人抗E．Coli J5噬菌体抗体制备的初步研究

以E．Coli J5株对合人Ig基因的噬菌体抗体库进行淘筛富集,免疫印迹筛选,以及ELISA检测,结果获得4株能与E．Coli J5株结合的阳性克隆,且阳性克隆结合抗原的活性可分别被E．Coli J5株、E．Coli Rc-LPS和抗E．Coli J5株核心糖脂域MAb抑制．PCR检测表明,4株阳性克隆均分别带有约660bp大小的重链和轻链基因片段．SDS－PAGE与蛋白质印迹的结果显示,经IPTG诱导的阳性克隆能表达分子量约为50000大小的蛋白,提示该4株阳性克隆能够表达具有一定抗原结合活性的人源Fab片段．     

BOOK REVIEWS

Book reviewed in this article:
E thnology and E thnography : Das religioese Weltbild einer fruehen Kultur . A dolf E. J ensen .
E thnology and E thnography : Mythos una Kult bei den Naturvoelkern . A dolf E. J ensen .     

Reviews

Plant Genetic Resources of Ethiopia. By J. M. M. E ngels , J. G. H awkes and M elaku W orede
Compartmentation of Plant Metabolism in Nonphotosynthetic Tissues. Ed. by M. J. E mes     

Book Reviews

Books reviewed in this article:
GENTLE, J. E. Numerical Linear Algebra for Applications in Statistics.
GENTLE, J. E. Random Number Generation and Monte Carlo Methods.
LOTKA, A. J. Analytical Theory of Biological Populations     

Interaction with proteoglycans enhances the sterol efflux produced by endogenous expression of macrophage apoE.

Endogenous expression of apolipoprotein (apo)E in macrophages facilitates cholesterol efflux in the presence and absence of extracellular sterol acceptors. A proteoglycan-associated pool of apoE has also been described. The relationship between a proteoglycan-associated pool of apoE and enhanced cholesterol efflux was investigated in these studies. Inhibition of proteoglycan expression reduced cholesterol efflux from apoE-expressing cells ( J774E(+)) in the presence and absence of HDL, but did not do so from nonexpressing cells ( J774E(-)). The effect of proteoglycan depletion on sterol efflux from J774E(+) cells was confirmed by measuring differences in cell sterol mass, secreted sterol mass, and sterol efflux rates. Furthermore, apoE-containing particles secreted from proteoglycan-depleted J774E(+) cells were denser than those secreted from J774E(+) cells with intact proteoglycan expression. Also, in J774E(+) cells with intact proteoglycans, apoE particles isolated from the cell surface proteoglycan layer were denser than secreted particles. The apoE-lipid particles isolated from the cell surface proteoglycan layer had a lower lipid-to-apoE and cholesterol-to-apoE ratio compared with secreted particles. In distinction, proteoglycan depletion of J774E(-) cells did not reduce sterol efflux produced by the exogenous addition of apoE. These observations indicate that one mechanism by which endogenous expression of apoE facilitates effective cholesterol efflux from macrophages is related to its retention at the cell surface in a proteoglycan-associated pool. Further, our data suggest that apoE arrives at the cell surface in a relatively lipid-poor state, and that a proximate source of lipid available to the proteoglycan-bound apoE at the cell surface resides in the plasma membrane.     

REVIEWS

Book reviewed in this article:
A Dictionary of the Flowering Plants and Ferns . By the late J. C. W illis . Seventh edition, revised by H. K. A iry S haw .
The Principles of Pollination Ecology . By K. F aegri and L. van D er P ijl .
Studies in Biology, No. 5, Plant Taxonomy . By V. H. H eywood .
Deadly Harvest . By J ohn M. K ingsbury .
Plant Physiology . By H enrik G. L undegardh . Translated by F. M. I rvine , Translation edited by W. O. J ames .
The Biology of Lichens . By M ason E. H ale J r . In: Contemporary Biology Series, General editors E. J. W. B arrington and A. J. W illis .
Plant Biology Today . Second edition. Ed. by W. A. J ensen and L. G. K avaljian .
Organisation in Plants . Second edition. By B. M. B aron .
Fortschritte der Botanik , Band 28. Ed. by E. B ünning , H. E llenberg , K. E sser , H. M erxmuller and P. S itte .
Taxonomy of Flowering Plants . Second edition. By C. L. P orter .
The Study of Biology . By J. J. W. B aker and G. E. A llen .
Experiments in the Study of Biology . By J. J. W. B aker , G. E. A llen , E. G age and S. K. W ebster .
Grundriss der Phytopathologie iind des Pflanzenschutzes . By H ans -A lfred K irchner .
Bibliography of Seeds . Compiled and edited by L. V. B arton .
Pollen Grains of Western Himalaxan Plants . By P. K. K. N air .
Families of Flowering Plants in Ethiopia . By W. C. B urger .     

