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1.
目的:研究孕酮(PROG)对新生大鼠低氧缺血后脑内基质金属蛋白酶3(MMP-3)表达的影响。方法:建立新生大鼠低氧缺血性脑损伤动物模型,伊文思兰(EB)染色和电镜观察新生鼠低氧缺血性脑损伤血一脑屏障的通透性改变;免疫印迹(Western blot)方法检测大脑皮层MMP-3表达。结果:电镜显示低氧缺血组血-脑屏障完整性明显破坏:EB染色结果表明低氧缺血组血-脑屏障通透性明显高于假手术组,差异极显著(P〈0.01),孕酮组血-脑屏障通透性明显低于低氧缺血组,有显著性差异(P〈0.05);Western blot结果显示低氧缺血组MMP-3蛋白表达显著高于假手术组(P〈0.01);孕酮组MMP-3蛋白表达显著低于低氧缺血组(P〈0.05)。结论:孕酮通过减少MMP-3的表达,降低血一脑屏障的损伤,这可能是其发挥脑保护作用的机制之一。 相似文献
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当归对宫内缺氧新生大鼠大脑皮质神经元与VEGF mRNA表达的影响 总被引:1,自引:0,他引:1
目的 探讨宫内缺氧对新生大鼠大脑皮质神经元与VEGF mRNA表达的影响以及当归的调控作用.方法 孕14 d健康SD雌性大鼠15只,随机分为对照组、缺氧组和当归组各5只,于孕14 d开始将当归组与缺氧组孕鼠置于低张氧浓度三气培养箱中,制作胎鼠宫内缺氧模型,此前一小时按8 mL/kg分别给予当归和生理盐水尾静脉注射,对照组不缺氧,余同缺氧组.三组孕鼠分娩当日每窝随机选取新生鼠4只,取脑组织多聚甲醛固定,石蜡包埋切片、NSE mRNA、VEGF mRNA原位杂交,400倍拍照、IPP6.0软件图像分析.结果 缺氧组新生大鼠大脑皮质NSE mRNA阳性细胞数较对照组减少,积分光密度值(IOD)降低(P<0.05),VEGF mRNA阳性细胞IOD值升高(P<0.05);当归组新生大鼠大脑皮质NSE mRNA阳性细胞数较缺氧组增多、IOD值增高(P<0.05),VEGF mRNA阳性细胞IOD值增高(P<0.05).结论 宫内缺氧可致新生大鼠大脑皮质神经元受损,当归注射液对此损伤有一定保护作用,其机制可能是通过上调大脑皮质VEGF mRNA的表达而使缺氧环境改善. 相似文献
3.
陶存山 《国外医学:分子生物学分册》2001,23(2):115-117
分子生物学技术推动了缺血性脑损伤的实验研究进展,大量研究从分子水平揭示了脑组织损伤(死亡)发生的机制并创造了新的治疗方法。术文就缺血性脑损伤研究中的分子生物学应用、立及早基因的表达、细胞凋亡机制及基因治疗方法等进行综述。 相似文献
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IGF-1对缺血性脑损伤大鼠脑内神经发生的影响 总被引:3,自引:0,他引:3
目的:建立大鼠单侧局灶脑缺血模型,观察胰岛素样生长因子-1(IGF-1)对局灶脑缺血后的鼠脑神经发生及增殖后细胞生存的影响.方法:用健康雄性SD大鼠建立大脑中动脉阻塞(MCAO)模型,随机分成假手术组,缺血对照组和IGF-1治疗组.各组再按不同的治疗时间分为7d、14d、28d、42d组.免疫组化法观察BrdU、PSA-NCAM的变化,免疫双标法观察BrdU/PSA-NCAM、BrdU/MAP2和BrdU/GFAP的共同表达变化.结果:BrdU标记细胞和PSA-NCAM标记细胞计数均在缺血后第7d最多,分别是缺血对照组的4.0倍和1.8倍,是假手术组的9.9倍和5.4倍.BrdU和PSA-NCAM双标细胞在缺血发生后双侧SVZ和DG区可以检测到,于第7d计数最多,之后逐渐降低;而BrdU和MAP2以及BrdU和GFAP双标细胞却从第14d开始逐渐增多,直到第42d.随着BrdU/PSA-NCAM双标阳性表达的逐渐降低,BrdU/MAP2双标阳性表达逐渐增高,呈现此消彼涨的变化.结论:IGF-1侧脑室注射后,在早期(7d内)诱导了缺血性脑损伤后神经细胞的增殖;在中期(7d-14d)诱导了新生细胞的迁移;在后期(14d后)伴随着迁移的进行新生细胞逐渐发生了分化. 相似文献
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成年鼠缺血性脑损伤诱导nestin的表达 总被引:16,自引:1,他引:15
应用免疫组化和免疫荧光双标技术结合激光共聚焦扫描显微镜,观察缺血性脑损伤后脑内nestin的表达及其细胞类型。实验观察结果为,再灌后1天,在缺血中心区可见nestin阳性突起;再灌后3天和1周时,除缺血中心区外,周边I、Ⅱ、Ⅲ区均有nestin阳性突起主要与GFAP共存;2周时,nestin阳性突起变粗、变长,并与NSE的共存明显增多。上述研究结果提示,脑缺血可诱导大鼠脑缺血区域表达nestin,该表达可能与神经细胞的修复有关。 相似文献
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目的探讨神经干细胞(NSCs)移植对大鼠创伤性脑损伤(TBI)整合素(integrin)表达的影响。方法从E14大鼠胚胎分离NSCs,进行原代培养及传代培养;对NSCs进行诱导分化;采用免疫细胞化学技术对NSCs和其分化为神经元的表型进行鉴定。采用改良的Feeney法制备创伤性脑损伤模型。利用脑立体定位仪和微量注射泵进行NSCs脑内移植。采用免疫组织化学技术、免疫印迹技术和RT—PCR技术检测在移植后不同时间脑组织损伤区整合素的表达。结果在培养基中,NSCs呈球团状悬浮生长,Nestin表达阳性。用含10%胎牛血清的培养基对NSCs进行体外诱导分化后第2d,多数细胞伸出突起,以后突起逐渐延长,分支增加。分化后第5d,部分细胞呈βⅢ-微管蛋白阳性。整合素阳性产物主要表达于细胞膜,呈棕黄色。在对照组及移植组均可见阳性细胞表达。在不同时间点,NSCs移植组移植点及其周围脑组织中整合素的mRNA表达均显著高于对照组(P〈O.01)。整合素的蛋白表达结果和tuRNA表达结果相一致。结论移植NSCs至TBI大鼠损伤脑组织,在移植点周围脑组织中整合素的表达显著增加。 相似文献
