Aims: To investigate the genetic diversity and population structure of Macqueen’s Bustard in Iran, using two mitochondrial DNA loci.
Methods: Molecular diversity of the mitochondrial cytochrome oxidase c subunit I (COI) gene and part of the mitochondrial control region D-loop (in total 1183 base pairs) were analysed from 26 individual Macqueen’s Bustards from three regions of Iran.
Results: There was little variation in nucleotides and haplotypes in the populations for genes of both CR and COI. The population had free breeding and gene flow between the three study regions in Iran: Petregan, Ferdows and Yazd.
Conclusion: The use of molecular and genetic studies is essential to strengthen the protection of genetic diversity of the Macqueen’s Bustard. 相似文献
Aim: To identify the causes of limited gene flow in Begonia.
Methods: We examined the genetic structure among the populations of Begonia maxwelliana at the macro- and micro-spatial scales using microsatellites, measured seed dispersal range and observed flowering phenology.
Results: Population differentiation and structuring were detected at both the macro- and micro-scales. Dispersal range was short, and all populations showed similar reproductive behaviour.
Conclusions: The strong population differentiation and structuring among the populations studied imply that they are evolutionarily significant units and possible candidates for speciation. Geographical barriers and limited seed dispersal restrict gene flow in the populations, and these factors may be responsible for the rapid speciation and large diversity in the family. 相似文献
Area covered: This review summarizes the mechanisms of action and applications of genome editing tools, from RNA interference to clustered regularly interspaced short palindromic repeats-based systems, in research and in clinics. We describe their essential role in high-throughput genetic screens and, in particular, in functional proteomics studies, to identify diagnostic markers and therapeutic targets. Indeed, gene silencing and proteomics have been extensively integrated to study global proteome changes, posttranslational modifications, and protein–protein interactions.
Expert commentary: Functional proteomics approaches that leverage gene silencing tools have been successfully applied to examine the role of several genes in various contexts, leading to a deeper knowledge of biological pathways and disease mechanisms. Recent developments of gene silencing tools have improved their performance, also in terms of off-targets effects reduction, paving the way for a wider therapeutic application of these systems. 相似文献
Aims: Creating a baseline data set of epiphyte diversity in human settlements and assessing differences with assemblages of less disturbed habitats.
Methods: We surveyed the vascular epiphyte assemblages in 25 settlements of south-west Panama along a rainfall gradient. We tested how epiphyte assemblages were affected by human disturbance and climate by comparing our data to that of pastures and forest.
Results: Almost half (238 of 499) of all studied trees hosted at least one epiphyte. Altogether, 10,700 epiphytes of 56 species were found. Rainfall strongly affected both composition and diversity of epiphyte assemblages, whereas geographic proximity did not. In settlements, the species pool was considerably smaller than in pastures and undisturbed forest; however, settlements’ diversity was only significantly lower compared to pastures.
Conclusions: Epiphyte assemblages in settlements had a diminished species pool but diversity per tree was comparable to less disturbed habitats. Unsurprisingly, rainfall seems to be the main determinant of epiphyte diversity in rural settlements. 相似文献
Areas covered: This review discusses recent trends in proteomics and introduces new and developing approaches that can be applied to the study of protein-protein interactions (PPIs) underlying bacterial pathogenesis. The approaches examined encompass options for mapping proteomes as well as stable and transient interactions in vivo and in vitro. We also explored the coverage of bacterial and human-bacterial PPIs, knowledge gaps in this area, and how they can be filled.
Expert commentary: Identifying potential antimicrobial candidates is confounded by the complex molecular biology of bacterial pathogenesis and the lack of knowledge about PPIs underlying this process. Proteomics approaches can offer new perspectives for mechanistic insights and identify essential targets for guiding the discovery of next generation antimicrobials. 相似文献
Areas covered: We overview the recent achievements and discoveries in human bronchial proteomics by outlining how some of the different proteomic techniques/strategies are developed and applied in LC, TB, COPD, and CF. Also, the future roles of bronchial proteomics in predictive proteomics and precision medicine are discussed.
