Leaf movement of bush bean: a biometeorological perspective

 Leaf movements of bush bean plants were studied at the relatively low photon flux density of 0.2 mmol/m² per s, and air temperatures of 25° and 35° C in a growth chamber. A beta-ray gauge system was used to monitor continuously pulvinus water status and bending. Leaf angles were below the horizontal and were linearly related to the soil water content (R≥−0.91 at 25° C and R≥−0.93 at 35° C). The beta-ray transmission maxima coincided with the stem temperature minima in darkness and vice versa when brightness prevailed as the growth chamber temperature varied with the photoperiod. Leaf angle increased linearly with increased beta-ray transmission. The Q₁₀ temperature coefficient, a measure of the metabolic energy requirement for leaf movement between 25° and 35° C was estimated at 1.8, and the corresponding mean Arrhenius constant at 423 kJ/mol for bush bean. Received: 19 July 1996 / Accepted: 9 September 1996     

A Small Infrared Thermometer for Measuring Leaf Temperature in Leaf Chambers

A small, inexpensive infrared thermometer is described. Thisinstrument is easily used and is more accurate than thermocouplesfor leaf temperature measurements. Errors are estimated to beless than 0.2 °C when measuring leaf temperatures in a typicalleaf chamber. Key words: Infrared thermometer, Leaf temperature, Leaf chamber     

Leaf Temperatures in a Gas Exchange Chamber and in the Open Air

Leaf temperatures in a Koch fully climatized gas-exchange chamberas designed by Siemens and in a similarly equipped open-airreference were measured with horizontally and vertically insertedthermocouples on Nerium oleander L. On a sunny day with onlylittle air movement and an average air temperature of 20.4 °C,leaf over-temperatures in the gas-exchange chamber were loweron average by 2.2 K. The extent of reduction of over-temperaturein the chamber is determined by the reduced global radiationin the chamber and the differences of wind velocities in chamberand reference. Differences in the ventilation intensity in thechamber have no demonstrable influence on the leaf over-temperatures.The over-temperatures of the reference leaves, on the otherhand, depend to a large degree on air velocity. The changedradiation and air flow conditions in the chamber as comparedwith open-air conditions have consequences for the physiologicalreactions of the enclosed plant and must be taken into accountwhen comparing results from gas-exchange measurements with open-airconditions. For further improvements of gas-exchange measurementequipment, air flow conditions and radiation quantity and qualitymight be starting points     

The Temperatures of Leaves in Assimilation Chambers, and in the Open

The temperatures of apple leaves in assimilation chambers wereup to 17° C above the temperature of the outside air insunlight. Except when in deep shade, enclosed leaves had temperatureshigher than ambient. Leaves in the open in the sun were often2–3° C above ambient, but the greatest differencemeasured was 4.3° C. In shade, leaves in the open were asmuch as 0.8° C below the air temperature. Laboratory experimentswith an incandescent lamp showed that the temperature differencebetween an enclosed leaf and the outside air increased linearlywith increasing light intensity above a certain value. Belowthis value it is believed that changes in leaf permeabilitywere sufficiently large to affect the rate of transpirationand therefore the leaf excess temperature-light intensity relationship.Under field conditions leaves may not be in a steady state;this gives rise to more variable measurements which may indicatea non-linear relation between leaf excess temperature and lightintensity. Methods of cooling leaves in chambers were examined.Impractically high rates of flow of air at the ambient temperatureare necessary to reduce the temperature of enclosed leaves appreciably.Some reduction of the leaf excess temperature can be obtainedby filtering the infra-red from the incident light, or by usinga chamber made of material which transmits far infra-red, thoughcondensation reduces the effectiveness of the latter measure.Leaves exhibit rapid changes in temperature, so the heatingproblem cannot be circumvented by brief enclosure. The mosteffective of the techniques examined is to use a water-cooledchamber, though the temperatures of the leaf and water differby several degrees centigrade in bright light. A simple solutionto the heating problem for field assimilation measurements hasnot been found.     

Effects of Temperature and Photoperiod on Winged Beans [Psophocarpus tetragonolobus (L.) D.C.]

