Cyst Distribution and Hatching Pattern of Chirocephalus ruffoi (Crustacea,Anostraca) in an Experimental Undisturbed Pool

The pattern of cyst distribution in the absence of turbation and their hatching behaviour were studied in an outdoor artificial pool, where just differentiated adults of the anostracan Chirocephalus ruffoi (sex ratio 1:3) lived until it dried up. The horizontal and vertical distributions of cysts in the pool bed were determined. The comparison between cyst bank estimate (Mura , 2004) and the actual number of cysts counted in the pool bed revealed an estimate error of 20.9%. Resting eggs occurred only in the upper 2.5 cm thick soil sections and decreased within this section as depth increased. Peripheral areas of the pool contained significantly larger numbers of cysts than the central area. Multiway analysis on the results recorded in hatching success (nested ANOVA) revealed that the differences were significantly affected by initial soil conditions, treatment and vertical distribution of cysts. Among these factors, vertical distribution (sections nested in cores) was the most influential. Hatching success was significantly inversely related to depth. Differences in the timing of hatching depending on the above considered factors were also noted. A nearly synchronous hatching pattern was observed only for cysts from initially dry sediment of the uppermost layers. In all successively deeper layers, hatching showed multiple peaks and was increasingly delayed and erratic (already mentioned). ANCOVA within each of the experimental conditions revealed significant differences in hatching frequencies (time as covariate) depending on sediment depth. Within any given layer, ANCOVA revealed a significant influence of initial sediment conditions and treatment on the timing of hatching. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Improving the Electrokinetic Remediation of Nickel-, Cadmium-, and Lead-contaminated Sediment

In this work, the simultaneous application of bipolar electrodes and a longer cathodic compartment length without agents addition was used as an enhanced electrokinetic treatment of Ni-, Cd-, and Pb-contaminated sediment. Conventional and enhanced treatments were investigated and their efficacy assessed by comparing the residual metals content in the sediment with values given by Dutch standards. After conventional treatment removals of 20%, 19%, and 31% for Ni, Cd, and Pb were achieved, whereas the enhanced treatment achieved removal efficacies of 36% for Ni, 42% for Cd, and 43% for Pb. Results indicate that the simultaneous application of the techniques mentioned is effective for removing Ni and Pb whereas Cd was not removed to the desired level. Here we show that the addition of chemicals is not necessary to increase the efficacies of electrokinetic sediment treatments, so the simultaneous application of the techniques mentioned can be described as environmentally friendly.     

The Importance of Vertical and Horizontal Dimensions of the Sediment Matrix in Structuring Nematodes Across Spatial Scales

Intensive surveys have been conducted to unravel spatial patterns of benthic infauna communities. Although it has been recognized that benthic organisms are spatially structured along the horizontal and vertical dimensions of the sediment, little is known on how these two dimensions interact with each other. In this study we investigated the interdependence between the vertical and horizontal dimensions in structuring marine nematodes assemblages. We tested whether the similarity in nematode species composition along the horizontal dimension was dependent on the vertical layer of the sediment. To test this hypothesis, three-cm interval sediment samples (15 cm depth) were taken independently from two bedforms in three estuaries. Results indicated that assemblages living in the top layers are more abundant, species rich and less variable, in terms of species presence/absence and relative abundances, than assemblages living in the deeper layers. Results showed that redox potential explained the greatest amount (12%) of variability in species composition, more than depth or particle size. The fauna inhabiting the more oxygenated layers were more homogeneous across the horizontal scales than those from the reduced layers. In contrast to previous studies, which suggested that reduced layers are characterized by a specific set of tolerant species, the present study showed that species assemblages in the deeper layers are more causal (characterized mainly by vagrant species). The proposed mechanism is that at the superficial oxygenated layers, species have higher chances of being resuspended and displaced over longer distances by passive transport, while at the deeper anoxic layers they are restricted to active dispersal from the above and nearby sediments. Such restriction in the dispersal potential together with the unfavorable environmental conditions leads to randomness in the presence of species resulting in the high variability between assemblages along the horizontal dimension.     