Expert commentary: Much progress has been made in bronchial proteomics. Owing to the advances in proteomics, we now have better ability to isolate proteins from desired cellular compartments, greater protein separation methods, more powerful protein detection technologies, and more sophisticated bioinformatic techniques. These all contributed to our further understanding of lung diseases and for biomarker and therapeutic target discoveries. 相似文献
Aims: To determine differences in diversity patterns between serpentine and non-serpentine communities.
Methods: A two-year study was conducted in native eastern Mediterranean grasslands. For each year 40 0.25 m2 plots were sampled across four pairs of sites, each of which contained a serpentine and an adjacent non-serpentine plant community. Alpha and beta diversity (variation in species composition among plots within localities), species composition and biomass production were determined. Total soil elemental concentrations and pH were also measured.
Results: Serpentine habitats were shown to support a lower alpha diversity relative to non-serpentine habitatas on a per plot basis. Differences in alpha diversity between the two substrates were associated with variation in soil chemistry rather than above-ground biomass production. Serpentine habitats also exhibited lower beta diversity, which was unrelated to variation in biomass production. The two contrasting communities presented distinct species composition.
Conclusions: Differences in diversity patterns between serpentine and non-serpentine communities in the eastern Mediterranean are influenced by soil chemistry rather than biomass production. 相似文献
Molecular data provide a more dynamic picture of polyploid evolution than has been traditionally espoused. Numerous studies have demonstrated multiple origins of both allopolyploids and autopolyploids. In several polyploid species studied in detail, multiple origins were found to be frequent on a local geographic scale, as well as during a short span of time. Molecular data strongly suggest that recurrent formation of polyploid species is the rule, rather than the exception. In addition, molecular data indicate that recurrent formation of polyploids has important genetic consequences, introducing considerable genetic variation from diploid progenitors into polyploid derivatives.
Molecular data also suggest a much more important role for natural autopolyploids than has been historically envisioned. In contrast to the longstanding view of autopolyploidy as being rare, molecular data continue to reveal steadily increasing numbers of well-documented autoploids having tetrasomic or higher-level polysomic inheritance. Although autopolyploidy undoubtedly occurs much less frequently than allopolyploidy in natural populations, it nonetheless has been a significant evolutionary mechanism. Molecular data also provide compelling genetic evidence that contradicts the traditional view of autopolyploidy as being maladaptive. Electrophoretic studies have revealed three important attributes of autopolyploids compared to their diploid progenitors: (1) enzyme multiplicity, (2) increased heterozygosity, and (3) increased allelic diversity. Genetic variability is, in fact, typically substantially higher in autopoloids than in their diploid progenitors. These genetic attributes of autopolyploids are due to polysomic inheritance and provide strong genetic arguments for the potential success of autopolyploids in nature.
In addition to providing numerous important insights into the formation of polyploids and the immediate genetic consequences of polyploidy, molecular data also have been used to study the subsequent evolution of polyploid genomes. Common hypotheses on the subsequent evolution of polyploid genomes include (1) gene silencing, eventually leading to extensively diploidized polyploid genomes; (2) gene diversification, resulting in regulatory or functional divergence of duplicate genes; and (3) genome diversification, resulting in chromosomal repatterning. Compelling, but limited, genetic evidence for all of these factors has been obtained in molecular analyses of polyploid species. The occurrence of these processes in polyploid genomes indicates that polyploid genomes are plastic and susceptible to evolutionary change.
In summary, molecular data continue to demonstrate that polyploidization and the subsequent evolution of polyploid genomes are very dynamic processes. 相似文献
Aims: We evaluated differences in species richness, species diversity and species dominance patterns in 16 plant communities as well as the degree to which differences were driven by climatic conditions in sandy dunes in Yucatán. We evaluated the importance of invasive species in mediating patterns of species diversity and species dominance patterns.
Results: We found wide variation in plant species richness, species diversity and species dominance patterns among communities that stems from broad climatic differences along dune systems. Invasive plants represent almost one-third of total species richness and seem to be drastically changing the species dominance patterns in these communities.
