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1.
《Cell biology international reports》1981,5(6):575-579
The microfilaments of cellular slime mold cells are known to be dislocated from their attachment to the plasma membrane by 10% dimethyl sulfoxide (DMSO). We report here that 10% DMSO inhibited capping of lentil lectin receptors on the surface of vegetative cells of the cellular slime mold, Dictyostelium mucoroides When the DMSO was washed from the cells, capping took place. Capping of surface immunoglobulin of mouse lymphocytes was also reversibly inhibited by 10% DMSO. 相似文献
2.
Aip1p cooperates with actin-depolymerizing factor (ADF)/cofilin to disassemble actin filaments in vitro and in vivo, and is proposed to cap actin filament barbed ends. We address the synergies between Aip1p and the capping protein heterodimer Acp1p/Acp2p during clathrin-mediated endocytosis in fission yeast. Using quantitative microscopy and new methods we have developed for data alignment and analysis, we show that heterodimeric capping protein can replace Aip1p, but Aip1p cannot replace capping protein in endocytic patches. Our quantitative analysis reveals that the actin meshwork is organized radially and is compacted by the cross-linker fimbrin before the endocytic vesicle is released from the plasma membrane. Capping protein and Aip1p help maintain the high density of actin filaments in meshwork by keeping actin filaments close enough for cross-linking. Our experiments also reveal new cellular functions for Acp1p and Acp2p independent of their capping activity. We identified two independent pathways that control polarization of endocytic sites, one depending on acp2+ and aip1+ during interphase and the other independent of acp1+, acp2+, and aip1+ during mitosis. 相似文献
3.
A pH-sensitive electrode was applied to measure activity of H+ ions in the medium surrounding excitable cells of pumpkin (Cucurbita pepo L.) seedlings during cooling-induced generation of action potential (AP). Reversible alkalization shifts were found to occur synchronously with AP, which could be due to the influx of H+ ions from external medium into excitable cells. Ethacrynic acid (an anion channel blocker) reduced the AP amplitude but had no effect on the transient alkalization of the medium. An inhibitor of plasma membrane H+-ATPase, N,N’-dicyclohexylcarbodiimide suppressed both the AP amplitude and the extent of alkalization. In experiments with plasma membrane vesicles, the hydrolytic H+-ATPase activity was subjected to inhibition by Ca2+ concentrations in the range characteristic of cytosolic changes during AP generation. The addition of a calcium channel blocker verapamil and a chelating agent EGTA to inhibit Ca2+ influx from the medium eliminated the AP spike and diminished reversible alkalization of the external solution. An inhibitor of protein kinase, H-7 alleviated the inhibitory effect of Ca2+ on hydrolytic H+-ATPase activity in plasma membrane vesicles and suppressed the reversible alkalization of the medium during AP generation. The results provide evidence that the depolarization phase of AP is associated not only with activation of chloride channels and Cl? efflux but also with temporary suppression of plasma membrane H+-ATPase manifested as H+ influx. The Ca2+-induced inhibition of the plasma membrane H+-ATPase is supposedly mediated by protein kinases. 相似文献
4.
A Staroselsky L Peleg E Levitov H Slor N S Kosower 《Cell biology international reports》1986,10(2):121-129
Concanavalin A capping was studied in immunoglobulin-secreting hybridomas derived from fusion of mouse myeloma NSO cells with mouse spleen lymphocytes. The cells of the parental populations differed significantly in capping ability (low in myeloma cells and high in the lymphocytes). Among the hybridoma cells tested, several clones showed low capping, similar to that of the myeloma cells, some showed a good degree of capping, similar to that of the lymphocytes and other clones expressed an intermediate capping response. Capping was significantly increased in hybridoma clones of intermediate capping ability following in vivo intraperitoneal growth. A possible relationship of the variation in capping response to cell motility and to metastatic capacity is pointed out. 相似文献
5.