Strain, stress and energy in lipid bilayer induced by electrostatic/electrokinetic forces

Lipid bilayer was deformed by the electrostatic/electrokinetic forces induced by the fixed charges on the top monolayer-solution interface. The strains, stresses and energy were simulated using finite element method. The elastic moduli of the heads were four times greater than those of tails sections, but were individually isotropic. The physics of the situation was evaluated using a coupled system of linear elastic equations and electrostatic-electrokinetic (Poisson-Nernst-Planck) equations. The Coulomb force (due to fixed charges in the electric field), and the dielectric force (due to uneven electric field and the solution-membrane permittivity mismatch) bend the membrane, but unevenly. Whereas the bottom monolayer extends vertically (towards charged surface), the top monolayer compresses. In contrast the top monolayer extends horizontally, but the bottom monolayer compresses. The horizontal normal stress is higher in the heads than in the tails sections, but is similar in two monolayers, whereas the vertical normal stress is small. The horizontal normal stress is associated with horizontal normal strain, and vertical with both vertical and horizontal strain. Surprisingly, the shear stress (an indicator where the membrane will deform), is greater in the tails sections. Finally, the elastic energy (which is clearly greater in the heads sections) is dominated by its horizontal component and peaks in the middle of the membrane. The shear component dominates in the tails sections, and is minimal in the membrane center. Even spatially uniform external force thus leads to complex membrane deformation and generates complex profiles of stress and elastic energy.     

The impact of aquatic macrophyte (Salix sp. and Cladium mariscus (L.) Pohl.) removal on habitat conditions and macroinvertebrates of tufa barriers (Plitvice Lakes, Croatia)

The effects of aquatic macrophyte (willows and sawgrass) removal on flow velocity, tufa deposition, POM dynamics, and macroinvertebrate community structure were studied in the tufa barrier habitats of the barrage system of Plitvice Lakes, Croatia. Samples were collected from two hydraulic habitats (fast > 100 cm s⁻¹ and slow < 100 cm s⁻¹) at both a control (no macrophytes removed) and impact (macrophytes removed) site. Samples were collected with a core sampler (four layers in vertical profile of barrier bed) monthly on 6 dates before and 7 dates after the removal of macrophytes. Macrophytes were removed in May 2002 at the impact site. After the macrophyte removal flow velocity decreased significantly at both hydraulic habitats. Retarded flow resulted in: (a) a decrease in macroinvertebrate density and diversity since most of the taxa were rheophilic (preferring habitats with higher flow velocity) and (b) an increase in POM concentrations (FPOM and UPOM) since decreases in flow velocity facilitate particle deposition in lotic habitats. The effects of macrophyte removal were present, and diminish along the vertical sediment profile of the barrier bed. Tufa deposition was not influenced by the macrophyte removal. Electronic supplementary material Electronic supplementary material is available for this article at and accessible for authorised users.     

Increased removal of nickel and organics in the upflow anaerobic sludge bed filter reactor using sulfate and sulfide additions

Glucose (total organic carbon: 1,200 mg/l) and nickel (0 to 40 mg/l) were added to an anaerobic upflow sludge bed filter reactor. The removal efficiencies of total organic carbon and nickel were maintained to 95% and 98.5%, respectively, since nickel was precipitated with sulfide which was converted from sulfate added at 80 mg SO4-S/l by sulfate reducing bacteria. Sulfate therefore enhances its organic removal efficiency of AUBF reactor under the presence of heavy metal.     

Role of nickel in high rate methanol degradation in anaerobic granular sludge bioreactors

The effect of nickel deprivation from the influent of a mesophilic (30 degrees C) methanol fed upflow anaerobic sludge bed (UASB) reactor was investigated by coupling the reactor performance to the evolution of the Methanosarcina population of the bioreactor sludge. The reactor was operated at pH 7.0 and an organic loading rate (OLR) of 5-15 g COD l(-1) day(-1) for 191 days. A clear limitation of the specific methanogenic activity (SMA) on methanol due to the absence of nickel was observed after 129 days of bioreactor operation: the SMA of the sludge in medium with the complete trace metal solution except nickel amounted to 1.164 (+/-0.167) g CH(4)-COD g VSS(-1) day(-1) compared to 2.027 (+/-0.111) g CH(4)-COD g VSS(-1) day(-1) in a medium with the complete (including nickel) trace metal solution. The methanol removal efficiency during these 129 days was 99%, no volatile fatty acid (VFA) accumulation was observed and the size of the Methanosarcina population increased compared to the seed sludge. Continuation of the UASB reactor operation with the nickel limited sludge lead to incomplete methanol removal, and thus methanol accumulation in the reactor effluent from day 142 onwards. This methanol accumulation subsequently induced an increase of the acetogenic activity in the UASB reactor on day 160. On day 165, 77% of the methanol fed to the system was converted to acetate and the Methanosarcina population size had substantially decreased. Inclusion of 0.5 muM Ni (dosed as NiCl(2)) to the influent from day 165 onwards lead to the recovery of the methanol removal efficiency to 99% without VFA accumulation within 2 days of bioreactor operation.     

