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1.
该研究以淀粉含量不同的两个木薯品种(辐选01和华南124)为材料,通过测定各品种不同生育期叶、茎和根的蔗糖含量及块根淀粉含量,分析了蔗糖合成、转运和块根淀粉积累过程的相关性.结果表明:与华南124相比,在整个生育期内辐选01叶、茎的蔗糖含量均较高,块根蔗糖含量在块根膨大初期以前高于华南124,块根膨大初期以后则相反.在木薯的整个生育期,与辐选01相比,华南124的淀粉合成量和淀粉合成速率均较低.叶和茎蔗糖含量的变化规律与淀粉合成速率的变化规律相反,即块根淀粉积累明显加快时叶和茎的蔗糖含量略呈下降趋势,而块根淀粉合成减慢时叶、茎的蔗糖含量又开始上升.随着生育期的延后,块根蔗糖含量越来越低.在块根形成初期,蔗糖含量最高的组织部位为块根,其次为茎秆,最低的是叶片;而在块根成熟期时则相反,即蔗糖含量最高的部位是叶片,其次为茎杆,块根的蔗糖含量最小.相关性分析结果表明,木薯叶片蔗糖含量与块根淀粉含量呈显著的正相关;茎秆蔗糖含量与块根淀粉积累量呈不显著的正相关;块根蔗糖含量与淀粉积累量呈显著的负相关.由此可见,木薯叶、茎和根蔗糖与块根淀粉积累过程密切相关,其中叶片合成蔗糖的能力与块根利用蔗糖的能力在淀粉的积累过程中发挥关键作用.该研究结果为木薯的生产选育与高效栽培提供了理论依据. 相似文献
2.
The contribution of plastidial phosphoglucomutase to the control of starch synthesis within the potato tuber 总被引:1,自引:0,他引:1
The aim of this work was to evaluate the extent to which plastidial phosphoglucomutase (PGM) activity controls starch synthesis within potato (Solanum tuberosum L. cv. Desirée) tubers. The reduction in the activity of plastidial PGM led to both a correlative reduction in starch accumulation and an increased sucrose accumulation. The control coefficient of plastidial PGM on the accumulation of starch was estimated to approximate 0.24. The fluxes of carbohydrate metabolism were measured by investigating the metabolism of [U-14C]glucose in tuber discs from wild-type and transgenic plants. In tuber discs the control coefficient of plastidial PGM over starch synthesis was estimated as 0.36, indicating that this enzyme exerts considerable control over starch synthesis within the potato tuber. 相似文献
3.
何首乌块根中异常结构的形成过程 总被引:4,自引:1,他引:3
何首乌的块根是一种常用的中药,块根内具有异常的次生结构。在块根的横切面上,自外至内依次为周皮、薄壁组织、排列成一圈大小不等的异常周韧维管束和中央维管柱。在块根形成以前,根的初生和次生结构都是正常的。以后,通常由围绕在初生韧皮纤维束周围的中柱鞘和次生韧皮薄壁组织细胞形成异常形成层,产生异常维管束。此外,还发现少数由中央维管柱分支而成。在块根膨大过程中,束内外以及维管柱次生木质部的薄壁组织细胞也分裂并增大。从而使块根中薄壁组织占80%左右。上述变化过程在不定根的中部开始,向上、下两 相似文献
4.
Starch Phosphorylation in Potato Tubers Proceeds Concurrently with de Novo Biosynthesis of Starch 总被引:5,自引:4,他引:1
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The in vivo phosphorylation of starch was studied in Solanum tuberosum cv Dianella and Posmo. Small starch granules contain 25% more ester-bound phosphate per glucose residue than large starch granules. The degree of phosphorylation was found to be almost constant during tuber development. Isolated tuber discs synthesize starch from externally supplied glucose at a significant rate. Tuber discs supplied with glucose and [32P]orthophosphate incorporate radiolabeled phosphorus into the starch. The level of 32P incorporation is proportional to the amount of starch synthesized. The incorporation of 32P from orthophosphate is correlated to de novo synthesis of starch, since the incorporation of 32P is diminished upon inhibition of starch synthesis by fluoride. Based on the amount of [14C]glucose phosphate isolated after hydrolysis of purified starch from tuber discs incubated in the presence of [U-14C]glucose, approximately 0.5% of the glucose residues of the de novo-synthesized starch are phosphorylated. This value is in general agreement with the observed levels of phosphorus in starch accumulated during tuber development. Thus, the enzyme system responsible for starch phosphorylation is fully active in the isolated tuber discs, and the starch phosphorylation proceeds as an integrated part of de novo starch synthesis. 相似文献
5.