Redistribution (capping) of normal and tumor-associated surface antigens was studied on murine and human cells by the indirect membrane immunofluorescence (MIF) technique. The capping of H-2 isoantigens was compared on normal mouse T-lymphocytes and on YAC cells, a Moloney leukemia virus (MLV) induced lymphoma. H-2 and Moloney virus induced cell surface antigen (MCSA) capping was compared on three YAC lines with different MCSA concentrations. H-2 and tumor-associated surface antigen capping was compared on two polyoma induced sarcoma lines and five methylcholanthrene induced sarcoma lines. In the human system, IgM-capping was compared on normal lymphocytes and on the Burkitt lymphoma derived Daudi line. Capping of HL-A and the Epstein-Barr virus (EBV) determined membrane antigen (MA) was compared on the Burkitt lymphoma derived line Maku and on EBV-superinfected Daudi cells. H-2 antigens on normal murine cells capped more promptly and on a larger fraction of the cell population on the various tumor cells. Surface associated IgM showed a better capping on normal lymphocytes than on Daudi cells. All tumor associated antigens except MCSA, showed good capping. MCSA was almost completely refractory to capping. Increasing concentrations of MCSA appeared to inhibit the capping of H-2 on the YAC sublines with different concentrations of MCSA. The polyoma induced ascites sarcoma (SEWA) did not cap either with regard to H-2 or the polyoma determined surface antigen. 相似文献
6.
Human lymphocytes at 0°C in low Na+ medium accumulate both K+ and Na+ to levels higher than in the external medium. This is not due to an impermeable compartment or a Donnan equilibrium, and is incompatible with the membrane Na+-pump concept. In contrast, it supports prior evidence that ion exchange in lymphocytes is mediated by adsorption onto and desorption from fixed anionic sites within the cell. Additional aspects of ion and water contents of cells in low Na+ medium are described and are explained by this concept. 相似文献
7.
The cell membrane potential of cultured Chinese hamster cells is known to increase at the start of the S phase. The putative role of the cell membrane potential as a regulator of cell proliferation was examined by following the cell cycle traverse of synchronized Chinese hamster cells in the presence or absense of high exogenous levels of potassium. An increase in external potassium levels results in a depressed membrane potential and a reduced rate of cell proliferation. A potassium concentration of 115 mM was used in experiments with synchronized cells since at that level cell proliferation is almost completely halted, recovery of growth is rapid and complete, and the membrane potential is reduced to a level well below that normally found in cells in the G1 phase. A mitotic population was divided into four aliquots and plated in either control medium or medium containing 115 mM K+. Cells placed directly into high K+ medium were retarded in their exit from mitosis and displayed a delayed and abnormal entry into the S phase. If control medium was added after two hours, cell cycle traverse was normal, but delayed by two hours compared to control cells. If the mitotic cells were plated directly into control medium and two hours later were shifted to high K+ medium, the cells entered the S phase in the absence of the normally observed increase in membrane potential and proceeded to the next mitosis normally. It was concluded that the increase in membrane potential observed at the start of the S phase in isolated synchronized cells is not a requirement for the initiation of DNA synthesis. In addition, sensitivity to the high potassium regimen was found at two different times during the cell cycle. In one case, cells were impeded in their transit through mitosis. Such cells displayed an altered chromosome structure which may account for the partial mitotic block. In the second case, synchronized cells displayed a sensitivity to the high potassium regimen in early G1 which appeared to be separate from the block in mitosis and independent of a change in the membrane potential. 相似文献
8.