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大鼠液压冲击脑损伤脑干c-fos mRNA表达的定位观察 总被引:2,自引:0,他引:2
目的:研究大鼠中度侧位液压冲击脑损伤时脑干c-fos mRNA及其表达产物Fos变化规律。方法:雄性SD大鼠,随机分为正常对照组、手术对照组和损伤组。损伤组动物均给以0.2MPa液压冲击脑损伤,按冲击后处死时间不同再分为5min、15min、30min、1h、2h、4h、8h和12h组。应用免疫组织化学和原位杂交方法观察c-fos在脑干的表达。结果:脑冲击后15min-12h,Fos阳性细胞数逐渐 相似文献
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摘要 目的:探讨香草醛对新生大鼠缺氧缺血性脑损伤(HIBI)的神经保护作用及机制。方法:参考Rice-Vannucci方法建立HIBI大鼠模型。HIBI大鼠建模后立即腹腔注射20 mg/kg(HIBI+20Van组)或40 mg/kg(HIBI+40Van组)的香草醛,每隔12 h给药,连续7 d。然后评估大鼠的神经行为及脑组织中IL-1β、IL-6和TNF-α的水平。对BV2小胶质细胞进行氧糖剥夺/复氧(OGD/R)处理,并用20 μM香草醛培养。通过Western blot及免疫荧光检测HMGB1、NF-κB p65、SIRT1、MyD88和TLR4的表达水平。通过乳酸脱氢酶(LDH)释放测定试剂盒测定用不同BV2细胞培养基处理的原代神经元的LDH释放。结果:与HIBI组比较,HIBI+20Van组和HIBI+50Van组新生大鼠的前肢悬吊时间和旷场得分均升高,脑组织中的IL-1β、IL-6和TNF-α的水平均降低。香草醛均升高了HIBI大鼠和OGD/R处理的BV2细胞质中的SIRT1的表达水平,降低了TLR4、MyD88和HMGB1的表达水平及细胞核中NF-κB p65的表达水平(P<0.05)。香草醛降低了原代神经元的LDH释放量(P<0.05)。结论:香草醛通过调节SIRT1/HMGB1/TLR4/MyD88/NF-κB信号通路抑制HIBI引起的神经炎症,从而提高HIBI大鼠的神经功能。 相似文献
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目的:通过对动物模型的制作模拟新生儿围产期缺氧缺血性脑损伤,研究其脑组织病理变化,为新生儿缺氧缺血性脑损伤的病理生理的研究以及进一步有效的治疗提供实验基础。方法:将40只7d新生昆明小鼠分四组,分别为正常组(A组)、单侧颈总动脉结扎组(B组)、单侧颈总动脉结扎+缺氧组(C组)和双侧颈总动脉结扎组(D组)。单侧颈总动脉结扎组(B组)行右侧颈总动脉结扎;单侧颈总动脉结扎+缺氧组(C组)行右侧颈总动脉结扎后将其置于20℃的恒温50mL密闭容器中,分不同的时间将其取出;双侧颈总动脉结扎组(D组)行双侧颈总动脉结扎,各组术后均送回母鼠身边继续母乳喂养,三天后再作病理检测。结果:行单侧颈总动脉结扎加缺氧60min时,小鼠结扎侧皮质及海马区出现病理改变,随着缺氧时间延长(90rain、100min、120min)病变范围逐渐扩大,病理改变越明显。结论:本实验显示单侧颈总动脉结扎同时缺氧一定时间可以导致小鼠脑组织损伤,脑细胞发生病理改变,且皮层及海马区域的神经细胞对缺氧缺血最为敏感,从而为进一步研究新生儿缺氧缺血性脑损伤提供了较为可靠的模型。 相似文献
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Early effects of hypoxia/reoxygenation on VEGF,Ang-1, Ang-2 and their receptors in the rat myocardium: Implications for myocardial angiogenesis 总被引:6,自引:0,他引:6
Fiorani M De Sanctis R Scarlatti F Vallorani L De Bellis R Serafini G Bianchi M Stocchi V 《Molecular and cellular biochemistry》2000,213(1-2):145-153
The oxidized form of vitamin C (dehydroascorbic acid, DHA) completely and irreversibly inactivates recombinant human hexokinase type I, in a pseudo-first order fashion. The inactivation reaction occurs without saturation, indicating that DHA does not form a reversible complex with hexokinase. Further characterization of this response revealed that the inactivation does not require oxygen and that dithiothreitol, while able to prevent the DHA-mediated loss of enzyme activity, failed to restore the activity of the DHA-inhibited enzyme. Inactivation was not associated with cleavage of the peptide chain or cross-linking. The decay in enzymatic activity was however both dependent on deprotonation of a residue with an alkaline pKa and associated