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The RLGS (Restriction Landmark Genome Scanning) method was originally developed as a powerful method for enabling viewing of thousands of restriction landmarks. It offers a tool for obtaining information about genetic loci, with a single RLGS profile displaying approximately 2000 restriction landmarks as spots. One of the most useful applications is RLGS spot mapping, which allows the efficient, low-cost construction of the genetic map of any organism. However, analyses of the profiles depend mainly on human visual observation and are tedious and laborious. Although several commercially available image analyzing systems for profile comparison have been examined, they cannot be used for the RLGS spot mapping system owing to the background characteristics of the RLGS profiles, unsatisfactory rates of correspondence, and inefficient correction of informative genetic data. We therefore developed a novel automatic image analysis system for RLGS spot mapping, using an original algorithm based on the binary image transferred from the original RLGS profile. This system was employed for identifying non-polymorphic and parental strain-specific polymorphic spots of the F1 mouse profile and yielded efficient initial screening of RLGS profiles. Received: 5 December 1997 / Accepted: 6 April 1998  相似文献   

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谷氨酸发酵过程葡萄糖自动流加系统   总被引:1,自引:0,他引:1  
补料(流加葡萄糖)操作是谷氨酸发酵过程最重要的操作之一,工业上有各种各样的补料操作方法。控制葡萄糖浓度于一个较为平稳且适中的水平有利于提高谷氨酸发酵的性能指标。通过在线计量谷氨酸发酵中的氨水耗量并据此在线推定发酵液中的葡萄糖浓度,构建了一个谷氨酸发酵自动在线补料系统。使用该控制系统,谷氨酸发酵过程的葡萄糖浓度可以控制在任意水平,平稳、无波动的谷氨酸发酵可以得到实现。  相似文献   

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An automatic high-resolution gradient analyzing system   总被引:3,自引:0,他引:3  
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An algorithm for automatic genotype elimination.   总被引:9,自引:4,他引:9  
Automatic genotype elimination algorithms for a single locus play a central role in making likelihood computations on human pedigree data feasible. We present a simple algorithm that is fully efficient in pedigrees without loops. This algorithm can be easily coded and has been instrumental in greatly reducing computing times for pedigree analysis. A contrived counter-example demonstrates that some superfluous genotypes cannot be excluded for inbred pedigrees.  相似文献   

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Summary Computer-assisted metaphase analysis for prenatal diagnosis can be economically performed by a preexisting hardware-software system. The program can be run by technician-level (i.e., nonspecialist) personnel who can opt for the automatic operational mode for good quality metaphases or for the semimanual mode for problem metaphases (numerous superpositions, artifacts, etc.). The karyotype can be obtained in conversational mode. Hardcopy output is available for all modes.  相似文献   

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目的:建立一种自动控制颈动脉窦区灌流压的新方法。方法:采用AT89C2051单片计算机构成谦价实用的灌流控制器,钭它与LDB-M型电子蠕动泵、实验动物的颈动脉窦区构成自动灌流系统。结果及结论:该系统能对大鼠颈动脉窦区进行均匀斜坡升压,阶梯升压-降压灌流,并已用于压力感受器反射效应研究中,此系统能保证灌流曲线的准确性和可重复性。进一步提高了实验的技术水平。  相似文献   

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ABSTRACT. An improved electroantennogram (EAG) apparatus with a new automatic sample injection system, based on a cyclic timer connected to a 12VD.C. electrovalve, is described. The device is fully automatic and allows insertion of constant cyclic air puffs of the test compounds to the antenna at predetermined intervals.  相似文献   

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烟粉虱成虫密度自动计数系统   总被引:2,自引:0,他引:2  
为改善烟粉虱Bemisia tabaci(Gennadius)种群密度调查的手段,根据图像识别原理,形成了一套田间烟粉虱成虫密度自动计数系统,该系统通过成像装置采集烟粉虱成虫的图像,测量成像装置与成像对象之间的距离,对图像中的烟粉虱图像进行识别并计数,计算寄主植物叶面积,最后得出烟粉虱成虫密度。应用该系统对烟粉虱成虫密度进行调查,准确率在90%以上。  相似文献   

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This study developed an artificial neural network (ANN) to estimate the growth of microorganisms during a fermentation process. The ANN relies solely on the cumulative consumption of alkali and the buffer capacity, which were measured on-line from the on/off control signal and pH values through automatic pH control. The two input variables were monitored on-line from a series of different batch cultivations and used to train the ANN to estimate biomass. The ANN was refined by optimizing the network structure and by adopting various algorithms for its training. The software estimator successfully generated growth profiles that showed good agreement with the measured biomass of separate batch cultures carried out between at 25 and 35_C.  相似文献   

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An automatic tracking system for rotifer swimming movements is described. The x and y coordinates of the Center of gravity of the animal are stored in the computer memory every twelfth of a second. Computer programs were developed to analyze the data and to calculate the average and the standard deviation for the following parameters per unit of time: distance traveled, turning angles toward the right and toward the left, distance from the point of departure as the crow flies. Histograms are traced by the computer showing the distribution of these values, as well as their evolution with time. This experimental system is dependable. The first values which we obtained for the swimming speed of rotifers correspond well with those which are given in the literature. The other parameters had not been measured until now.  相似文献   

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