The effects of temperature and photoperiod on winged beans werestudied using 15 University of New Guinea (UPS) selections andfive Sri Lanka (SL) selections. They were grown at 25/20 or30/25 °C day/ night temperature at 11 or 14 h photoperiodwith 12 h thermoperiod. Differences in stomatal density wereobserved among selections and between photoperiods. Higher densitiesoccurred at 14 h photoperiod than at 11 h photoperiod. Whenstomatal density was high due to a photoperiod or temperatureeffect, there was a corresponding increase in leaf area andd. wt of plants. Total chlorophyll content at 25/20 °C was higher at 11 hphotoperiod than at 14 h photoperiod in all selections whilethe total chlorophyll content at 30/25 °C varied with thephotoperiod and selection. Leaf area of SL selections was greater than that of UPS selections.Also greater leaf area was observed at 14 h photoperiod thanat 11 h photoperiod, irrespective of the growing temperature. Temperature was as important as photoperiod in controlling floweringof winged beans. All the UPS selections and two SL selectionsflowered at 11 h photoperiod at 25/20 °C but failed to flowerat the same photoperiod at 30/25 °C indicating an interactionbetween temperature and photoperiod. It is likely that wingedbeans have a narrow photoperiodic range, particularly the SLselections. Psophocarpus tetragonolobus (L.) D.C., winged bean, stomatal density, leaf area, flowering, temperature, photoperiod     

Temperature Effects on Phenological Development and Yield of Muskmelon

Our goal was to construct a simple muskmelon phenology modelthat could be run with easily obtainable weather station dataand used by growers to quantify phenological development andaid in projecting harvest dates. A growth chamber experimentwas conducted with two cultivars of muskmelon (‘Gold Rush’and ‘Mission’) to determine how main vine leaf appearancerates responded to temperature. We identified three cardinaltemperatures for leaf appearance rate: the base temperature(10 °C) at which leaf appearance rate was zero; an optimumtemperature (34 °C) at which the rate of leaf appearancewas maximal; and an upper threshold temperature (45 °C)at which leaf appearance rate returned to zero. Using thesethree cardinal temperatures, we constructed a simplified thermalunit accumulator for hourly measurements of air temperature.Main vine plastochron interval (PI), thermal time to harvest,and final yield were determined for three cultivars of muskmelon(‘Explorer’, ‘Gold Rush’ and ‘Mission’)grown in the field at Overton, TX, USA, over six transplantingdates from March to June 1998. PI was calculated for each cultivarx transplanting date combination as the reciprocal of the slopeof main vine node number vs. accumulated hourly thermal units(     

Leaf Extension in Zea mays: II. LEAF EXTENSION IN RESPONSE TO INDEPENDENT VARIATION OF THE TEMPERATURE OF THE APICAL MERISTEM, OF THE AIR AROUND THE LEAVES, AND OF THE ROOT-ZONE

The temperatures of the roots, the apical meristem, and theshoots of Zea mays plants were varied independently of eachother and the rates of leaf extension were measured. When thetemperature of the apical meristem and region of cell expansionat the base of the leaf was kept at 25 °C, changes of leafextension in response to changes of root and shoot temperatureswere less pronounced. When the temperature of the meristematicregion was changed by increments of 5 or 10 °C from 0 to40 °C, and the root and shoot temperatures were kept at25 °C, rapid changes in leaf extension occurred. It was concluded that the rates of leaf extension were controlledat root-zone temperatures of 5 to 35 °C by heating or coolingof the meristematic region. Changes in rates of leaf extensionin response to changes in air temperature were attributed todirect effects on the temperature of the meristematic regionand on the physiology of the leaf.     

Field Studies of Cereal Leaf Growth: I. INITIATION AND EXPANSION IN RELATION TO TEMPERATURE AND ONTOGENY

Measurements of leaf initiation, appearance, and expansion arepresented for winter wheat and spring barley crops. For winterwheat, these processes occurred during periods of several weekswhen fluctuating temperatures influenced process rates. Analysisof these measurements was facilitated by plotting variablesagainst the time integral of temperature above an appropriatebase temperature (O °C), here called thermal time with unitsof °C d. Leaf primordial number and appearance stage increasedlinearly with thermal time for both winter wheat and springbarley which initiated 12 and 9 leaves respectively. When plottedagainst thermal time 90% of laminar and leaf length growth and80% of laminar width growth was satisfactorily described bya straight line for both species. This enabled an average extensionrate and duration of linear growth to be defined for each leaf.When expressed in thermal time, wheat leaves had a similar durationof linear growth (210 °C d; s.d. 30 °C d) with insolationexerting a negligible influence. The first seven barley leaveshad a shorter duration of linear growth (151 °C d; s.d.8 °C d). For wheat, final leaf length and laminar widthincreased with leaf number and were not apparently associatedwith changes in apical development stage. Changes of barleyleaf dimensions with leaf number were more complex.     