Modelling pollutant removal in a pilot-scale two-stage subsurface flow constructed wetlands

The hydraulic behaviour and effluent pollutant concentrations in a pilot-scale two-stage subsurface flow constructed wetland for treatment of municipal wastewater have been simulated. The experimental pilot plant is located in San Michele di Ganzaria (Eastern Sicily) and consists of four lines of two-stage subsurface flow constructed wetlands for secondary or tertiary treatment of municipal wastewater. The first stage, for each line, consists of a horizontal flow bed, while in the second stage a vertical flow bed operates for two lines and a horizontal flow bed for the other two. Phragmites sp. was used as vegetation in two lines while the other two lines are without plants. The HYDRUS-2D software was applied to describe flow and single-solute transport, while the multi-component reactive transport module CW2D was used to model the transformation and elimination processes of organic matter, nitrogen and phosphorus. Tracer studies and chemical wastewater analyses were carried out to calibrate and validate the transport model. In general, the simulation results obtained show a good match with the measured data for water flow, tracer experiments and pollutant removal processes.     

Succession of cable bacteria and electric currents in marine sediment

Filamentous Desulfobulbaceae have been reported to conduct electrons over centimetre-long distances, thereby coupling oxygen reduction at the surface of marine sediment to sulphide oxidation in sub-surface layers. To understand how these ‘cable bacteria'' establish and sustain electric conductivity, we followed a population for 53 days after exposing sulphidic sediment with initially no detectable filaments to oxygen. After 10 days, cable bacteria and electric currents were established throughout the top 15 mm of the sediment, and after 21 days the filament density peaked with a total length of 2 km cm⁻². Cells elongated and divided at all depths with doubling times over the first 10 days of <20 h. Active, oriented movement must have occurred to explain the separation of O₂ and H₂S by 15 mm. Filament diameters varied from 0.4–1.7 μm, with a general increase over time and depth, and yet they shared 16S rRNA sequence identity of >98%. Comparison of the increase in biovolume and electric current density suggested high cellular growth efficiency. While the vertical expansion of filaments continued over time and reached 30 mm, the electric current density and biomass declined after 13 and 21 days, respectively. This might reflect a breakdown of short filaments as their solid sulphide sources became depleted in the top layers of the anoxic zone. In conclusion, cable bacteria combine rapid and efficient growth with oriented movement to establish and exploit the spatially separated half-reactions of sulphide oxidation and oxygen consumption.     

Oxidation and Mobility of Trivalent Chromium in Manganese-Enriched Clays during Electrokinetic Remediation

This article presents the results of an investigation that assessed the extent and effect of oxidation of Cr(III) in manganese-enriched clays on the electrokinetic remedial efficiency. Because chromium commonly exists along with nickel and cadmium at contaminated sites, the effects of changes in chromium redox chemistry on the migration of the coexisting nickel and cadmium was also studied. Bench-scale electrokinetic experiments were conducted using two different clays: kaolin, a typical low buffering soil, and glacial till, a high buffering soil. Tests were performed with 1000?mg/kg of Cr(III), 500?mg/kg of Ni(II), and 250?mg/kg of Cd(II), both with and without the presence of 1000?mg/kg of manganese. All of these experiments were conducted under a constant voltage gradient of 1.0?VDC/ cm. The experimental results showed that in the presence of manganese, percentages of oxidation of Cr(III) into Cr(VI) ranged from 67% in kaolin to 28% in glacial till even before the application of induced electric potential. The low extent of oxidation of Cr(III) in glacial till may be attributed to the initial precipitation of Cr(III) as Cr(OH)₃ resulting from high soil pH, reducing aqueous Cr(III) concentrations present within the soil. In kaolin, Cr(III), Ni(II), and Cd(II) under electric potential migrated toward cathode and precipitated near the cathode due to high soil pH. When manganese was present in kaolin, Cr(VI) that was formed due to the oxidation of Cr(III) migrated toward anode and adsorbed to the soil surfaces near the anode region due to low soil pH. However, remaining Cr(III) as well as Ni(II), and Cd(II) migrated towards and precipitated near the cathode due to high soil pH. In kaolin, the migration of Ni(II) and Cd(II) was retarded in the presence of manganese due to a larger soil zone of elevated pH near the cathode. In glacial till, the migration of Cr(III), Ni(II) and Cd(II) was insignificant due to precipitation resulting from high soil pH caused by the high buffering capacity of the soil. Cr(VI) that resulted from the partial oxidation of Cr(III) in the presence of manganese, however, migrated toward the anode. Overall, this study demonstrated that the effects of manganese on Cr(III) oxidation in low buffering soils can be significant, which can in turn affect the extent and direction of chromium migration under induced electric potential.     

