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He-Ne激光照射对血液及其组分荧光光谱影响的实验研究 总被引:2,自引:0,他引:2
为研究弱激光照射对人血液携氧能力的影响及机制,我们用荧光仪分别测量了He-Ne激光照射前后正常血液及其组分(血浆、红细胞)的荧光光谱,研究了激光照射导致的光谱变化,并分析了光谱变化与血液携氧能力改变的关系。实验结果显示:全血液标本在490nm及614nm附近有荧光峰值;血浆的荧光则主要分布在420-500nm之间;红细胞在500nm及614nm附近有荧光。He-Ne激光照射后,全血液及红细胞在614nm处的荧光谱都有较明显的变化,且较相似。由此可得出结论,He-Ne激光照射可影响血液的携氧能力。 相似文献
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低强度激光血管内照射治疗的机理初探 总被引:3,自引:1,他引:2
用532nm的激光作为激光光源分别测量正常血液、血浆及HBsAg阳性血液、血浆的荧光光谱。血液光谱的测量结果显示,两种血液樯本都在630nm及710nm附近出现有荧光峰值。从光谱学的角度分析,可得出激光照射血液治疗的内在机制在激光照射可增强血液的携氧能力,从而促进了机体的新陈代谢。血浆图谱测量结果显示,正常血浆及HBsAg阳性血浆在738nm处的荧光强度有显著差异,可作为临床光谱诊断中判断HBsAg阳性的标准和依据。对HBsAg阳性血液的生化检测结果显示,He-Ne激光照射可抑制或杀伤乙肝病毒。 相似文献
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人肌肌酸激酶在SDS溶液中失活与构象变化的比较研究 总被引:3,自引:0,他引:3
应用紫外差吸收光谱、荧光发射光谱、CD先谱等监测手段,研究了SDS溶液滴定人肌肌酸激酶时的构象与活力变化的关系。结果表明酶的活力丧失先于以紫外差吸收先谱、荧先发射谱和巯基暴露数目所监测到的构象变化。SDS滴定时引起的酶的荧光发射光谱的变化在低滴定度阶段随着SDS滴定量的增加,荧光强度下降,发射峰位红移,当SDS浓度达到2.1mmol/L时,荧光强度增大,继续增加SDS滴定量,荧光强度又降低,发射峰位红移直至终态。紫外差吸收光谱随着SDS溶液的加入,281nm.287nm和292nm的负差吸收峰增大。CD光谱结果表明在本实验所用的SDS浓度范围内,SDS对人肌肌酸激酶的二级结构几乎没有影响。上述结果支持了酶的活性部位构象柔性的观点。 相似文献
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重组白细胞介素2体外折叠的实验研究 总被引:2,自引:0,他引:2
包涵体中的重组蛋白贩提后可以在变性状态下纯化,而纯化后的体外折叠(即复性)是基因工程下处理中的重要环节。荧光光谱研究表明,IL-2分子折叠过程中荧光强度逐渐减小,最大发射峰由316nm红移到348nm。以Trp残基的暴露程序反映分子的折叠状态,GM-CSF在折叠过程中的荧光强度有类似变化,凝胶排阻HPLC可以检测折叠过程中的聚合体,而反相HPLC可以将IL-2分成三个相互独立的异构体色谱峰,据此可 相似文献
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核糖核酸(RNA)与稀土铽离子在pH5.0~6.5条件下能形成具有较强荧光的络合物,其激发峰在288nm处,发射峰为494nm及545nm处.RNA在0.1~10mg/L范围内与其荧光强度呈线性关系,其检出限为6.0×10 ̄(-8)mol/L.腺苷酸,尿苷酸,胞苷酸对RNA的干扰比较小,因此可在其存在下选择性地测定RNA. 相似文献
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低强度激光血管内照射对实验性糖尿病兔血液流变性质的影响 总被引:6,自引:1,他引:5
本实验用38只实验性糖尿病兔,分别采用1.2mw、1.5mw、2.2mw×50分钟三组不同剂量He—Ne激光,分两批作一次性血管内照射,测定其红细胞变形能力等8项血液流变学指标,并观察指标随时间的变化。结果表明:(1)激光照射后血液粘度降低,红细胞聚集性降低,且在24小时内就有反映,5—7天仍然有效;(2)能提高红细胞变形能力,以照射后第3天最明显。5—7天后会回复照射前水平。此结果可为低强度激光血管内照射临床应用选择照射频度提供参考(3)可改善红细胞刚度,但时间上反映较迟 相似文献
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The origination of the peak at 730 nm in the delayed fluorescence (DF) spectrum of chloroplasts was studied using various optical analysis methods. The DF spectrum showed that the main emission peak was at about 685 nm, with a small shoulder at 730 nm when the chloroplast concentration was < 7.8 microg/mL. The intensity of the peak at 685 nm decreased, while the intensity of the peak at 730 nm increased, when the chloroplast concentrations were increased from 7.8 to 31.2 microg/mL. With the concentration increasing, the peak at 730 nm became dominant while the peak at 685 nm finally disappeared. The DF decay kinetic curves showed that the intensity of the peak at 730 nm decayed as the same speed as the intensity of the peak at 685 nm during the entire relaxation process (0.5-30.5 s). With the excitation wavelength at 685 nm, the emission intensity was stronger in the excitation spectrum at 730 nm. The absorption spectrum demonstrated that the ratio A(685):A(730) remained almost constant when the chloroplast concentration increased. The results suggest that the peak at 730 nm appearing in DF is mainly contributed by the fluorescence of photosystem I (PSI), generated by the re-absorption of 685 nm band DF. 相似文献