Differences in leaf thermoregulation and water use strategies between three co‐occurring Atlantic forest tree species

Given anticipated climate changes, it is crucial to understand controls on leaf temperatures including variation between species in diverse ecosystems. In the first study of leaf energy balance in tropical montane forests, we observed current leaf temperature patterns on 3 tree species in the Atlantic forest, Brazil, over a 10‐day period and assessed whether and why patterns may vary among species. We found large leaf‐to‐air temperature differences (maximum 18.3 °C) and high leaf temperatures (over 35 °C) despite much lower air temperatures (maximum 22 °C). Leaf‐to‐air temperature differences were influenced strongly by radiation, whereas leaf temperatures were also influenced by air temperature. Leaf energy balance modelling informed by our measurements showed that observed differences in leaf temperature between 2 species were due to variation in leaf width and stomatal conductance. The results suggest a trade‐off between water use and leaf thermoregulation; Miconia cabussu has more conservative water use compared with Alchornea triplinervia due to lower transpiration under high vapour pressure deficit, with the consequence of higher leaf temperatures under thermal stress conditions. We highlight the importance of leaf functional traits for leaf thermoregulation and also note that the high radiation levels that occur in montane forests may exacerbate the threat from increasing air temperatures.     

The Growth and Development of Maize (Zea mays L.) at Five Temperatures

The objectives of this work were to measure growth and developmentrates over a range of temperatures and to identify processeswhich may limit vegetative yield of maize (Zea mays L.). Twosingle cross Corn Belt Dent maize hybrids were grown from sowingin a diurnal temperature regime of 16/6 °C day/night andin constant temperature environments of 16, 20, 24 and 28 °C.The 16/6 °C environment was close to the minimum for sustainedgrowth and 28 °C was near the optimum. Entire plants wereharvested at stages with 4, 6, 7 and 8 mature leaves in alltemperature treatments except 20 °C in which the final twoharvests were carried out at 9 and 10 mature leaves. Mean totalleaf number varied between 19.5 and 16.0 with the maximum occurringat 16/6 °C. Although harvests were carried out at comparableleaf numbers, and hence at similar developmental stages, thetime interval between sowing and harvest decreased considerablyas temperatures increased. The relative rates of dry weight and leaf area accumulationwith time increased with a Q₁₀ of 2.4 between 16 and 28 °C,while leaf appearance rate increased with a Q₁₀ of 2.9 overthe same range; both rates were highest at 28 °C. Althoughdry matter partitioning to the shoots increased with temperature,the area of individual leaves varied in a systematic patternwhich resulted in maximum leaf area, leaf area duration andconsequently dry weight being realized at 20 °C for anygiven stage of development. Zea mays, corn, low temperature stress, temperature response, growth, development     

Leaf Extension in Zea mays: I. LEAF EXTENSION AND WATER POTENTIAL IN RELATION TO ROOT-ZONE AND AIR TEMPERATURES

The temperature of the roots and shoots of Zea mays plants werevaried independently of each other and the rates of leaf extensionand leaf water potentials were measured. Restrictions of leafextension occurred when root temperatures were lowered from35 to 0 °C, but leaf water potentials were lowered onlyat root temperatures below 5 °C. Similar changes in ratesof leaf extension were measured at air temperatures from 30to 5 °. Between 30 and 35 °C air temperature, in anunsaturated atmosphere, restrictions of leaf extension wereassociated with low leaf water potentials. It was concluded that, at root temperatures 5 to 35 °C,and shoot temperatures 5 to 30 °C, water stress was notthe main factor restricting the extension of Zea mays leaves.     

The effect of root temperature on rose plants in relation to air temperature

Rose plants (Rosa hybrida ‘Sonia’=‘Sweet Promise’) were grown in heated (minimum night temperature 17°C), and unheated greenhouses with or without root heating to 21°C. These trials covered 6 growth cycles extending over two winter seasons. In the heated greenhouse, root heating did not increase yield, flower quality or plant development. In the unheated greenhouse, root-heated plants grew as well as those in the air-heated greenhouse as long as the air temperature did not fall below 6°C. When minimum night temperatures fell below 6°C, growth, yield and quality were reduced, irrespective of root temperature. Daytime plant water relations were studied in plants growing at 6 different root temperatures in the unheated greenhouse. Leaf resistance to water diffusion was lowest at optimal root temperature. Total leaf water potential was not significantly affected by root temperature.     