REVIEWS

Book reviewed in this article
Growth Regulators in Plant Science, Monograph 8. Ed. by M. B. J ackson , B. G rout and I. A. M ackenzie .
An Introduction to Plant Taxonomy. By C. J effrey .
Pest and Diseases of Tropical Crops , Vol. 1. By D. S. H ill and J. M. W aller .
Flora of Turkey , Vol. 7 ( Orobanchaceae to Ceratophyllaceae and Ruhiaceae ). Ed. by P. H. D avis , assisted by J. R. E dmondson , R. R. M ill and K. T an .
Plant Growth Curves. The Functional Approach to Plant Growth Analysis. By R oderick H unt .
Bryophyte Ecology. Ed. by A. J. E. S mith . Bryophyte Ecology. Ed. by A. J. E. S mith . Bryophyte Ecology. Ed. by A. J. E. S mith . Bryophyte Ecology. Ed. by A. J. E. S mith .
Forest Succession: concepts and application. Ed. by D. C. W est , M. H. S hugart and D. B otkin .     

Genetic background modulates the phenotype of a mouse model of DYT1 dystonia

DYT1 dystonia is a debilitating neurological disease characterized by involuntary twisting movements. The disease is caused by an in-frame deletion (GAG, "ΔE") mutation in the TOR1A gene that encodes the torsinA protein. Intriguingly, only 30% of mutation carriers exhibit motor symptoms despite the fact that functional brain imaging studies show abnormal brain metabolism in all carriers. Because genetic modifiers may be a determinant of this reduced penetrance, we examined the genetic contribution of three different inbred strains of mice on the DYT1 mutation in animals that are homozygous (Tor1a(ΔE/ΔE)) or heterozygous (Tor1a(ΔE/+); disease state) for the disease-causing ΔE mutation. We find that the DBA/2J, C57BL/6J, and CD1-ICR contribution of genes significantly alter lifespan in Tor1a(ΔE/ΔE) mice, which die during the first few days of life on the 129S6/SvEvTac (129) background. The C57BL/6J (B6) strain significantly decreases life expectancy of Tor1a(ΔE/ΔE) animals but, like 129S6/SvEvTac Tor1a(ΔE/+) mice, congenic C57BL/6J Tor1a(ΔE/+) mice do not exhibit any motor abnormalities. In contrast, the DBA/2J (D2) strain significantly increases life expectancy. This effect was not present in congenic DBA/2J Tor1a(ΔE/ΔE) mice, indicating that the extended lifespan of F2 129/D2 mice was due to a combination of homozygous and heterozygous allelic effects. Our observations suggest that genetic modifiers may alter the penetrance of the ΔE mutation, and that mapping these modifiers may provide fresh insight into the torsinA molecular pathway.     

Book Reviews

Book reviewed in this article:
Australian Vegetation. (2nd edition) ed. R. H. Groves
Plant Resources of South East Asia Rattans, edited by J. Dransfield and N. Nanokaran
Plant Growth and Development. A Molecular Approach, by Donald E. Fosket
Potato Genetics, eds J. E. Bradshaw and G. R. Mackay
Alien Plants of the British Isles, by E. J. Clement and M. C. Foster     

Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli.

Mutations in Escherichia coli previously reported (R. E. Harkness and E. E. Ishiguro, J. Bacteriol. 155:15-21, 1983; L. C. Shimmin, D. Vanderwel, R. E. Harkness, B. R. Currie, A. Galloway, and E. E. Ishiguro, J. Gen. Microbiol. 130:1315-1323, 1984) as conferring a temperature-dependent tolerance to lysis induced by inhibitors of peptidoglycan synthesis were suppressed by treatment with inhibitors of the stringent response or by introduction of a relA mutation. The relA+ derivatives of the mutants exhibited a stringent response at the nonpermissive temperature. The consequent inhibition of the autolytic enzyme system (W. Kusser and E. E. Ishiguro, J. Bacteriol. 164:861-865, 1985) was apparently responsible for the lysis-tolerant phenotypes of these mutants.     