Zhang L Häusler RE Greiten C Hajirezaei MR Haferkamp I Neuhaus HE Flügge UI Ludewig F 《Plant biotechnology journal》2008,6(5):453-464
Transgenic potato (Solanum tuberosum) plants simultaneously over-expressing a pea (Pisum sativum) glucose-6-phosphate/phosphate translocator (GPT) and an Arabidopsis thaliana adenylate translocator (NTT1) in tubers were generated. Double transformants exhibited an enhanced tuber yield of up to 19%, concomitant with an additional increased starch content of up to 28%, compared with control plants. The total starch content produced in tubers per plant was calculated to be increased by up to 44% in double transformants relative to the wild-type. Single over-expression of either gene had no effect on tuber starch content or tuber yield, suggesting that starch formation within amyloplasts is co-limited by the import of energy and the supply of carbon skeletons. As total adenosine diphosphate-glucose pyrophosphorylase and starch synthase activities remained unchanged in double transformants relative to the wild-type, they cannot account for the increased starch content found in tubers of double transformants. Rather, an optimized supply of amyloplasts with adenosine triphosphate and glucose-6-phosphate seems to favour increased starch synthesis, resulting in plants with increased starch content and yield of tubers. 相似文献
6.
The objective of this study was to determine the relationship between tuber weight and enzymatic activities involved in tuber starch synthesis. As tuber weight increased, the activities of sucrose synthetase, UDPG pyrophosphorylase, and granular starch synthetase escalated, whereas the activities of soluble starch synthetase and ADPG pyrophosphorylase stayed constant and that of phosphorylase declined. This suggests that when samples are taken to determine specific enzymatic activities, the sampling procedure should ensure that results do not vary because of differences in the tuber weight or size distribution. 相似文献
7.
嘉兰(Gloriosa superba L.)系百合科草本植物,其块茎含有秋水仙碱。本文研究块茎形成规律及其形成过程中物质变化。研究结果表明:块茎播后,新生的块茎在生长初期和后期生长速度都较慢,中期生长速度最快,也是嘉兰块茎产量形成的主要时期。块茎中营养物质主要是淀粉。新生块茎中淀粉含量,是随着新块茎的生长,其含量逐渐增高。叶片的光合速率和叶面积,从播后逐渐增加,开花前达到高峰,花后又逐渐降低。开花前如能增加光合叶面积,可为后期块茎生长提供较多物质。块茎中秋水仙碱含量是随着块茎的成熟与淀粉含量的渐增而增加,至收获期达到高峰。这一结果表明:收获未充分成熟的块茎,会降低秋水仙碱含量。 相似文献
8.
Anja G. J. Kuipers Wim J. J. Soppe Evert Jacobsen Richard G. F. Visser 《Plant molecular biology》1994,26(6):1759-1773
Transgenic plants of a tetraploid potato cultivar were obtained in which the amylose content of tuber starch was reduced via antisense RNA-mediated inhibition of the expression of the gene encoding granule-bound starch synthase (GBSS). GBSS is one of the key enzymes in the biosynthesis of starch and catalyses the formation of amylose. The antisense GBSS genes, based on the full-length GBSS cDNA driven by the 35S CaMV promoter or the potato GBSS promoter, were introduced into the potato genome by Agrobacterium tumefaciens-mediated transformation. Expression of each of these genes resulted in the complete inhibition of GBSS gene expression, and thus in the production of amylose-free tuber starch, in mature field-grown plants originating from rooted in vitro plantlets of 4 out of 66 transgenic clones. Clones in which the GBSS gene expression was incompletely inhibited showed an increase of the extent of inhibition during tuber growth. This is likely to be due to the increase of starch granule size during tuber growth and the specific distribution pattern of starch components in granules of clones with reduced GBSS activity. Expression of the antisense GBSS gene from the GBSS promoter resulted in a higher stability of inhibition in tubers of field-grown plants as compared to expression from the 35S CaMV promoter. Field analysis of the transgenic clones indicated that inhibition of GBSS gene expression could be achieved without significantly affecting the starch and sugar content of transgenic tubers, the expression level of other genes involved in starch and tuber metabolism and agronomic characteristics such as yield and dry matter content. 相似文献
9.
