Chemical constituents from the leaves of Cinnamomum parthenoxylon (Jack) Meisn. (Lauraceae)

Phytochemical investigation of the ethanolic extract from the leaves of Cinnamomum parthenoxylon (Jack) Meisn. led to the isolation of (3R, 4R, 3′R, 4′R)-6,6′-dimethoxy-3, 4, 3′, 4′-tetrahydro-2H, 2′H-[3, 3′]bichromenyl-4, 4′-diol (1), 4-hydroxybenzaldehyde (2), 1,2,4-trihydroxybenzene (3), kaempferol-3-O-α-l-rhamnoside (4), herbacetin (5), quercetin-3-O-α-l-rhamnoside (6), daucosterol (7), and β-sitosterol (8). The structures were established by extensive analysis of their MS and NMR spectroscopic data and comparison with literature data. In the present research, all of the isolated compounds 1–8 are reported for the first time in the species C. parthenoxylon. Compounds 1–6 were firstly isolated from genus Cinnamomum. Compounds 1, 3, 5 and 6 have not been reported from any species in Lauraceae family. The chemotaxonomic significance of the isolated compounds is discussed.     

关于樟科润楠属和鳄梨属的分类界线问题

讨论了润楠属Machilus Nees与鳄梨属Persea Mill.的分类界线, 认为两属的主要区别如下: 在润楠属中, 花被裂片相等或近相等, 极少外轮明显短于内轮, 在果期为纸质, 极少为薄革质, 伸长, 强度反曲, 极少张开与不反曲, 几乎宿存, 极少脱落, 如果脱落时则内、外轮从基部完全脱落; 花柱早落; 分布亚洲热带及亚热带地区。而在鳄梨属中, 花被裂片极不相等, 外轮明显短于内轮, 很少近相等, 在果期为革质到木质, 很少为薄革质, 张开或直立, 几乎不反曲, 绝大部分宿存或内轮在距基部的1/3到1/2处脱落; 花柱有时宿存; 分布美洲。两属的界线清楚, 不宜合并。把产于越南北部的Persea balansae Airy Shaw和产于苏门答腊的P. sumatrana Kosterm.转移到Machilus中, 从而提出Machilus balansae (Airy Shaw) F. N. Wei &; S. C. Tang和M. sumatrana (Kosterm.) F. N. Wei &; S. C. Tang两个新组合。     

Chemical constituents of the bark of Endlicheria oreocola (Lauraceae) from Colombia

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Alkene lactones from Persea fulva (Lauraceae): Evaluation of their effects on tumor cell growth in vitro and molecular docking studies

The new alkene lactone, (3E)-5,6-dihydro-5-(hydroxymethyl)-3-docdecylidenefuran-3(4H)-one (1), named majoranolide B, and three alkene lactones known as majorenolide (2), majoranolide (3) and majorynolide (4) were obtained from the aerial parts of Persea fulva (Lauraceae). The structures were elucidated in light of extensive spectroscopic analysis, including 1D, 2D NMR (¹H, ¹³C, ¹H-¹H-COSY, HMBC and HSQC) and HR-ESI-MS. These compounds were screened for their in vitro antiproliferative activity in rat C6 glioma and astrocyte cells using MTT assay and in silico by molecular docking against targets that play a central role in controlling glioma cell cycle progression. Majoranolide (3) is the most active compound with IC₅₀ 6.69 µM against C6 glioma cells, followed by the compounds 1 (IC₅₀ 9.06 µM), 2 (IC₅₀ 12.04 µM) and 4 (IC₅₀ 41.90 µM). The alkene lactones 1–3 exhibited lower toxicity in non-tumor cells when compared to glioma cells. Molecular docking results showed that majoranolide establishes hydrogen bonds with all targets through its α,β-unsaturated-γ-lactone moiety, whereas the long-chain alkyl group binds by means of several hydrophobic bonds. In the present study, it can be concluded from the anti-proliferative activity of isolates against C6 glioma cells that lactone constituents from P. fulva could have a great potential for the control of C6 glioma cells.     