The Na+ uptake into neuroblastoma x glioma hybrid cells was measured in Hepes-buffered EMEM containing 10% calf serum and 5 mM ouabain in the presence and absence of amiloride (1.0 mM). Amiloride was found to markedly inhibit net Na+ influx (by approximately 50%). Examination of the effect of amiloride on net Na+ influx in the absence of calf serum revealed that a significant amiloride-sensitive Na+ influx remains even under serum-deprived conditions, although the degree of amiloride inhibition (35%) is substantially lower than that found in the presence of serum. The amiloride-insensitive portion of Na+ influx was found to be independent of serum effects. Estimation of resting membrane potential was made by measurement of the steady state distribution of the lipophilic cation, TPP+, in the presence and absence of amiloride. A large, immediate increase in TPP+ uptake, indicative of a membrane hyperpolarization, was seen upon addition of amiloride. Determination of the effect of amiloride on resting membrane potential of serum-deprived cells showed that cells are hyperpolarized to a greater extent in the presence than in the absense of amiloride, and that serum exerts a depolarizing effect on the cells. Thus, serum-stimulation of Na+ influx results in a depolarization of resting membrane potential, while amiloride inhibition of Na+ influx causes a hyperpolarization. These data strongly suggest that NG108-15 cells possess an electrogenic Na+ influx pathway that is sensitive to amiloride inhibition and enhanced by serum. 相似文献
9.
Harrihar A. Pershadsingh Evan B. Stubbs Jr. William D. NoteboomMarie L. Vorbeck Arlene P. Martin 《生物化学与生物物理学报:生物膜》1985,821(3)
The involvement of Ca2+-activated K+ channels in the regulation of the plasma membrane potential and electrogenic uptake of glycine in SP 2/0-AG14 lymphocytes was investigated using the potentiometric indicator 3,3′-diethylthiodicarbocyanine iodide. The resting membrane potential was estimated to be −57 ± 6 mV (n = 4), a value similar to that of normal lymphocytes. The magnitude of the membrane potential and the electrogenic uptake of glycine were dependent on the extracellular K+ concentration, [K+]o, and were significantly enhanced by exogenous calcium. The apparent Vmax of Na+-dependent glycine uptake was doubled in the presence of calcium, whereas the K0.5 was not affected. Ouabain had no influence on the membrane potential under the conditions employed. Additional criteria used to demonstrate the presence of Ca2+-activated K+ channels included the following: (1) addition of EGTA to calcium supplemented cells elicited a rapid depolarization of the membrane potential that was dependent on [K+]o; (2) the calmodulin antagonist, trifluoperazine, depolarized the membrane potential in a dose-dependent and saturable manner with an IC50 of 9.4 μM; and (3) cells treated with the Ca2+-activated K+ channel antagonist, quinine, demonstrated an elevated membrane potential and depressed electrogenic glycine uptake. Results from the present study provide evidence for Ca2+-activated K+ channels in SP 2/0-AG14 lymphocytes, and that their involvement regulates the plasma membrane potential and thereby the electrogenic uptake of Na+-dependent amino acids. 相似文献
10.
NAD+ levels in resting human lymphocytes obtained from 20 donors were found to be 69.9 ± 21.7 pmols/106 cells. After 3 days of phytohemagglutinin (PHA) stimulation the NAD+ levels rose to 452 ± 198 pmols/106 cells. NADH, NADP+ and NADPH also increased in mitogen-stimulated lymphocytes, but the major portion of the increase in total pyridine nucleotide pools was accounted for by the increase in NAD+. When PHA-stimulated lymphocytes were incubated in nicotinamide-deficient growth medium, there was no significant increase in their total pyridine nucleotide pools; however, the ratios of oxidized to reduced pyridine nucleotides changed in a similar fashion to cells grown in medium containing nicotinamide. When lymphocytes in nicotinamide-deficient medium were stimulated with PHA they increased their levels of DNA synthesis and cell replication in a similar fashion to cells growing in nicotinamide-supplemented media. Human lymphocytes were able to synthesize pyridine nucleotides from nicotinamide or nicotinic acid; however, in the absence of a preformed pyridine ring they did not efficiently use tryptophan for the synthesis of NAD. Uptake of [carbonyl-14C]nicotinamide and conversion to NAD was markedly increased in PHA-stimulated lymphocytes; these cells also showed a marked increase in activity of the enzyme adenosine-triphosphate-nicotinamide mononucleotide (ATP-NMN) adenylyl transferase. 相似文献
11.
12.