Apparatus for the Simultaneous Measurement of Water Vapour and Carbon Dioxide Exchanges of Single Leaves

Equipment is described which delivers air with concentrationsof CO₂ and water vapour closely controlled in the ranges 0 to2500 ppm and 5 to 15 mb respectively, at flow rates of up to10 1 min^-1, to each four leaf chambers. The leaf temperatureis controlled to ±0.5 °C and, with a light intensityof 0.3 cal cm^-2 min^-1 visible radiation (0.4 to 0.7 µm)leaf temperature can be maintained at 17.5 °C.The apparatusused to measure the concentration differences between the watervapour and CO₂ entering and leaving the leaf chamber (used tocalculate transpiration, photosynthetic, and respiration rates)is described in detail.Results of tests, which show the necessityfor mounting a fan within the leaf chamber, are reported.Typicallight- and CO₂-response curves are given for kale leaves (Brassicaoleracca var. acephala) and an attempt is made to quantify theerrors in the measurement of photosynthesis and transpiration.     

Bean Leaf Expansion in Relation to Temperature

When dwarf Phaseolus vulgaris plants were grown in a controlledenvironment at 20, 25, 30, and 35° C, expansion of the primaryleaves occurred in two phases with an intermediate lag. Varyingrates and duration of expansion were involved, leading to greatestfinal areas at the two intermediate temperatures. Dry weightsof the leaves and leaf areas were similary influenced by temperature,except that the initial rates of increase continued for a longerperiod for weights than for areas. The rates of cell divisionand final numbers of cells were similar from 25 to 35° C,but both were decreased at 20° C. Final cell sizes were,on the other hand, decreased only at the highest temperature.The time trends of cell expansion varied greatly with temperature. Leaf expansion is discussed as a possible consequence of substratesupply, which may be determined by temperature in a number ofways. Cell division and cell expansion are not considered tobe joint direct determinants of leaf expansion. Temperatureinfluences division, with two consequences; the rate interactswith substrate supply to determine size of cells, and finalcell number affects potential leaf area. Cell size is regardedas being secondary to numbers of cells and total material available,although some factors can vary cell size independently of substrate,e.g. water status. An important control of leaf growth, until the attainment ofabout half the final area, may be exercised by way of the leaf.Subsequently, intra-plant competition is likely to dominate.     

Temperature Response of Vernalization in Wheat: A Developmental Analysis

The vernalization response of wheat ( Triticum aestivum L.)was reinterpreted from a developmental perspective, using currentconcepts of the developmental regulation of wheat morphologyand phenology. At temperatures above 0 °C, the effects ofthe process of vernalization per se in wheat are confoundedby the effects of concurrent vegetative development. These effectsare manifested by differences in the number of leaves initiatedby the shoot apex prior to floral initiation, which in turnaffects the subsequent rate of development to ear emergenceand anthesis. Leaf primordia development during vernalizationand total leaf number at flowering were used to develop criteriato define both the progress and the point of saturation of thevernalization response. These criteria were then used to reinterpretthe results of Chujo ( Proceedings of the Crop Science Societyof Japan 35 : 177–186, 1966), and derive the temperatureresponse of vernalization per se for plants grown under saturatinglong day conditions. The rate of vernalization increased linearlywith temperature between 1 and 11 °C, such that the timetaken to saturate the vernalization response decreased from70 d at 1 °C to 40 d at 11 °C. The rate declined againat temperatures above 11 °C, and 18 °C was apparentlyineffective for vernalization. Total leaf number at saturation,however, increased consistently with temperature, as a resultof the balance between the concurrent processes of leaf primordiuminitiation and vernalization. Total leaf number at saturationincreased from 6 at 1 °C to 13.3 at 15 °C, which inturn influenced the time taken to reach ear emergence. The advantagesof using this developmental interpretation of vernalizationas the basis for a mechanistic model of the vernalization responsein wheat are discussed. Triticum aestivum L.; wheat; vernalization; rate; temperature; primordia; leaf number; flowering     

Changes in the Soluble Protein and Free Amino Acid Content of Chill-Sensitive and Chill-Resistant Plants During Chilling and Hardening Treatments