with covalent binding of DHA to the protein. In addition, inactivation of hexokinase decreased or increased, respectively, in the presence of the substrates glucose or MgATP. Finally, amino acid analysis of the DHA-modified hexokinase revealed a decrease of cysteine residues.Taken together, the above results are consistent with the possibility that covalent binding of the reagent with a thiol group of cysteine is a critical event for the DHA-mediated loss of hexokinase activity. 相似文献
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Chronic hypoxia attenuates resting and exercise-induced VEGF, flt-1, and flk-1 mRNA levels in skeletal muscle. 总被引:3,自引:0,他引:3
I M Olfert E C Breen O Mathieu-Costello P D Wagner 《Journal of applied physiology》2001,90(4):1532-1538
Vascular endothelial growth factor (VEGF) is a hypoxia-inducible angiogenic mitogen. However, chronic hypoxia is generally not found to increase mammalian skeletal muscle capillarity. We sought to determine the effect of chronic hypoxia (8 wk, inspired O2 fraction = 0.12) on skeletal muscle gene expression of VEGF, its receptors (flt-1 and flk-1), basic fibroblast growth factor, and transforming growth factor-beta1. Wistar rats were exposed to chronic hypoxia (n = 12) or room air (n = 12). After the exposure period, six animals from each group were subjected to a single 1-h treadmill exercise bout (18 m/min on a 10 degrees incline) in room air while the remaining six animals served as rest controls. Morphological analysis revealed that chronic hypoxia did not increase skeletal muscle capillarity. Northern blot analyses showed that chronic hypoxia decreased resting VEGF, flt-1, and flk-1 mRNA by 23, 68, and 42%, respectively (P < 0.05). The VEGF mRNA response to exercise was also decreased (4.1- and 2.7-fold increase in room air and chronic hypoxia, respectively, P < 0.05). In contrast, neither transforming growth factor-beta1 nor basic fibroblast growth factor mRNA was significantly altered by chronic hypoxia. In conclusion, prolonged exposure to hypoxia attenuated gene expression of VEGF and its receptors flt-1 and flk-1 in rat gastrocnemius muscle. These findings may provide an explanation for the lack of mammalian skeletal muscle angiogenesis that is observed after chronic hypoxia. 相似文献
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《Biotechnic & histochemistry》2013,88(7):486-494