Jonathan I Watkinson Lori Hendricks Allan A Sioson Lenwood S Heath Hans J Bohnert Ruth Grene 《Plant Physiology and Biochemistry》2008,46(1):34-45
A drought screen identified accessions of Solanum tuberosum ssp. andigena that showed varying degrees of physiological acclimation or adaptation to repeated drought stress. The accessions also showed variable tuber phenotypes from small tubers that failed to develop in an accession that showed photosynthetic adaptation to normal tubers in an accession with a phenotype showing some degree of photosynthetic adaptation and acclimation. Using microarray data, we correlated the expression of genes associated with carbon metabolism with the tuber development phenotypes under drought. Genes associated with sucrose and starch metabolism showed responses consistent with starch deficiency in the adapted accession and normal starch deposition in the intermediate accession. Starch phosphorylase and glycogen bound starch synthase were induced in the adapted accession, which had abnormal tuber development. Genes associated with trehalose were induced in the intermediate accession with normal tuber development. Genes associated with respiration were also induced in the intermediate accession, and a pattern compatible with the existence of a 3PGA recovery pathway was revealed. Expression of thioredoxin genes also correlated with tuber development phenotypes under drought stress. The data suggest differential regulation of starch deposition in accessions of Andigena with different abilities to respond to drought stress. 相似文献
10.
Kinetin-induced tuberization of isolated stolons was investigated with regard to accumulation of labelled kinetin, starch, protein and nucleic acid synthesis. Using kinetin-8-14C, it was found that more labelled meterial appeared at the locus of tyber formation that in other parts of the stolon. Substantial accumulation was evident before visible signs of tuber formation. The basal portion of the stolon also accumulated substantial amounts of labelled material. Kinetin-treated stolons showed extensive starch accumulation which was not evident in gibberellic acid-treated or untreated stolons. Starch accumulation occurred before any visible sign of tuber formation. There was no marked differences in the ability of the apical 0.5 cm of kinetin-treated and untrated stolons to incorporate 14 C uridine into RNA and 14C leucine into TCA precipitable protein. From these results it was concluded that kinetin-induced tuber formation may not involve the synthesis of new proteins. It is suggested that kinetin may be regulating tuber formation by suppressing starch hydrolase activity and stimulating starch synthetase activity whereas gibberellic acid inhibits tuber formation by promoting starch hydrolase activity or by suppressing starch synthesizing enzymes. 相似文献
11.
Tauberger E; Hoffmann-Benning S; Fleischer-Notter H; Willmitzer L; Fisahn J 《Journal of experimental botany》1999,50(333):477-486
Transgenic potato tubers that overexpressed either a cytosolic or an
apoplastic invertase in the wild type or AGPase antisense background were
used to analyse the effect of invertase activity on cell expansion, starch
granule formation and turgor pressure during tuber development. Although
the transgenic plants did not develop a visible phenotype in aerial regions
the size and number of tubers were significantly modified in the various
lines. Transmission electron and light microscopy were performed to monitor
starch grain size and number, cell size and cell wall thickness. Water
potential, osmotic pressure, and indirectly, turgor pressure were
determined during the final stages of tuber development. Glucose levels
were high in transgenic tubers that overexpressed a yeast-derived
invertase. The number of starch grains per cell was almost identical in all
transgenic lines. However, the amount of starch was modified in the
transgenics as compared to the wild type. As expected, the size of starch
grains was reduced in all lines that expressed an AGPase antisense mRNA.
These results indicate that invertase activity and glucose levels do not
affect initiation of starch grain formation during the early stages of
tuber development, but growth of starch corns in the later stages of tuber
maturation. 相似文献
12.
