity of mDia3 is not necessary for metaphase chromosome alignment ? EB1-binding by mDia3 contributes to position chromosomes at the metaphase plate ? Aurora B phosphorylates mDia3 to regulate its microtubule activity     

HIV Nef,Paxillin, and Pak1/2 Regulate Activation and Secretion of TACE/ADAM10 Proteases

1. Download : Download high-res image (214KB)
2. Download : Download full-size image

Highlights? HIV-Nef translocates activated TACE/ADAM17 into extracellular vesicles (EVs) ? The mechanism is initiated by interaction of Eed and paxillin with TACE ? Translocation of TACE into EVs is regulated by Pak2 ? Melanoma cells activate the same mechanism to upload activated ADAM10 into EVs     

HRG1 Is Essential for Heme Transport from the Phagolysosome of Macrophages during Erythrophagocytosis

1. Download : Download high-res image (247KB)
2. Download : Download full-size image

Highlights? Humans have about 25 trillion RBCs, each containing over a billion heme moieties ? Five million senescent RBCs are recycled per second by erythrophagocytosis in macrophages to make new RBCs ? HRG1, the mammalian homolog of C. elegans heme transporter, recycles heme-iron from the macrophage phagolysosome ? Missense polymorphisms in human HRG1 may be genetic modifiers of iron metabolism     

ADF/cofilin regulates secretory cargo sorting at the TGN via the Ca2+ ATPase SPCA1

1. Download : Download high-res image (239KB)
2. Download : Download full-size image

Highlights? ADF/cofilin localizes to the TGN and binds SPCA1 in an actin-dependent manner ? Dynamic actin regulates the activity of the Ca²⁺ pump SPCA1 ? ADF/cofilin facilitates secretory cargo sorting in a Ca²⁺-dependent manner     

Calponin 2 Acts As an Effector of Noncanonical Wnt-Mediated Cell Polarization during Neural Crest Cell Migration

1. Download : Download full-size image

Highlights? Cnn2 is expressed in NCCs and required for their migration in frogs and chicks ? Cnn2 is inactivated by noncanonical Wnt signaling ? Loss of Cnn2 causes a switch from cortical actin to central stress fibers ? Cnn2 polarizes the actin cytoskeleton downstream of PCP     

Structural Mechanism of CCM3 Heterodimerization with GCKIII Kinases

1. Download : Download high-res image (406KB)
2. Download : Download full-size image

Highlights? Crystal structure of CCM3-MST4 heterodimeric complex ? Structural mechanism driving CCM3-GCKIII heterodimerization ? Conformational changes required for CCM3-GCKIII heterodimerization ? Synergistic effects of CCM3-MST4 complex on cell proliferation and migration     

Differential Regulation of Distinct Vps34 Complexes by AMPK in Nutrient Stress and Autophagy

1. Download : Download high-res image (245KB)
2. Download : Download full-size image

Highlights? Different Vps34 complexes are distinctly regulated upon energy stress ? AMPK activates the proautophagy Vps34 complex by phosphorylating Beclin1 ? AMPK inhibits the nonautophagic Vps34 complex by phosphorylating Vps34 ? ATG14L determines whether the Vps34 complex is activated or inhibited by AMPK