In-situ removal of ammonium and lactate through electrical means for hybridoma cultures

Ammonium and lactate are two known toxic products detrimental to mammalian cell growth and productivity. An electrokinetic technique, utilizing an electrophoretic mechanism, was developed to remove these cellular wastes in-situ from suspension hybridoma (ATCC CRL-1606) cultures to enhance cell growth and productivity. This technique applies continuously a dc electric field to selectively remove the electrically charged wastes. The experiments were shown to be successful in the removal of externally added 10 rnM ammonium and 45 mM lactate while maintaining the chemostatic condition of culture medium in a cell-free condition under an electric current density of 50 A/m(2). Toxic levels of ammonium were added, ranging from 7.5 to 12.5 mM, at the start of the hybridoma culture, and the applied dc electric fields were able to completely remove these added materials. This in turn released the inhibition and restored the cell growth. Finally, this electrokinetic technique was applied to the batch and glutamine fed-batch hybridoma cultures. At an applied electric current density of 50 A/m(2), this was able to completely remove cell-produced ammonium and increased the cell growth and antibody titer by 30% to 50%, respectively, compared to the control experiment in the absence of the electric field. Lastly, the applied electric current density of 50 A/m(2) did not affect cellular functionalities such as glucose and glutamine consumption and antibody productivity.(c) 1995 John Wiley & Sons, Inc.     

Electrokinetic transport of an NAPL-degrading microorganism through sandy soil bed

The use of electrokinetic injection and transport for the distribution of NAPL-degradingCandida lipolytica CL180 in a sandy soil bed was studied. After the injection of the cell into cathodic side of the bed, an electric current was applied. The strain CL180 exhibited unidirectional movement towards anode in the presence of an electric current, implying that CL180 has a negative surface charge. There existed a lag time during which there was no observed cell transport. After a lag time for transport initiation, cell population decreased in the section near to cathode and increased in the middle and anode sections. When the magnitude of applied electric current increased, the lag time for transport initiation and the time for maximum population at the center of the bed were both shortened. The average electrokinetic mobility was estimated as 0.0188-0.0225 cm²/h·V. Electrokinetic transport rate of CL180 increased proportionally to the applied electric current.     

Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake, Lake Biwa

Methane oxidation coupled to denitrification is mediated by 'Candidatus Methylomirabilis oxyfera', which belongs to the candidate phylum NC10. The distribution of putative denitrifying methane-oxidizing bacteria related to "M. oxyfera" was investigated in a freshwater lake, Lake Biwa, Japan. In the surface layer of the sediment from a profundal site, a phylotype closely related to "M. oxyfera" was most frequently detected among NC10 bacteria in PCR analysis of the 16S rRNA gene. In the sediment, sequences related to "M. oxyfera" were also detected in a pmoA gene library. The presence of NC10 bacteria was also confirmed by catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR indicated that the abundance of the "M. oxyfera"-related phylotype was higher in the upper layers of the profundal sediment. The horizontal distribution of the putative methanotrophs in lake sediment was also analyzed by DGGE, which revealed that their occurrence was restricted to deep water areas. These results agreed with those in a previous study of another freshwater lake, and suggested that the upper layer of the profundal sediments is the main habitat for denitrifying methanotrophs.     