Pyrophosphorylases in Solanum tuberosum: I. Changes in ADP-Glucose and UDP-Glucose Pyrophosphorylase Activities Associated with Starch Biosynthesis during Tuberization, Maturation, and Storage of Potatoes
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Sowokinos JR 《Plant physiology》1976,57(1):63-68
Changes in ADP-glucose and UDP-glucose pyrophosphorylase activities were followed during tuber development of Solanum tuberosum and prolonged storage at 4 and 11 C. Potato tuberization was accompanied by a sharp increase in starch synthesis simultaneous with a marked rise in ADP-glucose pyrophosphorylase activity. When tubers reached an average diameter of 1 centimeter (0.5 gram average tuber weight) and had already established 58% starch on a dry weight basis, ADP-glucose pyrophosphorylase increased 16- to 24-fold over its activity seen in low starch containing stolon tissue. During this same period UDP-glucose pyrophosphorylase increased approximately 2- to 3-fold. Although participation of UDP-glucose in starch formation can not be neglected, it is suggested that the onset of rapid non-photosynthetic potato tuber starch biosynthesis may be closely related to the simultaneous increase in ADP-glucose pyrophosphorylase activity. 相似文献
13.
The activity of enzymes involved in the conversion of sucrose to starch together with the distribution of 14C-labelled photosynthate and 4C-sucrose was studied in potato tubers showing a range of growth rates and growth patterns. Within a particular tuber the uptake of 14C from labelled photosynthate and the conversion to ethanol-insoluble 14C was greatest in the apical tissue where both the rate of production of new storage cells and starch synthesis were likely to be greatest. Uptake and conversion of 14C was lowest in the older tissue of the tuber base. Pre-treatment of tubers with gibberellic acid reduced the total input of 14C from labelled photosynthate, reversed the gradient in 14C uptake between apical and basal tuber tissue, increased the amount of 14C per g fresh weight in the basal tissue and decreased the conversion of labelled sugars to starch. For tubers with different growth rates both the total uptake of 14C from labelled photosynthate and the ratio ethanol-insoluble 14C/ethanol-soluble 14C appeared to be correlated with growth rate. In contrast when tubers were fed directly with 14C-sucrose via the tuber surface, total uptake was independent of growth rate but the correlation between growth rate and the ratio ethanol-insoluble 14C/ethanol-soluble 14C persisted. Within a particular tuber there was a decreasing gradient in sucrose synthetase activity between youngest tissue of the tuber apex and the older tissue at the tuber base but there was no clear correlation between mean enzyme activity and tuber growth rate. ADPG-pyrophosphorylase and the ratio ADPG-pyrophosphorylase/starch phosphorylase showed some correlation with tuber growth rate. Starch synthase, starch phosphorylase and UDPG-pyro-phosphorylase activities per g fresh weight of tuber tissue appeared to be relatively constant. The results suggest that the transport of sugar from the phloem sieve tubes to the tuber storage parenchyma cells, in particular the phloem unloading step, and the conversion of sugar into starch are subject to separate regulation in the potato tuber. 相似文献
14.
Li Li Maria-João Paulo Fred van Eeuwijk Christiane Gebhardt 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(7):1303-1310
Association mapping using DNA-based markers is a novel tool in plant genetics for the analysis of complex traits. Potato tuber
yield, starch content, starch yield and chip color are complex traits of agronomic relevance, for which carbohydrate metabolism
plays an important role. At the functional level, the genes and biochemical pathways involved in carbohydrate metabolism are
among the best studied in plants. Quantitative traits such as tuber starch and sugar content are therefore models for association
genetics in potato based on candidate genes. In an association mapping experiment conducted with a population of 243 tetraploid
potato varieties and breeding clones, we previously identified associations between individual candidate gene alleles and
tuber starch content, starch yield and chip quality. In the present paper, we tested 190 DNA markers at 36 loci scored in
the same association mapping population for pairwise statistical epistatic interactions. Fifty marker pairs were associated
mainly with tuber starch content and/or starch yield, at a cut-off value of q ≤ 0.20 for the experiment-wide false discovery rate (FDR). Thirteen marker pairs had an FDR of q ≤ 0.10. Alleles at loci encoding ribulose-bisphosphate carboxylase/oxygenase activase (Rca), sucrose phosphate synthase (Sps) and vacuolar invertase (Pain1) were most frequently involved in statistical epistatic interactions. The largest effect on tuber starch content and starch
yield was observed for the paired alleles Pain1-8c and Rca-1a, explaining 9 and 10% of the total variance, respectively. The combination of these two alleles increased the means of tuber
starch content and starch yield. Biological models to explain the observed statistical epistatic interactions are discussed. 相似文献
15.