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The fluorescence from the purple membrane protein (PM) of Halobacterium halobium and its relation to the primary photochemical events have been studied. The emission spectrum at 77 degrees K has structure, with peaks at 680, 710-715, and 730-735 nm. The excitation spectrum shows a single peak centered at 580 nm. This and a comparison of the fluorescence intensity at 77 degrees K under a variety of conditions with the amounts of the bathoproduct (or K, the only photoproduct seen at this temperature) formed suggest that the source of the fluorescence is the purple membrane itself, not the photoproduct. From the difference in several of their properties, we suggest that the fluorescing state of the pigment is different from the excited state which leads to photoconversion. 相似文献
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Priyangshu Rana Borthakur Simanta Hazarika Pradeep Kumar Gupta Anurup Gohain Barua 《Food biophysics》2013,8(4):297-301
A 405 nm diode laser is used to excite fluorescence of juices of raw and ripe lemons. Emission bands appear approximately at 520 nm and 670 nm. Fluorescence intensity ratio F520/F670 is determined for the two stages. Variation in the fluorescence intensity ratio is observed during the process of ripening or growth of the fruit. Time-resolved spectra at this excitation wavelength reveal two decay times at both the stages at the emission wavelength of 520 nm, and two decay times at the raw stage and one decay time at the ripe stage at 670 nm. 相似文献
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Analyses of chlorophylls a and b and P700 in the wheat leaves grown for 8 days under illumination with white light at different intensities suggested selective formation of photosystem 1 of the photosynthesis at low light intensities. This was confirmed for the two types of chloroplasts isolated from leaves grown at light intensities of 1.1 and 240 μ W/cm2, respectively, by measuring their pigment compositions, activities of photosystems 1 and 2, and absorption and fluorescence spectra. The chloroplasts developed at the low intensity showed properties only of photosystem 1 while those developed at the high intensity showed properties of both photosystems 1 and 2. Only photosystem 1 particles were obtained by fractionation of low intensity chloroplasts by treatment with digitonin followed by centrifugation, while high intensity chloroplasts could be fractionated into photosystem-1 and photosystem-2 particles. When the leaves grown at low light intensity were illuminated with strong light, photosystem 2 was developed. The fluorescence emission spectrum of low intensity chloroplasts at 77°K showed two peaks at 685 and 734 nm, and the spectrum of high intensity chloroplasts showed three peaks at 685, 697 and 740 nm. 相似文献
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We demonstrate the direct 1064 nm two-photon excitation of hematoporphyrin derivative (HPD), a complex mixture of photosensitizing porphyrins which is selectively retained in tumor tissue and used in cancer photochemotherapy. Although 1064 nm is outside of the one-photon HPD absorption spectrum, two-photon induced fluorescence from HPD was observed following excitation by the 20 ns output of an amplified, Q-switched Nd-YAG laser at peak power levels of 0.1 to 3 GW/cm2. Evidence for the successful two-photon excitation to vibrational levels of the S1 state consists of the observation of the known HPD fluorescence spectrum exhibiting peaks at approximately 615 and 675 nm, with the observed two-photon induced fluorescence intensity exhibiting a quadratic dependence on the excitation laser intensity as required for a direct two-photon process. More generally, these results suggest the possibility for the achievement of photosensitized oxidations utilizing photons of lower energy than that required for single photon excitation, offering the potential for both greater selectivity and a reduction in competing photochemical processes. 相似文献