Impact of wet deposited nickel on the cation content of a mat-forming lichen Cladina stellaris

The impact of experimentally sprayed aqueous nickel solution on the concentrations of potassium, calcium, magnesium and nickel in three horizontal strata (top, 0-20 mm; middle, 20-40 mm; and base, 40-60 mm) of the cushion-forming lichen Cladina stellaris was investigated. The experimental nickel deposition range used corresponded with that from the pristine forests of the Finnish border to polluted industrial sites of Russian Kola Peninsula (0-1000 mg Ni(2+) m(-2) year(-1)). The lichen mat retained ca. 31-66% of the nickel deposited during two growing seasons and the relative retention efficiency was highest at the low deposition end. The concentrations of cations in lichen thalli were significantly reduced only after the highest nickel deposition. Furthermore, the separate horizontal strata responded differently to nickel exposure indicating that the cation exchange sites of the top stratum were not completely saturated by nickel even after the most severe treatment. However, nickel deposited in high doses caused considerable reduction in potassium concentration indicating damage to cell membranes. Episodically deposited high concentrations of nickel can probably affect membrane integrity before detectable changes in total concentrations of cations in the lichen thallus take place. Thus, ratios of total concentrations of cations in the lichen thallus are fairly insensitive to nickel deposition, which reduces the risk of compounding effects when the ratios are used to indicate long-term acid deposition in areas with multiple pollution problems such as Kola Peninsula.     

Convolutional Virtual Electric Field for Image Segmentation Using Active Contours

Gradient vector flow (GVF) is an effective external force for active contours; however, it suffers from heavy computation load. The virtual electric field (VEF) model, which can be implemented in real time using fast Fourier transform (FFT), has been proposed later as a remedy for the GVF model. In this work, we present an extension of the VEF model, which is referred to as CONvolutional Virtual Electric Field, CONVEF for short. This proposed CONVEF model takes the VEF model as a convolution operation and employs a modified distance in the convolution kernel. The CONVEF model is also closely related to the vector field convolution (VFC) model. Compared with the GVF, VEF and VFC models, the CONVEF model possesses not only some desirable properties of these models, such as enlarged capture range, u-shape concavity convergence, subject contour convergence and initialization insensitivity, but also some other interesting properties such as G-shape concavity convergence, neighboring objects separation, and noise suppression and simultaneously weak edge preserving. Meanwhile, the CONVEF model can also be implemented in real-time by using FFT. Experimental results illustrate these advantages of the CONVEF model on both synthetic and natural images.     

低覆盖度下两种行带式固沙林内风速流场和防风效果

研究在风速为10m/s和15m/s的风洞实验条件下,覆盖度为20%和25%的单行一带和两行一带模式乔木固沙林内的水平和垂直空间的风速变化情况,达到对两种不同模式的风速流场和防风效果进行比较分析的目的。通过分析得出:两种配置模式都形成了风影区和风速加速区相互组合的复杂的水平流场结构。两种模式对垂直空间风速影响相近,根据对不同高度风速的不同影响划分为微变化层(20-35 cm)、显著变化层(6-12 cm)和稳定变化层(0.4-3 cm)层次,两种模式在这几个层次风速表现出相同的变化规律。两行一带模式在第一带前降低水平空间风速的效果低于单行一带模式,第一带后高于后者。降低垂直空间风速规律为:对0.4-50 cm高度的风速均有一定的降低作用,0.4-12 cm高度的风速的降低效果较显著,且两行一带模式降低近地表(0.4 cm)风速的效果要高于单行一带模式。     

Isolation and characterization of heavy polycyclic aromatic hydrocarbon-degrading bacteria adapted to electrokinetic conditions

Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria capable of growing under electrokinetic conditions were isolated using an adjusted acclimation and enrichment procedure based on soil contaminated with heavy PAHs in the presence of an electric field. Their ability to degrade heavy PAHs under an electric field was individually investigated in artificially contaminated soils. The results showed that strains PB4 (Pseudomonas fluorescens) and FB6 (Kocuria sp.) were the most efficient heavy PAH degraders under electrokinetic conditions. They were re-inoculated into a polluted soil from an industrial site with a PAH concentration of 184.95 mg kg^?1. Compared to the experiments without an electric field, the degradation capability of Pseudomonas fluorescens and Kocuria sp. was enhanced in the industrially polluted soil under electrokinetic conditions. The degradation extents of total PAHs were increased by 15.4 and 14.0 % in the electrokinetic PB4 and FB6 experiments (PB4 + EK and FB6 + EK) relative to the PB4 and FB6 experiments without electrokinetic conditions (PB4 and FB6), respectively. These results indicated that P. fluorescens and Kocuria sp. could efficiently degrade heavy PAHs under electrokinetic conditions and have the potential to be used for the electro-bioremediation of PAH-contaminated soil, especially if the soil is contaminated with heavy PAHs.     