Reino Heikkil Tore Godal Aase Henriksen Jens-Gustav Iversen 《Experimental cell research》1981,136(2):447-454
F(ab′)2 anti-μ, as well as anti-δ and anti-γ heavy chains have been found to induce strong increases in the influx of 86Rb+ (a potassium analogue) in cell suspensions from a proportion of human lymphomas staining monoclonally for B cells. The specificity of the response corresponded to the surface immunoglobulin (sIg) isotype present on lymphoma cells. Even when carried out under closely analogous conditions, no relationship between capping and 86Rb+ influx was observed. On the other hand, an association between 86Rb+ influx and mitogenic response to anti-immunoglobulin (anti-Ig) was observed with concordant results in 19 out of 21 comparisons made. These findings suggest that 86Rb+ influx, which appears to be associated with mitogenic signals, and capping may arise as independent phenomena even when elicited through the same membrane receptor (sIg). 相似文献
13.
Christel Devaud Benoît Rousseau Sonia Netzer Vincent Pitard Christian Paroissin Camille Khairallah Pierre Costet Jean-François Moreau Franck Couillaud Julie Dechanet-Merville Myriam Capone 《Cancer immunology, immunotherapy : CII》2013,62(7):1199-1210
The role of human intraepithelial Vδ1+ γδ T cell cytotoxic effectors in the immune surveillance against metastatic colon cancer has never been addressed, despite their reported capacity to infiltrate colon carcinomas and to kill colonic cancer cells in vitro. We previously showed that Vδ1+ γδ T cells are enriched in blood in response to cytomegalovirus (CMV) infection, and that such increase may be protective against epithelial cancers. The objective of the present study was to investigate whether CMV-induced Vδ1+ γδ T lymphocytes could inhibit the propagation of human colon tumors in vivo, in order to evaluate their immunotherapeutic potential in this context. Even though metastases are an important cause of death in various cancers including colorectal cancer (CRC), the anti-metastatic effect of immune effectors has been poorly analyzed. To this purpose, we set up a reliable model of metastatic colon cancer through orthotopic implantation of luciferase-expressing human HT29 cells in immunodeficient mice. Using bioluminescence imaging to follow the outcome of colonic cancer cells, we showed that a systemic treatment with CMV-induced Vδ1+ γδ T cells could not only inhibit primary colon tumor growth but also the emergence of secondary tumor foci in the lungs and liver. Finally, our data lead to propose that Vδ1+ γδ T lymphocytes may directly influence the appearance of metastases independently from their control of primary tumor size. These findings, which extend our previous work, pave the road for the potential manipulation of Vδ1+ γδ T lymphocytes in novel anti-CRC immunotherapeutic protocols. 相似文献
14.
Michihisa Miyake Kenzo Kurihara 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1983,762(2):256-264
In the previous paper, we showed that the K+ channels of the mouse neuroblastoma cell (clone N-18) are closed at low concentration of external K+ ([K+]0) including the physiological concentration for the cells. In the present study, the origin of the resting membrane potential of N-18 cells has been examined. (1) The resting membrane potential of N-18 cells was depolarized by increasing concentration of the polyvalent cations (La3+, Fe3+, Co2+, Ca2+, Sr2+, Mg2+) and by decreasing the pH of the medium. The input membrane resistance was slightly increased during the depolarization. The depolarization was not explained in terms of the diffusion of the cations across the membrane, since the trivalent cations of greater ionic size were effective at much lower concentrations than the divalent cations. The results obtained from the measurements of 86Rb efflux suggested that the depolarization cannot be explained in terms of blocking of the K+ channels by the cations. (2) An increase in Ca2+ concentration from 0.3 to 1.8 mM induced depolarization of about 10 mV at low [K+]0 where the K+ channels are closed, but did not induce any depolarization at high [K+]0 where the channels are open. (3) In order to estimate the changes in the zeta-potential, the electrophoretic mobility of N-18 cells was measured under various conditions. There was a close correlation between the changes in the zeta-potential and those in the membrane potential in response to the polyvalent cations and proton. On the other hand, an increase in K+-concentration in the medium, which induced a large depolarization in the cells, did not affect the zeta-potential. (4) The results obtained were explained by an electrical circuit model for the membranes of N-18 cells. In this model, an electrical circuit for the membrane part carrying no selective ionic channels, in which changes in the surface potential directly affect the transmembrane potential, is connected in parallel to the usual circuit model representing selective ionic channel systems. It was concluded that the surface potential contributes significantly to the resting membrane potential of N-18 cells at low [K+]0 where the K+ channels are closed. 相似文献
15.