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Picosecond time-resolved fluorescence spectroscopy of K-590 in the bacteriorhodopsin photocycle. 总被引:3,自引:2,他引:1
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The fluorescence spectrum of a distinct isometric and conformational intermediate formed on the 10(-11) s time scale during the bacteriorhodopsin (BR) photocycle is observed at room temperature using a two laser, pump-probe technique with picosecond time resolution. The BR photocycle is initiated by pulsed (8 ps) excitation at 565 nm, whereas the fluorescence is generated by 4-ps laser pulses at 590 nm. The unstructured fluorescence extends from 650 to 880 nm and appears in the same general spectral region as the fluorescence spectrum assigned to BR-570. The transient fluorescence spectrum can be distinguished from that assigned to BR-570 by a larger emission quantum yield (approximately twice that of BR-570) and by a maximum intensity near 731 nm (shifted 17 nm to higher energy from the maximum of the BR-570 fluorescence spectrum). The fluorescence spectrum of BR-570 only is measured with low energy, picosecond pulsed excitation at 590 nm and is in good agreement with recent data in the literature. The assignment of the transient fluorescence spectrum to the K-590 intermediate is based on its appearance at time delays longer than 40 ps. The K-590 fluorescence spectrum remains unchanged over the entire 40-100-ps interval. The relevance of these fluorescence data with respect to the molecular mechanism used to model the primary processes in the BR photocycle also is discussed. 相似文献
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发菜藻胆体的分离和光谱特性的研究 总被引:2,自引:0,他引:2
完整藻胆体和不完整藻胆体的吸收峰都在618nm。完整藻胆体的室温荧光峰位于670nm以上,而不完整藻胆体则在670nm以下。完整藻胆体的77K荧光发射光谱中只有648nm一个荧光发射带;而在不完整藻胆体,则有2个或3个发射带,它们位于684nm,666nm和648nm,依次属于别藻蓝蛋白-B,别藻蓝蛋白和C-藻蓝蛋白的荧光。 相似文献
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完整藻胆体和不完整藻胆体的吸收峰都在618nm。完整藻胆体的室温荧光峰位于670nm 以上,而不完整藻胆体则在670nm以下。完整藻胆体的77K荧光发射光谱中只有648nm一个荧光发射带;而在不完整藻胆体,则有2个或3个发射带,它们位于684nm,666nm和648nm, 依次属于别藻蓝蛋白 — B,别藻蓝蛋白和C — 藻蓝蛋白的荧光。 相似文献
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The kinetics of in vivo state transitions in mesophyll and guard cell chloroplasts monitored by 77 k fluorescence emission spectra 总被引:2,自引:1,他引:1
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Fluorescence emission spectral peaks at 685, 695 and 730 nanometers (F685, F695, and F730) were recorded 77 K from diluted leaf tissue and epidermal powders prepared from Saxifraga cernua. The time course for state 1 to state 2 transitions was monitored as changes in the ratios of the three emission peaks. During illumination with light 2 (580 nm) the F730/F695 and F730/F685 ratios increased within minutes to establish a condition characteristic of state 2. A major difference between the two chloroplast types was the more rapid establishment of state 2 by mesophyll chloroplasts. An increase in light 2 intensity caused an increase in the magnitude of the F730/F695 ratio for both chloroplast types and, for guard cell chloroplasts, a decrease in the time required to establish the new ratio. The role of reversible phosphorylation of the light-harvesting chlorophyll a/b protein complex in regulating state transitions for both mesophyll and guard cell chloroplasts was assessed using DCMU and sodium fluoride, a specific phosphatase inhibitor. DCMU-treated mesophyll and epidermal tissues failed to show a state 1-state 2 transition. NaF-treated tissues attained state 2 but lacked the ability to revert back to state 1. 相似文献