Conclusions: Regional climatic differences along the Yucatán north coast seems to be a major driver of plant diversity and species composition. Our findings suggest that invasive plants have successfully colonised and spread along the coast over the past 30 years. Even though invasive species do not alter spatial patterns of species diversity, they are becoming more dominant with potential detrimental consequences for native plants. 相似文献
Areas covered: HCV is associated with lipoprotein components such as apolipoproteins. These interactions play a key role in the viral life cycle, viral persistence and pathogenesis of liver disease. This review introduces first the role of apolipoproteins in lipoprotein metabolism, then highlights the molecular mechanisms of HCV-lipoprotein interactions and finally discusses their clinical impact.
Expert commentary: While the study of virus-host interactions has resulted in a improvement of the understanding of the viral life cycle and the development of highly efficient therapies, major challenges remain: access to therapy is limited and an urgently needed HCV vaccine remains still elusive. Furthermore, the pathogenesis of disease biology is still only partially understood. The investigation of HCV-lipoproteins interactions offers new perspectives for novel therapeutic approaches, contribute to HCV vaccine design and understand virus-induced liver disease and cancer. 相似文献
Areas covered: Summarized are the key immunology findings obtained with MS-based proteomics in the past five years, with a focus on inflammasome activation, global protein secretion, mucosal immunology, immunopeptidome and T cells. Special focus is on extracellular vesicle-mediated protein secretion and its role in immune responses.
Expert commentary: Proteomics is an essential part of modern omics-scale immunology research. To date, MS-based proteomics has been used in immunology to study protein expression levels, their subcellular localization, secretion, post-translational modifications, and interactions in immune cells upon activation by different stimuli. These studies have made major contributions to understanding the molecular mechanisms involved in innate and adaptive immune responses. New developments in proteomics offer constantly novel possibilities for exploring the immune system. Examples of these techniques include mass cytometry and different MS-based imaging approaches which can be widely used in immunology. 相似文献
Areas covered: The contribution of proteomics to the characterization of physiological modifications occurring during human development is presented. The proteomic studies carried out to elucidate the molecular mechanisms underlying different pediatric pathologies and to discover new markers for early diagnosis and prognosis of disease, comprising genetic and systemic pathologies, sepsis and pediatric oncology are thereafter reported. The investigations concerning milk composition in human and farm mammals are also presented. Finally, the chances offered by the integration of different -omic platforms are discussed.
Expert commentary: The growing utilization of holistic technologies such as proteomics, metabolomics and microbiomics will allow, in the near future, to define at the molecular level the complexity of human development and related diseases, with great benefit for future generations. 相似文献
Areas covered: This article reviews the current state of targeted and untargeted DIA mass spectrometry-based proteomic workflows, including SRM, parallel-reaction monitoring (PRM) and untargeted DIA (e.g., SWATH). Corresponding bioinformatics strategies, as well as application in biological and clinical studies are presented.
Expert commentary: Nascent application of highly-multiplexed untargeted DIA, such as SWATH, for accurate protein quantification from clinically relevant and disease-related samples shows great potential to comprehensively investigate biomarker candidates and understand disease. 相似文献
Aims: We evaluated seed weight variation within and between Trichloris crinita and Trichloris pluriflora across their geographical ranges in Argentina.
Methods: Genetic variation in seed weight was evaluated through a common garden experiment. To examine the possible role of such variation in local adaptation, we compared the seed weight of plants of populations raised in the common garden with seed weight variation and ecogeographical variables across their original habitats. We also evaluated experimentally the effects of seed weight variation upon osmotic stress tolerance at germination.
Results: Variation in seed weight existed in both species. Such variation had a genetic basis in T. crinita related to several ecogeographical variables. Larger seeds of T. crinita were associated with more stressful environments and produced larger seedlings under both osmotic stress and non-stress conditions.
Conclusions: Our results suggest that seed weight variation in T. crinita is likely adaptive, with large seed having an advantage during early developmental stages, particularly under stressful conditions. Such knowledge should prove helpful in selecting the most suitable populations for restoration and plant breeding. 相似文献
The acquisition of nutrients is discussed in terms of their physical availability in the culture medium and uptake from the medium. The effect on uptake of factors such as pH and water potential and the relationship with growth rates and medium depletion are examined in detail.
Finally, some effects of nutrients on morphogenesis of plants are reviewed. 相似文献