The effects of low temperature (5 °C and 12°C) and droughttreatments on leaf soluble protein content and free amino acidcontent have been investigated in four species, which were rankedaccording to chilling-sensitivity: pea (chill-resistant), mungbean (highly chill-sensitive), and tomato and french bean (intermediatechilling-sensitivity). Drought treatment caused a 30–40% decrease in proteinlevels, and in all but the mung bean, a 100–200% increasein free amino acid concentration. Four days chilling at 5°C,85% r.h. caused leaf water content to decrease by almost 50%in the mung bean, but by only approximately 6–7% in theother three species. During this treatment the leaf solubleprotein content decreased in all four species although the decreasewas greatest and most rapid in the mung bean, commencing with8 h of chilling (coinciding closely with the onset of waterloss), and decreasing by over 80% after 4 d. In the chill-sensitivespecies (but not in the pea) the decrease in protein contentwas accompanied by an increase in free amino acid content. However,on a mgg^–1 dry wt. basis, this increase was insufficientto account for all the protein lost. When plants of each specieswere chilled at 5°C, 100% r.h., water loss was greatly reducedor prevented and there was no significant decrease in leaf solubleprotein. It is concluded that the protein decrease which occurredat 5°C, 85% r.h., was a response to water loss and not thedirect result of low temperature. However, chilling at 100%r.h. did cause an increase in free amino acid content of thechill-sensitive species, suggesting that this was a direct responseto low temperature. Although drought treatment caused a 6–20 fold increasein free proline content in the leaves of the four species examined,chilling (5°C) and chill-hardening (12°C) caused littlechange in free proline content, indicating that the accumulationof this ‘protective’ amino acid is unlikely to contributeto the effectiveness of the chill-hardening treatment. Key words: Low Temperature, Drought, Leaf soluble protein.content, Amino acids     

Pulvinus activity,leaf movement and leaf water-use efficiency of bush bean (<Emphasis Type="Italic">Phaseplus vulgaris</Emphasis> L.) in a hot environment

Pulvinus activity of Phaseolus species in response to environmental stimuli plays an essential role in heliotropic leaf movement. The aims of this study were to monitor the continuous daily pulvinus movement and pulvinus temperature, and to evaluate the effects of leaf movements, on a hot day, on instantaneous leaf water-use efficiency (WUE_i), leaf gas exchange, and leaf temperature. Potted plants of Phaseolus vulgaris L. var. Provider were grown in Chicot sandy loam soil under well-watered conditions in a greenhouse. When the second trifoliate leaf was completely extended, one plant was selected to measure pulvinus movement using a beta-ray gauging (BRG) meter with a point source of thallium-204 (²⁰⁴Tl). Leaf gas exchange measurements took place on similar leaflets of three plants at an air temperature interval of 33–42°C by a steady-state LI-6200 photosynthesis system. A copper-constantan thermocouple was used to monitor pulvinus temperature. Pulvinus bending followed the daily diurnal rhythm. Significant correlations were found between the leaf-incident angle and the stomatal conductance (R ² = 0.54; P < 0.01), and photosynthesis rate (R ² = 0.84; P < 0.01). With a reduction in leaf-incidence angle and increase in air temperature, WUE_i was reduced. During the measurements, leaf temperature remained below air temperature and was a significant function of air temperature (r = 0.92; P < 0.01). In conclusion, pulvinus bending followed both light intensity and air temperature and influenced leaf gas exchange.     

Influence of Temperature on Potentiation of Cellulysin-Induced Ethylene Biosynthesis by Ethylene

The role temperature plays during ethylene pretreatment on subsequentinduction of ethylene biosynthesis by Cellulysin or by a partiallypurified ethylene inducing factor (EIF) from Cellulysin in tobacco(Nicotiana tabacum L., cv. Xanthi) leaf discs was studied. Leaveswere pretreated with ethylene at three temperatures (7°C,25°C, and 40°C) followed by the induction of ethylenebiosynthesis at 23°C. At 25°C, ethylene pretreatmentstimulated subsequent Cellulysin-, EIF- or ACC-induced ethylenebiosynthesis and conjugation of ACC. At 7°C ethylene pretreatmenthad little effect on subsequent Cellulysin-, EIF- or ACC-inducedethylene biosynthesis, while 40°C pretreatment inhibitedsubsequent Cellulysin-, EIF- or ACC-induced ethylene biosynthesisat 23°C. The high temperature (40°C) pretreatment inhibitedsubsequent conversion of ACC to ethylene in both air and ethylenetreated tissues. (Received May 20, 1988; Accepted January 24, 1989)     

Diurnal Changes in Citrus Leaf Thickness, Leaf Water Potential and Leaf to Air Temperature Difference

Diurnal changes in leaf water potential and leaf thickness ofwell-watered citrus trees were found to be highly correlated.Midday decreases in leaf thickness of about 30–35 µm reflected midday decreases in leaf water potential of about1.1–1.3 MPa from predawn values. Leaf water potentialwas also correlated with changes in leaf-to-air temperaturedifference and ambient vapour pressure deficit. Leaf thicknessas well as leaf to air temperature difference could possiblybe used to monitor leaf water status continuously as an indicatorof citrus tree water stress.