Aluminium induced membrane potential (Em) changes and potential changes during repolarization phase of the action potential (AP) in the internodal cells of Nitellopsis obtusa after blocking H+-ATPase activity by DCCD were investigated. Micromolar concentrations of DCCD are sufficient to give complete and irreversible
inhibition of proton pumping. The membrane potential was measured by conventional glass-microelectrode technique. We found
that the half-amplitude pulse duration differs significantly between standard conditions, after DCCD application, and after
H+-ATPase blocking and subsequent Al3+ treatment: 4.9, 7.7 and 17.2 seconds, respectively. We propose that in the short term (2 hours) treatment of Al3+, the decrease in membrane potential was compensated for by H+-ATPase activity. Blocking H+-ATPase activity by DCCD can enhance the influence of Al3+ on the bioelectrical activity of cell membranes. 相似文献
16.
Philip C. Laris Matthew Bootman Harrihar A. Pershadsingh Rose M. Johnstone 《生物化学与生物物理学报:生物膜》1978,512(2):397-414
The membrane potential of the Ehrlich ascites tumor cell was shown to be influenced by its amino acid content and the activity of the Na+: K+ pump. The membrane potential (monitored by the fluorescent dye, 3,3′-dipropylthiodicarbocyanine iodide) varied with the size of the endogenous amino acid pool and with the concentration of accumulated 2-aminoisobutyrate. When cellular amino acid content was high, the cells were hyperpolarized; as the pool declined in size, the cells were depolarized. The hyperpolarization seen with cellular amino acid required cellular Na+ but not cellular ATP. Na+ efflux was more rapid from cells containing 2-aminoisobutyrate than from cells low in internal amino acids. These observations indicate that the hyperpolarization recorded in cells with high cellular amino acid content resulted from the electrogenic co-efflux of Na+ and amino acids.Cellular ATP levels were found to decline rapidly in the presence of the dye and hence the influence of the pump was seen only if glucose was added to the cells. When the cells contained normal Na+ (approx. 30 mM), the Na+: K+ pump was shown to have little effect on the membrane potential (the addition of ouabain had little effect on the potential). When cellular Na+ was raised to 60 mM, the activity of the pump changed the membrane potential from the range ?25 to ?30 mV to ?44 to ?63 mV. This hyperpolarization required external K+ and was inhibited by ouabain. 相似文献
17.
Jack Ferrier Jeff Dixon Alan Illeman Eva Dillon Inka Smith 《Journal of cellular physiology》1982,113(2):267-272
Measurements were made of plasma membrane voltage noise in cells of a bone cell clone. The measurements were made under conditions intended to approximate in vivo conditions more closely than in previous electrical measurements on small mammalian cells. Mononuclucell of normal size, imbedded in a collagen matrix, were used. The electrical state of the cell membrane under normal conditions was characterized by low-frequency random fluctuations (noise) of high magnitude. Hyperpolarizing spikes were observed in some cells. Power spectrum analysis revealed that the random fluctuations were actually a sum of incoherent spike patterns, with spikes of the same time width as those seen in the clearly spiking patterns. This analysis, combined with similar measurements in a high [K+], low [Na+] medium, showed that the fluctuation/spiking phenomenon resulted from modulation of K+ and Na+ transport by a control process at a level higher that that of the individual channels. This process persisted when the membrane potential was depolarized. These results indicate that the membrane potential is not part of the feedback loop producing the fluctuation/spiking phenomenon. 相似文献
18.