樟科润楠属植物ITS 序列贝叶斯分析及其系统学意义

应用nrDNA Internal Transcribed Spacer ( ITS) 序列, 使用Bayesian 分析法对樟科润楠属的系统学问题进行了初步探讨, 结果表明润楠属在鳄梨属群中本身作为一独立分支, 是一个自然的单系类群; 由于其花被裂片果期宿存且强烈反转与同产自亚洲的楠属和油丹属存在清晰界线。本研究否定了前人依据花被片外面被毛情况及果实大小所建立的润楠属属下系统, 但同时暗示着花序类型可能是解决润楠属下系统演化的关键性状。本研究对解决润楠属属内种间的系统演化关系尚有不足, 建议今后补充更多的属内物种以及寻找新的分子标记进行更深入的研究。     

Ecological and genetic differentiation in Persea boninensis (Lauraceae) endemic to the Bonin (Ogasawara) Islands

Persea boninensis (Lauraceae) is an endemic tree species distributed throughout the Bonin Islands. It grows in a wide range of environments from dry to mesic forests, and has multiple flowering peaks that may correspond to different habitats on Chichijima Island of the Bonin Islands. We predicted that P. boninensis is differentiated into two groups with different habitats on these islands. We examined and compared the flowering phenology, morphology, and genetics of populations of species growing in dry and mesic forests. We also performed preliminary artificial crossing experiments. Based on our results, P. boninensis on the Chichijima Islands can be clearly divided into two genetic groups with different habitats and flowering times. Although the flowering time difference could act as an effective pre‐zygotic isolation mechanism between the two groups, there was still a 1‐month overlap in flowering time. Furthermore, our artificial crossing experiments between the two groups resulted in plants that set seeds. Therefore, there was no evidence of reproductive isolation after fertilization. Differences in flowering time as well as in growth habitat will have to occur to maintain genetic differentiation between the two groups of P. boninensis.     

Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model

Aniba parviflora (Meisn .) Mez (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a tremendous commercial value in the perfumery industry; it is popularly used as flavoring sachets and aromatic baths. In Brazilian folk medicine, A. parviflora is used to treat victims of snakebites. Herein, we analyzed the chemical composition of A. parviflora bark essential oil (EO) and its effect on the growth of human hepatocellular carcinoma HepG2 cells in vitro and in vivo. EO was obtained by hydrodistillation and characterized by GC-MS and GC-FID. The main constituents of EO were linalool (16.3±3.15), α-humulene (14.5±2.41 %), δ-cadinene (10.2±1.09 %), α-copaene (9.51±1.12 %) and germacrene B (7.58±2.15 %). Initially, EO's cytotoxic effect was evaluated against five cancer cell lines (HepG2, MCF-7, HCT116, HL-60 and B16-F10) and one non-cancerous one (MRC-5), using the Alamar blue method after 72 h of treatment. The calculated IC₅₀ values were 9.05, 22.04, >50, 15.36, 17.57, and 30.46 μg/mL, respectively. The best selectivity was for HepG2 cells with a selective index of 3.4. DNA Fragmentation and cell cycle distribution were quantified in HepG2 cells by flow cytometry after a treatment period of 24 and 48 h. The effect of EO on tumor development in vivo was evaluated in a xenograft model using C.B-17 SCID mice engrafted with HepG2 cells. In vivo tumor growth inhibition of HepG2 xenograft at the doses of 40 and 80 mg/kg were 12.1 and 62.4 %, respectively.     

中国樟科木姜子属植物纪要

描述一新种即沧源木姜子Litsea cangyuanensis J．Li et H．W．Li;一中国新纪录种白叶木姜子L．albescens（Hook．f．）D．G．Long;3个新异名：大叶木姜子L．chunii Cheng var．longipedicellata Yang f．latifolia Yang归并到高山木姜子L．chunii Cheng,狭叶桂北木姜子M．subcoriacea Yang et P．H．Huang val．stenophylla Yang et P．H．Huang归并入桂北木姜子L．subcoriacea Yang et P．H．Huang,狭叶华南木姜子L．greenmaniana Allen val．angustifolia Yang et P．H．Huang归入华南木姜子L．greenmaniana Allen;思茅木姜子L．szemaois（H．Liou）J．Li et H．W．Li作为新等级提升为种;并对秃净木姜子L．kingii Hook．f．与山鸡椒L．cubeba（Lour．）Pers．两者间的差异进行澄清,对台湾产的黄肉树应采用L．hypophaea Hayata和广西产的蜂窝木姜子应采用L．foveola Kosterm．进行了讨论。一个新名称：L．sinoglobosa J．Li et H．W．Li替代同名的L．globosa Yang et P．H．Huang。     