Porcine embryo mortalities cause economic losses. Development of the placental vascular bed is required for successful gestation and postnatal survival. We studied the temporal and spatial distributions of vascular endothelial growth factor (VEGF), placenta growth factor (PlGF) and their receptors, Flt-1 and Flk-1. We used crossbred swine placental tissues from 30, 60, 80, 90 and 114 (term) days of gestation. Both VEGF and PlGF were present during gestation. At early pregnancy and at term, VEGF probably acts through Flt-1; during intermediate periods, its function is mediated by Flk-1. By day 90, factors other than members of VEGF family appear to be involved. 相似文献
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应用RTPCR技术,从人脐静脉内皮细胞中扩增出编码人可溶性血管内皮细胞生长因子
(VEGF)受体Flt1胞外区前四个结构域的基因片段,亚克隆至pUCl8质粒进行测序,将目的基因片段连接至链霉菌表达载体pSGLgpp,获得重组质粒pSGLgppF,将其转化至Streptomyces lividans TK24, 获得基因工程菌株Sreptomyces lividans (pSGLgppF),对其培养上清液进行SDSPAGE及Western blot分析,结果
显示,在636kD处有特异性条带出现,表明sFLT1在链霉菌中获得了成功表达,受体配基结合实验显示表达产物与VEGF可特异性结合,表明其具有配基结合生物活性。 相似文献
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目的研究血管内皮生长因子(VEGF)及其受体Flt-1在实验性左侧精索静脉曲张(ELV)大鼠附睾组织中的表达变化,探讨它们与精索静脉曲张(VC)的关系及致男性不育的病理生理学机制。方法建立青春期雄性SD大鼠ELV模型,采用免疫组化SP法检测ELV及对照组附睾中VEGF和Flt-1的表达。结果 VEGF和Flt-1蛋白在大鼠附睾中均有表达,并具有细胞和区域特异性。ELV4周时双侧附睾中VEGF蛋白的表达明显上调(P<0.01),8周时则显著下降(P<0.01);而Flt-1蛋白在ELV4周左侧显著下降(P<0.01),右侧未见明显差异(P>0.05),8周组均显著下降(P<0.01)。结论 ELV引起大鼠附睾中VEGF和Flt-1表达量发生变化,可能影响精子的成熟,是VC引起男性生育力下降甚至不育的原因之一。 相似文献
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目的:探讨褪黑素(MT)对大鼠急性肺损伤(ALI)时肺脏的保护作用及其可能机制。方法:将大鼠随机分为4组:对照组;脂多糖(LPS)组;地塞米松(DEX)和MT处理组。各组动物分别于气道内滴注后3、6和12h检测肺组织髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;免疫组化检测细胞间黏附分子-1(ICAM-1)在肺组织的表达。结果:LPS组SOD活性较对照组明显降低(P<0.01),而MPO活性与MDA含量以及ICAM-1的表达则显著升高(P<0.01);应用MT及DEX均显著缓解上述变化(P<0.05或P<0.01)。结论:MT对ALI时的肺脏起明显的保护作用,其机制可能与MT清除自由基及抑制ICAM-1的表达有关。 相似文献
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本研究旨在克隆和表达人血管内皮生长因子受体-1胞外Ⅲ区蛋白,并测定其生物学活性。应用RT-PCR法从人脐静脉内皮细胞中克隆Flt-1胞外Ⅲ区基因片段,经测序鉴定后再克隆到原核表达载体pAYZ中,构建出的表达载体pAYZflt-1Ⅲ转化大肠杆菌16C9后,用低磷培养基诱导表达目的蛋白;采用E-tag亲和层析柱纯化目的蛋白;用SDS-PAGE、Western blotting和BCA法对其进行定性、定量检测鉴定;用ELISA、损伤愈合试验和Transwell法检测靶蛋白生物学活性。克隆的Flt-1胞外Ⅲ区基因经测序鉴定正确。所构建的pAflt-1Ⅲ表达载体经低磷培养基诱导后高表达出可溶性Flt-1胞外Ⅲ区蛋白,产量约为1.1mg/L;ELISA结果显示该蛋白可以结合VEGF165,并表现为剂量依赖性,其与配体结合的解离常数Kd为1.180pmol/L。损伤愈合试验和Transwell结果显示该蛋白可以抑制VEGF165(50ng/ml)和bFGF(100ng/mL)诱导的脐静脉内皮细胞的迁移,并呈剂量依赖性。这将为今后开展人flt-1基因Ⅲ区的功能研究及其单抗研制奠定了实验基础。 相似文献
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Tripterine is a chemical isolated from a traditional Chinese herb which had been testified for its anti-inflammatory and immunosuppressive activities in a previous study. However, little is known about the effects and mechanism of action of Tripterine on treating lupus nephritis. In the present study we investigated the effect of Tripterine on the F1 hybrids of New Zealand Black (NZB) and New Zealand White (NZW) mice which