Cascade Electric Field Enhancement in the Orthogonal-Nanorod Structures

The transverse mode electron oscillations contribute to most of the surface-enhanced Raman scattering (SERS) intensity from the nanorod array substrates. To enhance the transverse mode electron oscillation and improve the SERS enhancements, the local electric field distribution of the orthogonal-nanorod structures, composed of two parallel horizontal nanorods in between two parallel vertical nanorods, has been studied. The local electric fields of the longitudinal mode along the horizontal nanorods act as the excitation for the transverse mode electron oscillations in vertical nanorods, leading to the cascade enhancements of the electric fields around the vertical nanorods. In addition, the plasmon peaks of the longitudinal modes can be tuned by changing the lengths and the widths of the horizontal nanorods and the separations between horizontal and vertical nanorods. These results would be much helpful to engineer SERS substrates to obtain larger SERS enhancements.     

Miocene invertebrate trace fossils from a braided river environment,western Nebraska,U.S.A

Lower Miocene cross-stratified sands of the Gering and Monroe Creek Formations exposed on Scotts Bluff National Monument in western Nebraska, U.S.A., were deposited by migrating sand bars in a braided river system similar to the modern Platte River in eastern Nebraska and, like the Platte, contain local lenses of parallel stratified sediment that accumulated in ponded areas of abandoned channels. During times of low discharge, broad areas of river bar sands and abandoned channel sediments were subaerially exposed on the Miocene river plain. These sediments, like those exposed in the Platte River today, were subjected to burrowing by insects and other animals.Trace fossils in Lower Miocene braided river deposits are: vertical shelter burrows, horizontal deposit-feeding burrows, bioturbated layers, and vertical passageways between bioturbated layers. The burrows are cylindrical to sub-cylindrical in cross-section, internally meniscate or massive, generally non-branching, and smooth walled. Shelter burrows are similar in shape and size to recent burrows dug by beetles in river sediment for protection from day-time temperatures, to pass the night, and to hibernate. The shelter burrows, deposit-feeding burrows, and vertical passageways in the Lower Miocene sediments occur in four distinct “populations” with modal diameters of 1–2, 3–4, 7–8, and 10–12 mm. The occurrence of both vertical and horizontal burrows in all four “populations” suggests that they could have been made by the same insect. “Populations” with modal diameters of 1–2, 3–4, and 7–8 mm also occur in modern Platte River sediment and are made by tiger-beetle larvae (3–4 mm) and heterocerid (1–2 mm) beetles. Miocene shelter burrows, deposit-feeding burrows, bioturbated layers, and vertical passageways, therefore, could have been formed by different types of beetles, and/or larval instars and adults of the same beetle species.     

Direct measurements of carbon and carbonate export from a coral reef ecosystem (Moorea Island , French Polynesia)

The export of carbon and carbonate from coral reefs was investigated through a multidisciplinary investigation of the hydrological, geochemical, sedimentological and biological features of Tiahura reef on the northwestern coast of Moorea Island (French Polynesia). The hydrology of the fore-reef is characterised by prevailing longshore western currents and a strong thermocline. As revealed by turbidity structures (benthic and intermediate nepheloid layers) and by the amount of particles collected by near-bottom sediment traps, horizontal and downslope advections of particles dominate over offshore vertical transport. The exported material is rich in carbonate (ca. 80%) and poor in organic matter (ca. 4%). Sedimentation rates at 430 m depth, i.e. definitive export, reached 209.6 mg m^-2 d^-1 (dry weight). Estimates of carbon and carbonates export for Tiahura reef also reported here represent respectively 47% and 21% of the organic and inorganic carbon produced within the reef.