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UAP56 is a conserved crucial component of a divergent mRNA export pathway in Toxoplasma gondii
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Mariana Serpeloni Elena Jiménez‐Ruiz Newton Medeiros Vidal Constanze Kroeber Nicole Andenmatten Leandro Lemgruber Patricia Mörking Gurman S. Pall Markus Meissner Andréa R. Ávila 《Molecular microbiology》2016,102(4):672-689
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Feedback control of Campylobacter jejuni flagellin levels through reciprocal binding of FliW to flagellin and the global regulator CsrA 总被引:1,自引:0,他引:1
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Katarzyna A. Radomska Soledad R. Ordoñez Marc M. S. M. Wösten Jaap A. Wagenaar Jos P. M. van Putten 《Molecular microbiology》2016,102(2):207-220
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PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes
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Liye Zhang Francois M Sement Takuma Suematsu Tian Yu Stefano Monti Lan Huang Ruslan Aphasizhev Inna Aphasizheva 《The EMBO journal》2017,36(16):2435-2454
In Trypanosoma brucei, most mitochondrial mRNAs undergo internal changes by RNA editing and 3′ end modifications. The temporally separated and functionally distinct modifications are manifested by adenylation prior to editing, and by post‐editing extension of a short A‐tail into a long A/U‐heteropolymer. The A‐tail stabilizes partially and fully edited mRNAs, while the A/U‐tail enables mRNA binding to the ribosome. Here, we identify an essential pentatricopeptide repeat‐containing RNA binding protein, kinetoplast polyadenylation factor 3 (KPAF3), and demonstrate its role in protecting pre‐mRNA against degradation by the processome. We show that KPAF3 recruits KPAP1 poly(A) polymerase to the 3′ terminus, thus leading to pre‐mRNA stabilization, or decay depending on the occurrence and extent of editing. In vitro, KPAF3 stimulates KPAP1 activity and inhibits mRNA uridylation by RET1 TUTase. Our findings indicate that KPAF3 selectively directs pre‐mRNA toward adenylation rather than uridylation, which is a default post‐trimming modification characteristic of ribosomal and guide RNAs. As a quality control mechanism, KPAF3 binding ensures that mRNAs entering the editing pathway are adenylated and, therefore, competent for post‐editing A/U‐tailing and translational activation. 相似文献