Electrical evidence for rhythmic changes in the cotransport of sucrose and hydrogen ions in Samanea pulvini 总被引:2,自引:1,他引:1
Measurements with microelectrodes implanted into Samanea saman (Jacq.) Merrill leaf pulvini showed that membrane potentials were rhythmically sensitive to the application of sucrose. The magnitude of the electrical depolarizations induced by sucrose were dependent on the concentration of H+ in the medium, yet changes in [H+] alone did not greatly affect the potential. During sucrose-induced electrical depolarization, there was a slight increase in the pH of the bathing medium; both effects were abolished by high levels of K+, Na+ or Ca2+ in the medium. These observations indicate that H+ enter the cells by some cooperative action with sucrose. A model of H+-substrate cotransport is proposed in which a sugar carrier in the membrane is made more permeable by the attachment of a proton. The rhythmic nature of this proposed cotransport may be related to circadian leaf-movements in this plant. 相似文献
19.
Christof Scheid Joseph Prendiville Gordon Jayson Derek Crowther Brian Fox George R. Pettit Peter L. Stern 《Cancer immunology, immunotherapy : CII》1994,39(4):223-230
Summary Bryostatin 1 is a protein kinase C activator that inhibits growth of tumour cells and activates lymphocytes in vitro, properties that have encouraged its use in phase 1 clinical studies as an anticancer agent. We investigated interleukin-2(IL-2)-induced proliferation and lymphokine-activated killer (LAK) cell activity in peripheral blood mononuclear cells (PBMC) from cancer patients receiving Bryostatin intravenously. After Bryostatin administration both LAK generation and proliferation were enhanced when patients' PBMC were stimulated with IL-2 in vitro. However, when normal donors' PBMC were cultured in vitro in the presence Bryostatin and IL-2, LAK induction was inhibited while IL-2-driven proliferation was increased. These effects were also seen following only 2 h exposure to Bryostatin and could be elicited by conditioned medium from Bryostatin-pretreated cells. Neither IL-4 nor interferon was detected in the conditioned medium. Bryostatin in vitro was found to increase expression of IL-2 receptors on CD4+, CD8+ and CD56+ cells and augment the proportion of CD8+ cells in conjunction with IL-2. We conclude that Bryostatin in combination with IL-2 in vitro enhances proliferation and IL-2 receptor expression on lymphocytes, favouring CD8+ cells while suppressing the generation of LAK activity. Intravenous administration of Bryostatin increases the potential of IL-2 to induce proliferation and LAK activity in lymphocytes which, taken together with its putative direct antitumour effect, makes Bryostatin an interesting candidate for clinical trials in combination with IL-2.B.F. and P.L.S. are supported by the Cancer Research Campaign 相似文献
20.
Summary Changes in parietal cell membranous structures that accompany the onset of acid secretion were studied with electron microscopy using isolated gastric glands from rabbit. A stereological analysis was performed to quantitate the morphological changes occurring within 5 min following histamine stimulation. These changes were compared to the changes resulting from osmotic expansion of parietal cell components following addition of 1mm aminopyrine (AP) to glands incubated in medium containing 108mm K+ (high-K+). Morphometric analyses, together with measurements of glandular water content, indicated that parietal cells swell in high-K+ medium. Addition of 1mm AP to glands incubated in high-K+ medium resulted in massive distention of the secretory canaliculus but no difference was observed in the amount of tubulovesicular membrane or the relative size of these cytoplasmic structures. In the histamine-treated glands the parietal cells displayed a rapid loss of tubulovesicular membrane and a reciprocal increase in canalicular membrane. These morphological changes were complete long before a maximum level of acid formation was achieved. Taken together, these results indicate that; (i) the morphological change accompanying stimulation does not require acid formationper se; (ii) the site of acid secretion is the intracellular canaliculus and not the tubulovesicles; (iii) there is no preexisting actual or potential continuity between the tubulovesicular space and the canalicular space; and (iv) the AP-induced expansion of the canaliculus in high-K+ medium, while yielding some valuable information, is not an appropriate model for studying the normal stimulus-induced morphological transition, despite a superficial similarity of appearance. 相似文献