马铃薯表观淀粉含量与直链淀粉含量相关性研究 总被引:4,自引:0,他引:4
测定了48个不同马铃薯品种表观淀粉含量以及块茎中和淀粉中直链淀粉含量,对表观淀粉含量和块茎中直链淀粉含量间,表观淀粉含量和淀粉粒直链淀粉含量间进行了相关分析。结果表明:表观淀粉含量和块茎中直链淀粉含量间相关显著,表观淀粉含量和淀粉粒直链淀粉含量间相关不显著,且中熟和晚熟基因型表观淀粉含量和淀粉粒直链淀粉含量间相关也不显著,这些结论将为淀粉生物合成的理论研究和淀粉品质改良提供基本的表型数据。 相似文献
16.
Potato (Solanum tuberosum L.) plants were grown under long days(LD) of 18 h before a subset of the plants was transferred to10-h photosynthetic periods with either a dark night (SD) oran 8-h dim photoperiod extension with incandescent lamps (DE).Temperature was constant at 21 °C. Leaves were sampled atthe beginning and end of the high density light period for starchanalyses. Potato leaves accumulated starch more rapidly underSD than under LD; and this difference continued after a secondmajor sink, the tuber, began to develop. Starch accumulationover 10 h in SD leaves was three times higher than in LD leaves,even after 17 d of treatment. By this time SD gave higher wholeplant relative growth rates than LD, and the tuber mass of SDplants exceeded 30% of their total plant biomass. The DE treatmentresulted in starch accumulation intermediate to the LD and SDtreatments. Genotypes likewise differed: the earlier genotype,more strongly induced to tuberize, had higher leaf starch accumulationthan the later genotype. The effects of photoperiod and genotypewere also present when potatoes were grown at 27 °C, a temperatureunfavourable for tuberization under LD. Thus the formation ofa strong tuber sink was consistently associated with more rapidleaf starch accumulation. Potato, Solanum tuberosum L., cv. Norchip, photoperiod, temperature, genotype, starch accumulation, partitionin 相似文献
17.
Turesson H Marttila S Gustavsson KE Hofvander P Olsson ME Bülow L Stymne S Carlsson AS 《American journal of botany》2010,97(11):1884-1893
? Premise of the study: Storage oil (triacylglycerol) accumulates in tissues such as the embryo and endosperm of seeds and the fruit mesocarp, but seldom in underground organs. As a rare exception, cultivated variants of yellow nutsedge (Cyperus esculentus) contain high amounts of both oil and starch in the mature tubers. ? Methods: Biochemical analyses and light and electron microscopy were used to study the accumulation patterns of storage nutrients in developing nutsedge tubers. ? Key results: During the initial phase of tuber development, the conducting rhizome tissue is transformed into a storage compartment, then massive storage reserves accumulate in the tuber. At the beginning of tuber development, a large sugar load coincided with the onset of starch accumulation. Oil accumulation started later, concomitant with a substantial drop in the sugar content. Initially, oil accumulated at a lower rate compared to starch, but the rate later increased; after 6 wk, oil made up 24% of tuber dry mass, while starch made up 32%. Protein concentration changed only a small amount throughout this development. Oil and starch accumulated in the same cells throughout the tubers in a sequential fashion during tuber development. ? Conclusions: The developmental pattern in the build up of storage nutrients in the tubers highlights nutsedge as a novel model plant, having potential to significantly widen our understanding on how synthesis of storage reserves, and in particular oils, is regulated and directed in nonseed tissues such as tubers and roots. 相似文献
18.
