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On the thermal denaturation of glucose oxidase 总被引:5,自引:0,他引:5
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The result of experiments in a fixed-bed reactor containing glucose oxidase immobilized on a nonporous support and conducted in the absence of diffusional limitations are reported. Kinetic parameters were established by separate batch experiments. The key observation was that, in every case, poisoning by product hydrogen peroxide resulted in a minimum in enzyme activity in the interior of the bed, well away from the ends. The deactivation data were interpreted by fitting the rate constant for poisoning, the only free parameter, to a previously reported theory. The theory postulates several deactivation mechanisms each of which leads to self-consistent kinetics, but the only mechanism which fitted the data assumes that peroxide attack the enzyme when it is the from complexed with glucose. Theory and experiment agreed to within the accuracy (+/- 2%) of the activity measurements. 相似文献
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Properties of glucose oxidase 总被引:8,自引:0,他引:8
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The electrophoretic deposition of glucose oxidase from water using asymmetrical alternating voltages is investigated. Using asymmetric voltages, glucose oxidase layers with a thickness of 7 μm could be deposited on a platinum electrode in 20 min time as verified with a microbalance, carbon analysis and scanning electron microscopy. In contrast, if a symmetrical alternating signal is used under the same conditions, a layer of 0.5 μm is formed. We believe the deposition is due to two effects: the electrophoretic migration of the enzyme towards the deposition electrode and the pH induced precipitation of the enzyme near the deposition electrode. The electrophoretic migration is due to the non-linear dependence of the electrophoretic mobility on the electric field caused by the asymmetry of the applied alternating current signal. In addition, pH changes near the deposition electrode drive the enzyme towards its point of zero charge (PZC), perhaps causing the precipitation of GOx on the substrate. The effect of amplitude, frequency, deposition time and GOx concentration on the deposition rate was studied. An amplitude of 160 Vp–p and a frequency of 30 Hz was found to be optimal for the formation of thick enzyme layers, which excludes a big part of the interferences. 相似文献
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The oxidation of 1-deuterated glucose by glucose oxidase 总被引:3,自引:0,他引:3
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Studies have been performed in a tubular flow reactor to characterize the deactivation of immobilized glucose oxidase. The effects of oxygen concentration in the range of 0.09 to 0.467mM and hydrogen peroxide concentrations in the range of 0.1 to 10mM were studied. A simple mathematical model assuming first-order reaction and deactivation was found to describe the deactivation behavior adequately. The deactivation rate constant was found to increase with increasing levels of feed oxygen. Hydrogen peroxide was found to deactivate the enzyme severely and the deactivation rate constants were higher than those for oxygen deactivation. The influence of external and internal diffusion effects on the deactivation rate constant were examined. Although diffusional restrictions were negligible for oxygen transfer to the pellet, they were significant for transfer of hydrogen peroxide to the bulk stream. Increasing deactivation rates. Severe internal diffusion limitations were observed for the glucose oxidase system. However, for particle sizes in the range of 500 to 2000 μm, no effect on the rate of deactivation of the enzyme was observed. 相似文献
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Leskovac V Trivić S Pericin D Kandrac J 《Journal of enzyme inhibition and medicinal chemistry》2004,19(2):169-174
A novel reaction of cytochrome-c from the horse heart with the enzyme glucose oxidase from Aspergillus niger (EC 1.1.3.4), in acidic media is described. Glucose oxidase is able to induce a rapid, profound and irreversible physico-chemical change in cytochrome-c, under anaerobic conditions and in the presence of glucose. The initial rate of reaction is almost independent of the concentration of enzyme and glucose. The striking feature of this reaction is the fact that the reaction proceeds efficiently even below a concentration of 10 nM enzyme. 相似文献
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Meso-tetra(4-carboxyphenyl)porphine (CTPP(4)) binds reversibly to immobilized glucose oxidase (GOD), resulting in an absorbance peak for the CTPP(4)-GOD complex at 427nm. The absorbance intensity of the 427nm peak is reduced upon exposure to glucose, which causes the dissociation of CTPP(4) from GOD. The change in absorbance at 427nm shows linear dependence on glucose concentration from 20 to 200mg/dL (1.1-11.1mM). 相似文献
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A polyethylene-g-acrylic acid (PE-g-AA) graft copolymer was prepared via gamma-ray-irradiation-induced postirradiation procedures, and was used as support material for the immobilization of glucose oxidase. Soluble carbodiimides were used as the coupling agent. Reasonable yields were obtained with CMC but not with EDAC, EEDQ, or WRK. A number of factors were studied. (1) The use of water-soluble carbodiimides as condensing agent was attempted and the optimum condition for coupling glucose oxidase to PE-g-AA was established; (2) the effect of pH and temperature on the reactivity of native and immobilized glucose oxidase was studied. When exposed to temperatures in excess of 60 degrees C, the immobilized glucose oxidase was less sensitive to thermal inactivation than the native enzyme. The optimum pH value for the performance of the enzyme-immobilized membrane was 5. 6. For 200 tests, the response error of glucose sensor was less than 4% and its linear detected range was 0-1000 ppm. The obtained glucose oxidase-immobilized PE-g-AA membranes were kept in pH 5. 6 acetate buffer solution at 4 degrees C. The glucose oxidase activity of the membrane was determined at sevenday intervals. The membranes still have 92% glucose oxidase activity even after eight weeks of storage. 相似文献
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A theoretical study of the dioxygen activation by glucose oxidase and copper amine oxidase 总被引:4,自引:0,他引:4
Glucose oxidase (GO) and copper amine oxidase (CAO) catalyze the reduction of molecular oxygen to hydrogen peroxide. If a closed-shell cofactor (like FADH(2) in GO and topaquinone (TPQ) in CAO) is electron donor in dioxygen reduction, the formation of a closed-shell species (H(2)O(2)) is a spin forbidden process. Both in GO and CAO, formation of a superoxide ion that leads to the creation of a radical pair is experimentally suggested to be the rate-limiting step in the dioxygen reduction process. The present density functional theory (DFT) studies suggest that in GO, the creation of the radical pair induces a spin transition by spin orbit coupling (SOC) in O(2)(-)(rad), whereas in CAO, it is induced by exchange interaction with the paramagnetic metal ion (Cu(II)). In the rate-limiting step, this spin-transition is suggested to transform the O(2)(-)(rad)-FADH(2)(+)(rad) radical pair in GO and the Cu(II)-TPQ (triplet) species in CAO, from a triplet (T) to a singlet (S) state. For CAO, a mechanism for the O[bond]O cleavage step in the biogenesis of TPQ is also suggested. 相似文献