Buchbesprechungen / Book Reviews

Book reviews in this article:
Vanderplank, J. E. , Host-Pathogen Interactions in Plant Disease.
Vanderplank, J. E. , Host-Pathogen Interactions in Plant Disease.     

Effects of estradiol and estradiol sulfamate on the uterus of ovariectomized or ovariectomized and hypophysectomized rats

Estradiol sulfamate (J995), estradiol-17beta with a substituted sulfamate group in position 3, has much higher systemic estrogenic activity after oral administration than 17beta-estradiol (E2) due to reduced hepatic metabolism of the drug. The lower dose necessary for achievement of adequate systemic estrogenic effects results in a substantial reduction of otherwise commonly observed hepatic side-effects. This makes J995 a strong candidate as an estrogen suitable for oral administration. The present study was performed to examine and compare the effects of J995 and E2 on the uterus after oral or subcutaneous administration to ovariectomized or ovariectomized+hypophysectomized female rats, in particular on the levels of the estrogen receptor (ER) (alpha+beta), ERalpha mRNA and insulin-like growth factor-I (IGF-I) mRNA. The ER levels were determined using a ligand binding assay and the mRNA levels using solution hybridization. The doses of J995 or E2 were chosen to achieve comparable uterotrophic activity. The rats were treated with hormones for 7 days and the treatment was initiated 14 days after surgery. We conclude that there are no major differences in the uterine response to treatment with J995 or E2 with respect to the effects on ER and ERalpha mRNA levels. The IGF-I mRNA level though, is more affected by J995 than by E2 after 7 days of treatment, indicating a prolonged effect of J995.     

BOOK REVIEWS

Books reviewed in this article:
PHYSIOLOGY OF ELASMOBRANCH FISHES. Edited by T. J. Shuttleworth
FISH VACCINATION. Edited by A. E. Ellis
FISH POPULATION DYNAMICS, 2nd Edition. Edited by J. A. Gulland
ON LAMPREYS AND FISHES. Edited by Don E. McAllister and Edward Kott
SHARKS OF THE ORDER CARCHARHINIFORMES. By L. J. V. Compagno     

Effects of estradiol and estradiol sulfamate on the liver of ovariectomized or ovariectomized and hypophysectomized rats

This study was performed to evaluate and compare the effects of estradiol sulfamate (J995) and estradiol (E2) on the hepatic levels of the estrogen receptor (ER) and its mRNA, in ovariectomized (OVX) and OVX+hypophysectomized (OVXHX) female rats and to study the effects on the liver-derived serum compounds angiotensin I, triglycerides, high-density lipoprotein (HDL) and cholesterol. ER concentrations were determined using ligand-binding assay (LBA) and enzyme immuno assay (EIA), and the mRNA levels using solution hybridization.

The rats were treated orally (p.o.) or subcutaneously (s.c.) for 7 days, with treatments initiated 14 days after surgery.

No differences were found in ER mRNA levels between J995 and E2 treated rats.

The s.c. administered estrogens increased ER levels in OVX rats. Addition of GH+DEX to OVXHX rats restored the ER to levels above those seen in intact rats, whereas simultaneous oral treatment with E2 significantly decreased ER levels again. The s.c. treatment with either J995 or E2 limited the increase caused by addition of GH+DEX.

After oral treatment angiotensin I levels were increased by E2, but not by J995, while triglycerides, HDL and cholesterol levels were decreased by oral E2, J995 showing a similar pattern but was less effective.

In summary, these results on hepatic ER levels and estrogen dependent compounds produced by the liver showed that J995 has a lower impact on the normal liver functions after oral treatment than E2. Thus, J995 is a very promising substance for development of oral estrogen treatment with reduced hepatic side effects.     