Natural DNA variation at candidate loci is associated with potato chip color, tuber starch content, yield and starch yield 总被引:1,自引:0,他引:1
Li L Paulo MJ Strahwald J Lübeck J Hofferbert HR Tacke E Junghans H Wunder J Draffehn A van Eeuwijk F Gebhardt C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(8):1167-1181
Complex characters of plants such as starch and sugar content of seeds, fruits, tubers and roots are controlled by multiple
genetic and environmental factors. Understanding their molecular basis will facilitate diagnosis and combination of superior
alleles in crop improvement programs (“precision breeding”). Association genetics based on candidate genes is one approach
toward this goal. Tetraploid potato varieties and breeding clones related by descent were evaluated for 2 years for chip
quality before and after cold storage, tuber starch content, yield and starch yield. Chip quality is inversely correlated
with tuber sugar content. A total of 36 loci on 11 potato chromosomes were evaluated for natural DNA variation in 243 individuals.
These loci included microsatellites and genes coding for enzymes that function in carbohydrate metabolism or transport (candidate
loci). The markers were used to analyze population structure and were tested for association with the tuber quality traits.
Highly significant and robust associations of markers with 1–4 traits were identified. Most frequent were associations with
chip quality and tuber starch content. Alleles increasing tuber starch content improved chip quality and vice versa. With
two exceptions, the most significant and robust associations (q < 0.01) were observed with DNA variants in genes encoding enzymes that function in starch and sugar metabolism or transport.
Comparing linkage and linkage disequilibrium between loci provided evidence for the existence of large haplotype blocks in
the breeding materials analyzed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
19.
Characterization of the early events of potato tuber development 总被引:1,自引:0,他引:1
David J. Hannapel 《Physiologia plantarum》1991,83(4):568-573
The early events of potato ( Solanum tuberosum L. cv. Superior) tuberization were examined by using a model system of axillary bud tuber development from petiole-leaf single-node cuttings. Both fresh weight and starch accumulation were monitored to establish a developmental framework for morphological changes. Fresh weight and starch content began to increase in axillary buds after 2 days. Visible changes in bud morphology could be detected 4 days after the start of incubation. Substantial increases in both total protein and total RNA were observed at the onset of tuber morphology. Immunoblot analysis showed that the major tuber protein, patatin, could be initially detected in day 4 buds and that a 22-kDa proteinase inhibitor could be initially detected at day 8. Northern blot analysis corroborated this pattern of accumulation at the RNA level for both protein types. Substantial accumulation of the two proteinase inhibitor mRNAs occurred later than patatin mRNA accumulation. The results of this study showed that there is considerable accumulation of both protein and mRNA occurring during the early stages of tuber development prior to the substantial accumulation of the major tuber storage proteins. 相似文献
20.
为探讨秸秆还田下旱作马铃薯块茎形成过程中淀粉合成关键酶活性及基因表达特性,以马铃薯栽培品种"青薯9号"为材料,以露地栽培为对照,设置秸秆还田处理,研究了马铃薯块茎形成过程中淀粉合成关键酶活性、基因表达、淀粉糊化及累积指标。结果表明:秸秆还田显著提高了旱作马铃薯SSS酶活性,降低了AGPP、GBSS酶活性,而对SBE酶活性没有显著影响;显著提高了SSⅡ、SSⅢ基因表达量,降低了AGPase、GBSSⅠ、SBEⅠ、SBEⅡ基因表达量;显著增加了淀粉崩解值,减少了淀粉各阶段粘度、回生值,而对淀粉糊化温度没有显著影响;显著增加了直链淀粉含量及直/支链淀粉比,减少了总淀粉含量; GBSS酶活性与AGPase、SBEⅠ基因表达量呈显著正相关,与直链淀粉含量、直/支链淀粉比呈显著负相关; SBE酶活性与SSⅡ基因表达量、峰值粘度、低谷粘度、最终粘度、总淀粉含量呈显著正相关,与崩解值、糊化温度呈显著负相关; AGPase基因表达量与直链淀粉含量呈显著负相关;GBSSⅠ基因表达量与最终粘度、回生值呈显著正相关,与糊化温度呈显著负相关;淀粉糊化与累积无显著相关性。 相似文献