functioned as a model of human systemic lupus erythematosus (BW F1 mice) and evaluated the possible mechanism implicated in the mRNA expression of TGF-beta1 and collagen IV expression of the BW F1 mice kidney tissue. Different doses of Tripterine were injected peritoneally to BW F1 mice at different stages to study the preventive effects of Tripterine on lupus nephritis glomerulosclerosis and its mechanisms. Twenty-four hour urine protein excretion, serum anti-dsDNA antibodies and the expression of collagen type IV were examined by immunohistochemistry while the expression of TGF-beta1 mRNA was detected by RT nested PCR. Tripterine decreased urine protein excretion and the level of serum anti-dsDNA antibodies and also suppressed the expression of collagen type IV and TGF-beta1 mRNA in the murine kidney tissue. Administration of Tripterine before the occurrence of proteinuria had much greater protective effects than if it was administered after the occurrence of proteinuria. No significant difference was found between the 3 mg/kg/week Tripterine-treated-group and the 6 mg/kg/week Tripterine-treated-group. Tripterine had a definite protective effect on glomerulosclerosis of the lupus murine model. Tripterine could significantly reduce the amount of urine protein excretion, suppress the formation of serum anti-dsDNA antibodies, it could also efficiently decrease the expression of renal collagen type IV probably due to its suppressive effect on the expressions of local TGF-(1 mRNA) in this model. 相似文献
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Xu T Wang X Cao M Wu X Yan Y Fu H Zhao W Gong P Ke K Gu X 《Journal of molecular histology》2012,43(3):335-342
BAG-1 protein was initially identified as a Bcl-2-binding protein. It was reported to enhance Bcl-2 protection from cell death, suggesting that BAG-1 represents a new type of anti-cell death gene. Moreover, recent study has shown that BAG-1 can enhance the proliferation of neuronal precursor cells, attenuate the growth inhibition induced by siah1. However, its function and expression in the central nervous system lesion are not been understood very well. In this study, we performed a traumatic brain injury (TBI) model in adult rats and investigated the dynamic changes of BAG-1 expression in the brain cortex. Double immunofluorescence staining revealed that BAG-1 was co-expressed with NEURON and glial fibrillary acidic protein (GFAP). In addition, we detected that proliferating cell nuclear antigen had the co-localization with GFAP, and BAG-1. All our findings suggested that BAG-1 might involve in the pathophysiology of brain after TBI. 相似文献