REVIEWS

Books reviewed in this article:
The Control of Growth and Differentiation in Plants. By P. F. W areing and I. D. J. P hillips .
The Cuticles of Plants. By J. T. M artin and B. E. J uniper .
Vegetationszonen und Klima. By H einrich W alter .
Tertiäre Floren von Mähren. By E. K nobloch .
Drawings of British Plants. Part XXVII. Ulmaceae to Pinaceae. By S tella R oss C raig .
Seaweeds and their Uses. By V. J. C hapman .
Control of Organelle Development. Ed. by P. L. M iller .
Cell Biology. By J. W. K imball .
Phytochemical Phylogeny. Ed. by J. B. H arborne .
the study of botany. By P reston A dams , J effrey J. W. B aker and G arland E. A llen .
Little Botany, Big Botany. By D. H. J ennings .     

Matching unrelated stimuli with same discriminative functions: training order effects

Previous research has shown that after training simple discriminations (A1+/A2−, B1+/B2−), bringing these tasks under conditional control (J1–A1, J2–A2) leads to transfer of discriminative control (J1+/J2−) and to generalized matching on the basis of same discriminative functions (e.g. J1–B1, J2–B2). The same occurs when conditional discriminations are trained (D1–E1, D2–E2; F1–G1, F2–G2). When the subjects are then trained to demonstrate correct relations (D1–E1, D2–E2) when given X1 and to demonstrate incorrect relations when given X2 (XD–E), transfer of discriminative control (X1+/X2−) and generalized matching on the basis of same discriminative functions emerges (e.g. X1F1–G1, X2F1–G2). The present study investigated if these performances are dependent on the training and/or testing order. In Experiment 1, the lower-order contingency tasks were trained before the higher-order contingency tasks (A1+/A2−, B1+/B2− before J–A, and D–E, F–G before XD–E). Half the subjects received the J–B test before the more complex XF–G test (Condition A), while for the other subjects, this testing order was reversed (Condition B). Finally, all subjects received additional tests in which they were given the opportunity to demonstrate the discriminative properties of the J and X stimuli (J1+/J2−, X1+/X2−), and to match the A, J, and X stimuli with newly introduced stimuli of same discriminative properties (e.g. J1-POLITE, J2-RUDE). Experiment 2 was the same except that the training order was reversed (J–A before A1+/A2−, B1+/B2−, and XD–E before D–E, F–G). The results were affected by the training order but not by the testing order. Transfer of discriminative functions and generalized matching on the basis of same functions only occurred reliably when the lower-order contingency tasks were trained first. A stimulus-control account of the data is offered.     

REVIEWS

Books reviewed in this article:
Plant Surfaces. By B. E. J uniper and C. E. J effree .
Agricultural Insect Pests of the Tropics and their Control. By D. S. H ill .
Flora of Connemara and the Burren. By D. A. W ebb and M ary J. P. S cannell .     

Book Reviews

Abstract: Book reviewed in this article:
Nutrition and the Brain , Vol. 8 by R. J. Wurtman and J. J. Wurtman.
Glossary of Biochemistry and Molecular Biology by D. M. Glick.
Antiepileptic Drugs (Third Edition) edited by R. H. Levy, F. E. Dreifuss, R. H. Mattson, B. S. Meldrum, and J. K. Penry.
Developmental Neurobiology (Nestlé Nutrition Workshop Series, Vol. 12) edited by P. Evrard and A. Minkowski.
Allosteric Modulation of Amino Acid Receptors: Therapeutic Implications (Fidia Research Foundation Symposium Series, Vol. 1) by E. A. Barnard and E. Costa.
Neurochemical Pharmacology—A Tribute to B. B. Brodie (Fidia Research Foundation Symposium Series, Vol. 2) edited by E. Costa.
Neuromethods Vol. 11: Carbohydrate and Energy Metabolism edited by A. A. Boulton, G. B. Baker, and R. F. Butterworth.
Adenosine and Adenosine Receptors edited by M. Williams.
Laughing Death: The Untold Story of Kuru by V. Zigas.
Psychoactive Drugs: Tolerance and Sensitization edited by A. J. Goudie and M. W. Emmett-Oglesby.
The NMDA Receptor edited by J. C. Watkins and G. L. Collingridge.
Basic and Clinical Aspects of Neuroscience (Vol. 3): The Role of Brain Dopamine edited by E. Flückiger, E. E. Müller, and M. O. Thorner.
Glycine Neurotransmission edited by O. P. Otterson and J. Storm-Mathisen.
The Neuropeptide Cholecystokinin (CCK): Anatomy and Biochemistry, Receptors, Pharmacology and Physiology edited by J. Hughes, G. Dockray, and G. Woodruff.