Phenolic derivatives with free-radical-scavenging activities from Ixeridium gracile (DC.) Shih

Phytochemical investigation of the Tibetan medicinal plant Ixeridium gracile resulted in the isolation and identification of twelve flavonoids and two coumarins, compounds 1-14, the dimeric '2alpha,3alpha-epoxy-5,7,3',4'-tetrahydroxyflavan-(4beta-->8)-epicatechin' (1) being a new constituent. The free-radical-scavenging potentials of different extract fractions as well as of the pure compounds towards the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical were evaluated, and are discussed in terms of structure-activity relationship (SAR). The flavonoids were found to be the major constituents contributing to the free-radical-scavenging activity of I. gracile, but the high concentration of coumarins additionally contributed to the observed activity.     

中国樟科木姜子属植物纪要*

描述一新种即沧源木姜子Litsea cangyuanensis J. Li et H. W. Li; 一中国新纪录种白叶木姜子L1albescens
( Hook. f. ) D. G. Long; 3 个新异名: 大叶木姜子L1 chunii Cheng var1longipedicellata Yang f1 latif olia Yang 归并到高
山木姜子L1 chunii Cheng, 狭叶桂北木姜子M1 subcoriacea Yang et P. H. Huang var. stenophylla Yang et P. H.
Huang 归并入桂北木姜子L1 subcoriacea Yang et P. H. Huang, 狭叶华南木姜子L1greenmaniana Allen var1 angustifolia
Yang et P. H. Huang 归入华南木姜子L1greenmaniana Allen; 思茅木姜子L1szemaois ( H. Liou) J. Li et H. W. Li
作为新等级提升为种; 并对秃净木姜子L1 kingii Hook. f. 与山鸡椒L1 cubeba ( Lour . ) Pers. 两者间的差异进
行澄清, 对台湾产的黄肉树应采用L1 hypophaea Hayata 和广西产的蜂窝木姜子应采用L1f oveola Kosterm. 进
行了讨论。一个新名称: L1 sinoglobosa J. Li et H. W. Li 替代同名的L1 globosa Yang et P. H. Huang 。     

Three new species of Ocotea (Lauraceae) from southern Mexico

Three new species ofOcotea (O. disjuncta, O. iridescens, andO. rovirosae) from southern Mexico are described and illustrated.Ocotea disjuncta is related to theO. effusa group, whileO. iridescens seems to be related to theO. heydeana group. The relationships ofO. rovirosae are not clear.      

中国樟科润楠属植物一些种类修订

对中国樟科润楠属植物一些种进行了分类修订。随着研究工作的深入 ,发现过去发表的一些新种不能成立 ,应予合并 ,即嘉道理楠 =信宜润楠 ;长序润楠 =浙江润楠 ;密脉润楠 =刨花润楠 ;芳槁润楠 =黄心树 ;荔波润楠 =粉叶润楠。     

Intraspecific variability of flavonoid glycosides and styrylpyrones from leaves of Cryptocarya mandioccana Meisner (Lauraceae)

Chemical variability in populations of Cryptocarya mandioccana Meisner was investigated using chromatographic profiles of flavonoid glycosides and styrylpyrones from leaves of 57 trees from three sites in the Atlantic rain forest of São Paulo State – Brazil. The flavonoid profiles of the leaves from all trees were similar. However, four chemotypes were recognized by qualitative and quantitative differences in styrylpyrones. Chemotype F showed lower contents of these polyketides than chemotypes FS1, FS2 and FS3, which were characterized by styrylpyrones formed from different numbers of acetate units. Chemotypes with higher levels of styrylpyrones were more frequent in southern regions, with soils of higher K⁺, Ca²⁺ and Mg²⁺ content. Soil and climate could indicate a genetic or environmental influence on styrylpyrones in leaves of C. mandioccana.